BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3024030.2.1
(412 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ661861.1|BQ661861 HP01J03w HP Hordeum vulgare subsp. v... 48 2e-004
gb|CD662436.1|CD662436 UCRHV18_01cb02_b1 Drought-stressed D... 48 2e-004
gb|BQ468330.1|BQ468330 HP01P03T HP Hordeum vulgare subsp. v... 42 0.014
gb|BQ661945.1|BQ661945 HP01P03w HP Hordeum vulgare subsp. v... 42 0.014
gb|BU990250.1|BU990250 HF24I08r HF Hordeum vulgare subsp. v... 42 0.014
gb|CB878714.1|CB878714 HP09I22T HP Hordeum vulgare subsp. v... 42 0.014
gb|CX662319.1|CX662319 EST 92 Expressed sequences from Barl... 42 0.014
gb|BE422370.1|BE422370 HWM023cG.06r ITEC HWM Barley Leaf Li... 40 0.055
gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence 40 0.055
gb|AV836843.1|AV836843 AV836843 K. Sato unpublished cDNA li... 38 0.22
gb|BF616801.2|BF616801 HVSMEc0012M14f Hordeum vulgare seedl... 38 0.22
gb|BG309371.1|BG309371 HVSMEc0009E09f Hordeum vulgare seedl... 38 0.22
gb|BI954723.1|BI954723 HVSMEm0019H08f Hordeum vulgare green... 38 0.22
gb|BJ468848.1|BJ468848 BJ468848 K. Sato unpublished cDNA li... 38 0.22
gb|BU973494.1|BU973494 HB25C04r BC Hordeum vulgare subsp. v... 38 0.22
>gb|BQ661861.1|BQ661861 HP01J03w HP Hordeum vulgare subsp. vulgare cDNA clone HP01J03
3-PRIME, mRNA sequence
Length = 366
Score = 48.1 bits (24), Expect = 2e-004
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 15 caaaccaaaccaaaccaaaccaaa 38
||||||||||||||||||||||||
Sbjct: 199 caaaccaaaccaaaccaaaccaaa 222
>gb|CD662436.1|CD662436 UCRHV18_01cb02_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_01cb02, mRNA sequence
Length = 456
Score = 48.1 bits (24), Expect = 2e-004
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 15 caaaccaaaccaaaccaaaccaaa 38
||||||||||||||||||||||||
Sbjct: 216 caaaccaaaccaaaccaaaccaaa 239
>gb|BQ468330.1|BQ468330 HP01P03T HP Hordeum vulgare subsp. vulgare cDNA clone HP01P03
5-PRIME, mRNA sequence
Length = 566
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 15 caaaccaaaccaaaccaaacc 35
|||||||||||||||||||||
Sbjct: 425 caaaccaaaccaaaccaaacc 405
Score = 40.1 bits (20), Expect = 0.055
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 19 ccaaaccaaaccaaaccaaa 38
||||||||||||||||||||
Sbjct: 426 ccaaaccaaaccaaaccaaa 407
>gb|BQ661945.1|BQ661945 HP01P03w HP Hordeum vulgare subsp. vulgare cDNA clone HP01P03
3-PRIME, mRNA sequence
Length = 533
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 15 caaaccaaaccaaaccaaacc 35
|||||||||||||||||||||
Sbjct: 142 caaaccaaaccaaaccaaacc 162
Score = 40.1 bits (20), Expect = 0.055
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 19 ccaaaccaaaccaaaccaaa 38
||||||||||||||||||||
Sbjct: 141 ccaaaccaaaccaaaccaaa 160
>gb|BU990250.1|BU990250 HF24I08r HF Hordeum vulgare subsp. vulgare cDNA clone HF24I08
5-PRIME, mRNA sequence
Length = 620
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 392 atgaaggacagcctcgtgccg 412
|||||||||||||||||||||
Sbjct: 21 atgaaggacagcctcgtgccg 1
>gb|CB878714.1|CB878714 HP09I22T HP Hordeum vulgare subsp. vulgare cDNA clone HP09I22
5-PRIME, mRNA sequence
Length = 602
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 15 caaaccaaaccaaaccaaacc 35
|||||||||||||||||||||
Sbjct: 335 caaaccaaaccaaaccaaacc 315
Score = 40.1 bits (20), Expect = 0.055
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 19 ccaaaccaaaccaaaccaaa 38
||||||||||||||||||||
Sbjct: 336 ccaaaccaaaccaaaccaaa 317
>gb|CX662319.1|CX662319 EST 92 Expressed sequences from Barley Hordeum vulgare subsp.
