BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921788.2.1
(606 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG299831.1|BG299831 HVSMEa0022A04f Hordeum vulgare seedl... 153 8e-036
gb|BQ768374.1|BQ768374 EBro08_SQ011_D16 _R root, 3 week, dr... 153 8e-036
gb|CA012582.1|CA012582 HT05N12r HT Hordeum vulgare subsp. v... 153 8e-036
gb|BQ759924.1|BQ759924 EBpi05_SQ004_I23_R pistil, 8 DPA, no... 100 1e-019
gb|AJ463443.1|AJ463443 AJ463443 S00002 Hordeum vulgare subs... 88 4e-016
gb|BU996191.1|BU996191 HM12M10r HM Hordeum vulgare subsp. v... 86 2e-015
gb|BI958315.1|BI958315 HVSMEn0014G02f Hordeum vulgare rachi... 42 0.021
gb|CA027717.1|CA027717 HZ59M20r HZ Hordeum vulgare subsp. v... 38 0.32
>gb|BG299831.1|BG299831 HVSMEa0022A04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0022A04f, mRNA sequence
Length = 541
Score = 153 bits (77), Expect = 8e-036
Identities = 128/145 (88%)
Strand = Plus / Plus
Query: 178 tggtgcttcagcgatccggagatgaaaaggcggcggcgggtggccagctacaaggcttac 237
|||||||||||||| |||||| |||| |||||||| |||||||| ||||||||||| |||
Sbjct: 225 tggtgcttcagcgacccggaggtgaagaggcggcgtcgggtggcgagctacaaggcatac 284
Query: 238 tcggcggagggcaaagtcaaggcatcgttccccaggggattccgctggatcaaggccaag 297
|||| |||||| ||||||||||| ||||| | | ||||||||||||||||||||| ||||
Sbjct: 285 tcggtggagggaaaagtcaaggcctcgtttcgccggggattccgctggatcaaggacaag 344
Query: 298 tgctccgagctcatccatggctgaa 322
||| ||| |||||||||||||||
Sbjct: 345 tgcaccggcttcatccatggctgaa 369
Score = 121 bits (61), Expect = 3e-026
Identities = 105/119 (88%), Gaps = 3/119 (2%)
Strand = Plus / Plus
Query: 1 tacgaccgcgcgcaccgcccctacgcggctcccgcccccgccggtgagtacgaccgcccc 60
|||||||||||| ||||||| |||||| ||| || ||||||| || ||||||||||||
Sbjct: 36 tacgaccgcgcgtaccgcccgtacgcg---ccctcctccgccggcgactacgaccgcccc 92
Query: 61 taccgcaacgaggtcgtgccctacggtgaccgccgcatcgacatcatcgtcaagccgcc 119
|||||||||||| ||||||||||||| ||||||||||||||| || | |||||||||||
Sbjct: 93 taccgcaacgagatcgtgccctacggcgaccgccgcatcgacctcgtggtcaagccgcc 151
>gb|BQ768374.1|BQ768374 EBro08_SQ011_D16 _R root, 3 week, drought-stressed, cv Optic,
EBro08 Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_D16 5', mRNA sequence
Length = 510
Score = 153 bits (77), Expect = 8e-036
Identities = 128/145 (88%)
Strand = Plus / Plus
Query: 178 tggtgcttcagcgatccggagatgaaaaggcggcggcgggtggccagctacaaggcttac 237
|||||||||||||| |||||| |||| |||||||| |||||||| ||||||||||| |||
Sbjct: 47 tggtgcttcagcgacccggaggtgaagaggcggcgtcgggtggcgagctacaaggcatac 106
Query: 238 tcggcggagggcaaagtcaaggcatcgttccccaggggattccgctggatcaaggccaag 297
|||| |||||| ||||||||||| ||||| | | ||||||||||||||||||||| ||||
Sbjct: 107 tcggtggagggaaaagtcaaggcctcgtttcgccggggattccgctggatcaaggacaag 166
Query: 298 tgctccgagctcatccatggctgaa 322
||| ||| |||||||||||||||
Sbjct: 167 tgcaccggcttcatccatggctgaa 191
>gb|CA012582.1|CA012582 HT05N12r HT Hordeum vulgare subsp. vulgare cDNA clone HT05N12
5-PRIME, mRNA sequence
Length = 601
Score = 153 bits (77), Expect = 8e-036
Identities = 128/145 (88%)
Strand = Plus / Plus
Query: 178 tggtgcttcagcgatccggagatgaaaaggcggcggcgggtggccagctacaaggcttac 237
|||||||||||||| |||||| |||| |||||||| |||||||| ||||||||||| |||
Sbjct: 163 tggtgcttcagcgacccggaggtgaagaggcggcgtcgggtggcgagctacaaggcatac 222
Query: 238 tcggcggagggcaaagtcaaggcatcgttccccaggggattccgctggatcaaggccaag 297
|||| |||||| ||||||||||| ||||| | | ||||||||||||||||||||| ||||
Sbjct: 223 tcggtggagggaaaagtcaaggcctcgtttcgccggggattccgctggatcaaggacaag 282
