BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950290.2.1
(1442 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. v... 458 e-127
gb|BU985441.1|BU985441 HF07D15r HF Hordeum vulgare subsp. v... 272 3e-071
gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green... 266 2e-069
gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. v... 246 2e-063
gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subs... 188 3e-046
>gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
5-PRIME, mRNA sequence
Length = 654
Score = 458 bits (231), Expect = e-127
Identities = 530/627 (84%), Gaps = 2/627 (0%)
Strand = Plus / Plus
Query: 186 ctcaaggttgc-ttaccagggttgtgctggagcgtacagcgaagcagcagcaaagaaggc 244
||||||||||| ||||||||| | |||||||| |||| || || ||||||||||||||
Sbjct: 21 ctcaaggttgccttaccagggcttccctggagcg-acagtgaggctgcagcaaagaaggc 79
Query: 245 gtacccgaattgtgagactgtgccctgtgagcacttcgacactgcatttcaggctgttca 304
|| || ||||| |||| |||||||| |||||||| || ||||| ||||||||||||||
Sbjct: 80 ctatccaaattgccagacagtgccctgcgagcactttgatactgcgtttcaggctgttca 139
Query: 305 aaactgggtagctgatagggccgtactgccacttgagaattcattgggaggtagcataca 364
||||||| ||| ||||||||| |||||||||||||||||| | ||||| ||||| |||||
Sbjct: 140 aaactggatagttgatagggcagtactgccacttgagaatactttgggcggtagtataca 199
Query: 365 taggaattatgatcttttgcttcggcatagtttgcacattgtaggagaagttcgccttgc 424
| |||| ||||||||||| ||||||||| ||||||| || ||||| |||||||||||
Sbjct: 200 tcggaactatgatcttttacttcggcatgagctgcacatagttggagaggttcgccttgc 259
Query: 425 ggttcgccattgcttattagccaatcctggcgtgaagattgaaaacctgaaaagtgctat 484
||||| |||||||| |||||||||| ||| |||||||| ||||| || | || || ||
Sbjct: 260 agttcggcattgcttgttagccaatcgtggagtgaagatcgaaaatctaagaaatgtcat 319
Query: 485 gagtcatccccaggctcttgcgcaatgtgagcacacattaacatcactaggaattgagca 544
|||||||||||||| ||||||||||||||||| ||| | ||| | |||||||| ||
Sbjct: 320 cagtcatccccaggcccttgcgcaatgtgagcatacactgacagagttgggaattgaaca 379
Query: 545 tagagaagctgttgacgacactgctggtgcagcaaagattgttgcagaacatatgctcca 604
|||| |||||||||| ||||||||||||||||||||| | ||||||||||| ||||||||
Sbjct: 380 tagacaagctgttgatgacactgctggtgcagcaaagttagttgcagaacaaatgctcca 439
Query: 605 ggacactggtgctattgctagttcgttggcggctaaactgtatggattggatgttcttgc 664
|| ||||||||| |||| ||||| || || ||| |||| |||| | ||||| |||||||
Sbjct: 440 agatactggtgctgttgccagttcattagctgctgaactttatgaactggatattcttgc 499
Query: 665 tgagaatattcaggatgacaaagataatgtcacccgttttatgatgctagctcgagaacc 724
||| ||||||||||| || |||| ||||||||||| ||||||||||| ||||| |||||
Sbjct: 500 tgaaaatattcaggacgaaaaagtcaatgtcacccgctttatgatgcttgctcgggaacc 559
Query: 725 catcatacctcgcactgataaaccatttaagacaagcatagtcttttcactagaagaagg 784
|| |||||||| |||||||||||| ||||| ||||| || |||||||| ||||||||
Sbjct: 560 tattatacctcgggttgataaaccattcaagactagcattgtgttttcacttgaagaagg 619
Query: 785 acctggacagctctttaaggcacttgc 811
||| ||||| ||||| |||||||||||
Sbjct: 620 acccggacaactcttcaaggcacttgc 646
>gb|BU985441.1|BU985441 HF07D15r HF Hordeum vulgare subsp. vulgare cDNA clone HF07D15
5-PRIME, mRNA sequence
Length = 411
Score = 272 bits (137), Expect = 3e-071
Identities = 326/389 (83%)
Strand = Plus / Plus
Query: 348 ttgggaggtagcatacataggaattatgatcttttgcttcggcatagtttgcacattgta 407
||||| ||||| |||||| |||| ||||||||||| ||||||||| ||||||| ||
Sbjct: 16 ttgggcggtagtatacatcggaactatgatcttttacttcggcatgagctgcacatagtt 75
