BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067450.2.1
(724 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW982411.2|AW982411 HVSMEg0003D05f Hordeum vulgare pre-a... 80 1e-013
gb|BI956571.1|BI956571 HVSMEn0004D06f Hordeum vulgare rachi... 80 1e-013
gb|BI957606.1|BI957606 HVSMEn0010F15f Hordeum vulgare rachi... 70 1e-010
gb|BI960501.1|BI960501 HVSMEn0024N01f Hordeum vulgare rachi... 70 1e-010
gb|BG343272.2|BG343272 HVSMEg0005E13f Hordeum vulgare pre-a... 70 1e-010
gb|BF628874.2|BF628874 HVSMEb0009A12f Hordeum vulgare seedl... 66 2e-009
gb|AV913364.1|AV913364 AV913364 K. Sato unpublished cDNA li... 64 7e-009
gb|AV918663.1|AV918663 AV918663 K. Sato unpublished cDNA li... 64 7e-009
gb|BI958645.1|BI958645 HVSMEn0016A21f Hordeum vulgare rachi... 58 4e-007
gb|BI957639.1|BI957639 HVSMEn0010H12f Hordeum vulgare rachi... 50 1e-004
gb|BI959987.1|BI959987 HVSMEn0022M02f Hordeum vulgare rachi... 48 4e-004
gb|BG344915.2|BG344915 HVSMEg0016N12f Hordeum vulgare pre-a... 46 0.002
gb|BF264626.3|BF264626 HV_CEa0009O22f Hordeum vulgare seedl... 42 0.025
gb|BM817329.1|BM817329 HC105E06_T3.ab1 HC Hordeum vulgare s... 42 0.025
gb|BQ658222.1|BQ658222 HA05D15u HA Hordeum vulgare subsp. v... 42 0.025
gb|BQ659524.1|BQ659524 HE01L03u HE Hordeum vulgare subsp. v... 42 0.025
gb|BU974771.1|BU974771 HB29A24r BC Hordeum vulgare subsp. v... 42 0.025
gb|CB877071.1|CB877071 HP03H10T HP Hordeum vulgare subsp. v... 42 0.025
gb|CB879734.1|CB879734 HP03H10w HP Hordeum vulgare subsp. v... 42 0.025
gb|BM817346.1|BM817346 HC111F01_T3.ab1 HC Hordeum vulgare s... 40 0.098
gb|AV836790.1|AV836790 AV836790 K. Sato unpublished cDNA li... 38 0.39
gb|BQ134698.1|BQ134698 LP20027 Lemma/palea-enriched cDNA li... 38 0.39
gb|BQ294531.1|BQ294531 LP10296 Lemma/palea-enriched cDNA li... 38 0.39
>gb|AW982411.2|AW982411 HVSMEg0003D05f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003D05f, mRNA sequence
Length = 1041
Score = 79.8 bits (40), Expect = 1e-013
Identities = 79/92 (85%)
Strand = Plus / Minus
Query: 632 gccgccgcgccatgcccgccgccaacgtcgaccagggagcgtatcccctgaaacacgtcg 691
|||||| | ||||| || ||| |||||||||||||||||| ||| |||| || ||| |
Sbjct: 424 gccgccccaccatggcctccggcaacgtcgaccagggagcctataccctcgaagacgctg 365
Query: 692 ccgcactccctgacggcgatgtccatgaggaa 723
||||||||||||| ||||||||||||||||||
Sbjct: 364 ccgcactccctgatggcgatgtccatgaggaa 333
Score = 40.1 bits (20), Expect = 0.098
Identities = 47/56 (83%)
Strand = Plus / Minus
Query: 383 attatcaccttgccgcctgcatctcgtggaggtatagctttcttgcagttctttag 438
||||||||||| ||||| |||||| ||||| |||||||| ||||| || |||||
Sbjct: 673 attatcacctttccgccagcatcttttggagcgatagctttattgcaattttttag 618
>gb|BI956571.1|BI956571 HVSMEn0004D06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0004D06f, mRNA sequence
Length = 780
Score = 79.8 bits (40), Expect = 1e-013
Identities = 79/92 (85%)
Strand = Plus / Minus
Query: 632 gccgccgcgccatgcccgccgccaacgtcgaccagggagcgtatcccctgaaacacgtcg 691
|||||| | ||||| || ||| |||||||||||||||||| ||| |||| || ||| |
Sbjct: 442 gccgccccaccatggcctccggcaacgtcgaccagggagcctataccctcgaagacgctg 383
Query: 692 ccgcactccctgacggcgatgtccatgaggaa 723
||||||||||||| ||||||||||||||||||
Sbjct: 382 ccgcactccctgatggcgatgtccatgaggaa 351
Score = 40.1 bits (20), Expect = 0.098
