BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2568371.2.1
(782 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL503953.1|AL503953 AL503953 Hordeum vulgare Barke roots... 321 2e-086
gb|BG417206.1|BG417206 HVSMEk0016L20f Hordeum vulgare testa... 321 2e-086
gb|AV911878.1|AV911878 AV911878 K. Sato unpublished cDNA li... 321 2e-086
gb|AV920631.1|AV920631 AV920631 K. Sato unpublished cDNA li... 321 2e-086
gb|BJ468052.1|BJ468052 BJ468052 K. Sato unpublished cDNA li... 321 2e-086
gb|AV915531.1|AV915531 AV915531 K. Sato unpublished cDNA li... 307 3e-082
gb|BQ762609.1|BQ762609 EBro02_SQ004_O10_R root, 3 week, hyd... 252 1e-065
gb|BG414869.2|BG414869 HVSMEk0004A12f Hordeum vulgare testa... 210 5e-053
gb|BM442768.2|BM442768 EBro02_SQ001_C12_R root, 3 week, hyd... 121 4e-026
gb|BQ754945.1|BQ754945 EBed02_SQ003_E09_R endosperm, 8 DPA,... 82 3e-014
gb|AL500941.1|AL500941 AL500941 Hordeum vulgare Barke roots... 62 3e-008
gb|BQ661800.1|BQ661800 HP01F17w HP Hordeum vulgare subsp. v... 52 3e-005
gb|BQ659473.1|BQ659473 HE01I09u HE Hordeum vulgare subsp. v... 48 4e-004
gb|BF623736.2|BF623736 HVSMEa0005E15f Hordeum vulgare seedl... 42 0.027
gb|BG344434.1|BG344434 HVSMEg0009E05f Hordeum vulgare pre-a... 42 0.027
gb|BE216883.1|BE216883 HV_CEb0011P02f Hordeum vulgare seedl... 42 0.027
gb|BQ765084.1|BQ765084 EBca01_SQ005_B22_R carpel, pre-anthe... 42 0.027
gb|CB883585.1|CB883585 HQ02L05w HQ Hordeum vulgare subsp. v... 42 0.027
gb|CA029566.1|CA029566 HZ65J09r HZ Hordeum vulgare subsp. v... 38 0.42
>gb|AL503953.1|AL503953 AL503953 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW03P07T 5', mRNA sequence
Length = 638
Score = 321 bits (162), Expect = 2e-086
Identities = 198/210 (94%)
Strand = Plus / Plus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 239 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 298
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 299 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 358
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcac 180
|| |||||| ||||| || || |||||||||||||||||||| |||||||||||||||||
Sbjct: 359 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 418
Query: 181 cgccgtgctgtacatcaacataccccacat 210
||||||||| ||||||||||| ||||||||
Sbjct: 419 cgccgtgctctacatcaacatcccccacat 448
>gb|BG417206.1|BG417206 HVSMEk0016L20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016L20f, mRNA sequence
Length = 472
Score = 321 bits (162), Expect = 2e-086
Identities = 198/210 (94%)
Strand = Plus / Plus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 69 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 128
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 129 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 188
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcac 180
|| |||||| ||||| || || |||||||||||||||||||| |||||||||||||||||
Sbjct: 189 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 248
Query: 181 cgccgtgctgtacatcaacataccccacat 210
||||||||| ||||||||||| ||||||||
Sbjct: 249 cgccgtgctctacatcaacatcccccacat 278
>gb|AV911878.1|AV911878 AV911878 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak1o11 3', mRNA sequence
Length = 558
Score = 321 bits (162), Expect = 2e-086
Identities = 198/210 (94%)
Strand = Plus / Minus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 425 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 366
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 365 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 306
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcac 180
|| |||||| ||||| || || |||||||||||||||||||| |||||||||||||||||
Sbjct: 305 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 246
Query: 181 cgccgtgctgtacatcaacataccccacat 210
||||||||| ||||||||||| ||||||||
Sbjct: 245 cgccgtgctctacatcaacatcccccacat 216
>gb|AV920631.1|AV920631 AV920631 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14p10 3', mRNA sequence
Length = 599
Score = 321 bits (162), Expect = 2e-086
Identities = 198/210 (94%)
Strand = Plus / Minus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 464 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 405
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 404 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 345
