BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2623104.2.4
(1628 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 167 1e-039
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 167 1e-039
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 167 1e-039
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 167 1e-039
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 139 3e-031
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 137 1e-030
gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow st... 100 3e-019
gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow st... 84 2e-014
gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. v... 56 4e-006
gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke devel... 54 1e-005
gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedl... 44 0.014
gb|AL506416.1|AL506416 AL506416 Hordeum vulgare Barke devel... 40 0.22
gb|BI954356.1|BI954356 HVSMEm0017G14f Hordeum vulgare green... 40 0.22
gb|BI954543.1|BI954543 HVSMEm0018I12f Hordeum vulgare green... 40 0.22
gb|BE601939.3|BE601939 HVSMEh0100F08f Hordeum vulgare 5-45 ... 40 0.22
gb|AV910176.1|AV910176 AV910176 K. Sato unpublished cDNA li... 40 0.22
gb|BJ448141.1|BJ448141 BJ448141 K. Sato unpublished cDNA li... 40 0.22
gb|BJ452289.1|BJ452289 BJ452289 K. Sato unpublished cDNA li... 40 0.22
gb|BJ459840.1|BJ459840 BJ459840 K. Sato unpublished cDNA li... 40 0.22
emb|AJ001268.1|HVPATHPR5 Hordeum vulgare mRNA for basic pat... 40 0.22
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 167 bits (84), Expect = 1e-039
Identities = 161/186 (86%), Gaps = 3/186 (1%)
Strand = Plus / Plus
Query: 944 tccaagatccactacgagaatatcaagatggaggactcagccaaccccatcttcatcgac 1003
||||||||| ||||||||| ||||||||||| ||||| | | |||||||| |||||||
Sbjct: 75 tccaagatcagctacgagaacatcaagatggacgactcgggctaccccatcatcatcgat 134
Query: 1004 atgaagtactgccccaacaagttgtgtactgccaacggcg---cctccaaggtcaccgtc 1060
|||||||||||||||||| | || || ||||| |||| |||||| ||||||||||
Sbjct: 135 cagaagtactgccccaacaaactctgcaccgccaagggcgactcctccagggtcaccgtc 194
Query: 1061 aaggacgtcaccttcaagaacatcaccggcacctcctccaccccggaggccgttagcctg 1120
|||||||| |||||||| |||||||||||||||||||||||||| |||||||| ||||||
Sbjct: 195 aaggacgttaccttcaaaaacatcaccggcacctcctccacccctgaggccgtcagcctg 254
Query: 1121 ctctgc 1126
||||||
Sbjct: 255 ctctgc 260
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 167 bits (84), Expect = 1e-039
Identities = 161/186 (86%), Gaps = 3/186 (1%)
Strand = Plus / Plus
Query: 944 tccaagatccactacgagaatatcaagatggaggactcagccaaccccatcttcatcgac 1003
||||||||| ||||||||| ||||||||||| ||||| | | |||||||| |||||||
Sbjct: 168 tccaagatcagctacgagaacatcaagatggacgactcgggctaccccatcatcatcgat 227
Query: 1004 atgaagtactgccccaacaagttgtgtactgccaacggcg---cctccaaggtcaccgtc 1060
|||||||||||||||||| | || || ||||| |||| |||||| ||||||||||
Sbjct: 228 cagaagtactgccccaacaaactctgcaccgccaagggcgactcctccagggtcaccgtc 287
Query: 1061 aaggacgtcaccttcaagaacatcaccggcacctcctccaccccggaggccgttagcctg 1120
|||||||| |||||||| |||||||||||||||||||||||||| |||||||| ||||||
Sbjct: 288 aaggacgttaccttcaaaaacatcaccggcacctcctccacccctgaggccgtcagcctg 347
