BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067516.2.1
(605 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ463876.1|BJ463876 BJ463876 K. Sato unpublished cDNA li... 125 2e-027
gb|CB880830.1|CB880830 HM07P10w HM Hordeum vulgare subsp. v... 125 2e-027
gb|BU971323.1|BU971323 HB17E13r BC Hordeum vulgare subsp. v... 111 3e-023
gb|BJ468047.1|BJ468047 BJ468047 K. Sato unpublished cDNA li... 100 1e-019
gb|BQ753962.1|BQ753962 EBca01_SQ002_G09_R carpel, pre-anthe... 72 2e-011
gb|CB881377.1|CB881377 HM09K03w HM Hordeum vulgare subsp. v... 48 3e-004
>gb|BJ463876.1|BJ463876 BJ463876 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n18 5', mRNA sequence
Length = 680
Score = 125 bits (63), Expect = 2e-027
Identities = 165/199 (82%)
Strand = Plus / Minus
Query: 407 gatgatatctcttctcaactttgtcactttgttaatgatcgcctctatggaaacatcaaa 466
|||||||||||||||||| | |||||||||||| ||||| ||| | |||||||||||
Sbjct: 662 gatgatatctcttctcaattgtgtcactttgttgatgattgccgaaaccgaaacatcaaa 603
Query: 467 tgcatctttcaatgtctccagtttcgatcttggatcggcatatatcacctttggtatcag 526
|| || || | || ||||||||||| |||||||| |||||||||||||||||||| ||
Sbjct: 602 cgcgtcctttagcgtttccagtttcgaccttggatcagcatatatcacctttggtatgag 543
Query: 527 attgatgacctcctctctcatttgcttgaccacatctgggtgaatactctttagcctctc 586
|||||| ||||| || ||||| |||||||| || ||||| || ||||| |||| ||||
Sbjct: 542 attgataacctcttccctcatctgcttgacaacgtctggatggatacttcttagtatctc 483
Query: 587 ctcaatgctggcatttcca 605
||||| ||| ||||||||
Sbjct: 482 ctcaacactgacatttcca 464
>gb|CB880830.1|CB880830 HM07P10w HM Hordeum vulgare subsp. vulgare cDNA clone HM07P10
3-PRIME, mRNA sequence
Length = 628
Score = 125 bits (63), Expect = 2e-027
Identities = 235/293 (80%), Gaps = 12/293 (4%)
Strand = Plus / Plus
Query: 232 ttttccaagaattcttggcagcttctgcagatgaaccactagaatctccacctttatctt 291
||||||||||||||||||| ||||| || |||||| || ||||||| |||| | ||||
Sbjct: 342 ttttccaagaattcttggctgcttcagctgatgaattaccagaatctgcacccatgtctt 401
Query: 292 taggctt------cgagaagaatggatcccatttgtgaggtccaattgcctgctgtccat 345
| ||||| |||||||||||||||||| | |||||||| |||| | |||||||
Sbjct: 402 tgggcttgggcttcgagaagaatggatcccactcatgaggtccgattgtccgctgtcc-- 459
Query: 346 cgtctaacagactatacttccagctattctcttcgataaaatctttgtcttcttggcctg 405
|||||| ||||||||||||||||||||| ||||||||||| ||||| | | |
Sbjct: 460 ----taacagttcatacttccagctattctcttcaataaaatctttatcttcgctgtcgg 515
Query: 406 cgatgatatctcttctcaactttgtcactttgttaatgatcgcctctatggaaacatcaa 465
|||||||||||||||||| | |||||||||||| ||||| ||| | ||||||||||
Sbjct: 516 agatgatatctcttctcaattgtgtcactttgttgatgattgccgaaaccgaaacatcaa 575
Query: 466 atgcatctttcaatgtctccagtttcgatcttggatcggcatatatcaccttt 518
| || || || | || ||||||||||| |||||||| |||||||||||||||
Sbjct: 576 acgcgtcctttagcgtttccagtttcgaccttggatcagcatatatcaccttt 628
>gb|BU971323.1|BU971323 HB17E13r BC Hordeum vulgare subsp. vulgare cDNA clone HB17E13
5-PRIME, mRNA sequence
Length = 519
Score = 111 bits (56), Expect = 3e-023
Identities = 165/200 (82%), Gaps = 1/200 (0%)
Strand = Plus / Minus
Query: 407 gatgatatctcttctcaactttgtcactttgttaatgatcgcctctatggaaa-catcaa 465
|||||||||||||||||| | |||||||||||| ||||| ||| | |||| ||||||
Sbjct: 509 gatgatatctcttctcaattgtgtcactttgttgatgattgccgaaaccgaaaacatcaa 450
