BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521459.2.4
(760 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA li... 426 e-118
gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA li... 424 e-117
gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-a... 307 3e-082
gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedl... 293 4e-078
gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA li... 210 5e-053
gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedl... 163 1e-038
gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa... 131 4e-029
gb|BE216096.1|BE216096 HV_CEb0009G14f Hordeum vulgare seedl... 90 1e-016
gb|BI960271.1|BI960271 HVSMEn0024A19f Hordeum vulgare rachi... 78 5e-013
gb|AV921077.1|AV921077 AV921077 K. Sato unpublished cDNA li... 52 3e-005
gb|BU995988.1|BU995988 HM12A03r HM Hordeum vulgare subsp. v... 52 3e-005
gb|BE455736.3|BE455736 HVSMEg0019G20f Hordeum vulgare pre-a... 46 0.002
gb|BI952463.1|BI952463 HVSMEm0006I19f Hordeum vulgare green... 40 0.10
>gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 5', mRNA sequence
Length = 543
Score = 426 bits (215), Expect = e-118
Identities = 459/538 (85%), Gaps = 3/538 (0%)
Strand = Plus / Minus
Query: 208 ttggttagcggcatgattctgtagctgtacagctcggcgaccaagctcggaacgatcttg 267
||||||||||||||||| |||| ||||||||| | ||||||| ||| || |||||||
Sbjct: 540 ttggttagcggcatgatcctgttgctgtacagtccagcgaccaggctgggcctgatcttg 481
Query: 268 tacaccggtgggctgccgttgtcctccacaccgatgttcacctcttgcccgaagatcttg 327
||||| || | |||| ||| | || | || ||||||||||| || || || |||||
Sbjct: 480 tacacagg--gcatgcccttggcttcagctcctatgttcacctcctgtccaaatatctta 423
Query: 328 gctttggcctccaccctgtgttcgatagcacgctcc-accgtttcatatgtgagaaggtt 386
|| |||| ||||||| |||||||| ||||||||| ||||| || || ||||| || |
Sbjct: 422 gccttggtttccacccgatgttcgatggcacgctccgaccgtctcgtaggtgaggagctg 363
Query: 387 catgagttgatcacggtcttgggctattatggctggcgtgtaagcttcaggggactcttg 446
||| |||||||||| ||| || ||| |||| || || |||||||||||||||||||||||
Sbjct: 362 cattagttgatcaccgtcctgagcttttattgcaggtgtgtaagcttcaggggactcttg 303
Query: 447 aaacttggcctctgccacaaacttcccatagtggatccttctggagagtgcctgcaagca 506
|||||||||||||||||||||||||||||||||||| ||| | |||||||||||||||||
Sbjct: 302 aaacttggcctctgccacaaacttcccatagtggattcttttagagagtgcctgcaagca 243
Query: 507 ggttgtgtcacaaagagcactggatccagcattaccatcacttccttctttcaccaatct 566
||| ||||||||||||||||||||||||| ||| ||||||||||||||||||||||| ||
Sbjct: 242 ggtcgtgtcacaaagagcactggatccagaatttccatcacttccttctttcaccaacct 183
Query: 567 tggaagaagttcatcaaaatacattttccaaatctctttgttgatattgatagaatcagc 626
||||| || ||||||||| ||||||||||||||||||||||||||||| || ||||||||
Sbjct: 182 tggaataacttcatcaaagtacattttccaaatctctttgttgatattaatggaatcagc 123
Query: 627 aatgggatgcaaaacccttgggtattgcataggtggcaaccggggctcaggcagatcctt 686
||| ||||||| |||| |||| || || ||||| |||||| | | |||||||||||| |
Sbjct: 122 aataggatgcagaaccgttggatactggataggcggcaacagtgtctcaggcagatcttc 63
Query: 687 ggaaaagaaagggtgctcatctgggcccttgtatctcccaacctgtgcatggagcttt 744
| |||||| |||||||||||||||| ||||||||||||||||| ||| || ||||||