Query: 1121 ctctgc 1126
||||||
Sbjct: 348 ctctgc 353
Score = 117 bits (59), Expect = 1e-024
Identities = 83/91 (91%)
Strand = Plus / Plus
Query: 778 cggcgtgacctgcggccctggccacggcatcagcatcggcagcctagggcggtacaagga 837
|||||||||||||||||| |||||||||||||| || |||||||| ||| ||||||||||
Sbjct: 2 cggcgtgacctgcggcccaggccacggcatcagtattggcagcctcgggaggtacaagga 61
Query: 838 cgagaaggacgtcacggacatcaacgtcaag 868
|||||||||||| || |||||| ||||||||
Sbjct: 62 cgagaaggacgtgaccgacatccacgtcaag 92
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 167 bits (84), Expect = 1e-039
Identities = 161/186 (86%), Gaps = 3/186 (1%)
Strand = Plus / Plus
Query: 944 tccaagatccactacgagaatatcaagatggaggactcagccaaccccatcttcatcgac 1003
||||||||| ||||||||| ||||||||||| ||||| | | |||||||| |||||||
Sbjct: 50 tccaagatcagctacgagaacatcaagatggacgactcgggctaccccatcatcatcgat 109
Query: 1004 atgaagtactgccccaacaagttgtgtactgccaacggcg---cctccaaggtcaccgtc 1060
|||||||||||||||||| | || || ||||| |||| |||||| ||||||||||
Sbjct: 110 cagaagtactgccccaacaaactctgcaccgccaagggcgactcctccagggtcaccgtc 169
Query: 1061 aaggacgtcaccttcaagaacatcaccggcacctcctccaccccggaggccgttagcctg 1120
|||||||| |||||||| |||||||||||||||||||||||||| |||||||| ||||||
Sbjct: 170 aaggacgttaccttcaaaaacatcaccggcacctcctccacccctgaggccgtcagcctg 229
Query: 1121 ctctgc 1126
||||||
Sbjct: 230 ctctgc 235
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 167 bits (84), Expect = 1e-039
Identities = 161/186 (86%), Gaps = 3/186 (1%)
Strand = Plus / Plus
Query: 944 tccaagatccactacgagaatatcaagatggaggactcagccaaccccatcttcatcgac 1003
||||||||| ||||||||| ||||||||||| ||||| | | |||||||| |||||||
Sbjct: 110 tccaagatcagctacgagaacatcaagatggacgactcgggctaccccatcatcatcgat 169
Query: 1004 atgaagtactgccccaacaagttgtgtactgccaacggcg---cctccaaggtcaccgtc 1060
|||||||||||||||||| | || || ||||| |||| |||||| ||||||||||
Sbjct: 170 cagaagtactgccccaacaaactctgcaccgccaagggcgactcctccagggtcaccgtc 229
Query: 1061 aaggacgtcaccttcaagaacatcaccggcacctcctccaccccggaggccgttagcctg 1120
|||||||| |||||||| |||||||||||||||||||||||||| |||||||| ||||||
Sbjct: 230 aaggacgttaccttcaaaaacatcaccggcacctcctccacccctgaggccgtcagcctg 289
Query: 1121 ctctgc 1126
||||||
Sbjct: 290 ctctgc 295
Score = 44.1 bits (22), Expect = 0.014
Identities = 31/34 (91%)
Strand = Plus / Plus
Query: 835 ggacgagaaggacgtcacggacatcaacgtcaag 868
||||||||||||||| || |||||| ||||||||
Sbjct: 1 ggacgagaaggacgtgaccgacatccacgtcaag 34
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 139 bits (70), Expect = 3e-031
Identities = 166/198 (83%)
Strand = Plus / Plus
Query: 671 ggcatccacatgggcgactcatccgggatcaccatcaccaacaccgtcattggcgtcggc 730
|||||||||||||||||||| ||| ||| ||||| | ||||| ||| | ||| |||
Sbjct: 2 ggcatccacatgggcgactcgtccaatgtcaacatcatcgacaccaccatcgccgttggc 61
Query: 731 gacgactgcatctccatcggccccgggacctccaaggtgaacatcaccggcgtgacctgc 790
||||| |||||||||||||||||||| || | || ||||||| |||||||||||||
Sbjct: 62 gacgattgcatctccatcggccccggctccaaacaagtcaacatcagcggcgtgacctgc 121
Query: 791 ggccctggccacggcatcagcatcggcagcctagggcggtacaaggacgagaaggacgtc 850
||||| ||||||||||||| || |||||||| ||| ||||||||||||||||||||||
Sbjct: 122 ggcccaagccacggcatcagtattggcagcctcgggaggtacaaggacgagaaggacgtg 181
