BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115208.2.1
(1469 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow st... 178 3e-043
gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow st... 178 3e-043
gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. v... 178 3e-043
gb|CB880252.1|CB880252 HM05P11w HM Hordeum vulgare subsp. v... 103 2e-020
gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm libra... 72 6e-011
gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm librar... 72 6e-011
gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm librar... 72 6e-011
gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm librar... 72 6e-011
gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm libra... 72 6e-011
gb|CA027712.1|CA027712 HZ59M12r HZ Hordeum vulgare subsp. v... 58 9e-007
gb|BM442355.2|BM442355 EBan01_SQ002_P11_R anther, yellow st... 50 2e-004
gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots... 42 0.051
gb|AL506291.1|AL506291 AL506291 Hordeum vulgare Barke devel... 40 0.20
gb|BE215274.2|BE215274 HV_CEb0006F16f Hordeum vulgare seedl... 40 0.20
gb|BE194452.3|BE194452 HVSMEh0085J23f Hordeum vulgare 5-45 ... 40 0.20
gb|BM372591.2|BM372591 EBpi05_SQ003_G09_R pistil, 8 DPA, no... 40 0.20
gb|BQ765244.1|BQ765244 EBro03_SQ006_J16_R root, 3 week, wat... 40 0.20
gb|CV060925.1|CV060925 BNEL62h11 Barley EST endosperm libra... 40 0.20
gb|CK123080.1|CK123080 BES1824102a19 BES1824 Hordeum vulgar... 40 0.20
>gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_D11 5', mRNA sequence
Length = 676
Score = 178 bits (90), Expect = 3e-043
Identities = 117/126 (92%)
Strand = Plus / Plus
Query: 336 gccggctgggcctggtccatcaaccaggccaggtcgttccgctggaacctgacggccagc 395
||||| ||||| ||||||||||||||| ||||| ||||||||||||||||||||||||
Sbjct: 281 gccggatgggcatggtccatcaaccagtggaggtccttccgctggaacctgacggccagc 340
Query: 396 gccgcgcggcccaacccgcagggctcctaccactacggccagatcaacatcacccgcacc 455
||||| |||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 341 gccgccaggcccaacccgcaggggtcctaccactacggccagatcaacatcacccgcacc 400
Query: 456 atcaag 461
||||||
Sbjct: 401 atcaag 406
Score = 93.7 bits (47), Expect = 2e-017
Identities = 95/111 (85%)
Strand = Plus / Plus
Query: 78 ctcaacttccgcatccagggccacgacatgaagctcgtcgagctggagggctcacatacc 137
|||||| |||||||||||||||| | | | ||||| || ||| |||||||||| ||||||
Sbjct: 26 ctcaacgtccgcatccagggccatgtcgtcaagctggtggagatggagggctcccatacc 85
Query: 138 ctgcaggacgtgtacgactcgctggacgtgcacgtcggccactgcctctcc 188
||||| ||| |||||||||||| ||||| ||| ||||||| |||||||||
Sbjct: 86 gtgcagaacgagtacgactcgctcgacgtccacatcggccagtgcctctcc 136
Score = 69.9 bits (35), Expect = 2e-010
Identities = 77/91 (84%)
Strand = Plus / Plus
Query: 641 ccccaatgtcatcaccgccgagttccgcaccttcatcgagatcgtcttcgagaaccccga 700
|||||| ||||||||||||||| |||||||| ||| || ||||||| |||||||||||
Sbjct: 586 ccccaacgtcatcaccgccgagcaccgcacctacattgaagtcgtctttgagaaccccga 645
Query: 701 gaagagcatagactccctccacgtcgacggc 731
|| |||||| ||| || ||||| || |||||
Sbjct: 646 gaggagcatcgacaccttccacctcaacggc 676
Score = 58.0 bits (29), Expect = 9e-007
Identities = 38/41 (92%)
Strand = Plus / Plus
Query: 532 ccgagacccccgtgaagctcgccgagtacttcaacgccacc 572
||||||| ||| | |||||||||||||||||||||||||||
Sbjct: 477 ccgagacgcccctcaagctcgccgagtacttcaacgccacc 517
>gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G14 5', mRNA sequence
Length = 341
Score = 178 bits (90), Expect = 3e-043
Identities = 117/126 (92%)
Strand = Plus / Plus
Query: 336 gccggctgggcctggtccatcaaccaggccaggtcgttccgctggaacctgacggccagc 395
||||| ||||| ||||||||||||||| ||||| ||||||||||||||||||||||||
Sbjct: 62 gccggatgggcatggtccatcaaccagtggaggtccttccgctggaacctgacggccagc 121
Query: 396 gccgcgcggcccaacccgcagggctcctaccactacggccagatcaacatcacccgcacc 455
||||| |||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 122 gccgccaggcccaacccgcaggggtcctaccactacggccagatcaacatcacccgcacc 181
Query: 456 atcaag 461
||||||
Sbjct: 182 atcaag 187
Score = 58.0 bits (29), Expect = 9e-007
Identities = 38/41 (92%)
Strand = Plus / Plus
Query: 532 ccgagacccccgtgaagctcgccgagtacttcaacgccacc 572
||||||| ||| | |||||||||||||||||||||||||||