Sbjct: 62 tggaaagaatgggtgctcatctgggctcttgtatctcccaacctttgcgtgtagcttt 5
>gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16n12 5', mRNA sequence
Length = 470
Score = 424 bits (214), Expect = e-117
Identities = 376/430 (87%)
Strand = Plus / Minus
Query: 301 atgttcacctcttgcccgaagatcttggctttggcctccaccctgtgttcgatagcacgc 360
||||||||||| || || || ||||| || |||| ||||||| |||||||| ||||||
Sbjct: 430 atgttcacctcctgtccaaatatcttagccttggtttccacccgatgttcgatggcacgc 371
Query: 361 tccaccgtttcatatgtgagaaggttcatgagttgatcacggtcttgggctattatggct 420
|| ||||| || || ||||| || | ||| |||||||||| ||| || ||| |||| ||
Sbjct: 370 tcgaccgtctcgtaggtgaggagctgcattagttgatcaccgtcctgagcttttattgca 311
Query: 421 ggcgtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtgg 480
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 310 ggtgtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtgg 251
Query: 481 atccttctggagagtgcctgcaagcaggttgtgtcacaaagagcactggatccagcatta 540
|| ||| | |||||||||||||||||||| ||||||||||||||||||||||||| |||
Sbjct: 250 attcttttagagagtgcctgcaagcaggtcgtgtcacaaagagcactggatccagaattt 191
Query: 541 ccatcacttccttctttcaccaatcttggaagaagttcatcaaaatacattttccaaatc 600
||||||||||||||||||||||| ||||||| || ||||||||| |||||||||||||||
Sbjct: 190 ccatcacttccttctttcaccaaccttggaataacttcatcaaagtacattttccaaatc 131
Query: 601 tctttgttgatattgatagaatcagcaatgggatgcaaaacccttgggtattgcataggt 660
|||||||||||||| || ||||||||||| ||||||| |||| |||| || || |||||
Sbjct: 130 tctttgttgatattaatggaatcagcaataggatgcagaaccgttggatactggataggc 71
Query: 661 ggcaaccggggctcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtat 720
|||||| | | |||||||||||| | | |||||| |||||||||||||||| |||||||
Sbjct: 70 ggcaacagtgtctcaggcagatcttctggaaagaatgggtgctcatctgggctcttgtat 11
Query: 721 ctcccaacct 730
||||||||||
Sbjct: 10 ctcccaacct 1
>gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011I12f, mRNA sequence
Length = 592
Score = 307 bits (155), Expect = 3e-082
Identities = 288/330 (87%), Gaps = 2/330 (0%)
Strand = Plus / Minus
Query: 424 gtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtggatc 483
||||||||||||||| ||||||||||||||||| |||||||||||||| |||||||||
Sbjct: 499 gtgtaagcttcagggacctcttgaaacttggcctntgccacaaacttcc-atagtggatt 441
Query: 484 -cttctggagagtgcctgcaagcaggttgtgtcacaaagagcactggatccagcattacc 542
||| | |||| |||||||||||||| |||||||||||||||||| ||| || ||| ||
Sbjct: 440 tcttttaaagagggcctgcaagcaggtcgtgtcacaaagagcactgaatcaagaattccc 381
Query: 543 atcacttccttctttcaccaatcttggaagaagttcatcaaaatacattttccaaatctc 602
|||||||| |||||||||||| |||| || || ||||||||| |||||||||||||||||
Sbjct: 380 atcacttctttctttcaccaaccttgaaataacttcatcaaagtacattttccaaatctc 321
Query: 603 tttgttgatattgatagaatcagcaatgggatgcaaaacccttgggtattgcataggtgg 662
|||||||||||| || ||||||||||| ||||||| |||| |||| || || ||||| ||
Sbjct: 320 tttgttgatattaatggaatcagcaataggatgcagaaccgttggatactggataggcgg 261
Query: 663 caaccggggctcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtatct 722
|||| | | |||||||||||| | | |||||| |||||||||||||||| |||||||||
Sbjct: 260 caacagtgtctcaggcagatcttctggaaagaatgggtgctcatctgggctcttgtatct 201
