BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2835489.2.1
(885 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV913828.1|AV913828 AV913828 K. Sato unpublished cDNA li... 315 1e-084
gb|CA006048.1|CA006048 HU05G22u HU Hordeum vulgare subsp. v... 315 1e-084
gb|AV921443.1|AV921443 AV921443 K. Sato unpublished cDNA li... 190 5e-047
gb|BI958165.1|BI958165 HVSMEn0013L05f Hordeum vulgare rachi... 38 0.48
>gb|AV913828.1|AV913828 AV913828 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23h03 5', mRNA sequence
Length = 617
Score = 315 bits (159), Expect = 1e-084
Identities = 395/471 (83%), Gaps = 2/471 (0%)
Strand = Plus / Minus
Query: 396 gagccattaaacggaatactaaggtcagttctatctcctgtccattcggcatatggaacc 455
||||||||||| |||||||||||||| |||||||| || || ||| ||||| ||||||
Sbjct: 594 gagccattaaatggaatactaaggtcggttctatcccccaacccttctgcatagggaacc 535
Query: 456 tctccaacaaccaccaccgcataggaaaaccccccagtttcaatcatagcttcggttggg 515
|| || || || || | ||||||||||| | |||||| ||||||| ||||||
Sbjct: 534 tcacctactacaacaattgcataggaaaagttttcttcttcaatggtagcttctgttggg 475
Query: 516 catttctcatacacaacttcagtttgcactcccactaattctt-gtatggcctccaatat 574
|||| |||||||||||||| |||| |||| || | ||||| |||||||||| |||||
Sbjct: 474 cattcttcatacacaacttccgtttccacttgcaa-agttctttgtatggcctctaatat 416
Query: 575 gctcgtaccaagagttatttttccactgtctccatgccatgctatcgtccagccaccaca 634
||| |||||| ||||||| |||||||||||||| ||||| ||||||||||| ||||||||
Sbjct: 415 gcttgtaccaggagttatctttccactgtctccgtgccacgctatcgtccacccaccaca 356
Query: 635 ttgatatccaatatcatcagcatgtgttcctgcaaccagtattctttttgcatctttggc 694
|||||||||||||| ||||||||||| |||| || |||||||||||||||| ||||||
Sbjct: 355 ttgatatccaatattgtcagcatgtgtccctgtgacaagtattctttttgcatttttggc 296
Query: 695 caatggaaggaatggcttattctgatccttgccatttttcagaagtaccaaagacttgcg 754
|| ||||||||||| | || |||| |||||||||||||||||||||||||||||| ||
Sbjct: 295 taagggaaggaatggttccttttgattcttgccatttttcagaagtaccaaagactttcg 236
Query: 755 aacagcctcacgcgctagtaaccgatgttccttacaaccaactatgtccagtagagattg 814
|||||||||||| ||||| ||||| ||||| ||||||| || ||| ||||||| | |
Sbjct: 235 aacagcctcacgtgctagctgacgatgctccttgcaaccaattacgtcaagtagagttgg 176
Query: 815 gtctgaaaatggatgctcaaacactccagaaataaacttaaccctcagaat 865
|||||||||||||||||||| ||||||||||| |||||||| || |||||
Sbjct: 175 atctgaaaatggatgctcaaaaactccagaaatgaacttaactcttagaat 125
>gb|CA006048.1|CA006048 HU05G22u HU Hordeum vulgare subsp. vulgare cDNA clone HU05G22
3-PRIME, mRNA sequence
Length = 547
Score = 315 bits (159), Expect = 1e-084
Identities = 273/311 (87%)
Strand = Plus / Plus
Query: 111 aacatttttagcccatatccaacagggaatagcgggtcatagttagagtctccagcatct 170
|||||||| | ||||||||||| |||||||| || |||||||||| |||||| ||| |
Sbjct: 237 aacattttcaacccatatccaaaagggaataagggatcatagttagcatctccaacattt 296
Query: 171 ataggcaattgatcaacagacttacaccatgtcacaggcaatgtgcccacaaaatcatga 230
||||||||||||||| ||||| || |||| || ||||| ||||| |||||||||||||||
Sbjct: 297 ataggcaattgatcagcagacctaaaccacgttacaggtaatgtacccacaaaatcatga 356
