BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493499.2.1
(785 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. v... 70 1e-010
gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedl... 52 3e-005
gb|BM816841.1|BM816841 HB105A11_SK.ab1 HB Hordeum vulgare s... 52 3e-005
gb|CA010560.1|CA010560 HT09K12u HT Hordeum vulgare subsp. v... 52 3e-005
gb|CA016572.1|CA016572 HV06P19u HV Hordeum vulgare subsp. v... 52 3e-005
gb|CA016687.1|CA016687 HV06O20u HV Hordeum vulgare subsp. v... 52 3e-005
gb|CA016852.1|CA016852 HV06B13u HV Hordeum vulgare subsp. v... 52 3e-005
gb|BF620291.2|BF620291 HVSMEc0019F18f Hordeum vulgare seedl... 50 1e-004
gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green... 48 4e-004
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 46 0.002
gb|CB879235.1|CB879235 HP11G23T HP Hordeum vulgare subsp. v... 46 0.002
gb|BM444331.1|BM444331 EBem09_SQ004_N12_R embryo, 1 Day ger... 42 0.027
gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedl... 40 0.11
gb|BF260368.2|BF260368 HVSMEf0021M24f Hordeum vulgare seedl... 40 0.11
gb|BI956610.1|BI956610 HVSMEn0004G06f Hordeum vulgare rachi... 40 0.11
gb|BF262509.1|BF262509 HV_CEa0004C17f Hordeum vulgare seedl... 40 0.11
gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. v... 40 0.11
gb|BM372537.2|BM372537 EBpi05_SQ003_D24_R pistil, 8 DPA, no... 40 0.11
gb|CB882618.1|CB882618 HL02G02w HL Hordeum vulgare subsp. v... 40 0.11
gb|BI952404.1|BI952404 HVSMEm0006E21f Hordeum vulgare green... 38 0.42
gb|BF267582.3|BF267582 HV_CEa0018F10f Hordeum vulgare seedl... 38 0.42
gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subs... 38 0.42
gb|BU994136.1|BU994136 HM05N21r HM Hordeum vulgare subsp. v... 38 0.42
gb|BU996279.1|BU996279 HM13B16r HM Hordeum vulgare subsp. v... 38 0.42
gb|CB880799.1|CB880799 HM07N21w HM Hordeum vulgare subsp. v... 38 0.42
>gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU13D11
5-PRIME, mRNA sequence
Length = 581
Score = 69.9 bits (35), Expect = 1e-010
Identities = 44/47 (93%)
Strand = Plus / Minus
Query: 453 ccacccgcagtgcgtccagaacccacccaccgccgggtgcgccagga 499
|||||||||||||||| |||||| ||||||||||||||||||||||
Sbjct: 352 ccacccgcagtgcgtcatgaacccgcccaccgccgggtgcgccagga 306
>gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017B13f, mRNA sequence
Length = 875
Score = 52.0 bits (26), Expect = 3e-005
Identities = 59/70 (84%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccaccgccgggtgcgccaggacctccctctgc 510
|||||||||||||||||| |||||| |||||||| ||||||||| || || | ||||
Sbjct: 319 ttccacccgcagtgcgtcacgaacccgcccaccgcgccgtgcgccagcacgtcgcgctgc 260
Query: 511 ggtgcccacg 520
|| |||||||
Sbjct: 259 ggcgcccacg 250
>gb|BM816841.1|BM816841 HB105A11_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB105A11_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 872
Score = 52.0 bits (26), Expect = 3e-005
Identities = 44/50 (88%)
Strand = Plus / Minus
Query: 437 cctccaccatggatttccacccgcagtgcgtccagaacccacccaccgcc 486
|||||| |||||| |||||||||||||||||| |||| | |||||||||
Sbjct: 558 cctccatcatggagttccacccgcagtgcgtcaagaaggcgcccaccgcc 509
>gb|CA010560.1|CA010560 HT09K12u HT Hordeum vulgare subsp. vulgare cDNA clone HT09K12
3-PRIME, mRNA sequence
Length = 361
Score = 52.0 bits (26), Expect = 3e-005
Identities = 44/50 (88%)
Strand = Plus / Plus
Query: 437 cctccaccatggatttccacccgcagtgcgtccagaacccacccaccgcc 486
|||||| |||||| |||||||||||||||||| |||| | |||||||||
Sbjct: 298 cctccatcatggagttccacccgcagtgcgtcaagaaggcgcccaccgcc 347
>gb|CA016572.1|CA016572 HV06P19u HV Hordeum vulgare subsp. vulgare cDNA clone HV06P19
3-PRIME, mRNA sequence
Length = 447
Score = 52.0 bits (26), Expect = 3e-005
Identities = 44/50 (88%)
Strand = Plus / Plus
Query: 437 cctccaccatggatttccacccgcagtgcgtccagaacccacccaccgcc 486
|||||| |||||| |||||||||||||||||| |||| | |||||||||
Sbjct: 321 cctccatcatggagttccacccgcagtgcgtcaagaaggcgcccaccgcc 370
>gb|CA016687.1|CA016687 HV06O20u HV Hordeum vulgare subsp. vulgare cDNA clone HV06O20
3-PRIME, mRNA sequence