Query: 408 ggagaagttcgccttgcggttcgccattgcttattagccaatcctggcgtgaagattgaa 467
||||| ||||||||||| ||||| |||||||| |||||||||| ||| |||||||| |||
Sbjct: 76 ggagaggttcgccttgcagttcggcattgcttgttagccaatcgtggagtgaagatcgaa 135
Query: 468 aacctgaaaagtgctatgagtcatccccaggctcttgcgcaatgtgagcacacattaaca 527
|| || | || || || |||||||||||||| ||||||||||||||||| ||| | |||
Sbjct: 136 aatctaagaaatgtcatcagtcatccccaggcccttgcgcaatgtgagcatacactgaca 195
Query: 528 tcactaggaattgagcatagagaagctgttgacgacactgctggtgcagcaaagattgtt 587
| |||||||| |||||| |||||||||| ||||||||||||||||||||| | |||
Sbjct: 196 gagttgggaattgaacatagacaagctgttgatgacactgctggtgcagcaaagttagtt 255
Query: 588 gcagaacatatgctccaggacactggtgctattgctagttcgttggcggctaaactgtat 647
|||||||| |||||||| || ||||||||| |||| ||||| || || ||| |||| |||
Sbjct: 256 gcagaacaaatgctccaagatactggtgctgttgccagttcattagctgctgaactttat 315
Query: 648 ggattggatgttcttgctgagaatattcaggatgacaaagataatgtcacccgttttatg 707
| | ||||| |||||||||| ||||||||||| || |||| |||| |||||| ||||||
Sbjct: 316 gaactggatattcttgctgaaaatattcaggacgaaaaagtcaatgccacccgctttatg 375
Query: 708 atgctagctcgagaacccatcatacctcg 736
||||| ||||| ||||| || ||||||||
Sbjct: 376 atgcttgctcgggaacctattatacctcg 404
>gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0011K04f, mRNA sequence
Length = 636
Score = 266 bits (134), Expect = 2e-069
Identities = 464/574 (80%)
Strand = Plus / Plus
Query: 219 tacagcgaagcagcagcaaagaaggcgtacccgaattgtgagactgtgccctgtgagcac 278
|||||||| || || || |||||||| ||||||| || ||||| |||||||| ||| ||
Sbjct: 7 tacagcgaggccgccgccaagaaggcctacccgagctgcgagaccgtgccctgcgagtac 66
Query: 279 ttcgacactgcatttcaggctgttcaaaactgggtagctgatagggccgtactgccactt 338
||||| || || || ||||||||| |||| ||||| |||||| | ||||| |||||||
Sbjct: 67 ttcgagaccgccttccaggctgttgaaaattgggtggctgatcgcgccgtgttgccacta 126
Query: 339 gagaattcattgggaggtagcatacataggaattatgatcttttgcttcggcatagtttg 398
||||| |||||||| |||||||||||| | || ||||| || | || || ||| | |||
Sbjct: 127 gagaactcattgggtggtagcatacatcgcaactatgacctcctactacgccatcggttg 186
Query: 399 cacattgtaggagaagttcgccttgcggttcgccattgcttattagccaatcctggcgtg 458
|||||||| || || || || || |||||||| |||||||| | || |||| ||| ||
Sbjct: 187 cacattgttggcgaggtacggctcgcggttcggcattgcttgctggcgaatcgtggtgtc 246
Query: 459 aagattgaaaacctgaaaagtgctatgagtcatccccaggctcttgcgcaatgtgagcac 518
|||||||| |||||| ||||| ||||| |||||||| |||||||| || ||||||||
Sbjct: 247 aagattgagaacctgcggagtgccatgagccatccccaagctcttgcacagtgtgagcaa 306
Query: 519 acattaacatcactaggaattgagcatagagaagctgttgacgacactgctggtgcagca 578
||| | ||| ||||||||||| ||||||||||||||||| ||||| || || |||||
Sbjct: 307 acactgacaaagctaggaattgaacatagagaagctgttgatgacacagccggcgcagcc 366
Query: 579 aagattgttgcagaacatatgctccaggacactggtgctattgctagttcgttggcggct 638
||| |||||||||| | ||||| ||||| | ||| |||| |||||| |||| |||
Sbjct: 367 aagcacattgcagaacaaaatctccaagacaccgctgcagttgccagttcggtggctgct 426
Query: 639 aaactgtatggattggatgttcttgctgagaatattcaggatgacaaagataatgtcacc 698
|||| |||||| ||||| ||||||| || ||||| ||||||||| |||||||| |||
Sbjct: 427 caactttatggactggatattcttgcagaaaatatccaggatgacgcggataatgtgacc 486
Query: 699 cgttttatgatgctagctcgagaacccatcatacctcgcactgataaaccatttaagaca 758
|| ||||||||||| ||||| |||||||| || |||||||||||||| ||||| |||||
Sbjct: 487 cgctttatgatgctggctcgggaacccattattcctcgcactgataagccattcaagact 546
Query: 759 agcatagtcttttcactagaagaaggacctggac 792