Identities = 53/64 (82%)
Strand = Plus / Minus
Query: 470 tgcatgatccacttgaggaagacagcattcgctggcggaatggcctcaaacatgtcgcca 529
||||| |||||||| ||||||||| ||| || | |||||| |||||||||||||||
Sbjct: 604 tgcataatccactttaggaagacaacatctgcctgtggaatgctctcaaacatgtcgccc 545
Query: 530 gcaa 533
||||
Sbjct: 544 gcaa 541
>gb|BI957606.1|BI957606 HVSMEn0010F15f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010F15f, mRNA sequence
Length = 648
Score = 69.9 bits (35), Expect = 1e-010
Identities = 61/70 (87%)
Strand = Plus / Minus
Query: 654 caacgtcgaccagggagcgtatcccctgaaacacgtcgccgcactccctgacggcgatgt 713
|||||||||||||||||| ||| || | || ||| |||||||||||||| ||||||||
Sbjct: 639 caacgtcgaccagggagcctataccttngaagacgctgccgcactccctgatggcgatgt 580
Query: 714 ccatgaggaa 723
||||||||||
Sbjct: 579 ccatgaggaa 570
>gb|BI960501.1|BI960501 HVSMEn0024N01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024N01f, mRNA sequence
Length = 660
Score = 69.9 bits (35), Expect = 1e-010
Identities = 61/70 (87%)
Strand = Plus / Minus
Query: 654 caacgtcgaccagggagcgtatcccctgaaacacgtcgccgcactccctgacggcgatgt 713
|||||||||||||||||| ||| || | || ||| |||||||||||||| ||||||||
Sbjct: 638 caacgtcgaccagggagcctataccttngaagacgctgccgcactccctgatggcgatgt 579
Query: 714 ccatgaggaa 723
||||||||||
Sbjct: 578 ccatgaggaa 569
>gb|BG343272.2|BG343272 HVSMEg0005E13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005E13f, mRNA sequence
Length = 992
Score = 69.9 bits (35), Expect = 1e-010
Identities = 60/69 (86%)
Strand = Plus / Minus
Query: 654 caacgtcgaccagggagcgtatcccctgaaacacgtcgccgcactccctgacggcgatgt 713
||||||||| |||||||| ||| |||| || ||| |||||||||||||| ||||||||
Sbjct: 644 caacgtcgancagggagcntataccctcgaagacgctgccgcactccctgatggcgatgt 585
Query: 714 ccatgagga 722
|||||||||
Sbjct: 584 ccatgagga 576
>gb|BF628874.2|BF628874 HVSMEb0009A12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009A12f, mRNA sequence
Length = 875
Score = 65.9 bits (33), Expect = 2e-009
Identities = 162/205 (79%)
Strand = Plus / Minus
Query: 329 tgtgtctctttgttcttaaggtttggaggccctgcaccaaccacggtatctactattatc 388
||||||||||||| ||| |||| | | || ||| |||||||||| | ||||| ||||||
Sbjct: 246 tgtgtctctttgtgcttcaggtcttgcgggcctccaccaaccaccatgtctacaattatc 187
Query: 389 accttgccgcctgcatctcgtggaggtatagctttcttgcagttctttagaatcgtgaca 448
||||| ||||| |||||| ||||| |||||||| ||||| || ||||| || |||
Sbjct: 186 acctttccgccagcatcttttggagcgatagctttattgcaattttttagtattttgatg 127
Query: 449 cactcagagtcaccccagtcgtgcatgatccacttgaggaagacagcattcgctggcgga 508
|| ||| ||| | ||| || ||||| |||||||| ||||||||| ||| || | |||
Sbjct: 126 cagtcatcgtcgctccaatcatgcataatccactttaggaagacaacatctgcctgtgga 67
Query: 509 atggcctcaaacatgtcgccagcaa 533
||| ||||||||||||||| ||||
Sbjct: 66 atgctctcaaacatgtcgcccgcaa 42
>gb|AV913364.1|AV913364 AV913364 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m21 5', mRNA sequence
Length = 665
Score = 63.9 bits (32), Expect = 7e-009
Identities = 47/52 (90%)
Strand = Plus / Minus
Query: 673 tatcccctgaaacacgtcgccgcactccctgacggcgatgtccatgaggaag 724
||||||||| || || |||||||||||| |||||||||||||||||| ||||
Sbjct: 236 tatcccctggaagacctcgccgcactccttgacggcgatgtccatgatgaag 185
>gb|AV918663.1|AV918663 AV918663 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m21 3', mRNA sequence
