BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2646712.2.1
(581 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU975402.1|BU975402 HB31A09r BC Hordeum vulgare subsp. v... 168 1e-040
gb|BU975696.1|BU975696 HB32A09r BC Hordeum vulgare subsp. v... 168 1e-040
gb|BE230902.1|BE230902 HVSMEg0001E18f Hordeum vulgare pre-a... 161 3e-038
gb|BG344412.1|BG344412 HVSMEg0009D04f Hordeum vulgare pre-a... 92 2e-017
gb|BF254781.2|BF254781 HVSMEf0005A11f Hordeum vulgare seedl... 84 6e-015
gb|BJ450530.1|BJ450530 BJ450530 K. Sato unpublished cDNA li... 80 9e-014
gb|BJ457952.1|BJ457952 BJ457952 K. Sato unpublished cDNA li... 78 4e-013
gb|CA000262.1|CA000262 HS09C23u HS Hordeum vulgare subsp. v... 56 1e-006
gb|CA001150.1|CA001150 HS05E05u HS Hordeum vulgare subsp. v... 56 1e-006
gb|CA010224.1|CA010224 HT11I05u HT Hordeum vulgare subsp. v... 56 1e-006
gb|BI958488.1|BI958488 HVSMEn0015E11f Hordeum vulgare rachi... 54 5e-006
gb|BQ467661.1|BQ467661 HS04H17r HS Hordeum vulgare subsp. v... 48 3e-004
gb|BQ663162.1|BQ663162 HU01A05w HU Hordeum vulgare subsp. v... 48 3e-004
gb|BQ663264.1|BQ663264 HU01M14w HU Hordeum vulgare subsp. v... 48 3e-004
gb|CA000392.1|CA000392 HS08M20u HS Hordeum vulgare subsp. v... 48 3e-004
gb|CA001406.1|CA001406 HS18B01u HS Hordeum vulgare subsp. v... 48 3e-004
gb|CA005674.1|CA005674 HU09D12u HU Hordeum vulgare subsp. v... 48 3e-004
gb|CA006217.1|CA006217 HU05D06u HU Hordeum vulgare subsp. v... 48 3e-004
gb|CA010747.1|CA010747 HT08M24u HT Hordeum vulgare subsp. v... 48 3e-004
gb|CA013550.1|CA013550 HT08M24r HT Hordeum vulgare subsp. v... 48 3e-004
gb|BM444997.2|BM444997 EBem09_SQ007_C03_R embryo, 1 Day ger... 40 0.078
gb|AL504555.1|AL504555 AL504555 Hordeum vulgare Barke roots... 38 0.31
gb|BF616863.3|BF616863 HVSMEc0013C20f Hordeum vulgare seedl... 38 0.31
>gb|BU975402.1|BU975402 HB31A09r BC Hordeum vulgare subsp. vulgare cDNA clone HB31A09
5-PRIME, mRNA sequence
Length = 557
Score = 168 bits (85), Expect = 1e-040
Identities = 181/213 (84%)
Strand = Plus / Minus
Query: 340 gggaggatgcccttctcctggaagcacacgacggtgtcgtacagcgcctgcgccgccggg 399
||||||||||||||||||||||||| |||||||||||||| || ||| ||| ||||
Sbjct: 434 gggaggatgcccttctcctggaagctgacgacggtgtcgtagaggcactgtgccaccggc 375
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggctgcttcctc 459
|||||||| ||||||||||| | ||||||||||| || |||||||||||| ||||||||
Sbjct: 374 gtgaactcgaggcccagctcacggagccgctggttcgacatcttgtacggccgcttcctc 315
Query: 460 gggttcacctcgtcagagcacctctccgggacggggtagcccgggaaggacttgcggagg 519
||||||||| |||||||||||| |||| |||||| | |||||| |||||||||||
Sbjct: 314 gggttcaccgagtcagagcacctgctggggatggggtactcggggaagaacttgcggagg 255
Query: 520 gtgcggacgacgtcctcgcggtggagcacggcg 552
|| ||| |||||||| |||||||||||||||
Sbjct: 254 atggtgaccacgtcctcccggtggagcacggcg 222
>gb|BU975696.1|BU975696 HB32A09r BC Hordeum vulgare subsp. vulgare cDNA clone HB32A09
5-PRIME, mRNA sequence
Length = 559
Score = 168 bits (85), Expect = 1e-040
Identities = 181/213 (84%)
Strand = Plus / Minus
Query: 340 gggaggatgcccttctcctggaagcacacgacggtgtcgtacagcgcctgcgccgccggg 399
||||||||||||||||||||||||| |||||||||||||| || ||| ||| ||||
Sbjct: 434 gggaggatgcccttctcctggaagctgacgacggtgtcgtagaggcactgtgccaccggc 375
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggctgcttcctc 459
