BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950080.2.1
(687 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL504465.1|AL504465 AL504465 Hordeum vulgare Barke roots... 430 e-119
gb|BI957056.1|BI957056 HVSMEn0007D01f Hordeum vulgare rachi... 204 3e-051
gb|BI956049.1|BI956049 HVSMEm0025H22f Hordeum vulgare green... 38 0.37
>gb|AL504465.1|AL504465 AL504465 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05F23V 5', mRNA sequence
Length = 579
Score = 430 bits (217), Expect = e-119
Identities = 393/453 (86%)
Strand = Plus / Plus
Query: 37 cgcgcgtccgaacgtgctagtgacggggacgccggggacggggaagacgaccacgtgctc 96
|||||| || |||||||| |||| |||||||||||||||||||||||||| || |||||
Sbjct: 68 cgcgcggcccaacgtgctgatgaccgggacgccggggacggggaagacgacgacntgctc 127
Query: 97 gctccttgccgacgccgcaggcctccgccacgtcaacatcggcgacctagtccgcgagaa 156
||||| |||||||| || ||| | ||||||||||||||||||||| |||||||||||
Sbjct: 128 cctcctcgccgacgcggccggcgtggcccacgtcaacatcggcgacctcgtccgcgagaa 187
Query: 157 gagccttcacgacggctgggacgccgacctcgagtgccatgtcatcaacgaggacctggt 216
| | || |||||||||||||||| |||||||| ||||| ||||||||||||||||||||
Sbjct: 188 ggggctccacgacggctgggacgaggacctcgactgccacgtcatcaacgaggacctggt 247
Query: 217 gtgcgacgagcttgaggacatgatggaagaaggtggtatactagtagattaccacggatg 276
|| || ||||| ||||||| |||||||||||| ||| |||| |||||||| ||||||||
Sbjct: 248 ttgtgatgagctcgaggacaggatggaagaaggcggtgtactggtagattatcacggatg 307
Query: 277 tgatttctttccagaacgttggtttgacctcgtagttgtgctccagactgacaactcaat 336
||||||||||||||| |||||||||||||| || | | ||| ||||| |||||||||||
Sbjct: 308 tgatttctttccagagcgttggtttgaccttgtggncgngcttcagaccgacaactcaat 367
Query: 337 tctgcatgatcgcctgacgggcagaggttacatgggttccaagctcgccaacaacataga 396
||||||||||||| |||| ||||||| ||||||||| |||||||| | |||||||||||
Sbjct: 368 tctgcatgatcgcttgactagcagaggctacatgggtgccaagctcacaaacaacataga 427
Query: 397 gtgcgaaatcttccaagtgctcctcgaggaggcgagggagagctacagagaggacatcgt 456
||| || |||||||| |||||||| |||||||||||||||||||||| ||||| || ||
Sbjct: 428 gtgtgagatcttccaggtgctcctagaggaggcgagggagagctacaaggaggatatagt 487
Query: 457 gatgccattgcgaagtgacaacgtggaggatat 489
|||||| |||||||||||| ||| |||||||
Sbjct: 488 gatgccgctgcgaagtgacagcgtcnaggatat 520
>gb|BI957056.1|BI957056 HVSMEn0007D01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007D01f, mRNA sequence
Length = 626
Score = 204 bits (103), Expect = 3e-051
Identities = 212/247 (85%), Gaps = 1/247 (0%)
Strand = Plus / Minus
Query: 243 aagaaggtggtatactagtagattaccacggatgtgatttctttccagaacgttggtttg 302
||||||| ||| || | |||||||| |||||||||||||||| |||||| ||||||||||
Sbjct: 626 aagaaggcggtgtattggtagattatcacggatgtgatttctctccagagcgttggtttg 567
Query: 303 acctcgtagttgtgctccagactgacaactcaattctgcatgatcgcctgacgggcagag 362
|||| || || ||||| ||||| ||||||||||| ||||||||||| |||| ||||||
Sbjct: 566 accttgtggtcgtgcttcagacc-acaactcaattttgcatgatcgcttgactagcagag 508
Query: 363 gttacatgggttccaagctcgccaacaacatagagtgcgaaatcttccaagtgctcctcg 422
| ||||||||| |||||||| | |||||||||||||| || |||||||| |||||||| |
Sbjct: 507 gctacatgggtgccaagctcacaaacaacatagagtgtgagatcttccaggtgctcctag 448
Query: 423 aggaggcgagggagagctacagagaggacatcgtgatgccattgcgaagtgacaacgtgg 482
|| |||||||||||||||||| ||||| || ||||||| |||||||||||| ||| |
Sbjct: 447 agaaggcgagggagagctacaaggaggatatagtgatgctgctgcgaagtgacagcgtcg 388
Query: 483 aggatat 489
|||||||
Sbjct: 387 aggatat 381
>gb|BI956049.1|BI956049 HVSMEm0025H22f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025H22f, mRNA sequence
Length = 670
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 61 ggggacgccggggacgggg 79
|||||||||||||||||||
Sbjct: 615 ggggacgccggggacgggg 633
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 110,721
Number of Sequences: 312970
Number of extensions: 110721
Number of successful extensions: 28656
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 28651
Number of HSP's gapped (non-prelim): 3
length of query: 687
length of database: 175,134,539
effective HSP length: 19
effective length of query: 668
effective length of database: 169,188,109
effective search space: 113017656812
effective search space used: 113017656812
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)