BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071743.2.1
(586 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621611.1|BF621611 HVSMEa0011J15f Hordeum vulgare seedl... 84 6e-015
gb|BJ453003.1|BJ453003 BJ453003 K. Sato unpublished cDNA li... 62 2e-008
gb|BM099579.2|BM099579 EBes01_SQ003_K13_R embryo sac, 4-6 D... 62 2e-008
gb|BU997001.1|BU997001 HI06J07r HI Hordeum vulgare subsp. v... 62 2e-008
gb|BM817109.1|BM817109 HC02A08_T3.ab1 HC Hordeum vulgare su... 60 9e-008
gb|BI959387.1|BI959387 HVSMEn0019H17f Hordeum vulgare rachi... 50 8e-005
gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. v... 48 3e-004
gb|BF625895.2|BF625895 HVSMEa0014B15f Hordeum vulgare seedl... 44 0.005
>gb|BF621611.1|BF621611 HVSMEa0011J15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011J15f, mRNA sequence
Length = 868
Score = 83.8 bits (42), Expect = 6e-015
Identities = 75/86 (87%)
Strand = Plus / Plus
Query: 487 gaaaaagcaactcccaaaggtgtgctgaaactaatgaaggtagaaggtctaacaatttat 546
|||||||| |||||||| ||||||||||| || ||||| || || ||| | ||||| |||
Sbjct: 113 gaaaaagctactcccaagggtgtgctgaagcttatgaaagttgacggtttgacaatatat 172
Query: 547 catgtgaagagccatctgcagaagta 572
|||||||| |||||||||||||||||
Sbjct: 173 catgtgaaaagccatctgcagaagta 198
>gb|BJ453003.1|BJ453003 BJ453003 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41e01 5', mRNA sequence
Length = 602
Score = 61.9 bits (31), Expect = 2e-008
Identities = 43/47 (91%)
Strand = Plus / Plus
Query: 538 acaatttatcatgtgaagagccatctgcagaagtatcggcttgcaaa 584
||||| ||||| |||||||||||| ||||||||||||| ||||||||
Sbjct: 46 acaatctatcacgtgaagagccatttgcagaagtatcgccttgcaaa 92
>gb|BM099579.2|BM099579 EBes01_SQ003_K13_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_K13 5', mRNA sequence
Length = 602
Score = 61.9 bits (31), Expect = 2e-008
Identities = 43/47 (91%)
Strand = Plus / Plus
Query: 538 acaatttatcatgtgaagagccatctgcagaagtatcggcttgcaaa 584
||||| ||||| |||||||||||| ||||||||||||| ||||||||
Sbjct: 328 acaatctatcacgtgaagagccatttgcagaagtatcgccttgcaaa 374
>gb|BU997001.1|BU997001 HI06J07r HI Hordeum vulgare subsp. vulgare cDNA clone HI06J07
5-PRIME, mRNA sequence
Length = 542
Score = 61.9 bits (31), Expect = 2e-008
Identities = 43/47 (91%)
Strand = Plus / Plus
Query: 538 acaatttatcatgtgaagagccatctgcagaagtatcggcttgcaaa 584
||||| ||||| |||||||||||| ||||||||||||| ||||||||
Sbjct: 372 acaatctatcacgtgaagagccatttgcagaagtatcgccttgcaaa 418
>gb|BM817109.1|BM817109 HC02A08_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC02A08_T3.ab1 similar to (AF219972) CDPK substrate
protein 1; CSP1 [Mesembryanthemum crystallinum], mRNA
sequence
Length = 828
Score = 60.0 bits (30), Expect = 9e-008
Identities = 75/90 (83%)
Strand = Plus / Plus
Query: 486 tgaaaaagcaactcccaaaggtgtgctgaaactaatgaaggtagaaggtctaacaattta 545
||||||||||||||| |||| || | |||| | |||||||| ||||| ||||| || ||
Sbjct: 231 tgaaaaagcaactccaaaagcagtccagaaagttatgaaggttgaagggctaactatata 290
Query: 546 tcatgtgaagagccatctgcagaagtatcg 575
||||| || |||||||| |||||||||||
Sbjct: 291 ccatgtaaaaagccatctacagaagtatcg 320
>gb|BI959387.1|BI959387 HVSMEn0019H17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0019H17f, mRNA sequence
Length = 648
Score = 50.1 bits (25), Expect = 8e-005
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 538 acaatttatcatgtgaagagccatctgcagaagtatcggcttgca 582
||||| ||||| |||||||||||| ||||||||||| | ||||||
Sbjct: 450 acaatctatcacgtgaagagccatttgcagaagtattgccttgca 494
>gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. vulgare cDNA clone HM04L11
5-PRIME, mRNA sequence
Length = 651
Score = 48.1 bits (24), Expect = 3e-004
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 559 catctgcagaagtatcggcttgcaaaat 586
||||||||||| ||||||||||||||||
Sbjct: 19 catctgcagaaatatcggcttgcaaaat 46
>gb|BF625895.2|BF625895 HVSMEa0014B15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014B15f, mRNA sequence
Length = 486
Score = 44.1 bits (22), Expect = 0.005
Identities = 64/78 (82%)
Strand = Plus / Plus
Query: 486 tgaaaaagcaactcccaaaggtgtgctgaaactaatgaaggtagaaggtctaacaattta 545
||||||||||||||| |||| || | |||| | |||||||| ||||| ||||| || ||
Sbjct: 275 tgaaaaagcaactccaaaagcagtccagaaagttatgaaggttgaagggctaactatata 334
Query: 546 tcatgtgaagagccatct 563
||||| || ||||||||
Sbjct: 335 ccatgtaaaaagccatct 352
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 52,897
Number of Sequences: 312970
Number of extensions: 52897
Number of successful extensions: 13622
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13613
Number of HSP's gapped (non-prelim): 9
length of query: 586
length of database: 175,134,539
effective HSP length: 19
effective length of query: 567
effective length of database: 169,188,109
effective search space: 95929657803
effective search space used: 95929657803
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)