Length = 612
Score = 63.9 bits (32), Expect = 7e-009
Identities = 47/52 (90%)
Strand = Plus / Plus
Query: 673 tatcccctgaaacacgtcgccgcactccctgacggcgatgtccatgaggaag 724
||||||||| || || |||||||||||| |||||||||||||||||| ||||
Sbjct: 389 tatcccctggaagacctcgccgcactccttgacggcgatgtccatgatgaag 440
>gb|BI958645.1|BI958645 HVSMEn0016A21f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0016A21f, mRNA sequence
Length = 624
Score = 58.0 bits (29), Expect = 4e-007
Identities = 32/33 (96%)
Strand = Plus / Minus
Query: 691 gccgcactccctgacggcgatgtccatgaggaa 723
|||||||||||||| ||||||||||||||||||
Sbjct: 609 gccgcactccctgatggcgatgtccatgaggaa 577
>gb|BI957639.1|BI957639 HVSMEn0010H12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010H12f, mRNA sequence
Length = 652
Score = 50.1 bits (25), Expect = 1e-004
Identities = 31/33 (93%)
Strand = Plus / Minus
Query: 691 gccgcactccctgacggcgatgtccatgaggaa 723
|||||||||||| | ||||||||||||||||||
Sbjct: 591 gccgcactccctaatggcgatgtccatgaggaa 559
>gb|BI959987.1|BI959987 HVSMEn0022M02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0022M02f, mRNA sequence
Length = 629
Score = 48.1 bits (24), Expect = 4e-004
Identities = 51/60 (85%)
Strand = Plus / Minus
Query: 664 cagggagcgtatcccctgaaacacgtcgccgcactccctgacggcgatgtccatgaggaa 723
|||||||| ||| |||| || |||| |||||||||| ||| || |||||||||||||||
Sbjct: 629 cagggagcttataccctcgaagacgttgccgcactccgtgatggggatgtccatgaggaa 570
>gb|BG344915.2|BG344915 HVSMEg0016N12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0016N12f, mRNA sequence
Length = 635
Score = 46.1 bits (23), Expect = 0.002
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 691 gccgcactccctgacggcgatgtccatgaggaa 723
|||||||||| ||| | ||||||||||||||||
Sbjct: 608 gccgcactccntgatgncgatgtccatgaggaa 576
>gb|BF264626.3|BF264626 HV_CEa0009O22f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0009O22f, mRNA sequence
Length = 877
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Minus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 102 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 58
>gb|BM817329.1|BM817329 HC105E06_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC105E06_T3.ab1 similar to Hexaprenyldihydroxybenzoate
methyltransferase,(S)-scoulerine 9-O-methyltransferase,
mRNA sequence
Length = 885
Score = 42.1 bits (21), Expect = 0.025
Identities = 24/25 (96%)
Strand = Plus / Minus
Query: 514 ctcaaacatgtcgccagcaacgaag 538
||||||||||||||||| |||||||
Sbjct: 449 ctcaaacatgtcgccaggaacgaag 425
Score = 38.2 bits (19), Expect = 0.39
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 581 aggtccagcacggtgcact 599
|||||||||||||||||||
Sbjct: 382 aggtccagcacggtgcact 364
>gb|BQ658222.1|BQ658222 HA05D15u HA Hordeum vulgare subsp. vulgare cDNA clone HA05D15
3-PRIME, mRNA sequence
Length = 509
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Plus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 412 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 456
>gb|BQ659524.1|BQ659524 HE01L03u HE Hordeum vulgare subsp. vulgare cDNA clone HE01L03
3-PRIME, mRNA sequence
Length = 533
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Plus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 487 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 531
