BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594433.2.1
(766 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV915784.1|AV915784 AV915784 K. Sato unpublished cDNA li... 121 4e-026
gb|BJ468452.1|BJ468452 BJ468452 K. Sato unpublished cDNA li... 121 4e-026
gb|AV916763.1|AV916763 AV916763 K. Sato unpublished cDNA li... 94 8e-018
gb|CK125269.1|CK125269 BES1824107i19 BES1824 Hordeum vulgar... 92 3e-017
gb|AV920899.1|AV920899 AV920899 K. Sato unpublished cDNA li... 80 1e-013
gb|AV922058.1|AV922058 AV922058 K. Sato unpublished cDNA li... 80 1e-013
gb|AV921048.1|AV921048 AV921048 K. Sato unpublished cDNA li... 72 3e-011
gb|AV922079.1|AV922079 AV922079 K. Sato unpublished cDNA li... 72 3e-011
gb|AJ462078.1|AJ462078 AJ462078 S00002 Hordeum vulgare subs... 70 1e-010
gb|AV915931.1|AV915931 AV915931 K. Sato unpublished cDNA li... 66 2e-009
>gb|AV915784.1|AV915784 AV915784 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15p23 5', mRNA sequence
Length = 613
Score = 121 bits (61), Expect = 4e-026
Identities = 254/317 (80%), Gaps = 1/317 (0%)
Strand = Plus / Minus
Query: 420 acgctgacgcggaacaggtgaggaacccagg-tggcttccttgagcttcagagcataact 478
|||||||| ||||||||||| |||||||||| |||||||||| ||||| || | |
Sbjct: 533 acgctgacacggaacaggtggggaacccagggtggcttccttcagcttgagctggaacat 474
Query: 479 gtcgtccccctcctctttcctgatccgatcaagctcgtcggcgctgcagccaatgatctt 538
||| || ||||||||||| |||||||||||||| | || || |||||||| | ||| |
Sbjct: 473 gtcatcgccctcctcttttctgatccgatcaagattatctgcactgcagccgacaatcct 414
Query: 539 ctctgcatgctcgttgaacacagagacccatgcctcgccagtaggatcggagaccttgac 598
|||||| || || ||||| | || | ||| ||||| || || ||||| ||||||||||
Sbjct: 413 ctctgcttggtcattgaaaagggacagccaggcctcacctgttggatcagagaccttgat 354
Query: 599 gaccatgatgtatctcagtgagcattccgagtcattcttttggcatccctcgcaccagta 658
||||||||||| |||| ||||| ||| | ||||| ||||| || || ||||||||
Sbjct: 353 caccatgatgtacctcaacgagcactcctcatagttcttctggcacccttcacaccagta 294
Query: 659 tccagatccaaaagtttcagtcaccttcttgttgcaggtcttgcaagcacggtaccacat 718
||| ||||| | | || |||||||||||||||||||| ||||||| |||||||||||
Sbjct: 293 tcctgatccgagtgcctcggtcaccttcttgttgcaggtggtgcaagcgcggtaccacat 234
Query: 719 gttctggtcaggcttga 735
| |||||||||||||||
Sbjct: 233 ggtctggtcaggcttga 217
>gb|BJ468452.1|BJ468452 BJ468452 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38n24 3', mRNA sequence
Length = 739
Score = 121 bits (61), Expect = 4e-026
Identities = 254/317 (80%), Gaps = 1/317 (0%)
Strand = Plus / Plus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagcttcagagcataactg 479
|||||||| ||||||||||| |||||||||||||||||||| ||||| || | ||
Sbjct: 377 acgctgacacggaacaggtggggaacccaggtggcttccttcagcttgagctggaacatg 436
Query: 480 tcgtccccctcctctttcctgatccgatcaagctcgtcggcgctgcagccaatgatcttc 539
|| || ||||||||||| |||||||||||||| | || || |||||||| | ||| ||
Sbjct: 437 tcatcgccctcctcttttctgatccgatcaagattatctgcactgcagccgacaatcctc 496
Query: 540 tctgcatgctcgttgaacacagagacccatgcctcgccagtaggatcggagaccttgacg 599
||||| || || ||||| | || | ||| ||||| || || ||||| ||||||||||
Sbjct: 497 tctgcttggtcattgaaaagggacagccaggcctcacctgttggatcagagaccttgatc 556
Query: 600 accatgatgtatctcagtgagcattccgagtcattcttttggcatccctcgcaccagtat 659
||||||||||| |||| ||||| ||| | ||||| ||||| || || |||||||||
Sbjct: 557 accatgatgtacctcaacgagcactcctcatagttcttctggcacccttcacaccagtat 616
