BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493528.2.1
(627 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV834576.1|AV834576 AV834576 K. Sato unpublished cDNA li... 456 e-127
gb|CA005317.1|CA005317 HU11A16u HU Hordeum vulgare subsp. v... 343 4e-093
gb|BM100585.1|BM100585 EBma01_SQ005_K12_R maternal, 4 DPA, ... 274 3e-072
gb|AJ435202.1|AJ435202 AJ435202 S00002 Hordeum vulgare subs... 226 7e-058
gb|BQ468117.1|BQ468117 HR01P23r HR Hordeum vulgare subsp. v... 38 0.34
gb|BQ662195.1|BQ662195 HR01P23u HR Hordeum vulgare subsp. v... 38 0.34
gb|BQ757450.1|BQ757450 EBem10_SQ004_C16_R embryo, 2 Day ger... 38 0.34
>gb|AV834576.1|AV834576 AV834576 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags3i09, mRNA sequence
Length = 686
Score = 456 bits (230), Expect = e-127
Identities = 467/548 (85%)
Strand = Plus / Plus
Query: 54 tcatccagctaattgactaacacttgagctcagtaaagtattgatctctccaattatata 113
||||||||||||||||||| ||||||| ||||||||| |||| ||||||||||||||||
Sbjct: 74 tcatccagctaattgactaccacttgaactcagtaaattattaatctctccaattatatg 133
Query: 114 cactggtcttccatcaaaaacagacgacagtgtcgactcacacagcttctttagttccct 173
|||||| || | ||| || | ||| || ||||| | |||||| |||||||||||||||||
Sbjct: 134 cactggcctgctatcgaacatagatgaaagtgttgcctcacagagcttctttagttccct 193
Query: 174 tgtttttgtaattgctgcctcttgctttggccatgatgatgacagtgcgttttgcagctc 233
||||||||||| |||||||||||||| ||||| ||||| |||| |||||||||||
Sbjct: 194 cgtttttgtaatcgctgcctcttgcttcggccaggatgactttngtgcattttgcagctc 253
Query: 234 catacgcttaacagataaatcacctaaaccttcccgtagttttacttcgtgatgcttctt 293
||| ||||| || || |||| |||||| ||||| ||| || || ||||| |||||
Sbjct: 254 catgcgcttcactgacaaatnacctaagccttctcgtnccnncacctcatgatgtttctt 313
Query: 294 ctcttctaagcttgaaactaatctatccagaaaccttttagagttaaggatcattataag 353
||||||||| |||| || ||||| |||| ||| |||| |||||| ||||||||||||||
Sbjct: 314 ttcttctaaggttgacaccaatctgtccaaaaatctttnagagttgaggatcattataag 373
Query: 354 gtcaagagtcttctgattagttagcaatgagattgctgacgagatctctacaagcatgtt 413
||||||||| ||||| ||||| |||| ||||||||||| || | |||||||||||| ||
Sbjct: 374 gtcaagagttttctggttagtgagcagagagattgctgaggaaacctctacaagcatatt 433
Query: 414 ctccaaactgtcaggagcatgctgctggagaacaaaaggtgagacagcttgcacttgatg 473
|||||||| |||||||||| ||| |||||||||||||||||||| ||||| || |||||
Sbjct: 434 ctccaaacgatcaggagcatactgttggagaacaaaaggtgagacggcttggacctgatg 493
Query: 474 ctgtagagatgatgtgtctgcattcctcatctcccctaagcgttgagtcaaaaatgattt 533
||||||||||||||| || ||||| ||||||||| ||| |||| | ||||||||||||||
Sbjct: 494 ctgtagagatgatgtatcagcatttctcatctccgctaggcgtagggtcaaaaatgattt 553
Query: 534 tacctcgagtaaatcatccagaatattattcctatattctttctccagcaacaaacttct 593
||||| ||||||||||||||||||||||| |||||||| |||||||||||| ||||||
Sbjct: 554 cacctcaagtaaatcatccagaatattatttctatattccttctccagcaactgacttct 613
Query: 594 atcttcag 601
|||||||
Sbjct: 614 ttcttcag 621