>gb|BU974771.1|BU974771 HB29A24r BC Hordeum vulgare subsp. vulgare cDNA clone HB29A24
5-PRIME, mRNA sequence
Length = 667
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Minus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 278 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 234
>gb|CB877071.1|CB877071 HP03H10T HP Hordeum vulgare subsp. vulgare cDNA clone HP03H10
5-PRIME, mRNA sequence
Length = 587
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Minus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 223 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 179
>gb|CB879734.1|CB879734 HP03H10w HP Hordeum vulgare subsp. vulgare cDNA clone HP03H10
3-PRIME, mRNA sequence
Length = 628
Score = 42.1 bits (21), Expect = 0.025
Identities = 39/45 (86%)
Strand = Plus / Plus
Query: 481 cttgaggaagacagcattcgctggcggaatggcctcaaacatgtc 525
|||||| | |||||||| ||| |||||||||| |||||||||||
Sbjct: 413 cttgagcaggacagcatccgccggcggaatggattcaaacatgtc 457
>gb|BM817346.1|BM817346 HC111F01_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC111F01_T3.ab1 similar to Hexaprenyldihydroxybenzoate
methyltransferase,Apigenin
4'-O-methyltransferase,[Myelin basic protein]-arginine
N-methyltransferase,Catechol
O-methyltransferase,Glycoprotein-fucosylgalactoside
alpha-galactosyltransferase,, mRNA sequence
Length = 905
Score = 40.1 bits (20), Expect = 0.098
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 580 aaggtccagcacggtgcact 599
||||||||||||||||||||
Sbjct: 362 aaggtccagcacggtgcact 343
>gb|AV836790.1|AV836790 AV836790 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd3c22, mRNA
sequence
Length = 696
Score = 38.2 bits (19), Expect = 0.39
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 581 aggtccagcacggtgcact 599
|||||||||||||||||||
Sbjct: 487 aggtccagcacggtgcact 505
>gb|BQ134698.1|BQ134698 LP20027 Lemma/palea-enriched cDNA library from gelatinous stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 2-27
similar to Hexaprenyldihydroxybenzoate
methyltransferase,(S)-scoulerine 9-O- methyltransferase,
mRNA sequence
Length = 299
Score = 38.2 bits (19), Expect = 0.39
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 581 aggtccagcacggtgcact 599
|||||||||||||||||||
Sbjct: 229 aggtccagcacggtgcact 211
>gb|BQ294531.1|BQ294531 LP10296 Lemma/palea-enriched cDNA library from elongation stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 1-296,
mRNA sequence
Length = 293
Score = 38.2 bits (19), Expect = 0.39
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 581 aggtccagcacggtgcact 599
|||||||||||||||||||
Sbjct: 71 aggtccagcacggtgcact 89
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 71,983
Number of Sequences: 312970
Number of extensions: 71983
Number of successful extensions: 22495
Number of sequences better than 0.5: 23
Number of HSP's better than 0.5 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 22442
Number of HSP's gapped (non-prelim): 51
length of query: 724
length of database: 175,134,539
effective HSP length: 19
effective length of query: 705
effective length of database: 169,188,109
effective search space: 119277616845
effective search space used: 119277616845
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)