Query: 660 ccagatccaaaagtttcagtcacctt-cttgttgcaggtcttgcaagcacggtaccacat 718
|| ||||| | | || |||||||| |||||||||||| ||||||| |||||||||||
Sbjct: 617 cctgatccgagtgcctcggtcacctttcttgttgcaggtggtgcaagcgcggtaccacat 676
Query: 719 gttctggtcaggcttga 735
| |||||||||||||||
Sbjct: 677 ggtctggtcaggcttga 693
>gb|AV916763.1|AV916763 AV916763 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j02 5', mRNA sequence
Length = 573
Score = 93.7 bits (47), Expect = 8e-018
Identities = 201/251 (80%), Gaps = 1/251 (0%)
Strand = Plus / Minus
Query: 486 ccctcctctttcctgatccgatcaagctcgtcggcgctgca-gccaatgatcttctctgc 544
||||||||||| |||||||||||||| | || || ||||| ||| | ||| |||||||
Sbjct: 545 ccctcctcttttctgatccgatcaagattatctgcactgcaagccgacaatcctctctgc 486
Query: 545 atgctcgttgaacacagagacccatgcctcgccagtaggatcggagaccttgacgaccat 604
|| || ||||| | || | ||| ||||| || || ||||| |||||||||| |||||
Sbjct: 485 ttggtcattgaaaagggacagccaggcctcacctgttggatcagagaccttgatcaccat 426
Query: 605 gatgtatctcagtgagcattccgagtcattcttttggcatccctcgcaccagtatccaga 664
|||||| |||| ||||| ||| | ||||| ||||| || || ||||||||||| ||
Sbjct: 425 gatgtacctcaacgagcactcctcatagttcttctggcacccttcacaccagtatcctga 366
Query: 665 tccaaaagtttcagtcaccttcttgttgcaggtcttgcaagcacggtaccacatgttctg 724
||| | | || |||||||||||||||||||| ||||||| |||||||||||| ||||
Sbjct: 365 tccgagtgcctcggtcaccttcttgttgcaggtggtgcaagcgcggtaccacatggtctg 306
Query: 725 gtcaggcttga 735
|||||||||||
Sbjct: 305 gtcaggcttga 295
>gb|CK125269.1|CK125269 BES1824107i19 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010I197 5-PRIME, mRNA sequence
Length = 917
Score = 91.7 bits (46), Expect = 3e-017
Identities = 127/154 (82%)
Strand = Plus / Minus
Query: 582 ggatcggagaccttgacgaccatgatgtatctcagtgagcattccgagtcattcttttgg 641
||||| |||||||||| ||||||||||| |||| ||||| ||| | ||||| |||
Sbjct: 785 ggatcagagaccttgatcaccatgatgtacctcaacgagcactcctcatagttcttctgg 726
Query: 642 catccctcgcaccagtatccagatccaaaagtttcagtcaccttcttgttgcaggtcttg 701
|| || || ||||||||||| ||||| | | || |||||||||||||||||||| ||
Sbjct: 725 cacccttcacaccagtatcctgatccgagtgcctcggtcaccttcttgttgcaggtggtg 666
Query: 702 caagcacggtaccacatgttctggtcaggcttga 735
||||| |||||||||||| |||||||||||||||
Sbjct: 665 caagcgcggtaccacatggtctggtcaggcttga 632
>gb|AV920899.1|AV920899 AV920899 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15p23 3', mRNA sequence
Length = 648
Score = 79.8 bits (40), Expect = 1e-013
Identities = 157/196 (80%)
Strand = Plus / Plus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagcttcagagcataactg 479
|||||||| ||||||||||| |||||||||||||||||||| ||||| || | ||
Sbjct: 376 acgctgacacggaacaggtggggaacccaggtggcttccttcagcttgagctggaacatg 435
Query: 480 tcgtccccctcctctttcctgatccgatcaagctcgtcggcgctgcagccaatgatcttc 539
|| || ||||||||||| |||||||||||||| | || || |||||||| | ||| ||
Sbjct: 436 tcatcgccctcctcttttctgatccgatcaagattatctgcactgcagccgacaatcctc 495
Query: 540 tctgcatgctcgttgaacacagagacccatgcctcgccagtaggatcggagaccttgacg 599
||||| || || ||||| | || | ||| ||||| || || ||||| ||||||||||
Sbjct: 496 tctgcttggtcattgaaaagggacagccaggcctcacctgttggatcagagaccttgatc 555
Query: 600 accatgatgtatctca 615
||||||||||| ||||
Sbjct: 556 accatgatgtacctca 571
>gb|AV922058.1|AV922058 AV922058 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j02 3', mRNA sequence
Length = 664