Length = 490
Score = 52.0 bits (26), Expect = 3e-005
Identities = 44/50 (88%)
Strand = Plus / Plus
Query: 437 cctccaccatggatttccacccgcagtgcgtccagaacccacccaccgcc 486
|||||| |||||| |||||||||||||||||| |||| | |||||||||
Sbjct: 321 cctccatcatggagttccacccgcagtgcgtcaagaaggcgcccaccgcc 370
>gb|CA016852.1|CA016852 HV06B13u HV Hordeum vulgare subsp. vulgare cDNA clone HV06B13
3-PRIME, mRNA sequence
Length = 365
Score = 52.0 bits (26), Expect = 3e-005
Identities = 44/50 (88%)
Strand = Plus / Plus
Query: 437 cctccaccatggatttccacccgcagtgcgtccagaacccacccaccgcc 486
|||||| |||||| |||||||||||||||||| |||| | |||||||||
Sbjct: 304 cctccatcatggagttccacccgcagtgcgtcaagaaggcgcccaccgcc 353
>gb|BF620291.2|BF620291 HVSMEc0019F18f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0019F18f, mRNA sequence
Length = 689
Score = 50.1 bits (25), Expect = 1e-004
Identities = 58/69 (84%)
Strand = Plus / Minus
Query: 450 tttccacccgcagtgcgtccagaacccacccaccgccgggtgcgccaggacctccctctg 509
||||||||| | ||||||||||||||| |||| |||||||||| || |||||| |||
Sbjct: 664 tttccaccccccgtgcgtccagaacccggccacggccgggtgcggaagcacctcctcctg 605
Query: 510 cggtgccca 518
||| |||||
Sbjct: 604 cggcgccca 596
Score = 40.1 bits (20), Expect = 0.11
Identities = 41/48 (85%)
Strand = Plus / Minus
Query: 625 acccacaggaacggcacgccgctgccggccagcgcccacgccgtctcc 672
||||| ||||||||||||| |||||| || | ||||||||||||||
Sbjct: 504 acccagaggaacggcacgcggctgccagcgatgccccacgccgtctcc 457
>gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012J18f, mRNA sequence
Length = 855
Score = 48.1 bits (24), Expect = 4e-004
Identities = 33/36 (91%)
Strand = Plus / Minus
Query: 484 gccgggtgcgccaggacctccctctgcggtgcccac 519
|||||||||||||||| |||| |||||||||||||
Sbjct: 275 gccgggtgcgccaggatctccagctgcggtgcccac 240
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 46.1 bits (23), Expect = 0.002
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccaccgccgggtgcg 493
||||| |||||||||||| ||| || ||||||||||||||||
Sbjct: 322 ttccagccgcagtgcgtcaggaagccgcccaccgccgggtgcg 280
>gb|CB879235.1|CB879235 HP11G23T HP Hordeum vulgare subsp. vulgare cDNA clone HP11G23
5-PRIME, mRNA sequence
Length = 622
Score = 46.1 bits (23), Expect = 0.002
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccaccgccgggtgcg 493
||||| |||||||||||| ||| || ||||||||||||||||
Sbjct: 70 ttccagccgcagtgcgtcaggaagccgcccaccgccgggtgcg 28
>gb|BM444331.1|BM444331 EBem09_SQ004_N12_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_N12 5', mRNA sequence
Length = 340
Score = 42.1 bits (21), Expect = 0.027
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 436 gcctccaccatggatttccacccgcagtgcgtc 468
||||||| | |||| ||||||||||||||||||
Sbjct: 166 gcctccagcgtggagttccacccgcagtgcgtc 134
>gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012H19f, mRNA sequence
Length = 847
Score = 40.1 bits (20), Expect = 0.11
Identities = 41/48 (85%)
Strand = Plus / Minus
Query: 625 acccacaggaacggcacgccgctgccggccagcgcccacgccgtctcc 672
||||| ||||||||||||| |||||| || | ||||||||||||||
Sbjct: 304 acccagaggaacggcacgcggctgccagcgatgccccacgccgtctcc 257
Score = 38.2 bits (19), Expect = 0.42
Identities = 28/31 (90%)
Strand = Plus / Minus
Query: 462 gtgcgtccagaacccacccaccgccgggtgc 492
||||||||||||||| |||| |||||||||
Sbjct: 452 gtgcgtccagaacccggccacggccgggtgc 422
>gb|BF260368.2|BF260368 HVSMEf0021M24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021M24f, mRNA sequence
Length = 816
Score = 40.1 bits (20), Expect = 0.11
Identities = 44/52 (84%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccaccgccgggtgcgccaggacct 502
||||| ||||||||||||| ||| | |||||||| ||||||| ||| ||||
Sbjct: 151 ttccaaccgcagtgcgtcccgaagcaccccaccgcagggtgcgacagcacct 100
>gb|BI956610.1|BI956610 HVSMEn0004G06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0004G06f, mRNA sequence
Length = 898
Score = 40.1 bits (20), Expect = 0.11
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 402 cggctgcacgagcatgggca 421
||||||||||||||||||||
Sbjct: 534 cggctgcacgagcatgggca 515