|||||||| ||||||||||| | ||||||||||| || |||||||||||| ||||||||
Sbjct: 374 gtgaactcgaggcccagctcacggagccgctggttcgacatcttgtacggccgcttcctc 315
Query: 460 gggttcacctcgtcagagcacctctccgggacggggtagcccgggaaggacttgcggagg 519
||||||||| |||||||||||| |||| |||||| | |||||| |||||||||||
Sbjct: 314 gggttcaccgagtcagagcacctgctggggatggggtactcggggaagaacttgcggagg 255
Query: 520 gtgcggacgacgtcctcgcggtggagcacggcg 552
|| ||| |||||||| |||||||||||||||
Sbjct: 254 atggtgaccacgtcctcccggtggagcacggcg 222
>gb|BE230902.1|BE230902 HVSMEg0001E18f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0001E18f, mRNA sequence
Length = 618
Score = 161 bits (81), Expect = 3e-038
Identities = 180/213 (84%)
Strand = Plus / Minus
Query: 340 gggaggatgcccttctcctggaagcacacgacggtgtcgtacagcgcctgcgccgccggg 399
||||||||||||||||||||||||| |||||||||||||| || ||| ||| ||||
Sbjct: 361 gggaggatgcccttctcctggaagctgacgacggtgtcgtagaggcactgtgccaccggc 302
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggctgcttcctc 459
|||||||| ||||||||||| | |||||| |||| || |||||||||||| ||||||||
Sbjct: 301 gtgaactcgaggcccagctcacggagccgccggttcgacatcttgtacggccgcttcctc 242
Query: 460 gggttcacctcgtcagagcacctctccgggacggggtagcccgggaaggacttgcggagg 519
||||||||| |||||||||||| |||| |||||| | |||||| |||||||||||
Sbjct: 241 gggttcaccgagtcagagcacctgctggggatggggtactcggggaagaacttgcggagg 182
Query: 520 gtgcggacgacgtcctcgcggtggagcacggcg 552
|| ||| |||||||| |||||||||||||||
Sbjct: 181 atggtgaccacgtcctcccggtggagcacggcg 149
>gb|BG344412.1|BG344412 HVSMEg0009D04f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009D04f, mRNA sequence
Length = 850
Score = 91.7 bits (46), Expect = 2e-017
Identities = 121/146 (82%)
Strand = Plus / Plus
Query: 429 ctggttggagatcttgtacggctgcttcctcgggttcacctcgtcagagcacctctccgg 488
||||||||| |||||||| |||||||||| ||||||||||||||||||||||| ||
Sbjct: 322 ctggttggacatcttgtaaggctgcttccgtgggttcacctcgtcagagcaccttgttgg 381
Query: 489 gacggggtagcccgggaaggacttgcggagggtgcggacgacgtcctcgcggtggagcac 548
||||||||| | |||||| |||| |||| || |||||||||||| |||||| || ||
Sbjct: 382 gacggggtactcggggaagagcttggcgaggatgtggacgacgtcctggcggtgcaggac 441
Query: 549 ggcgtcggcgcagatgtagcggccgg 574
| |||||||| | |||||||||||
Sbjct: 442 gcgctcggcgcaaaggtagcggccgg 467
>gb|BF254781.2|BF254781 HVSMEf0005A11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0005A11f, mRNA sequence
Length = 586
Score = 83.8 bits (42), Expect = 6e-015
Identities = 120/146 (82%)
Strand = Plus / Plus
Query: 429 ctggttggagatcttgtacggctgcttcctcgggttcacctcgtcagagcacctctccgg 488
||||||||| |||||||| |||||||| | ||||||||||||||||||||||| ||
Sbjct: 311 ctggttggacatcttgtaaggctgcttacgagggttcacctcgtcagagcaccttgttgg 370
Query: 489 gacggggtagcccgggaaggacttgcggagggtgcggacgacgtcctcgcggtggagcac 548
||||||||| | |||||| |||| |||| || |||||||||||| |||||| || ||
Sbjct: 371 gacggggtactcggggaagagcttggcgaggatgtggacgacgtcctggcggtgcaggac 430
Query: 549 ggcgtcggcgcagatgtagcggccgg 574
| |||||||| | |||||||||||
Sbjct: 431 gcgctcggcgcataggtagcggccgg 456
>gb|BJ450530.1|BJ450530 BJ450530 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30a24 5', mRNA sequence
Length = 439
Score = 79.8 bits (40), Expect = 9e-014
Identities = 119/146 (81%)
Strand = Plus / Minus
Query: 429 ctggttggagatcttgtacggctgcttcctcgggttcacctcgtcagagcacctctccgg 488