Query: 851 acggacatcaacgtcaag 868
|| |||||| ||||||||
Sbjct: 182 accgacatccacgtcaag 199
Score = 63.9 bits (32), Expect = 2e-008
Identities = 68/80 (85%)
Strand = Plus / Plus
Query: 944 tccaagatccactacgagaatatcaagatggaggactcagccaaccccatcttcatcgac 1003
||||||||| ||||||||| ||||||||||| |||||| | |||||||| |||||||
Sbjct: 275 tccaagatcagctacgagaacatcaagatggacgactcaagctaccccatcatcatcgat 334
Query: 1004 atgaagtactgccccaacaa 1023
||||||||||||| ||||
Sbjct: 335 cagaagtactgcccccacaa 354
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 137 bits (69), Expect = 1e-030
Identities = 125/143 (87%), Gaps = 3/143 (2%)
Strand = Plus / Plus
Query: 987 accccatcttcatcgacatgaagtactgccccaacaagttgtgtactgccaacggcg--- 1043
|||||||| ||||||| |||||||||||||||||| | || || ||||| ||||
Sbjct: 14 accccatcatcatcgatcagaagtactgccccaacaaactctgcaccgccaagggcgact 73
Query: 1044 cctccaaggtcaccgtcaaggacgtcaccttcaagaacatcaccggcacctcctccaccc 1103
|||||| |||||||||||||||||| |||||||| |||||||||||||||||||||||||
Sbjct: 74 cctccagggtcaccgtcaaggacgttaccttcaaaaacatcaccggcacctcctccaccc 133
Query: 1104 cggaggccgttagcctgctctgc 1126
| |||||||| ||||||||||||
Sbjct: 134 ctgaggccgtcagcctgctctgc 156
>gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_F12 5', mRNA sequence
Length = 311
Score = 99.6 bits (50), Expect = 3e-019
Identities = 101/118 (85%)
Strand = Plus / Plus
Query: 573 tcaactccaagttcttccacatgaacatgtaccggtgcaaggacatgctgatcaaggacg 632
|||| ||||||||||||||||||||||| ||| |||||| | | || | |||| ||||
Sbjct: 160 tcaattccaagttcttccacatgaacatctacgagtgcaaaggcgtgatcgtcaaagacg 219
Query: 633 tgaccgtgacggcgcccggggacagccccaacacggatggcatccacatgggcgactc 690
||| |||| ||||||||||||||||||||||| || ||||||||||||||||||||
Sbjct: 220 tgaaagtgagcgcgcccggggacagccccaacaccgacggcatccacatgggcgactc 277
>gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_B18 5', mRNA sequence
Length = 431
Score = 83.8 bits (42), Expect = 2e-014
Identities = 75/86 (87%)
Strand = Plus / Plus
Query: 188 ggcaagacagacagcacgaaggctgtgcaggaggcatgggcatcggcgtgcggcggcact 247
|||||||| |||||||| || || ||| ||||||||||||| |||||||||||||||||
Sbjct: 190 ggcaagactgacagcaccaaagcggtggaggaggcatgggcttcggcgtgcggcggcaca 249
Query: 248 gggaagcagacaatcctcatacccaa 273
|| |||||||| ||| |||| |||||
Sbjct: 250 ggcaagcagacgatcgtcatccccaa 275
>gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. vulgare cDNA clone HM10P12
3-PRIME, mRNA sequence
Length = 608
Score = 56.0 bits (28), Expect = 4e-006
Identities = 37/40 (92%)
Strand = Plus / Plus
Query: 788 tgcggccctggccacggcatcagcatcggcagcctagggc 827
|||| ||| |||||||||||||||||||||||||| ||||
Sbjct: 366 tgcgacccgggccacggcatcagcatcggcagcctggggc 405
Score = 50.1 bits (25), Expect = 2e-004
Identities = 34/37 (91%)
Strand = Plus / Plus
Query: 643 ggcgcccggggacagccccaacacggatggcatccac 679
|||||| ||||||||||||||||| || |||||||||
Sbjct: 221 ggcgccgggggacagccccaacaccgacggcatccac 257
>gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C10T 5',
mRNA sequence
Length = 507
Score = 54.0 bits (27), Expect = 1e-005
Identities = 30/31 (96%)
Strand = Plus / Plus
Query: 797 ggccacggcatcagcatcggcagcctagggc 827
|||||||||||||||||||||||||| ||||
