BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619146.2.1
(636 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subs... 165 2e-039
gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, n... 159 1e-037
gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. v... 151 3e-035
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 137 5e-031
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 111 3e-023
gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachi... 92 3e-017
gb|BQ767072.1|BQ767072 EBro08_SQ007_K24_R root, 3 week, dro... 68 4e-010
gb|BM097866.2|BM097866 EBem04_SQ002_P04_R embryo, 12 DPA, n... 58 4e-007
>gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200065F09F1, mRNA sequence
Length = 360
Score = 165 bits (83), Expect = 2e-039
Identities = 170/199 (85%)
Strand = Plus / Minus
Query: 366 cccaccaccttccacgccactgcctgctcgtacgacgcctgccgccccatgctcgacctg 425
|||||||||||||||||||| |||||||| ||||||||||||||||||||||||||| ||
Sbjct: 199 cccaccaccttccacgccaccgcctgctcatacgacgcctgccgccccatgctcgacttg 140
Query: 426 cacacgccaccggggaggtacgccaggccctgctgcatgatcgcggggagctgcgcggac 485
|||||||| |||||||||||||| | ||||||||||||| | || || || ||| ||
Sbjct: 139 cacacgcccccggggaggtacgcgaaaccctgctgcatgaccttggagaactcggcgcac 80
Query: 486 agggccttctggccctcctcgtcgaggaccgcctccagcggcaggtcctggatgttcacc 545
|| ||||| | ||||| ||||| |||| ||||||||||| | |||||| | |||||||
Sbjct: 79 agcgccttgcgcccctcgtcgtccaggatcgcctccagcgagatgtcctgcaggttcacc 20
Query: 546 atcgtcgtctccaggatgt 564
| |||||||||||| ||||
Sbjct: 19 agcgtcgtctccagcatgt 1
>gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_N18 5', mRNA sequence
Length = 540
Score = 159 bits (80), Expect = 1e-037
Identities = 170/200 (85%)
Strand = Plus / Minus
Query: 366 cccaccaccttccacgccactgcctgctcgtacgacgcctgccgccccatgctcgacctg 425
|||||||| ||||||||||| |||||||| ||||||||||||||||||||||||||| ||
Sbjct: 358 cccaccactttccacgccaccgcctgctcatacgacgcctgccgccccatgctcgacttg 299
Query: 426 cacacgccaccggggaggtacgccaggccctgctgcatgatcgcggggagctgcgcggac 485
|||||||| |||||||||||||| | ||||||||||||| | || || || ||| ||
Sbjct: 298 cacacgcccccggggaggtacgcgaaaccctgctgcatgaccttggagaactcggcgcac 239
Query: 486 agggccttctggccctcctcgtcgaggaccgcctccagcggcaggtcctggatgttcacc 545
|| ||||| | ||||| ||||| |||| ||||||||||| | |||||| | |||||||
Sbjct: 238 agcgccttgcgcccctcgtcgtccaggatcgcctccagcgagatgtcctgcaggttcacc 179
Query: 546 atcgtcgtctccaggatgtc 565
| |||||||||||| |||||
Sbjct: 178 agcgtcgtctccagcatgtc 159
Score = 54.0 bits (27), Expect = 6e-006
Identities = 30/31 (96%)
Strand = Plus / Minus
Query: 606 ggcttcagagagcagcagagtatcgcgtcgc 636
||||||||||||||||||||||| |||||||
Sbjct: 121 ggcttcagagagcagcagagtatggcgtcgc 91
>gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
3-PRIME, mRNA sequence
Length = 489
Score = 151 bits (76), Expect = 3e-035
Identities = 160/188 (85%)
Strand = Plus / Plus
Query: 366 cccaccaccttccacgccactgcctgctcgtacgacgcctgccgccccatgctcgacctg 425
|||||||||||||||||||| |||||||| ||||||||||||||||||||||||||| ||
Sbjct: 302 cccaccaccttccacgccaccgcctgctcatacgacgcctgccgccccatgctcgacttg 361
Query: 426 cacacgccaccggggaggtacgccaggccctgctgcatgatcgcggggagctgcgcggac 485
|||||||| |||||||||||||| | ||||||||||||| | || || || ||| ||
Sbjct: 362 cacacgcccccggggaggtacgcgaaaccctgctgcatgaccttggagaactcggcgcac 421
Query: 486 agggccttctggccctcctcgtcgaggaccgcctccagcggcaggtcctggatgttcacc 545
|| ||||| | ||||| ||||| |||| ||||||||||| | |||||| | |||||||
