BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2485944.3.1
(991 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ447037.1|BJ447037 BJ447037 K. Sato unpublished cDNA li... 274 5e-072
gb|BJ449238.1|BJ449238 BJ449238 K. Sato unpublished cDNA li... 274 5e-072
gb|AV926512.1|AV926512 AV926512 K. Sato unpublished cDNA li... 228 3e-058
gb|CA025182.1|CA025182 HZ51H02r HZ Hordeum vulgare subsp. v... 174 4e-042
gb|CA025064.1|CA025064 HZ51B21r HZ Hordeum vulgare subsp. v... 145 3e-033
gb|AL506332.1|AL506332 AL506332 Hordeum vulgare Barke devel... 119 2e-025
gb|AV931399.1|AV931399 AV931399 K. Sato unpublished cDNA li... 113 1e-023
gb|BI956152.1|BI956152 HVSMEm0025N07f Hordeum vulgare green... 105 3e-021
gb|BJ456704.1|BJ456704 BJ456704 K. Sato unpublished cDNA li... 86 3e-015
gb|BQ467644.1|BQ467644 HS04G20r HS Hordeum vulgare subsp. v... 50 1e-004
gb|CA004275.1|CA004275 HS17B20r HS Hordeum vulgare subsp. v... 50 1e-004
gb|CA012143.1|CA012143 HT04J04r HT Hordeum vulgare subsp. v... 50 1e-004
>gb|BJ447037.1|BJ447037 BJ447037 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak15m16 5', mRNA sequence
Length = 656
Score = 274 bits (138), Expect = 5e-072
Identities = 369/446 (82%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 93 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 152
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | ||| || |||||||| ||||| |||||||| |||
Sbjct: 153 ggcttcaagctgggtgaccatgtggctgtggggacatatgtcaactcatgccgtgactgt 212
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
|| |||||||||| || || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 213 gacaactgcaatagcttcctcgagaaccactgctcaaaatttgttttcactttcaatggt 272
Query: 723 attgatacagatggaactgtcacaaaggggggttactccactcacattgtagtccatgaa 782
||||||| ||||| |||||||||||||| || || |||| |||||||||||| ||||||
Sbjct: 273 gttgatacggatggtactgtcacaaagggaggatattccagtcacattgtagttcatgaa 332
Query: 783 aggtattgcttcaaaatacccgatggctaccctttggcgaaggcagcacctcttctgtgt 842
|||| |||| |||||||| ||||||||||| ||| ||||| |||||| | | |||
Sbjct: 333 cggtactgctataaaatacctgatggctacccactggaaaaggccgcacctttagtttgt 392
Query: 843 gctggaataaccgtgtatactccaatgatgcgacacaacatgaaccaacctggaaagtca 902
|||||||| || ||||| ||||| |||| |||||| ||||||||||| || || ||||||
Sbjct: 393 gctggaatcactgtgtacactccgatgacgcgacataacatgaaccagccagggaagtca 452
Query: 903 cttggggtcattggtcttggtggtctgggtcacatggctgtgaaatttggtaaagcattt 962
||||| || | ||||||||||| ||||||||||||| ||||||||||| ||||| |||
Sbjct: 453 cttggtgttgtcggtcttggtgggttgggtcacatggcagtgaaatttgggaaagccttt 512
Query: 963 ggtcttaaggtcacagttttcagtac 988
|| || ||||| || ||||| |||||
Sbjct: 513 ggactgaaggtgactgttttgagtac 538
>gb|BJ449238.1|BJ449238 BJ449238 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak23a07 5', mRNA sequence
Length = 573
Score = 274 bits (138), Expect = 5e-072
Identities = 369/446 (82%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 83 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 142
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | ||| || |||||||| ||||| |||||||| |||
Sbjct: 143 ggcttcaagctgggtgaccatgtggctgtggggacatatgtcaactcatgccgtgactgt 202
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
|| |||||||||| || || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 203 gacaactgcaatagcttcctcgagaaccactgctcaaaatttgttttcactttcaatggt 262
Query: 723 attgatacagatggaactgtcacaaaggggggttactccactcacattgtagtccatgaa 782