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcac 180
|| |||||| ||||| || || |||||||||||||||||||| |||||||||||||||||
Sbjct: 344 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 285
Query: 181 cgccgtgctgtacatcaacataccccacat 210
||||||||| ||||||||||| ||||||||
Sbjct: 284 cgccgtgctctacatcaacatcccccacat 255
>gb|BJ468052.1|BJ468052 BJ468052 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n24 3', mRNA sequence
Length = 525
Score = 321 bits (162), Expect = 2e-086
Identities = 198/210 (94%)
Strand = Plus / Minus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 471 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 412
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 411 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 352
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcac 180
|| |||||| ||||| || || |||||||||||||||||||| |||||||||||||||||
Sbjct: 351 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 292
Query: 181 cgccgtgctgtacatcaacataccccacat 210
||||||||| ||||||||||| ||||||||
Sbjct: 291 cgccgtgctctacatcaacatcccccacat 262
>gb|AV915531.1|AV915531 AV915531 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14p10 5', mRNA sequence
Length = 629
Score = 307 bits (155), Expect = 3e-082
Identities = 198/211 (93%), Gaps = 1/211 (0%)
Strand = Plus / Plus
Query: 1 cgtggccttcgccaaggtgctggacggcgagtggacgcactggctcaaggtggccggcgg 60
|||||||||||||||||| || ||||||||||||||||||||||||||||| ||||||||
Sbjct: 323 cgtggccttcgccaaggttctcgacggcgagtggacgcactggctcaaggtcgccggcgg 382
Query: 61 cgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggg 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 383 cgtcttcttcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggg 442
Query: 121 gaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatca- 179
|| |||||| ||||| || || |||||||||||||||||||| ||||||||||||||||
Sbjct: 443 gaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcac 502
Query: 180 ccgccgtgctgtacatcaacataccccacat 210
|||||||||| ||||||||||| ||||||||
Sbjct: 503 ccgccgtgctctacatcaacatcccccacat 533
>gb|BQ762609.1|BQ762609 EBro02_SQ004_O10_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ004_O10 5', mRNA sequence
Length = 649
Score = 252 bits (127), Expect = 1e-065
Identities = 157/167 (94%)
Strand = Plus / Plus
Query: 44 ctcaaggtggccggcggcgtcttcttcaacttctgggtgctgttccacctctaccccttc 103
|||||||| |||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 1 ctcaaggtcgccggcggcgtcttcttcaacttctgggtgctcttccacctctaccccttc 60
Query: 104 gccaagggcatcctggggaggcacggcaagacccccgtggtggtgctcgtctggtgggcc 163
||||||||||||||||||| |||||| ||||| || || ||||||||||||||||||||
Sbjct: 61 gccaagggcatcctggggaagcacggaaagacgccagtcgtggtgctcgtctggtgggca 120
Query: 164 ttcaccttcgtcatcaccgccgtgctgtacatcaacataccccacat 210
|||||||||||||||||||||||||| ||||||||||| ||||||||
Sbjct: 121 ttcaccttcgtcatcaccgccgtgctctacatcaacatcccccacat 167
>gb|BG414869.2|BG414869 HVSMEk0004A12f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0004A12f, mRNA sequence
Length = 665
Score = 210 bits (106), Expect = 5e-053
Identities = 133/142 (93%)
Strand = Plus / Plus
Query: 69 tcaacttctgggtgctgttccacctctaccccttcgccaagggcatcctggggaggcacg 128
|||||||||||||||| ||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 1 tcaacttctgggtgctcttccacctctaccccttcgccaagggcatcctggggaagcacg 60
Query: 129 gcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcatcaccgccgtgc 188
| ||||| || || |||||||||||||||||||| |||||||||||||||||||||||||
Sbjct: 61 gaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcatcaccgccgtgc 120
Query: 189 tgtacatcaacataccccacat 210
| ||||||||||| ||||||||
Sbjct: 121 tctacatcaacatcccccacat 142
>gb|BM442768.2|BM442768 EBro02_SQ001_C12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ001_C12 5', mRNA sequence
Length = 490
Score = 121 bits (61), Expect = 4e-026
Identities = 85/93 (91%)
Strand = Plus / Plus
Query: 118 ggggaggcacggcaagacccccgtggtggtgctcgtctggtgggccttcaccttcgtcat 177
||||| |||||| ||||| || || |||||||||||||||||||| ||||||||||||||
Sbjct: 1 ggggaagcacggaaagacgccagtcgtggtgctcgtctggtgggcattcaccttcgtcat 60
Query: 178 caccgccgtgctgtacatcaacataccccacat 210
|||||||||||| ||||||||||| ||||||||
Sbjct: 61 caccgccgtgctctacatcaacatcccccacat 93
>gb|BQ754945.1|BQ754945 EBed02_SQ003_E09_R endosperm, 8 DPA, no treatment, cv Optic, EBed02