>gb|CA005317.1|CA005317 HU11A16u HU Hordeum vulgare subsp. vulgare cDNA clone HU11A16
3-PRIME, mRNA sequence
Length = 478
Score = 343 bits (173), Expect = 4e-093
Identities = 338/393 (86%)
Strand = Plus / Plus
Query: 54 tcatccagctaattgactaacacttgagctcagtaaagtattgatctctccaattatata 113
||||||||||||||||||| ||||||| ||||||||| |||| ||||||||||||||||
Sbjct: 86 tcatccagctaattgactaccacttgaactcagtaaattattaatctctccaattatatg 145
Query: 114 cactggtcttccatcaaaaacagacgacagtgtcgactcacacagcttctttagttccct 173
|||||| || | ||| || | ||| || ||||| | |||||| |||||||||||||||||
Sbjct: 146 cactggcctgctatcgaacatagatgaaagtgttgcctcacagagcttctttagttccct 205
Query: 174 tgtttttgtaattgctgcctcttgctttggccatgatgatgacagtgcgttttgcagctc 233
||||||||||| |||||||||||||| ||||| ||||| || ||||| |||||||||||
Sbjct: 206 cgtttttgtaatcgctgcctcttgcttcggccaggatgacgatagtgcattttgcagctc 265
Query: 234 catacgcttaacagataaatcacctaaaccttcccgtagttttacttcgtgatgcttctt 293
||| ||||| || || ||||||||||| ||||| ||||| || || || ||||| |||||
Sbjct: 266 catgcgcttcactgacaaatcacctaagccttctcgtagcttcacctcatgatgtttctt 325
Query: 294 ctcttctaagcttgaaactaatctatccagaaaccttttagagttaaggatcattataag 353
||||||||| |||| || ||||| |||| ||| ||||||||||| ||||||||||||||
Sbjct: 326 ttcttctaaggttgacaccaatctgtccaaaaatcttttagagttgaggatcattataag 385
Query: 354 gtcaagagtcttctgattagttagcaatgagattgctgacgagatctctacaagcatgtt 413
||||||||| ||||| ||||| |||| ||||||||||| || | |||||||||||| ||
Sbjct: 386 gtcaagagttttctggttagtgagcagagagattgctgaggaaacctctacaagcatatt 445
Query: 414 ctccaaactgtcaggagcatgctgctggagaac 446
|||||||| |||||||||| ||| ||||||||
Sbjct: 446 ctccaaacgatcaggagcatactgttggagaac 478
>gb|BM100585.1|BM100585 EBma01_SQ005_K12_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_K12 5', mRNA sequence
Length = 429
Score = 274 bits (138), Expect = 3e-072
Identities = 264/306 (86%)
Strand = Plus / Minus
Query: 54 tcatccagctaattgactaacacttgagctcagtaaagtattgatctctccaattatata 113
||||||||||||||||||| ||||||| ||||||||| |||| ||||||||||||||||
Sbjct: 306 tcatccagctaattgactaccacttgaactcagtaaattattaatctctccaattatatg 247
Query: 114 cactggtcttccatcaaaaacagacgacagtgtcgactcacacagcttctttagttccct 173
|||||| || | ||| || | ||| || ||||| | |||||| |||||||||||||||||
Sbjct: 246 cactggcctgctatcgaacatagatgaaagtgttgcctcacagagcttctttagttccct 187
Query: 174 tgtttttgtaattgctgcctcttgctttggccatgatgatgacagtgcgttttgcagctc 233
||||||||||| |||||||||||||| ||||| ||||| || ||||| |||||||||||
Sbjct: 186 cgtttttgtaatcgctgcctcttgcttcggccaggatgacgatagtgcattttgcagctc 127
Query: 234 catacgcttaacagataaatcacctaaaccttcccgtagttttacttcgtgatgcttctt 293
||| ||||| || || ||||||||||| ||||| ||||| || || || ||||| |||||
Sbjct: 126 catgcgcttcactgacaaatcacctaagccttctcgtagcttcacctcatgatgtttctt 67
Query: 294 ctcttctaagcttgaaactaatctatccagaaaccttttagagttaaggatcattataag 353
||||||||| |||| || ||||| |||| ||| ||||||||||| ||||||||||||||
