BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594405.2.1
(637 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF259812.2|BF259812 HVSMEf0020F08f Hordeum vulgare seedl... 113 7e-024
gb|BF254118.2|BF254118 HVSMEf0003B05f Hordeum vulgare seedl... 38 0.34
gb|BF258244.3|BF258244 HVSMEf0015D10f Hordeum vulgare seedl... 38 0.34
>gb|BF259812.2|BF259812 HVSMEf0020F08f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020F08f, mRNA sequence
Length = 802
Score = 113 bits (57), Expect = 7e-024
Identities = 156/185 (84%), Gaps = 3/185 (1%)
Strand = Plus / Minus
Query: 451 ttcccggaggaacacctctagatcatcagctc-gtatgtgctcgttctct-gctgtgaca 508
|||||||||| ||||||| |||||||| || | | ||||| |||||||| || | ||||
Sbjct: 459 ttcccggaggcacacctcgagatcatccgccccggatgtgttcgttctcccgccgagaca 400
Query: 509 tccaactcagtaatccagatcgggaga-cccagtatggcgagcttgtccagggaatcaca 567
|||| |||||| ||||| || ||||| |||||||| || |||||||| |||||||| ||
Sbjct: 399 tccagctcagtgatccaaatagggagggcccagtatcgccagcttgtctagggaatcgca 340
Query: 568 tatgatttctcctaccggatggctgatatgaccttgcacaccgatcccaccaactggagc 627
|||||| || || || ||||||||||||||||||||||| || ||||||||||| || ||
Sbjct: 339 tatgatatcccccactggatggctgatatgaccttgcacgccaatcccaccaaccggggc 280
Query: 628 gcccc 632
|||||
Sbjct: 279 gcccc 275
Score = 50.1 bits (25), Expect = 9e-005
Identities = 31/33 (93%)
Strand = Plus / Minus
Query: 386 cgaaacataaacatctcccagaatccccagagg 418
|||||||| | ||||||||||||||||||||||
Sbjct: 524 cgaaacatgaccatctcccagaatccccagagg 492
Score = 42.1 bits (21), Expect = 0.022
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 301 ggtcagccactcttgtttgagagcaaggtacct 333
|||||||||||| ||||| ||||||||| ||||
Sbjct: 610 ggtcagccactcctgtttcagagcaaggaacct 578
>gb|BF254118.2|BF254118 HVSMEf0003B05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003B05f, mRNA sequence
Length = 842
Score = 38.2 bits (19), Expect = 0.34
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 355 cccatcagcatcgaccaga 373
|||||||||||||||||||
Sbjct: 260 cccatcagcatcgaccaga 278
>gb|BF258244.3|BF258244 HVSMEf0015D10f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015D10f, mRNA sequence
Length = 752
Score = 38.2 bits (19), Expect = 0.34
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 355 cccatcagcatcgaccaga 373
|||||||||||||||||||
Sbjct: 390 cccatcagcatcgaccaga 408
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 49,875
Number of Sequences: 312970
Number of extensions: 49875
Number of successful extensions: 11061
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11055
Number of HSP's gapped (non-prelim): 6
length of query: 637
length of database: 175,134,539
effective HSP length: 19
effective length of query: 618
effective length of database: 169,188,109
effective search space: 104558251362
effective search space used: 104558251362
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)