Query: 723 cccaacctgtgcatggagcttttcagtttc 752
|||||||| ||| || ||||||||||||||
Sbjct: 200 cccaacctttgcgtgtagcttttcagtttc 171
>gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023G04f, mRNA sequence
Length = 746
Score = 293 bits (148), Expect = 4e-078
Identities = 232/260 (89%)
Strand = Plus / Minus
Query: 493 agtgcctgcaagcaggttgtgtcacaaagagcactggatccagcattaccatcacttcct 552
||||||||||||||||| ||||||||||||||||||||||||| ||| ||||||||||||
Sbjct: 746 agtgcctgcaagcaggtcgtgtcacaaagagcactggatccagaatttccatcacttcct 687
Query: 553 tctttcaccaatcttggaagaagttcatcaaaatacattttccaaatctctttgttgata 612
||||||||||| ||||||| || ||||||||| |||||||||||||||||||||||||||
Sbjct: 686 tctttcaccaaccttggaataacttcatcaaagtacattttccaaatctctttgttgata 627
Query: 613 ttgatagaatcagcaatgggatgcaaaacccttgggtattgcataggtggcaaccggggc 672
|| || ||||||||||| ||||||| |||| |||| || || ||||| |||||| | | |
Sbjct: 626 ttaatggaatcagcaataggatgcagaaccgttggatactggataggcggcaacagtgtc 567
Query: 673 tcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtatctcccaacctgt 732
||||||||||| | | |||||| |||||||||||||||| ||||||||||||||||| |
Sbjct: 566 tcaggcagatcttctggaaagaatgggtgctcatctgggctcttgtatctcccaaccttt 507
Query: 733 gcatggagcttttcagtttc 752
|| || ||||||||||||||
Sbjct: 506 gcgtgtagcttttcagtttc 487
>gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 3', mRNA sequence
Length = 706
Score = 210 bits (106), Expect = 5e-053
Identities = 203/234 (86%), Gaps = 1/234 (0%)
Strand = Plus / Plus
Query: 301 atgttcacctcttgcccgaagatcttggctttggcctccaccctgtgttcgatagcacgc 360
||||||||||| || || || ||||| || |||| ||||||| |||||||| ||||||
Sbjct: 473 atgttcacctcctgtccaaatatcttagccttggtttccacccgatgttcgatggcacgc 532
Query: 361 tccaccgtttcatatgtgagaaggttcatgagttgatcacggtcttgggctattatggct 420
|| ||||| || || ||||| || | ||| |||||||||| ||| || ||| |||| ||
Sbjct: 533 tcgaccgtctcgtaggtgaggagctgcattagttgatcaccgtcctgagcttttattgca 592
Query: 421 ggcgtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtgg 480
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 593 ggtgtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtgg 652
Query: 481 atccttctggagagtgcctgcaagc-aggttgtgtcacaaagagcactggatcc 533
|| ||| | |||||||||||||||| |||| |||||||||||||||||||||||
Sbjct: 653 attcttttagagagtgcctgcaagcaaggtcgtgtcacaaagagcactggatcc 706
Score = 52.0 bits (26), Expect = 3e-005
Identities = 86/106 (81%)
Strand = Plus / Plus
Query: 167 ctcaatccagcctcctaagcaaatacgcgatttgaacctctttggttagcggcatgattc 226
|||| ||||||||||| ||||| || || | || || || ||||||||||||||||| |
Sbjct: 342 ctcagtccagcctccttagcaagtatgctacctggacgtccttggttagcggcatgatcc 401
Query: 227 tgtagctgtacagctcggcgaccaagctcggaacgatcttgtacac 272
||| ||||||||| | ||||||| ||| || ||||||||||||
Sbjct: 402 tgttgctgtacagtccagcgaccaggctgggcctgatcttgtacac 447
>gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0015C20f, mRNA sequence
Length = 777
Score = 163 bits (82), Expect = 1e-038
Identities = 226/266 (84%), Gaps = 6/266 (2%)
Strand = Plus / Minus
Query: 493 agtgcctgcaagcaggtt-gtgtcacaaaga-gcactggatcca-gcattaccatcactt 549
