BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750698.2.1
(1126 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ753852.1|BQ753852 EBca01_SQ002_A14_R carpel, pre-anthe... 507 e-142
gb|BU988919.1|BU988919 HF19C15r HF Hordeum vulgare subsp. v... 472 e-131
gb|BQ758765.1|BQ758765 EBma07_SQ002_B22_R maternal, 21 DPA,... 125 3e-027
gb|BJ467379.1|BJ467379 BJ467379 K. Sato unpublished cDNA li... 100 2e-019
gb|BG309703.2|BG309703 HVSMEc0012B02f Hordeum vulgare seedl... 90 2e-016
>gb|BQ753852.1|BQ753852 EBca01_SQ002_A14_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_A14 5', mRNA sequence
Length = 631
Score = 507 bits (256), Expect = e-142
Identities = 465/535 (86%)
Strand = Plus / Plus
Query: 8 gagctgctctgcaatctagtgggcagaattgcgctggtgctgaaagattttatgttcacg 67
||||||| |||||||||||||| || ||||| ||||||||||||||||||||||| |||
Sbjct: 92 gagctgcactgcaatctagtggacaaaattgtgctggtgctgaaagattttatgtccaca 151
Query: 68 atgacatctattctgcctttgtttcacagatagtgaagacagtcaaatctataagtgttg 127
| || |||||| || |||||||||| | ||||||||| ||||||| ||| |||||
Sbjct: 152 aggatatctatcctacctttgtttctcgcgtagtgaagatcatcaaatcaatatctgttg 211
Query: 128 gccccccattatcaggcagatatgacatgggtgcaatctgtatgattgagcactctgaga 187
| ||||||||||| |||||||| || |||||||||||||||||||| |||||||||||||
Sbjct: 212 ggcccccattatctggcagatacgatatgggtgcaatctgtatgatagagcactctgaga 271
Query: 188 aacttcagaatcttgttaatgatgctctagacaaaggtgccgaaattgccgtcaggggga 247
||||||||||||||||||||||||||| |||||||||||||||||||| | ||| ||||
Sbjct: 272 gacttcagaatcttgttaatgatgctcttgacaaaggtgccgaaattgctggcagaggga 331
Query: 248 gttttggcaatcttggggaagatgcagttgatcaatttttcccaccaactggcctggtga 307
| ||||| ||| | || |||||||| ||||||||||| || || ||||||| || ||||
Sbjct: 332 gctttgggaatttgggtgaagatgctgttgatcaattctttcccccaactgttctagtga 391
Query: 308 atgttgatcacacaatgaaaattatgcaagaagagacatttggacctattataccaatca 367
||||| |||||||||||||||||||||| |||||| |||| || || ||| |||||||||
Sbjct: 392 atgttaatcacacaatgaaaattatgcaggaagaggcattcgggccgattctaccaatca 451
Query: 368 tgaaattcagttctgatgaagaggctatcaaacttgcaaacgactcaaaatatggtcttg 427
|||||||| |||||||||||||| ||| |||||||||| || || |||||||||||||
Sbjct: 452 tgaaattctcttctgatgaagaggtcatccaacttgcaaatgattctaaatatggtcttg 511
Query: 428 gctgtgctgttttttcgggcaaccagaagcgtgccatcagaatagcgtcccagttacact 487
| |||||||||||||| |||||||||||||||||||||| ||||||||| || |||| |
Sbjct: 512 gttgtgctgttttttctggcaaccagaagcgtgccatcaaaatagcgtctcaactacagt 571
Query: 488 gtggggtagcagcaatcaatgattttgcttcaagttacatgtgccagtctttgcc 542
| || || |||||||||||||||| |||||||||||| ||||| |||||||||||
Sbjct: 572 gcggtgttgcagcaatcaatgattntgcttcaagttatatgtgtcagtctttgcc 626
>gb|BU988919.1|BU988919 HF19C15r HF Hordeum vulgare subsp. vulgare cDNA clone HF19C15
5-PRIME, mRNA sequence
Length = 542
Score = 472 bits (238), Expect = e-131
Identities = 430/494 (87%)
Strand = Plus / Plus
Query: 73 atctattctgcctttgtttcacagatagtgaagacagtcaaatctataagtgttggcccc 132
|||||| || |||||||||| | ||||||||| ||||||| ||| |||||| |||
Sbjct: 28 atctatcctacctttgtttctcgcgtagtgaagatcatcaaatcaatatctgttgggccc 87
Query: 133 ccattatcaggcagatatgacatgggtgcaatctgtatgattgagcactctgagaaactt 192
|||||||| |||||||| || |||||||||||||||||||| ||||||||||||| ||||
Sbjct: 88 ccattatctggcagatacgatatgggtgcaatctgtatgatagagcactctgagagactt 147
Query: 193 cagaatcttgttaatgatgctctagacaaaggtgccgaaattgccgtcagggggagtttt 252
||||||||||||||||||||||| |||||||||||||||||||| | ||| ||||| |||
Sbjct: 148 cagaatcttgttaatgatgctcttgacaaaggtgccgaaattgctggcagagggagcttt 207
Query: 253 ggcaatcttggggaagatgcagttgatcaatttttcccaccaactggcctggtgaatgtt 312
|| ||| | || |||||||| ||||||||||| || || ||||||| || |||||||||
Sbjct: 208 gggaatttgggtgaagatgctgttgatcaattctttcccccaactgttctagtgaatgtt 267
Query: 313 gatcacacaatgaaaattatgcaagaagagacatttggacctattataccaatcatgaaa 372