Query: 466 atgcatctttcaatgtctccagtttcgatcttggatcggcatatatcacctttggtatca 525
| || || || | || ||||||||||| |||||||| |||||||||||||||||||| |
Sbjct: 449 acgcgtcctttagcgtttccagtttcgaccttggatcagcatatatcacctttggtatga 390
Query: 526 gattgatgacctcctctctcatttgcttgaccacatctgggtgaatactctttagcctct 585
||||||| ||||| || ||||| |||||||| || ||||| || ||||| |||| |||
Sbjct: 389 gattgataacctcttccctcatctgcttgacaacgtctggatggatacttcttagtatct 330
Query: 586 cctcaatgctggcatttcca 605
|||||| ||| ||||||||
Sbjct: 329 cctcaacactgacatttcca 310
>gb|BJ468047.1|BJ468047 BJ468047 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n18 3', mRNA sequence
Length = 573
Score = 99.6 bits (50), Expect = 1e-019
Identities = 177/220 (80%), Gaps = 12/220 (5%)
Strand = Plus / Plus
Query: 232 ttttccaagaattcttggcagcttctgcagatgaaccactagaatctccacctttatctt 291
||||||||||||||||||| ||||| || |||||| || ||||||| |||| | ||||
Sbjct: 343 ttttccaagaattcttggctgcttcagctgatgaattaccagaatctgcacccatgtctt 402
Query: 292 taggctt------cgagaagaatggatcccatttgtgaggtccaattgcctgctgtccat 345
| ||||| |||||||||||||||||| | |||||||| |||| | |||||||
Sbjct: 403 tgggcttgggcttcgagaagaatggatcccactcatgaggtccgattgtccgctgtcc-- 460
Query: 346 cgtctaacagactatacttccagctattctcttcgataaaatctttgtcttcttggcctg 405
|||||| ||||||||||||||||||||| ||||||||||| ||||| | | |
Sbjct: 461 ----taacagttcatacttccagctattctcttcaataaaatctttatcttcgctgtcgg 516
Query: 406 cgatgatatctcttctcaactttgtcactttgttaatgat 445
|||||||||||||||||| | |||||||||||| |||||
Sbjct: 517 agatgatatctcttctcaattgtgtcactttgttgatgat 556
>gb|BQ753962.1|BQ753962 EBca01_SQ002_G09_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_G09 5', mRNA sequence
Length = 504
Score = 71.9 bits (36), Expect = 2e-011
Identities = 133/166 (80%), Gaps = 12/166 (7%)
Strand = Plus / Minus
Query: 232 ttttccaagaattcttggcagcttctgcagatgaaccactagaatctccacctttatctt 291
||||||||||||||||||| ||||| || |||||| || ||||||| |||| | ||||
Sbjct: 163 ttttccaagaattcttggctgcttcagctgatgaattaccagaatctgcacccatgtctt 104
Query: 292 taggctt------cgagaagaatggatcccatttgtgaggtccaattgcctgctgtccat 345
| ||||| |||||||||||||||||| | |||||||| |||| | |||||||
Sbjct: 103 tgggcttgggcttcgagaagaatggatcccactcatgaggtccgattgtccgctgtcc-- 46
Query: 346 cgtctaacagactatacttccagctattctcttcgataaaatcttt 391
|||||| ||||||||||||||||||||| |||||||||||
Sbjct: 45 ----taacagttcatacttccagctattctcttcaataaaatcttt 4
>gb|CB881377.1|CB881377 HM09K03w HM Hordeum vulgare subsp. vulgare cDNA clone HM09K03
3-PRIME, mRNA sequence
Length = 379
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 232 ttttccaagaattcttggcagcttctgcagatgaaccactagaatctccacc 283
||||||||||||||||||| ||||| || |||||| || ||||||| ||||
Sbjct: 247 ttttccaagaattcttggctgcttcagctgatgaattaccagaatctgcacc 298
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 51,659
Number of Sequences: 312970
Number of extensions: 51659
Number of successful extensions: 13167
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13140
Number of HSP's gapped (non-prelim): 23
length of query: 605
length of database: 175,134,539
effective HSP length: 19
effective length of query: 586
effective length of database: 169,188,109
effective search space: 99144231874
effective search space used: 99144231874
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)