|||||||||||||||||| ||||| |||||| ||| | ||||| | ||| |||||||||
Sbjct: 771 agtgcctgcaagcaggttcgtgtctcaaagaagcatggaatccaagaatttccatcactt 712
Query: 550 cc-ttctttcaccaatcttgg-aagaagttcatcaaaa-tacattttccaaatctctttg 606
|| || ||||||||| | ||| || || |||||| ||| |||||||||||||||||||||
Sbjct: 711 ccattttttcaccaaccctgggaataacttcatccaaagtacattttccaaatctctttg 652
Query: 607 ttgatattgatagaatcagcaatgggatgcaaaacccttgggtattgcataggtggcaac 666
|||||||| || ||||||||||| ||||||| | || |||| || || ||||| ||||||
Sbjct: 651 ttgatattaatggaatcagcaataggatgcagacccgttggatactggataggcggcaac 592
Query: 667 cggggctcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtatctccca 726
| | |||||||||||| | | |||||| |||||||||||||||| |||||||||||||
Sbjct: 591 agtgtctcaggcagatcttctggaaagaatgggtgctcatctgggctcttgtatctccca 532
Query: 727 acctgtgcatggagcttttcagtttc 752
|||| ||| || ||||||||||||||
Sbjct: 531 acctttgcgtgtagcttttcagtttc 506
>gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0008C14f, mRNA sequence
Length = 660
Score = 131 bits (66), Expect = 4e-029
Identities = 243/302 (80%)
Strand = Plus / Minus
Query: 424 gtgtaagcttcaggggactcttgaaacttggcctctgccacaaacttcccatagtggatc 483
|||||||| ||||||| ||||| | | ||||||| |||| || || |||||||| ||||
Sbjct: 518 gtgtaagcatcagggggctcttaagaattggcctgtgccgcagacgtcccataggggatt 459
Query: 484 cttctggagagtgcctgcaagcaggttgtgtcacaaagagcactggatccagcattacca 543
|| | |||| ||||||||||||| ||||| || | ||||||||||||| |||| ||
Sbjct: 458 gttttatagaggacctgcaagcaggtggtgtcgcacaaagcactggatccaccatttccc 399
Query: 544 tcacttccttctttcaccaatcttggaagaagttcatcaaaatacattttccaaatctct 603
||||||||||||||| |||| ||||||| || ||||||| | |||||||||||||||
Sbjct: 398 tcacttccttctttcgccaaccttggaataacttcatcataggacattttccaaatctgc 339
Query: 604 ttgttgatattgatagaatcagcaatgggatgcaaaacccttgggtattgcataggtggc 663
|||||||||| | ||||||||||| ||||||| |||| || | || ||||| |||
Sbjct: 338 ctgttgatattaagggaatcagcaataggatgcagaaccgctgtatgctggataggcggc 279
Query: 664 aaccggggctcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtatctc 723
|| | | ||||||||||| | | |||||| |||||||||| ||||| ||||||||||
Sbjct: 278 cacagtgtatcaggcagatcttctggaaagaatgggtgctcatatgggctcttgtatctc 219
Query: 724 cc 725
||
Sbjct: 218 cc 217
>gb|BE216096.1|BE216096 HV_CEb0009G14f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0009G14f, mRNA sequence
Length = 578
Score = 89.7 bits (45), Expect = 1e-016
Identities = 72/81 (88%)
Strand = Plus / Minus
Query: 672 ctcaggcagatccttggaaaagaaagggtgctcatctgggcccttgtatctcccaacctg 731
|||||||||||| | | |||||| |||||||||||||||| |||||||||||||||||
Sbjct: 567 ctcaggcagatcttctggaaagaatgggtgctcatctgggctcttgtatctcccaacctt 508
Query: 732 tgcatggagcttttcagtttc 752
||| || ||||||||||||||
Sbjct: 507 tgcgtgtagcttttcagtttc 487
>gb|BI960271.1|BI960271 HVSMEn0024A19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024A19f, mRNA sequence
Length = 633
Score = 77.8 bits (39), Expect = 5e-013
Identities = 57/63 (90%)
Strand = Plus / Minus
Query: 690 aaagaaagggtgctcatctgggcccttgtatctcccaacctgtgcatggagcttttcagt 749