Sbjct: 66 ttcttctaaggttgacaccaatctgtccaaaaatcttttagagttgaggatcattataag 7
Query: 354 gtcaag 359
||||||
Sbjct: 6 gtcaag 1
>gb|AJ435202.1|AJ435202 AJ435202 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200048C01F1, mRNA sequence
Length = 533
Score = 226 bits (114), Expect = 7e-058
Identities = 237/278 (85%)
Strand = Plus / Minus
Query: 54 tcatccagctaattgactaacacttgagctcagtaaagtattgatctctccaattatata 113
||||||||||||||||||| ||||||| ||||||||| |||| ||||||||||||||||
Sbjct: 452 tcatccagctaattgactaccacttgaactcagtaaattattaatctctccaattatatg 393
Query: 114 cactggtcttccatcaaaaacagacgacagtgtcgactcacacagcttctttagttccct 173
|||||| || | ||| || | ||| || ||||| | |||||| |||||||||||||||||
Sbjct: 392 cactggcctgctatcgaacatagatgaaagtgttgcctcacagagcttctttagttccct 333
Query: 174 tgtttttgtaattgctgcctcttgctttggccatgatgatgacagtgcgttttgcagctc 233
||||||||||| |||||||||||||| ||||| ||||| || ||||| |||||||||||
Sbjct: 332 cgtttttgtaatcgctgcctcttgcttcggccaggatgacgatagtgcattttgcagctc 273
Query: 234 catacgcttaacagataaatcacctaaaccttcccgtagttttacttcgtgatgcttctt 293
||| ||||| || || ||||||||||| ||||| ||||| || || || ||||| |||||
Sbjct: 272 catgcgcttcactgacaaatcacctaagccttctcgtagcttcacctcatgatgtttctt 213
Query: 294 ctcttctaagcttgaaactaatctatccagaaaccttt 331
||||||||| |||| || ||||| |||| ||| ||||
Sbjct: 212 ttcttctaaggttgacaccaatctgtccaaaaatcttt 175
>gb|BQ468117.1|BQ468117 HR01P23r HR Hordeum vulgare subsp. vulgare cDNA clone HR01P23
5-PRIME, mRNA sequence
Length = 383
Score = 38.2 bits (19), Expect = 0.34
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 15 atttttgcaatcttatttg 33
|||||||||||||||||||
Sbjct: 373 atttttgcaatcttatttg 355
>gb|BQ662195.1|BQ662195 HR01P23u HR Hordeum vulgare subsp. vulgare cDNA clone HR01P23
3-PRIME, mRNA sequence
Length = 383
Score = 38.2 bits (19), Expect = 0.34
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 15 atttttgcaatcttatttg 33
|||||||||||||||||||
Sbjct: 11 atttttgcaatcttatttg 29
>gb|BQ757450.1|BQ757450 EBem10_SQ004_C16_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ004_C16 5', mRNA sequence
Length = 325
Score = 38.2 bits (19), Expect = 0.34
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 15 atttttgcaatcttatttg 33
|||||||||||||||||||
Sbjct: 196 atttttgcaatcttatttg 178
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 53,767
Number of Sequences: 312970
Number of extensions: 53767
Number of successful extensions: 14329
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 14320
Number of HSP's gapped (non-prelim): 8
length of query: 627
length of database: 175,134,539
effective HSP length: 19
effective length of query: 608
effective length of database: 169,188,109
effective search space: 102866370272
effective search space used: 102866370272
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)