Sbjct: 422 agcgccttgcgcccctcgtcgtccaggatcgcctccagcgagatgtcctgcaggttcacc 481
Query: 546 atcgtcgt 553
| ||||||
Sbjct: 482 agcgtcgt 489
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 137 bits (69), Expect = 5e-031
Identities = 132/153 (86%)
Strand = Plus / Plus
Query: 314 gaaggtccagttggcgagcatgagcgccaggcactgcggggcgccgtcgtcgcccaccac 373
||||| ||||||| ||| ||||| || ||||| ||||| ||| |||||||||||| |||
Sbjct: 278 gaaggaccagttgacgaacatgaaggcgaggcagtgcggcgcgtcgtcgtcgcccagcac 337
Query: 374 cttccacgccactgcctgctcgtacgacgcctgccgccccatgctcgacctgcacacgcc 433
|||||||||||| ||||||||||||||| || |||||| |||||||||| |||||||||
Sbjct: 338 cttccacgccaccgcctgctcgtacgacaccggccgcctcatgctcgacacgcacacgcc 397
Query: 434 accggggaggtacgccaggccctgctgcatgat 466
|| ||||||||||| | ||||||||||||||
Sbjct: 398 gcctgggaggtacgcgaaaccctgctgcatgat 430
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 111 bits (56), Expect = 3e-023
Identities = 109/126 (86%), Gaps = 1/126 (0%)
Strand = Plus / Minus
Query: 342 aggcactgcggggcgccgtcgtcgcccaccaccttccacgccactgcctgctcgtacgac 401
||||| ||||| ||| ||||||||| || ||||||||||||||| |||||||||||||||
Sbjct: 715 aggcagtgcggcgcgtcgtcgtcgcncagcaccttccacgccaccgcctgctcgtacgac 656
Query: 402 gcctgccgcccc-atgctcgacctgcacacgccaccggggaggtacgccaggccctgctg 460
|| |||||||| ||||||||| ||||||||| | ||||||||||| | ||||||||
Sbjct: 655 accggccgcccctatgctcgacacgcacacgccgcntgggaggtacgcgaaaccctgctg 596
Query: 461 catgat 466
||||||
Sbjct: 595 catgat 590
>gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009M03f, mRNA sequence
Length = 718
Score = 91.7 bits (46), Expect = 3e-017
Identities = 78/89 (87%)
Strand = Plus / Minus
Query: 378 cacgccactgcctgctcgtacgacgcctgccgccccatgctcgacctgcacacgccaccg 437
|||||||| || |||||||||||| || ||||||||||||||||| ||||||||| ||
Sbjct: 212 cacgccaccgcntgctcgtacgacaccggccgccccatgctcgacacgcacacgccgcct 153
Query: 438 gggaggtacgccaggccctgctgcatgat 466
||||||||||| | ||||||||||||||
Sbjct: 152 gggaggtacgcgaaaccctgctgcatgat 124
>gb|BQ767072.1|BQ767072 EBro08_SQ007_K24_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ007_K24 5', mRNA sequence
Length = 397
Score = 67.9 bits (34), Expect = 4e-010
Identities = 61/70 (87%)
Strand = Plus / Minus
Query: 353 ggcgccgtcgtcgcccaccaccttccacgccactgcctgctcgtacgacgcctgccgccc 412
||||||| ||||| | | ||||||||||||||| |||| | ||||||||||| |||| ||
Sbjct: 178 ggcgccgccgtcgtcgagcaccttccacgccaccgccttcccgtacgacgccggccggcc 119
Query: 413 catgctcgac 422
||||||||||
Sbjct: 118 catgctcgac 109
>gb|BM097866.2|BM097866 EBem04_SQ002_P04_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ002_P04 5', mRNA sequence
Length = 349
Score = 58.0 bits (29), Expect = 4e-007
Identities = 41/45 (91%)
Strand = Plus / Minus
Query: 366 cccaccaccttccacgccactgcctgctcgtacgacgcctgccgc 410
|||||||| ||||||||||| || ||||| |||||||||||||||
Sbjct: 45 cccaccactttccacgccaccgcttgctcatacgacgcctgccgc 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 76,573
Number of Sequences: 312970
Number of extensions: 76573
Number of successful extensions: 23282
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23262
Number of HSP's gapped (non-prelim): 19
length of query: 636
length of database: 175,134,539
effective HSP length: 19
effective length of query: 617
effective length of database: 169,188,109
effective search space: 104389063253
effective search space used: 104389063253
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)