Score = 79.8 bits (40), Expect = 1e-013
Identities = 157/196 (80%)
Strand = Plus / Plus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagcttcagagcataactg 479
|||||||| ||||||||||| |||||||||||||||||||| ||||| || | ||
Sbjct: 402 acgctgacacggaacaggtggggaacccaggtggcttccttcagcttgagctggaacatg 461
Query: 480 tcgtccccctcctctttcctgatccgatcaagctcgtcggcgctgcagccaatgatcttc 539
|| || ||||||||||| |||||||||||||| | || || |||||||| | ||| ||
Sbjct: 462 tcatcgccctcctcttttctgatccgatcaagattatctgcactgcagccgacaatcctc 521
Query: 540 tctgcatgctcgttgaacacagagacccatgcctcgccagtaggatcggagaccttgacg 599
||||| || || ||||| | || | ||| ||||| || || ||||| ||||||||||
Sbjct: 522 tctgcttggtcattgaaaagggacagccaggcctcacctgttggatcagagaccttgatc 581
Query: 600 accatgatgtatctca 615
||||||||||| ||||
Sbjct: 582 accatgatgtacctca 597
>gb|AV921048.1|AV921048 AV921048 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16l01 3', mRNA sequence
Length = 651
Score = 71.9 bits (36), Expect = 3e-011
Identities = 78/92 (84%)
Strand = Plus / Plus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagcttcagagcataactg 479
|||||||| ||||||||||| |||||||||||||||||||| ||||| || | ||
Sbjct: 376 acgctgacacggaacaggtggggaacccaggtggcttccttcagcttgagctggaacatg 435
Query: 480 tcgtccccctcctctttcctgatccgatcaag 511
|| || ||||||||||| ||||||||||||||
Sbjct: 436 tcatcgccctcctcttttctgatccgatcaag 467
>gb|AV922079.1|AV922079 AV922079 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j24 3', mRNA sequence
Length = 534
Score = 71.9 bits (36), Expect = 3e-011
Identities = 78/92 (84%)
Strand = Plus / Plus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagcttcagagcataactg 479
|||||||| ||||||||||| |||||||||||||||||||| ||||| || | ||
Sbjct: 388 acgctgacacggaacaggtggggaacccaggtggcttccttcagcttgagctggaacatg 447
Query: 480 tcgtccccctcctctttcctgatccgatcaag 511
|| || ||||||||||| ||||||||||||||
Sbjct: 448 tcatcgccctcctcttttctgatccgatcaag 479
>gb|AJ462078.1|AJ462078 AJ462078 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200004D02F1, mRNA sequence
Length = 300
Score = 69.9 bits (35), Expect = 1e-010
Identities = 44/47 (93%)
Strand = Plus / Minus
Query: 420 acgctgacgcggaacaggtgaggaacccaggtggcttccttgagctt 466
|||||||| ||||||||||| |||||||||||||||||||| |||||
Sbjct: 96 acgctgacacggaacaggtggggaacccaggtggcttccttcagctt 50
>gb|AV915931.1|AV915931 AV915931 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16l01 5', mRNA sequence
Length = 605
Score = 65.9 bits (33), Expect = 2e-009
Identities = 45/49 (91%)
Strand = Plus / Minus
Query: 687 ttgttgcaggtcttgcaagcacggtaccacatgttctggtcaggcttga 735
||||||||||| ||||||| |||||||||||| |||||||||||||||
Sbjct: 605 ttgttgcaggtggtgcaagcgcggtaccacatggtctggtcaggcttga 557
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 72,022
Number of Sequences: 312970
Number of extensions: 72022
Number of successful extensions: 21742
Number of sequences better than 0.5: 10
Number of HSP's better than 0.5 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 21701
Number of HSP's gapped (non-prelim): 28
length of query: 766
length of database: 175,134,539
effective HSP length: 19
effective length of query: 747
effective length of database: 169,188,109
effective search space: 126383517423
effective search space used: 126383517423
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)