Hordeum vulgare subsp. vulgare cDNA clone
EBed02_SQ003_E09 5', mRNA sequence
Length = 383
Score = 81.8 bits (41), Expect = 3e-014
Identities = 59/65 (90%)
Strand = Plus / Plus
Query: 56 ggcggcgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatc 115
||||||| |||||||| |||||| ||||||| ||||||||||||||||||||| ||| ||
Sbjct: 314 ggcggcgccttcttcagcttctgagtgctgtgccacctctaccccttcgccaaaggcctc 373
Query: 116 ctggg 120
|||||
Sbjct: 374 ctggg 378
>gb|AL500941.1|AL500941 AL500941 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02H11u 3', mRNA sequence
Length = 646
Score = 61.9 bits (31), Expect = 3e-008
Identities = 49/54 (90%), Gaps = 1/54 (1%)
Strand = Plus / Minus
Query: 154 ctggtgggccttcaccttcgtcatca-ccgccgtgctgtacatcaacatacccc 206
||||||||| |||||||||||||| | |||||||||| ||||||||||| ||||
Sbjct: 584 ctggtgggcattcaccttcgtcataaaccgccgtgctntacatcaacatccccc 531
>gb|BQ661800.1|BQ661800 HP01F17w HP Hordeum vulgare subsp. vulgare cDNA clone HP01F17
3-PRIME, mRNA sequence
Length = 622
Score = 52.0 bits (26), Expect = 3e-005
Identities = 56/66 (84%)
Strand = Plus / Minus
Query: 56 ggcggcgtcttcttcaacttctgggtgctgttccacctctaccccttcgccaagggcatc 115
||||| |||||||||| ||||||||| ||| ||||| ||||| |||||||||||| ||
Sbjct: 441 ggcggtgtcttcttcagcttctgggtactggctcacctgtacccgttcgccaagggcctc 382
Query: 116 ctgggg 121
|||||
Sbjct: 381 atgggg 376
>gb|BQ659473.1|BQ659473 HE01I09u HE Hordeum vulgare subsp. vulgare cDNA clone HE01I09
3-PRIME, mRNA sequence
Length = 559
Score = 48.1 bits (24), Expect = 4e-004
Identities = 32/35 (91%)
Strand = Plus / Minus
Query: 176 atcaccgccgtgctgtacatcaacataccccacat 210
|||||||||||||| ||||||||||| | ||||||
Sbjct: 559 atcaccgccgtgctctacatcaacatccnccacat 525
>gb|BF623736.2|BF623736 HVSMEa0005E15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0005E15f, mRNA sequence
Length = 494
Score = 42.1 bits (21), Expect = 0.027
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 212 cacggccccggcggcaagcacggcggcgc 240
||||||| ||||||||||||||||||||
Sbjct: 212 cacggccgaggcggcaagcacggcggcgc 184
>gb|BG344434.1|BG344434 HVSMEg0009E05f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009E05f, mRNA sequence
Length = 797
Score = 42.1 bits (21), Expect = 0.027
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 212 cacggccccggcggcaagcacggcggcgc 240
||||||| ||||||||||||||||||||
Sbjct: 216 cacggccgaggcggcaagcacggcggcgc 188
>gb|BE216883.1|BE216883 HV_CEb0011P02f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0011P02f, mRNA sequence
Length = 969
Score = 42.1 bits (21), Expect = 0.027
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 212 cacggccccggcggcaagcacggcggcgc 240
||||||| ||||||||||||||||||||
Sbjct: 230 cacggccgaggcggcaagcacggcggcgc 202
>gb|BQ765084.1|BQ765084 EBca01_SQ005_B22_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_B22 5', mRNA sequence
Length = 483
Score = 42.1 bits (21), Expect = 0.027
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 212 cacggccccggcggcaagcacggcggcgc 240
||||||| ||||||||||||||||||||
Sbjct: 211 cacggccgaggcggcaagcacggcggcgc 183
>gb|CB883585.1|CB883585 HQ02L05w HQ Hordeum vulgare subsp. vulgare cDNA clone HQ02L05
3-PRIME, mRNA sequence
Length = 576
Score = 42.1 bits (21), Expect = 0.027
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 212 cacggccccggcggcaagcacggcggcgc 240
||||||| ||||||||||||||||||||
Sbjct: 236 cacggccgaggcggcaagcacggcggcgc 208
>gb|CA029566.1|CA029566 HZ65J09r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ65J09
5-PRIME, mRNA sequence
Length = 540
Score = 38.2 bits (19), Expect = 0.42
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 220 cggcggcaagcacggcggcgcga 242
|||||||||||| ||||||||||
Sbjct: 346 cggcggcaagcagggcggcgcga 368
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 106,912
Number of Sequences: 312970
Number of extensions: 106912
Number of successful extensions: 32641
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 32578
Number of HSP's gapped (non-prelim): 49
length of query: 782
length of database: 175,134,539
effective HSP length: 19
effective length of query: 763
effective length of database: 169,188,109
effective search space: 129090527167
effective search space used: 129090527167
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)