|||||||||||||||||||||| |||||| |||| || || ||| ||||||||||||||
Sbjct: 268 aatcacacaatgaaaattatgcaggaagaggcattcgggccgattctaccaatcatgaaa 327
Query: 373 ttcagttctgatgaagaggctatcaaacttgcaaacgactcaaaatatggtcttggctgt 432
||| |||||||||||||| ||| |||||||||| || || |||||||||||||| |||
Sbjct: 328 ttctcttctgatgaagaggtcatccaacttgcaaatgattctaaatatggtcttggttgt 387
Query: 433 gctgttttttcgggcaaccagaagcgtgccatcagaatagcgtcccagttacactgtggg 492
||||||||||| |||||||||||||||||||||| ||||||||| || |||| || ||
Sbjct: 388 gctgttttttctggcaaccagaagcgtgccatcaaaatagcgtctcaactacagtgcggt 447
Query: 493 gtagcagcaatcaatgattttgcttcaagttacatgtgccagtctttgccattcggtggt 552
|| ||||||||||||||||||||||||||||| ||||| ||||||||||| || || |||
Sbjct: 448 gttgcagcaatcaatgattttgcttcaagttatatgtgtcagtctttgccctttggcggt 507
Query: 553 gttaaagacagcgg 566
||||||||||||||
Sbjct: 508 gttaaagacagcgg 521
>gb|BQ758765.1|BQ758765 EBma07_SQ002_B22_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ002_B22 5', mRNA sequence
Length = 546
Score = 125 bits (63), Expect = 3e-027
Identities = 171/207 (82%)
Strand = Plus / Plus
Query: 580 gctggcgtagaaggcttgcgagcttgctgccttgtgaagtctgttgtagaagatagattg 639
||||| || ||||||||||| || ||||||||||| ||| | ||||||||||| || | |
Sbjct: 17 gctggtgtggaaggcttgcgggcctgctgccttgtaaaggcagttgtagaagacaggtgg 76
Query: 640 tggccatatattagaacagtgatcccgaagcctatccagtatcccgtctcagagcatgga 699
|||||||| | | |||| ||||||| |||||||| |||||||||||||| ||| ||||
Sbjct: 77 tggccatacgtcaaaacaatgatccccaagcctattcagtatcccgtctcggagaatggg 136
Query: 700 tttgagttccagcagttgcttgtggagactctgtatggctatagcgtgtgggacaggttg 759
|||| ||||||||| |||| || ||||||||||| ||| ||||| ||||||||| ||
Sbjct: 137 tttgcattccagcagctgctcgtcgagactctgtacggcatcagcgtatgggacaggctg 196
Query: 760 cggtcccttgtgaatcttataaagatg 786
| ||| || |||||||| ||||||||
Sbjct: 197 cagtcactcgtgaatctgttaaagatg 223
>gb|BJ467379.1|BJ467379 BJ467379 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26p15 3', mRNA sequence
Length = 455
Score = 99.6 bits (50), Expect = 2e-019
Identities = 146/178 (82%)
Strand = Plus / Minus
Query: 609 ccttgtgaagtctgttgtagaagatagattgtggccatatattagaacagtgatcccgaa 668
|||||| ||| | ||||||||||| || | ||||||||| | | |||| ||||||| ||
Sbjct: 455 ccttgtaaaggcagttgtagaagacaggtggtggccatacgtcaaaacaatgatccccaa 396
Query: 669 gcctatccagtatcccgtctcagagcatggatttgagttccagcagttgcttgtggagac 728
|||||| |||||||||||||| ||| |||| |||| ||||||||| |||| || |||||
Sbjct: 395 gcctattcagtatcccgtctcggagaatgggtttgcattccagcagctgctcgtcgagac 336
Query: 729 tctgtatggctatagcgtgtgggacaggttgcggtcccttgtgaatcttataaagatg 786
|||||| ||| ||||| ||||||||| ||| ||| || |||||||| ||||||||
Sbjct: 335 tctgtacggcatcagcgtatgggacaggctgcagtcactcgtgaatctgttaaagatg 278
>gb|BG309703.2|BG309703 HVSMEc0012B02f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0012B02f, mRNA sequence
Length = 821
Score = 89.7 bits (45), Expect = 2e-016
Identities = 75/85 (88%)
Strand = Plus / Plus
Query: 8 gagctgctctgcaatctagtgggcagaattgcgctggtgctgaaagattttatgttcacg 67
||||||| |||||||||||||| || ||||| ||||||||||||||||||||||| |||
Sbjct: 34 gagctgcactgcaatctagtggacaaaattgtgctggtgctgaaagattttatgtccaca 93
Query: 68 atgacatctattctgcctttgtttc 92
| || |||||| || ||||||||||
Sbjct: 94 aggatatctatcctacctttgtttc 118
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 77,451
Number of Sequences: 312970
Number of extensions: 77451
Number of successful extensions: 21249
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 21243
Number of HSP's gapped (non-prelim): 6
length of query: 1126
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1107
effective length of database: 169,188,109
effective search space: 187291236663
effective search space used: 187291236663
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)