Sbjct: 14 ggccacggcatcagcatcggcagcctggggc 44
>gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0019E22f, mRNA sequence
Length = 635
Score = 44.1 bits (22), Expect = 0.014
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 650 ggggacagccccaacacggatggcatccac 679
||||||||||||||||| || |||||||||
Sbjct: 540 ggggacagccccaacaccgacggcatccac 569
>gb|AL506416.1|AL506416 AL506416 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY03A18T 5',
mRNA sequence
Length = 677
Score = 40.1 bits (20), Expect = 0.22
Identities = 30/32 (93%), Gaps = 1/32 (3%)
Strand = Plus / Plus
Query: 797 ggccacggcatcag-catcggcagcctagggc 827
|||||||||||||| |||||||||||| ||||
Sbjct: 14 ggccacggcatcaggcatcggcagcctggggc 45
>gb|BI954356.1|BI954356 HVSMEm0017G14f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0017G14f, mRNA sequence
Length = 600
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 178 caccaaggattgcctcaaggagct 201
>gb|BI954543.1|BI954543 HVSMEm0018I12f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0018I12f, mRNA sequence
Length = 659
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 523 caccaaggattgcctcaaggagct 546
>gb|BE601939.3|BE601939 HVSMEh0100F08f Hordeum vulgare 5-45 DAP spike EST library HVcDNA0009
(5 to 45 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEh0100F08f, mRNA sequence
Length = 712
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 516 caccaaggattgcctcaaggagct 539
>gb|AV910176.1|AV910176 AV910176 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak3h20 5', mRNA sequence
Length = 473
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 76 caccaaggattgcctcaaggagct 99
>gb|BJ448141.1|BJ448141 BJ448141 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak18l13 5', mRNA sequence
Length = 561
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 158 caccaaggattgcctcaaggagct 181
>gb|BJ452289.1|BJ452289 BJ452289 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak38h07 5', mRNA sequence
Length = 540
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 137 caccaaggattgcctcaaggagct 160
>gb|BJ459840.1|BJ459840 BJ459840 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak38h07 3', mRNA sequence
Length = 523
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 386 caccaaggattgcctcaaggagct 363
>emb|AJ001268.1|HVPATHPR5 Hordeum vulgare mRNA for basic pathogenesis-related protein, PR5
Length = 867
Score = 40.1 bits (20), Expect = 0.22
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 1231 caccaagggttgcctcaaggagct 1254
|||||||| |||||||||||||||
Sbjct: 462 caccaaggattgcctcaaggagct 485
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 203,567
Number of Sequences: 312970
Number of extensions: 203567
Number of successful extensions: 58144
Number of sequences better than 0.5: 20
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 58065
Number of HSP's gapped (non-prelim): 72
length of query: 1628
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1609
effective length of database: 169,188,109
effective search space: 272223667381
effective search space used: 272223667381
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)