BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750763.3.1
(819 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachi... 551 e-155
gb|BI959049.1|BI959049 HVSMEn0017O16f Hordeum vulgare rachi... 192 1e-047
gb|BI960276.1|BI960276 HVSMEn0024B03f Hordeum vulgare rachi... 42 0.028
gb|BG369010.2|BG369010 HVSMEi0022C19f Hordeum vulgare 20 DA... 38 0.44
gb|BU973162.1|BU973162 HB23P07r BC Hordeum vulgare subsp. v... 38 0.44
>gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018A17f, mRNA sequence
Length = 632
Score = 551 bits (278), Expect = e-155
Identities = 393/430 (91%), Gaps = 1/430 (0%)
Strand = Plus / Plus
Query: 391 aaccccatcgacgactgctggcgctgcgactcggactggcacaacaaccggaagcgcctc 450
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 14 aaccccatcgacgactgctggcgctgcgactcggactggcacaacaaccggcagcgcctc 73
Query: 451 gccgactgtggcatcggctttggccgcaacgccatcggcggccgtgacggcaagatctac 510
|||||||| ||||||||||| ||||||||||||||||||||||| |||||||||||||||
Sbjct: 74 gccgactgcggcatcggcttcggccgcaacgccatcggcggccgcgacggcaagatctac 133
Query: 511 gtggtcaccgacccgagcgatgacgaccctgtca-acccgcgcaagggcaccctccgcta 569
|||||||||||| || |||| |||||||| | | |||| ||||||||||| |||||
Sbjct: 134 gtggtcaccgacgcgggcgacgacgacccggggagaccccaagaagggcaccctgcgcta 193
Query: 570 cgccgtcatccacgaagagccgctctggatcatcttcaagcgggacatggtcatcacgct 629
|||||||||||| || |||||||| ||||||||||||||||| |||||||||||||| ||
Sbjct: 194 cgccgtcatccaggacgagccgctgtggatcatcttcaagcgcgacatggtcatcaccct 253
Query: 630 caaggaggagctcatcatgaacagcttcaagaccatcgacggacgcggcgccaacgtgca 689
|| ||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct: 254 cagccaggagctcatcatgaacagcttcaagaccatcgacggccgcggcgccaacgtgca 313
Query: 690 catcgccaatggtgcctgcatcaccatccagtacatcaccaacgtcatcatccacgggct 749
||||||||| || ||||||| ||||||||||||| |||||||||||||||||||||| ||
Sbjct: 314 catcgccaacggcgcctgcaccaccatccagtacgtcaccaacgtcatcatccacggcct 373
Query: 750 ccacatccacgactgcaagcccacggggaacgccatggtgcgcagctcgcccagccacta 809
|||||||||||||||| ||||| || |||||||||||||||||||| |||||||||||
Sbjct: 374 ccacatccacgactgccgccccaccggcaacgccatggtgcgcagctcccccagccacta 433
Query: 810 tgggtggcgc 819
|||||||||
Sbjct: 434 cgggtggcgc 443
>gb|BI959049.1|BI959049 HVSMEn0017O16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0017O16f, mRNA sequence
Length = 684
Score = 192 bits (97), Expect = 1e-047
Identities = 130/141 (92%)
Strand = Plus / Plus
Query: 679 gccaacgtgcacatcgccaatggtgcctgcatcaccatccagtacatcaccaacgtcatc 738
|||||||||||||||||||| || ||||||||||||||||||||| ||||||||||||||
Sbjct: 24 gccaacgtgcacatcgccaacggcgcctgcatcaccatccagtacgtcaccaacgtcatc 83
Query: 739 atccacgggctccacatccacgactgcaagcccacggggaacgccatggtgcgcagctcg 798
|||||||| |||||||||||||||||| ||||| || ||||||||||||||||||||
Sbjct: 84 atccacggcctccacatccacgactgccgccccaccggcaacgccatggtgcgcagctcc 143
Query: 799 cccagccactatgggtggcgc 819
||||||||||| |||||||||
Sbjct: 144 cccagccactacgggtggcgc 164
>gb|BI960276.1|BI960276 HVSMEn0024B03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024B03f, mRNA sequence
Length = 671
Score = 42.1 bits (21), Expect = 0.028
Identities = 33/37 (89%)
Strand = Plus / Plus
Query: 372 cctgtcgtgcgggacaggcaaccccatcgacgactgc 408
|||||||||||| || || ||||||||| ||||||||
Sbjct: 571 cctgtcgtgcggcacggggaaccccatccacgactgc 607
>gb|BG369010.2|BG369010 HVSMEi0022C19f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0022C19f, mRNA sequence
Length = 842
Score = 38.2 bits (19), Expect = 0.44
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 525 gagcgatgacgaccctgtc 543
|||||||||||||||||||
Sbjct: 565 gagcgatgacgaccctgtc 583
>gb|BU973162.1|BU973162 HB23P07r BC Hordeum vulgare subsp. vulgare cDNA clone HB23P07
5-PRIME, mRNA sequence
Length = 513
Score = 38.2 bits (19), Expect = 0.44
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 525 gagcgatgacgaccctgtc 543
|||||||||||||||||||
Sbjct: 198 gagcgatgacgaccctgtc 216
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 84,022
Number of Sequences: 312970
Number of extensions: 84022
Number of successful extensions: 24048
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 24041
Number of HSP's gapped (non-prelim): 7
length of query: 819
length of database: 175,134,539
effective HSP length: 19
effective length of query: 800
effective length of database: 169,188,109
effective search space: 135350487200
effective search space used: 135350487200
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)