BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023663.2.1
(613 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI949868.1|BI949868 HVSMEl0016J08f Hordeum vulgare spike... 168 1e-040
gb|BI952496.1|BI952496 HVSMEm0006K21f Hordeum vulgare green... 40 0.083
>gb|BI949868.1|BI949868 HVSMEl0016J08f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0016J08f, mRNA sequence
Length = 527
Score = 168 bits (85), Expect = 1e-040
Identities = 205/245 (83%)
Strand = Plus / Minus
Query: 352 aacatctttgtcagtagtattatgcaatggtttctgagagttcggttcaccaaacatgac 411
|||||| ||||||| |||||| ||||||| ||||||| ||||||||| |||||||||||
Sbjct: 296 aacatccttgtcagcagtattttgcaatgacttctgagtgttcggttccccaaacatgac 237
Query: 412 agggctcctagttttcctgcgctttgtgattcttccatttgctgtctcaacttcgtattc 471
|| ||| |||||||| |||||||||||||| |||||||||||||||| ||||| |||||
Sbjct: 236 agtactcttagttttcttgcgctttgtgatttttccatttgctgtctccacttcatattc 177
Query: 472 agggtgggttacctttctatgtttaggaatatcctgagacactttctctacctttctcaa 531
| |||| ||||||||||| || ||||||| ||||||| ||||||||||||| ||||
Sbjct: 176 atggtgtgttacctttctgtgattaggaacatcctgacttgctttctctaccttcctcag 117
Query: 532 catgttcgaaattggtgattgtaaaatcccaggtaagtttacgtccttccgattggtctc 591
| ||| |||||||| || |||| || |||| || |||| | | |||||| ||||||||
Sbjct: 116 tacgttagaaattggcgagtgtataactccagatatgtttgcattcttccgtttggtctc 57
Query: 592 cggac 596
||||
Sbjct: 56 aggac 52
Score = 89.7 bits (45), Expect = 1e-016
Identities = 87/101 (86%)
Strand = Plus / Minus
Query: 229 cgtgagcttctagtcaaaagcataagggtcgtcagcatacggattcaaggaaccctcaga 288
||||||||||||| | || ||| ||||||| |||||||| ||||||||||| ||||| ||
Sbjct: 419 cgtgagcttctagcccaatgcaaaagggtcatcagcatatggattcaaggagccctcgga 360
Query: 289 gaataattcaccaatgtttgcaggatgtgcacgggatcttg 329
|||||||| || || ||||||||||| | |||||||||||
Sbjct: 359 aaataattctccgatatttgcaggatggggacgggatcttg 319
>gb|BI952496.1|BI952496 HVSMEm0006K21f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0006K21f, mRNA sequence
Length = 884
Score = 40.1 bits (20), Expect = 0.083
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 268 cggattcaaggaaccctcag 287
||||||||||||||||||||
Sbjct: 394 cggattcaaggaaccctcag 413
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 34,978
Number of Sequences: 312970
Number of extensions: 34978
Number of successful extensions: 8773
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8769
Number of HSP's gapped (non-prelim): 3
length of query: 613
length of database: 175,134,539
effective HSP length: 19
effective length of query: 594
effective length of database: 169,188,109
effective search space: 100497736746
effective search space used: 100497736746
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)