>gb|BF262509.1|BF262509 HV_CEa0004C17f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0004C17f, mRNA sequence
Length = 873
Score = 40.1 bits (20), Expect = 0.11
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccac 482
|||||||||||||||||| ||| ||||||||
Sbjct: 78 ttccacccgcagtgcgtcaggaagccacccac 47
>gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. vulgare cDNA clone HR01O04
3-PRIME, mRNA sequence
Length = 532
Score = 40.1 bits (20), Expect = 0.11
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 433 atggcctccaccatggatttccacccgcagtg 464
|||||||||| | |||| ||||||||||||||
Sbjct: 428 atggcctccagcgtggagttccacccgcagtg 459
>gb|BM372537.2|BM372537 EBpi05_SQ003_D24_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_D24 5', mRNA sequence
Length = 507
Score = 40.1 bits (20), Expect = 0.11
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 725 cggagcgcggcgggcgcgcg 744
||||||||||||||||||||
Sbjct: 470 cggagcgcggcgggcgcgcg 451
>gb|CB882618.1|CB882618 HL02G02w HL Hordeum vulgare subsp. vulgare cDNA clone HL02G02
3-PRIME, mRNA sequence
Length = 619
Score = 40.1 bits (20), Expect = 0.11
Identities = 44/52 (84%)
Strand = Plus / Minus
Query: 451 ttccacccgcagtgcgtccagaacccacccaccgccgggtgcgccaggacct 502
||||| |||||||||||| ||| | |||||||||||||||| ||| ||||
Sbjct: 590 ttccaaccgcagtgcgtcacgaagcaccccaccgccgggtgcgacagcacct 539
>gb|BI952404.1|BI952404 HVSMEm0006E21f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0006E21f, mRNA sequence
Length = 835
Score = 38.2 bits (19), Expect = 0.42
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 578 catcctcctcatccgcgtc 596
|||||||||||||||||||
Sbjct: 121 catcctcctcatccgcgtc 139
>gb|BF267582.3|BF267582 HV_CEa0018F10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0018F10f, mRNA sequence
Length = 602
Score = 38.2 bits (19), Expect = 0.42
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 446 tggatttccacccgcagtgcgtc 468
|||| ||||||||||||||||||
Sbjct: 52 tggagttccacccgcagtgcgtc 30
>gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058E12F1, mRNA sequence
Length = 360
Score = 38.2 bits (19), Expect = 0.42
Identities = 28/31 (90%)
Strand = Plus / Minus
Query: 462 gtgcgtccagaacccacccaccgccgggtgc 492
||||||||||||||| |||| |||||||||
Sbjct: 85 gtgcgtccagaacccggccacggccgggtgc 55
>gb|BU994136.1|BU994136 HM05N21r HM Hordeum vulgare subsp. vulgare cDNA clone HM05N21
5-PRIME, mRNA sequence
Length = 554
Score = 38.2 bits (19), Expect = 0.42
Identities = 37/43 (86%)
Strand = Plus / Minus
Query: 457 ccgcagtgcgtccagaacccacccaccgccgggtgcgccagga 499
|||||||||||| ||| || ||||| |||||||||| |||||
Sbjct: 144 ccgcagtgcgtcatgaagccccccacggccgggtgcgacagga 102
>gb|BU996279.1|BU996279 HM13B16r HM Hordeum vulgare subsp. vulgare cDNA clone HM13B16
5-PRIME, mRNA sequence
Length = 507
Score = 38.2 bits (19), Expect = 0.42
Identities = 34/39 (87%)
Strand = Plus / Minus
Query: 724 acggagcgcggcgggcgcgcgtccagccacgccaagcag 762
||||||||||||| |||||||||||||| ||| ||||
Sbjct: 379 acggagcgcggcgcctgcgcgtccagccaccccacgcag 341
>gb|CB880799.1|CB880799 HM07N21w HM Hordeum vulgare subsp. vulgare cDNA clone HM07N21
3-PRIME, mRNA sequence
Length = 550
Score = 38.2 bits (19), Expect = 0.42
Identities = 37/43 (86%)
Strand = Plus / Plus
Query: 457 ccgcagtgcgtccagaacccacccaccgccgggtgcgccagga 499
|||||||||||| ||| || ||||| |||||||||| |||||
Sbjct: 411 ccgcagtgcgtcatgaagccccccacggccgggtgcgacagga 453
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 109,994
Number of Sequences: 312970
Number of extensions: 109994
Number of successful extensions: 32631
Number of sequences better than 0.5: 25
Number of HSP's better than 0.5 without gapping: 25
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 32582
Number of HSP's gapped (non-prelim): 47
length of query: 785
length of database: 175,134,539
effective HSP length: 19
effective length of query: 766
effective length of database: 169,188,109
effective search space: 129598091494
effective search space used: 129598091494
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)