Query: 231 tctccaaagaggcaatcggtaattcccatgccctcacttccaggcagccaggcagcgact 290
||||||||||||||||||||||||||||||||||||||||| || ||||||||||| |||
Sbjct: 357 tctccaaagaggcaatcggtaattcccatgccctcacttccgggtagccaggcagcaact 416
Query: 291 agagcttctatcttttccaaaacttgtgactcaataattaatggccttccagatataaca 350
||||| ||||||||||||| |||||||| |||||||| || ||||||||||||| ||||
Sbjct: 417 agagcatctatcttttccagaacttgtggctcaataaccaaaggccttccagatacaaca 476
Query: 351 atcactagggtagggattttacttgcaacacggatgataaggtcagagccattaaacgga 410
|||||||| ||||||| |||||| |||||||| | || ||||| || |||||||| ||
Sbjct: 477 atcactagagtagggactttactagcaacacgagtaatcaggtctgaaccattaaatggg 536
Query: 411 atactaaggtc 421
|||||||||||
Sbjct: 537 atactaaggtc 547
>gb|AV921443.1|AV921443 AV921443 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23h03 3', mRNA sequence
Length = 612
Score = 190 bits (96), Expect = 5e-047
Identities = 222/264 (84%)
Strand = Plus / Plus
Query: 167 atctataggcaattgatcaacagacttacaccatgtcacaggcaatgtgcccacaaaatc 226
||||||||||| |||||| | || || |||| || || ||||| ||||| ||||||||
Sbjct: 176 atctataggcagttgatcgtctgatctaaaccaagttactggcaacgtgcctacaaaatc 235
Query: 227 atgatctccaaagaggcaatcggtaattcccatgccctcacttccaggcagccaggcagc 286
||| ||||||||||||||||| || | ||||||||||||||||||||||| ||| ||| |
Sbjct: 236 atggtctccaaagaggcaatcagtcactcccatgccctcacttccaggcaaccaagcaac 295
Query: 287 gactagagcttctatcttttccaaaacttgtgactcaataattaatggccttccagatat 346
|||||||| |||| ||| || | |||||||| |||||||| || || |||||||| ||
Sbjct: 296 aactagagcatctaccttctcaagaacttgtggctcaataaccaagggtcttccagaaat 355
Query: 347 aacaatcactagggtagggattttacttgcaacacggatgataaggtcagagccattaaa 406
|||||||||||||||||||| ||| || |||||||| | || || || |||||||||||
Sbjct: 356 aacaatcactagggtagggacttttctagcaacacgagtaattagatctgagccattaaa 415
Query: 407 cggaatactaaggtcagttctatc 430
|||||||||||||| ||||||||
Sbjct: 416 tggaatactaaggtcggttctatc 439
Score = 54.0 bits (27), Expect = 8e-006
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 557 ttgtatggcctccaatatgctcgtaccaagagttatttttccact 601
|||||||||||| |||||||| |||||| ||||||| ||| ||||
Sbjct: 568 ttgtatggcctctaatatgcttgtaccangagttatctttncact 612
>gb|BI958165.1|BI958165 HVSMEn0013L05f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0013L05f, mRNA sequence
Length = 917
Score = 38.2 bits (19), Expect = 0.48
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 452 aacctctccaacaaccacc 470
|||||||||||||||||||
Sbjct: 208 aacctctccaacaaccacc 226
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 65,474
Number of Sequences: 312970
Number of extensions: 65474
Number of successful extensions: 18194
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18182
Number of HSP's gapped (non-prelim): 11
length of query: 885
length of database: 175,134,539
effective HSP length: 19
effective length of query: 866
effective length of database: 169,188,109
effective search space: 146516902394
effective search space used: 146516902394
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)