||||||||| |||||||| |||||||||| ||||||||||||||| ||||||| ||
Sbjct: 287 ctggttggacatcttgtaaggctgcttccgtgggttcacctcgtcanagcaccttgttgg 228
Query: 489 gacggggtagcccgggaaggacttgcggagggtgcggacgacgtcctcgcggtggagcac 548
|||| |||| | |||||| |||| ||| || |||||||||||| |||||| || ||
Sbjct: 227 gacgnggtactcggggaagagcttggcgagaatgtggacgacgtcctggcggtgcaggac 168
Query: 549 ggcgtcggcgcagatgtagcggccgg 574
| |||||||||| |||||||||||
Sbjct: 167 gcgctcggcgcagaggtagcggccgg 142
>gb|BJ457952.1|BJ457952 BJ457952 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30a24 3', mRNA sequence
Length = 362
Score = 77.8 bits (39), Expect = 4e-013
Identities = 95/114 (83%)
Strand = Plus / Plus
Query: 429 ctggttggagatcttgtacggctgcttcctcgggttcacctcgtcagagcacctctccgg 488
||||||||| |||||||| |||||||||| ||||||||||||||||||||||| ||
Sbjct: 240 ctggttggacatcttgtaaggctgcttccgtgggttcacctcgtcagagcaccttgttgg 299
Query: 489 gacggggtagcccgggaaggacttgcggagggtgcggacgacgtcctcgcggtg 542
||||||||| | |||||| |||| |||| || ||||||||| || ||||||
Sbjct: 300 gacggggtactcggggaagagcttggcgaggatgtggacgacgtnctggcggtg 353
>gb|CA000262.1|CA000262 HS09C23u HS Hordeum vulgare subsp. vulgare cDNA clone HS09C23
3-PRIME, mRNA sequence
Length = 541
Score = 56.0 bits (28), Expect = 1e-006
Identities = 46/52 (88%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| ||||||||| |||||||||||
Sbjct: 289 gtgaactccaatcccagctccctcagccttcggttggagaacttgtacggct 340
>gb|CA001150.1|CA001150 HS05E05u HS Hordeum vulgare subsp. vulgare cDNA clone HS05E05
3-PRIME, mRNA sequence
Length = 566
Score = 56.0 bits (28), Expect = 1e-006
Identities = 46/52 (88%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| ||||||||| |||||||||||
Sbjct: 291 gtgaactccaatcccagctccctcagccttcggttggagaacttgtacggct 342
>gb|CA010224.1|CA010224 HT11I05u HT Hordeum vulgare subsp. vulgare cDNA clone HT11I05
3-PRIME, mRNA sequence
Length = 447
Score = 56.0 bits (28), Expect = 1e-006
Identities = 46/52 (88%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| ||||||||| |||||||||||
Sbjct: 289 gtgaactccaatcccagctccctcagccttcggttggagaacttgtacggct 340
>gb|BI958488.1|BI958488 HVSMEn0015E11f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0015E11f, mRNA sequence
Length = 620
Score = 54.0 bits (27), Expect = 5e-006
Identities = 114/143 (79%)
Strand = Plus / Minus
Query: 432 gttggagatcttgtacggctgcttcctcgggttcacctcgtcagagcacctctccgggac 491
|||||| |||||||| || |||||| |||||||| |||||||||||||| |||||
Sbjct: 429 gttggacatcttgtaaggatgcttcggtgggttcacatcgtcagagcaccttgttgggac 370
Query: 492 ggggtagcccgggaaggacttgcggagggtgcggacgacgtcctcgcggtggagcacggc 551
|||||| |||||| |||| |||| || ||| |||||||| |||||| || |||
Sbjct: 369 ggggtactaggggaagagcttggagaggatgtggaagacgtcctggcggtgcaggacgcg 310
Query: 552 gtcggcgcagatgtagcggccgg 574
|||||||||| |||||||||||
Sbjct: 309 ctcggcgcagaggtagcggccgg 287
>gb|BQ467661.1|BQ467661 HS04H17r HS Hordeum vulgare subsp. vulgare cDNA clone HS04H17
5-PRIME, mRNA sequence
Length = 486
Score = 48.1 bits (24), Expect = 3e-004
Identities = 36/40 (90%)
Strand = Plus / Minus
Query: 412 cccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||||||||| ||||||||| |||||||||||
Sbjct: 481 cccagctccctcagccttcggttggagaacttgtacggct 442
>gb|BQ663162.1|BQ663162 HU01A05w HU Hordeum vulgare subsp. vulgare cDNA clone HU01A05