Sbjct: 258 ccgagacgcccctcaagctcgccgagtacttcaacgccacc 298
>gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. vulgare cDNA clone HM14F23
5-PRIME, mRNA sequence
Length = 628
Score = 178 bits (90), Expect = 3e-043
Identities = 117/126 (92%)
Strand = Plus / Plus
Query: 336 gccggctgggcctggtccatcaaccaggccaggtcgttccgctggaacctgacggccagc 395
||||| ||||| ||||||||||||||| ||||| ||||||||||||||||||||||||
Sbjct: 422 gccggatgggcatggtccatcaaccagtggaggtccttccgctggaacctgacggccagc 481
Query: 396 gccgcgcggcccaacccgcagggctcctaccactacggccagatcaacatcacccgcacc 455
||||| |||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 482 gccgccaggcccaacccgcaggggtcctaccactacggccagatcaacatcacccgcacc 541
Query: 456 atcaag 461
||||||
Sbjct: 542 atcaag 547
Score = 117 bits (59), Expect = 1e-024
Identities = 134/159 (84%)
Strand = Plus / Plus
Query: 30 ggcaagacgtaccgcctccgcgtgtgcaacaccgggatcaaggcgtcgctcaacttccgc 89
||||||||||||||| ||||||| |||||| ||| |||||| | | |||||| |||||
Sbjct: 119 ggcaagacgtaccgcttccgcgtctgcaacgtcggcatcaagaccaccctcaacgtccgc 178
Query: 90 atccagggccacgacatgaagctcgtcgagctggagggctcacataccctgcaggacgtg 149
||||||||||| | | | ||||| || ||| |||||||||| |||||| ||||| ||| |
Sbjct: 179 atccagggccatgtcgtcaagctggtggagatggagggctcccataccgtgcagaacgag 238
Query: 150 tacgactcgctggacgtgcacgtcggccactgcctctcc 188
||||||||||| ||||| ||| ||||||| |||||||||
Sbjct: 239 tacgactcgctcgacgtccacatcggccagtgcctctcc 277
>gb|CB880252.1|CB880252 HM05P11w HM Hordeum vulgare subsp. vulgare cDNA clone HM05P11
3-PRIME, mRNA sequence
Length = 664
Score = 103 bits (52), Expect = 2e-020
Identities = 134/160 (83%), Gaps = 1/160 (0%)
Strand = Plus / Plus
Query: 30 ggcaagacgtaccgcctccgcgtgtgcaacaccgggatcaagg-cgtcgctcaacttccg 88
||||||||||||||| ||||||| |||||| ||| |||||| | | |||||| ||||
Sbjct: 375 ggcaagacgtaccgcttccgcgtctgcaacgtcggcatcaagatccaccctcaacgtccg 434
Query: 89 catccagggccacgacatgaagctcgtcgagctggagggctcacataccctgcaggacgt 148
|||||||||||| | | | ||||| || ||| |||||||||| |||||| ||||| |||
Sbjct: 435 catccagggccatgtcgtcaagctggtggagatggagggctcccataccgtgcagaacga 494
Query: 149 gtacgactcgctggacgtgcacgtcggccactgcctctcc 188
|||||||||||| ||||| ||| ||||||| |||||||||
Sbjct: 495 gtacgactcgctcgacgtccacatcggccagtgcctctcc 534
>gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL117e7 5' similar to Oryza sativa,
mRNA sequence
Length = 674
Score = 71.9 bits (36), Expect = 6e-011
Identities = 69/80 (86%)
Strand = Plus / Plus
Query: 380 gaacctgacggccagcgccgcgcggcccaacccgcagggctcctaccactacggccagat 439
|||||||||||| |||| ||||||| |||||||||||||| |||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 440 caacatcacccgcaccatca 459
|||| | | |||||||||||
Sbjct: 271 caacgtgagccgcaccatca 290
>gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL37h7 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 568
Score = 71.9 bits (36), Expect = 6e-011
Identities = 69/80 (86%)
Strand = Plus / Plus
Query: 380 gaacctgacggccagcgccgcgcggcccaacccgcagggctcctaccactacggccagat 439
|||||||||||| |||| ||||||| |||||||||||||| |||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 440 caacatcacccgcaccatca 459
|||| | | |||||||||||
Sbjct: 271 caacgtgagccgcaccatca 290
>gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL49f3 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 502
Score = 71.9 bits (36), Expect = 6e-011
Identities = 69/80 (86%)
Strand = Plus / Plus
Query: 380 gaacctgacggccagcgccgcgcggcccaacccgcagggctcctaccactacggccagat 439
|||||||||||| |||| ||||||| |||||||||||||| |||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 440 caacatcacccgcaccatca 459
|||| | | |||||||||||
Sbjct: 271 caacgtgagccgcaccatca 290
>gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL82f4 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 581
Score = 71.9 bits (36), Expect = 6e-011
Identities = 69/80 (86%)
Strand = Plus / Plus
Query: 380 gaacctgacggccagcgccgcgcggcccaacccgcagggctcctaccactacggccagat 439
|||||||||||| |||| ||||||| |||||||||||||| |||||||||||| |||
Sbjct: 483 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 542
Query: 440 caacatcacccgcaccatca 459
|||| | | |||||||||||
Sbjct: 543 caacgtgagccgcaccatca 562
Score = 42.1 bits (21), Expect = 0.051