vulgare cDNA clone P92 5', mRNA sequence
Length = 409
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 15 caaaccaaaccaaaccaaacc 35
|||||||||||||||||||||
Sbjct: 270 caaaccaaaccaaaccaaacc 250
Score = 40.1 bits (20), Expect = 0.055
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 19 ccaaaccaaaccaaaccaaa 38
||||||||||||||||||||
Sbjct: 271 ccaaaccaaaccaaaccaaa 252
>gb|BE422370.1|BE422370 HWM023cG.06r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM023cG.06, mRNA sequence
Length = 1120
Score = 40.1 bits (20), Expect = 0.055
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 21 aaaccaaaccaaaccaaagaactcacta 48
||||||||||||||||| || |||||||
Sbjct: 397 aaaccaaaccaaaccaacgatctcacta 370
>gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence
Length = 261265
Score = 40.1 bits (20), Expect = 0.055
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 15 caaaccaaaccaaaccaaaccaaa 38
||||||||||| ||||||||||||
Sbjct: 21540 caaaccaaacccaaccaaaccaaa 21563
Score = 38.2 bits (19), Expect = 0.22
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 15 caaaccaaaccaaaccaaaccaa 37
|||||| ||||||||||||||||
Sbjct: 21545 caaacccaaccaaaccaaaccaa 21567
>gb|AV836843.1|AV836843 AV836843 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd27i08, mRNA
sequence
Length = 370
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 185 agcagcaagtagagaagaa 203
|||||||||||||||||||
Sbjct: 293 agcagcaagtagagaagaa 311
>gb|BF616801.2|BF616801 HVSMEc0012M14f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0012M14f, mRNA sequence
Length = 758
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 19 ccaaaccaaaccaaaccaa 37
|||||||||||||||||||
Sbjct: 6 ccaaaccaaaccaaaccaa 24
>gb|BG309371.1|BG309371 HVSMEc0009E09f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0009E09f, mRNA sequence
Length = 809
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 19 ccaaaccaaaccaaaccaa 37
|||||||||||||||||||
Sbjct: 6 ccaaaccaaaccaaaccaa 24
>gb|BI954723.1|BI954723 HVSMEm0019H08f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0019H08f, mRNA sequence
Length = 644
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 19 ccaaaccaaaccaaaccaa 37
|||||||||||||||||||
Sbjct: 59 ccaaaccaaaccaaaccaa 77
>gb|BJ468848.1|BJ468848 BJ468848 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd27i08 5', mRNA sequence
Length = 324
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 185 agcagcaagtagagaagaa 203
|||||||||||||||||||
Sbjct: 77 agcagcaagtagagaagaa 59
>gb|BU973494.1|BU973494 HB25C04r BC Hordeum vulgare subsp. vulgare cDNA clone HB25C04
5-PRIME, mRNA sequence
Length = 486
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 394 gaaggacagcctcgtgccg 412
|||||||||||||||||||
Sbjct: 19 gaaggacagcctcgtgccg 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 63,431
Number of Sequences: 312970
Number of extensions: 63431
Number of successful extensions: 15892
Number of sequences better than 0.5: 15
Number of HSP's better than 0.5 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15830
Number of HSP's gapped (non-prelim): 41
length of query: 412
length of database: 175,134,539
effective HSP length: 18
effective length of query: 394
effective length of database: 169,501,079
effective search space: 66783425126
effective search space used: 66783425126
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)