Query: 298 tgctccgagctcatccatggctgaa 322
||| ||| |||||||||||||||
Sbjct: 283 tgcaccggcttcatccatggctgaa 307
Score = 111 bits (56), Expect = 3e-023
Identities = 74/80 (92%)
Strand = Plus / Plus
Query: 40 gccggtgagtacgaccgcccctaccgcaacgaggtcgtgccctacggtgaccgccgcatc 99
||||| || |||||||||||||||||||||||| ||||||||||||| ||||||||||||
Sbjct: 10 gccggcgactacgaccgcccctaccgcaacgagatcgtgccctacggcgaccgccgcatc 69
Query: 100 gacatcatcgtcaagccgcc 119
||| || |||||||||||||
Sbjct: 70 gacctcgtcgtcaagccgcc 89
>gb|BQ759924.1|BQ759924 EBpi05_SQ004_I23_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_I23 5', mRNA sequence
Length = 317
Score = 99.6 bits (50), Expect = 1e-019
Identities = 71/78 (91%)
Strand = Plus / Plus
Query: 177 gtggtgcttcagcgatccggagatgaaaaggcggcggcgggtggccagctacaaggctta 236
||||||||||||||| ||||||||||| |||||||||||||||| ||||||||||||||
Sbjct: 96 gtggtgcttcagcgacccggagatgaagcggcggcggcgggtggcgagctacaaggctta 155
Query: 237 ctcggcggagggcaaagt 254
||| | |||||| |||||
Sbjct: 156 ctcagtggaggggaaagt 173
>gb|AJ463443.1|AJ463443 AJ463443 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200076F03F1, mRNA sequence
Length = 420
Score = 87.7 bits (44), Expect = 4e-016
Identities = 80/92 (86%)
Strand = Plus / Plus
Query: 229 aaggcttactcggcggagggcaaagtcaaggcatcgttccccaggggattccgctggatc 288
||||| ||||||| |||||| ||||||||||| ||||| | | |||||||||||||||||
Sbjct: 1 aaggcatactcggtggagggaaaagtcaaggcctcgtttcgccggggattccgctggatc 60
Query: 289 aaggccaagtgctccgagctcatccatggctg 320
|||| ||||||| ||| |||||||||||||
Sbjct: 61 aaggacaagtgcaccggcttcatccatggctg 92
>gb|BU996191.1|BU996191 HM12M10r HM Hordeum vulgare subsp. vulgare cDNA clone HM12M10
5-PRIME, mRNA sequence
Length = 429
Score = 85.7 bits (43), Expect = 2e-015
Identities = 112/131 (85%), Gaps = 3/131 (2%)
Strand = Plus / Plus
Query: 193 ccggagatgaaaaggcggcggcgggtggcca-gctacaaggcttactcggcggagggcaa 251
|||||| |||| |||||||| |||||||| | ||| |||||| ||||||| |||||| ||
Sbjct: 12 ccggaggtgaagaggcggcgtcgggtggcgaagct-caaggcatactcggtggagggaaa 70
Query: 252 agtcaaggcatcgttccccaggggattccgctggatcaaggccaagtgctccgagctcat 311
||| ||||| ||||| | | ||||||||||||||||||||| ||||||| ||| ||||
Sbjct: 71 agt-aaggcctcgtttcgccggggattccgctggatcaaggacaagtgcaccggcttcat 129
Query: 312 ccatggctgaa 322
|||||||||||
Sbjct: 130 ccatggctgaa 140
>gb|BI958315.1|BI958315 HVSMEn0014G02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0014G02f, mRNA sequence
Length = 667
Score = 42.1 bits (21), Expect = 0.021
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 150 gggcggcgggggaggggtggg 170
|||||||||||||||||||||
Sbjct: 572 gggcggcgggggaggggtggg 592
>gb|CA027717.1|CA027717 HZ59M20r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59M20
5-PRIME, mRNA sequence
Length = 317
Score = 38.2 bits (19), Expect = 0.32
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 387 gatggtttgtctgttgtta 405
|||||||||||||||||||
Sbjct: 218 gatggtttgtctgttgtta 200
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 112,829
Number of Sequences: 312970
Number of extensions: 112829
Number of successful extensions: 27363
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 27344
Number of HSP's gapped (non-prelim): 18
length of query: 606
length of database: 175,134,539
effective HSP length: 19
effective length of query: 587
effective length of database: 169,188,109
effective search space: 99313419983
effective search space used: 99313419983
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)