Identities = 45/53 (84%)
Strand = Plus / Plus
Query: 76 cgctcaacttccgcatccagggccacgacatgaagctcgtcgagctggagggc 128
||||||||||| |||||||||||| || ||| |||||| ||| ||||||||
Sbjct: 76 cgctcaacttcatgatccagggccacaacgtgacgctcgtggaggtggagggc 128
>gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL89d11 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 593
Score = 71.9 bits (36), Expect = 6e-011
Identities = 69/80 (86%)
Strand = Plus / Plus
Query: 380 gaacctgacggccagcgccgcgcggcccaacccgcagggctcctaccactacggccagat 439
|||||||||||| |||| ||||||| |||||||||||||| |||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 440 caacatcacccgcaccatca 459
|||| | | |||||||||||
Sbjct: 271 caacgtgagccgcaccatca 290
>gb|CA027712.1|CA027712 HZ59M12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59M12
5-PRIME, mRNA sequence
Length = 264
Score = 58.0 bits (29), Expect = 9e-007
Identities = 35/37 (94%)
Strand = Plus / Plus
Query: 139 tgcaggacgtgtacgactcgctggacgtgcacgtcgg 175
||||| ||||||||||||| |||||||||||||||||
Sbjct: 184 tgcagaacgtgtacgactccctggacgtgcacgtcgg 220
Score = 46.1 bits (23), Expect = 0.003
Identities = 35/39 (89%)
Strand = Plus / Plus
Query: 62 cgggatcaaggcgtcgctcaacttccgcatccagggcca 100
|||||| ||| ||||| |||||||||| |||||||||||
Sbjct: 107 cgggatgaagacgtcgatcaacttccggatccagggcca 145
>gb|BM442355.2|BM442355 EBan01_SQ002_P11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_P11 5', mRNA sequence
Length = 620
Score = 50.1 bits (25), Expect = 2e-004
Identities = 46/53 (86%)
Strand = Plus / Plus
Query: 7 cgcccatgctcaccttcgaggcgggcaagacgtaccgcctccgcgtgtgcaac 59
|||||||| |||||| ||||| | ||||||||||||||||||| | ||||||
Sbjct: 553 cgcccatgttcacctgggaggccgccaagacgtaccgcctccgcatctgcaac 605
>gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06V 5', mRNA sequence
Length = 694
Score = 42.1 bits (21), Expect = 0.051
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 724 tcgacggctacgccttcttcggcgtcgggatgg 756
|||||||||||||||||| | |||||||||||
Sbjct: 136 tcgacggctacgccttctgggtcgtcgggatgg 168
>gb|AL506291.1|AL506291 AL506291 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02K09T 5',
mRNA sequence
Length = 700
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 189 cgacggcaagctccgctacg 208
>gb|BE215274.2|BE215274 HV_CEb0006F16f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0006F16f, mRNA sequence
Length = 473
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 147 cgacggcaagctccgctacg 166
>gb|BE194452.3|BE194452 HVSMEh0085J23f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0085J23f, mRNA sequence
Length = 896
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 93 cgacggcaagctccgctacg 112
>gb|BM372591.2|BM372591 EBpi05_SQ003_G09_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_G09 5', mRNA sequence
Length = 540
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 167 cgacggcaagctccgctacg 186
>gb|BQ765244.1|BQ765244 EBro03_SQ006_J16_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_J16 5', mRNA sequence
Length = 544
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 43 cgacggcaagctccgctacg 62
>gb|CV060925.1|CV060925 BNEL62h11 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL62h11 5' similar to mago
nashi-like protein, mRNA sequence
Length = 554
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 165 cgacggcaagctccgctacg 184
>gb|CK123080.1|CK123080 BES1824102a19 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A192 5-PRIME, mRNA sequence
Length = 588
Score = 40.1 bits (20), Expect = 0.20
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 485 cgacggcaagctccgctacg 504
||||||||||||||||||||
Sbjct: 37 cgacggcaagctccgctacg 56
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 199,474
Number of Sequences: 312970
Number of extensions: 199474
Number of successful extensions: 62585
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 62541
Number of HSP's gapped (non-prelim): 44
length of query: 1469
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1450
effective length of database: 169,188,109
effective search space: 245322758050
effective search space used: 245322758050
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)