||||||| ||||| |||||||||||||| || || |||| |||||||||||| ||||||
Sbjct: 263 gttgatacggatggtactgtcacaaagggaggatattccagtcacattgtagttcatgaa 322
Query: 783 aggtattgcttcaaaatacccgatggctaccctttggcgaaggcagcacctcttctgtgt 842
|||| |||| |||||||| ||||||||||| ||| ||||| |||||| | | |||
Sbjct: 323 cggtactgctataaaatacctgatggctacccactggaaaaggccgcacctttagtttgt 382
Query: 843 gctggaataaccgtgtatactccaatgatgcgacacaacatgaaccaacctggaaagtca 902
|||||||| || ||||| ||||| |||| |||||| ||||||||||| || || ||||||
Sbjct: 383 gctggaatcactgtgtacactccgatgacgcgacataacatgaaccagccagggaagtca 442
Query: 903 cttggggtcattggtcttggtggtctgggtcacatggctgtgaaatttggtaaagcattt 962
||||| || | ||||||||||| ||||||||||||| ||||||||||| ||||| |||
Sbjct: 443 cttggtgttgtcggtcttggtgggttgggtcacatggcagtgaaatttgggaaagccttt 502
Query: 963 ggtcttaaggtcacagttttcagtac 988
|| || ||||| || ||||| |||||
Sbjct: 503 ggactgaaggtgactgttttgagtac 528
>gb|AV926512.1|AV926512 AV926512 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19k10 5', mRNA sequence
Length = 658
Score = 228 bits (115), Expect = 3e-058
Identities = 289/347 (83%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 311 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 370
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || |||| |||||||| | |||||| |||||||| ||||| |||||||| |||
Sbjct: 371 ggcttcaagctgggcgaccatgtggctgttgggacatatgtcaactcatgccgtgactgt 430
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
||||||||||||| ||| || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 431 gagaactgcaatagctccctcgagaaccactgctcaaaatttgttttcactttcaatggt 490
Query: 723 attgatacagatggaactgtcacaaaggggggttactccactcacattgtagtccatgaa 782
||||||| ||||| |||||||||||||| || || |||| |||||||||||| ||||||
Sbjct: 491 gttgatacggatggtactgtcacaaagggaggatattccagtcacattgtagttcatgaa 550
Query: 783 aggtattgcttcaaaatacccgatggctaccctttggcgaaggcagcacctcttctgtgt 842
|||| |||| |||||||| ||||||||||| ||| ||||| |||||| | | |||
Sbjct: 551 cggtactgctataaaatacctgatggctacccactggaaaaggccgcacctttagtttgt 610
Query: 843 gctggaataaccgtgtatactccaatgatgcgacacaacatgaacca 889
|||||||| || ||||| ||||| |||| |||||| |||||||||||
Sbjct: 611 gctggaatcactgtgtacactccgatgacgcgacataacatgaacca 657
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 143 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctcccctcacactttc 202
Query: 435 aaccg 439
|||||
Sbjct: 203 aaccg 207
>gb|CA025182.1|CA025182 HZ51H02r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51H02
5-PRIME, mRNA sequence
Length = 603
Score = 174 bits (88), Expect = 4e-042
Identities = 226/272 (83%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 328 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 387
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | ||| || |||||||| ||||| |||||||| |||
Sbjct: 388 ggcttcaagctgggtgaccatgtggctgtggggacatatgtcaactcatgccgtgactgt 447
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
|| |||||||||| || || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 448 gacaactgcaatagcttcctcgagaaccactgctcaaaatttgttttcactttcaatggt 507
Query: 723 attgatacagatggaactgtcacaaaggggggttactccactcacattgtagtccatgaa 782
||||||| ||||| |||||||||||||| || || |||| |||| ||||||| ||||||
Sbjct: 508 gttgatacggatggtactgtcacaaagggaggatattccagtcacgttgtagttcatgaa 567
Query: 783 aggtattgcttcaaaatacccgatggctaccc 814
|||| |||| |||||||| |||||||||||
Sbjct: 568 cggtactgctataaaatacctgatggctaccc 599