|||||| |||||||||| ||||| ||||||||||||||||| ||| || |||||||||||
Sbjct: 628 aaagaatgggtgctcatttgggctcttgtatctcccaacctttgcgtgtagcttttcagt 569
Query: 750 ttc 752
|||
Sbjct: 568 ttc 566
>gb|AV921077.1|AV921077 AV921077 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16n12 3', mRNA sequence
Length = 428
Score = 52.0 bits (26), Expect = 3e-005
Identities = 86/106 (81%)
Strand = Plus / Plus
Query: 167 ctcaatccagcctcctaagcaaatacgcgatttgaacctctttggttagcggcatgattc 226
|||| ||||||||||| ||||| || || | || || || ||||||||||||||||| |
Sbjct: 283 ctcagtccagcctccttagcaagtatgctacctggacgtccttggttagcggcatgatcc 342
Query: 227 tgtagctgtacagctcggcgaccaagctcggaacgatcttgtacac 272
||| ||||||||| | ||||||| ||| || ||||||||||||
Sbjct: 343 tgttgctgtacagtccagcgaccaggctgggcctgatcttgtacac 388
>gb|BU995988.1|BU995988 HM12A03r HM Hordeum vulgare subsp. vulgare cDNA clone HM12A03
5-PRIME, mRNA sequence
Length = 589
Score = 52.0 bits (26), Expect = 3e-005
Identities = 86/106 (81%)
Strand = Plus / Minus
Query: 167 ctcaatccagcctcctaagcaaatacgcgatttgaacctctttggttagcggcatgattc 226
|||| ||||||||||| ||||| || || | || || || ||||||||||||||||| |
Sbjct: 243 ctcagtccagcctccttagcaagtatgctacctggacgtccttggttagcggcatgatcc 184
Query: 227 tgtagctgtacagctcggcgaccaagctcggaacgatcttgtacac 272
||| ||||||||| | ||||||| ||| || ||||||||||||
Sbjct: 183 tgttgctgtacagtccagcgaccaggctgggcctgatcttgtacac 138
Score = 48.1 bits (24), Expect = 4e-004
Identities = 81/100 (81%)
Strand = Plus / Minus
Query: 301 atgttcacctcttgcccgaagatcttggctttggcctccaccctgtgttcgatagcacgc 360
||||||||||| || || || ||||| || |||| ||||||| |||||||| ||||||
Sbjct: 112 atgttcacctcctgtccaaatatcttagccttggtttccacccgatgttcgatggcacgc 53
Query: 361 tccaccgtttcatatgtgagaaggttcatgagttgatcac 400
|| ||||| || || ||||| || | ||| ||||||||||
Sbjct: 52 tcgaccgtctcgtaggtgaggagctgcattagttgatcac 13
>gb|BE455736.3|BE455736 HVSMEg0019G20f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0019G20f, mRNA sequence
Length = 829
Score = 46.1 bits (23), Expect = 0.002
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 709 gggcccttgtatctcccaacctgtgcatggagcttttcagttt 751
|||| ||||||||||||| ||| ||| || |||||||||||||
Sbjct: 531 gggctcttgtatctcccaccctttgcgtgtagcttttcagttt 489
>gb|BI952463.1|BI952463 HVSMEm0006I19f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0006I19f, mRNA sequence
Length = 871
Score = 40.1 bits (20), Expect = 0.10
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 521 gagcactggatccagcattaccat 544
|||||||| |||||||||||||||
Sbjct: 441 gagcactgaatccagcattaccat 418
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 62,453
Number of Sequences: 312970
Number of extensions: 62453
Number of successful extensions: 15454
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15427
Number of HSP's gapped (non-prelim): 21
length of query: 760
length of database: 175,134,539
effective HSP length: 19
effective length of query: 741
effective length of database: 169,188,109
effective search space: 125368388769
effective search space used: 125368388769
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)