|||||||| || || || |||||| |||||||||
Sbjct: 547 agcatagtgttctctcttgaagaaagacctggac 580
>gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
3-PRIME, mRNA sequence
Length = 612
Score = 246 bits (124), Expect = 2e-063
Identities = 299/355 (84%), Gaps = 3/355 (0%)
Strand = Plus / Minus
Query: 690 aatgtcacccgttttatgatgctagctcgagaacccatcatacctcgcactgataaacca 749
||||||||||| ||||||||||| ||||| ||||| || |||||||| ||||||||||
Sbjct: 611 aatgtcacccgctttatgatgcttgctcgggaacctattatacctcgggttgataaacca 552
Query: 750 tttaagacaagcatagtcttttcactagaagaaggacctggacagctctttaaggcactt 809
|| ||||| ||||| || |||||||| ||||||||||| ||||| ||||| |||||||||
Sbjct: 551 ttcaagactagcattgtgttttcacttgaagaaggacccggacaactcttcaaggcactt 492
Query: 810 gctgtatttgcactgagggacatcaacctcacaaagattgaaagtcgtccacataaggaa 869
|| || |||||||||||||| ||||||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 491 gcagtttttgcactgagggaaatcaacctcaccaagatcgaaagccgtccacacaagaaa 432
Query: 870 aggcctcttcgggtatctgatg--actgt-tcctcgctcttaaagaactttgattacctt 926
|||||| |||| ||| |||||| ||| | ||| | | | | ||| ||||||| |||||
Sbjct: 431 aggccttttcgtgtagctgatgatactttctccacacgcgtcaagtactttgactacctg 372
Query: 927 ttttatgttgaccttgaagcatcaatggctgatccaaagactcagaatgctcttggaaat 986
|||||||| |||||||| || || |||||||||||||| |||||||| ||||| |||||
Sbjct: 371 ttttatgtggaccttgaggcgtctatggctgatccaaaaactcagaacgctctcagaaat 312
Query: 987 ctgaaggagtttgcaacatttttaagagttcttggaagctatcctacagatgtga 1041
|| ||||||| ||||| |||| |||||| || || |||||||||||||| ||||
Sbjct: 311 ttggaggagttcgcaaccttttcaagagtcctagggagctatcctacagacgtga 257
>gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200021A01F1, mRNA sequence
Length = 360
Score = 188 bits (95), Expect = 3e-046
Identities = 240/286 (83%), Gaps = 3/286 (1%)
Strand = Plus / Plus
Query: 759 agcatagtcttttcactagaagaaggacctggacagctctttaaggcacttgctgtattt 818
||||| || |||||||| ||||||||||| ||||| ||||| ||||||||||| || |||
Sbjct: 3 agcattgtgttttcacttgaagaaggacccggacaactcttcaaggcacttgcagttttt 62
Query: 819 gcactgagggacatcaacctcacaaagattgaaagtcgtccacataaggaaaggcctctt 878
||||||||||| ||||||||||| ||||| ||||| |||||||| ||| |||||||| ||
Sbjct: 63 gcactgagggaaatcaacctcaccaagatcgaaagccgtccacacaagaaaaggcctttt 122
Query: 879 cgggtatctgatg--actgt-tcctcgctcttaaagaactttgattaccttttttatgtt 935
|| ||| |||||| ||| | ||| | | | | ||| ||||||| ||||| ||||||||
Sbjct: 123 cgtgtagctgatgatactttctccacacgcgtcaagtactttgactacctgttttatgtg 182
Query: 936 gaccttgaagcatcaatggctgatccaaagactcagaatgctcttggaaatctgaaggag 995
|||||||| || || |||||||||||||| |||||||| ||||| ||||| || |||||
Sbjct: 183 gaccttgaggcgtctatggctgatccaaaaactcagaacgctctcagaaatttggaggag 242
Query: 996 tttgcaacatttttaagagttcttggaagctatcctacagatgtga 1041
|| ||||| |||| |||||| || || |||||||||||||| ||||
Sbjct: 243 ttcgcaaccttttcaagagtcctagggagctatcctacagacgtga 288
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 133,970
Number of Sequences: 312970
Number of extensions: 133970
Number of successful extensions: 35185
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 35170
Number of HSP's gapped (non-prelim): 9
length of query: 1442
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1423
effective length of database: 169,188,109
effective search space: 240754679107
effective search space used: 240754679107
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)