3-PRIME, mRNA sequence
Length = 599
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|BQ663264.1|BQ663264 HU01M14w HU Hordeum vulgare subsp. vulgare cDNA clone HU01M14
3-PRIME, mRNA sequence
Length = 585
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 278 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 329
>gb|CA000392.1|CA000392 HS08M20u HS Hordeum vulgare subsp. vulgare cDNA clone HS08M20
3-PRIME, mRNA sequence
Length = 545
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|CA001406.1|CA001406 HS18B01u HS Hordeum vulgare subsp. vulgare cDNA clone HS18B01
3-PRIME, mRNA sequence
Length = 481
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|CA005674.1|CA005674 HU09D12u HU Hordeum vulgare subsp. vulgare cDNA clone HU09D12
3-PRIME, mRNA sequence
Length = 430
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|CA006217.1|CA006217 HU05D06u HU Hordeum vulgare subsp. vulgare cDNA clone HU05D06
3-PRIME, mRNA sequence
Length = 548
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|CA010747.1|CA010747 HT08M24u HT Hordeum vulgare subsp. vulgare cDNA clone HT08M24
3-PRIME, mRNA sequence
Length = 554
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Plus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 272 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 323
>gb|CA013550.1|CA013550 HT08M24r HT Hordeum vulgare subsp. vulgare cDNA clone HT08M24
5-PRIME, mRNA sequence
Length = 544
Score = 48.1 bits (24), Expect = 3e-004
Identities = 45/52 (86%)
Strand = Plus / Minus
Query: 400 gtgaactccaggcccagctccctcagccgctggttggagatcttgtacggct 451
|||||||||| |||||||||||||||| |||| |||| |||||||||||
Sbjct: 273 gtgaactccaatcccagctccctcagccttcggttcgagaacttgtacggct 222
>gb|BM444997.2|BM444997 EBem09_SQ007_C03_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ007_C03 5', mRNA sequence
Length = 322
Score = 40.1 bits (20), Expect = 0.078
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 553 tcggcgcagatgtagcggcc 572
||||||||||||||||||||
Sbjct: 20 tcggcgcagatgtagcggcc 1
>gb|AL504555.1|AL504555 AL504555 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05K08V 5', mRNA sequence
Length = 663
Score = 38.2 bits (19), Expect = 0.31
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 306 cggtgacgccggcgttggg 324
|||||||||||||||||||
Sbjct: 141 cggtgacgccggcgttggg 123
>gb|BF616863.3|BF616863 HVSMEc0013C20f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0013C20f, mRNA sequence
Length = 568
Score = 38.2 bits (19), Expect = 0.31
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 540 gtggagcacggcgtcggcg 558
|||||||||||||||||||
Sbjct: 43 gtggagcacggcgtcggcg 25
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 79,610
Number of Sequences: 312970
Number of extensions: 79610
Number of successful extensions: 24797
Number of sequences better than 0.5: 23
Number of HSP's better than 0.5 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 24761
Number of HSP's gapped (non-prelim): 30
length of query: 581
length of database: 175,134,539
effective HSP length: 19
effective length of query: 562
effective length of database: 169,188,109
effective search space: 95083717258
effective search space used: 95083717258
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)