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 160 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 219
Query: 435 aaccg 439
|||||
Sbjct: 220 aaccg 224
>gb|CA025064.1|CA025064 HZ51B21r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51B21
5-PRIME, mRNA sequence
Length = 564
Score = 145 bits (73), Expect = 3e-033
Identities = 193/233 (82%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 328 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 387
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | ||| || |||||||| ||||| |||||||| |||
Sbjct: 388 ggcttcaagctgggtgaccatgtggctgtggggacatatgtcaactcatgccgtgactgt 447
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
|| |||||||||| || || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 448 gacaactgcaatagcttcctcgagaaccactgctcaaaatttgttttcactttcaatggt 507
Query: 723 attgatacagatggaactgtcacaaaggggggttactccactcacattgtagt 775
||||||| ||||| |||||||||||||| || || |||| |||| |||||||
Sbjct: 508 gttgatacggatggtactgtcacaaagggaggatattccagtcacgttgtagt 560
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 160 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 219
Query: 435 aaccg 439
|||||
Sbjct: 220 aaccg 224
>gb|AL506332.1|AL506332 AL506332 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02M11T 5',
mRNA sequence
Length = 700
Score = 119 bits (60), Expect = 2e-025
Identities = 174/211 (82%), Gaps = 1/211 (0%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 430 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 489
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | ||| || |||||||| ||||| |||||||| ||
Sbjct: 490 ggcttcaagctgggtgaccatgtggctgtggggacatatgtcaactcatgccgtgactgn 549
Query: 663 gagaactgcaatatctctctagacaaccattgcccaaaagcagtttatactttcaatggc 722
|| |||||||||| || || || ||||| ||| ||||| |||| |||||||||||
Sbjct: 550 gacaactgcaatagcttcctcgagaaccactgctcaaaatttgttttcactttcaatggt 609
Query: 723 attgatacagat-ggaactgtcacaaagggg 752
||||||| ||| || |||||||||||||||
Sbjct: 610 gttgatacggatgggtactgtcacaaagggg 640
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 262 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 321
Query: 435 aaccg 439
|||||
Sbjct: 322 aaccg 326
>gb|AV931399.1|AV931399 AV931399 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19k10 3', mRNA sequence
Length = 683
Score = 113 bits (57), Expect = 1e-023
Identities = 126/149 (84%)
Strand = Plus / Minus
Query: 840 tgtgctggaataaccgtgtatactccaatgatgcgacacaacatgaaccaacctggaaag 899
||||||||||| || ||||| ||||| |||| |||||| ||||||||||| || || |||
Sbjct: 652 tgtgctggaatcactgtgtacactccgatgacgcgacataacatgaaccagccagggaag 593
Query: 900 tcacttggggtcattggtcttggtggtctgggtcacatggctgtgaaatttggtaaagca 959
|||||||| || | ||||||||||| ||||||||||||| ||||||||||| |||||
Sbjct: 592 tcacttggtgttgtcggtcttggtgggttgggtcacatggcagtgaaatttgggaaagcc 533
Query: 960 tttggtcttaaggtcacagttttcagtac 988
||||| || ||||| || ||||| |||||
Sbjct: 532 tttggactgaaggtgactgttttgagtac 504
>gb|BI956152.1|BI956152 HVSMEm0025N07f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025N07f, mRNA sequence
Length = 685
Score = 105 bits (53), Expect = 3e-021
Identities = 113/133 (84%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttgtaactcaggttggtgcagatgtcaaa 602
||||| || |||||||||||||| |||||||||||||| |||||||| |||| |||||
Sbjct: 427 cctttagtccctgggcatgagattgctggagttgtaaccgaggttggttcagacgtcaag 486
Query: 603 ggctttaaagtgggtgaccatgtaggtgttggaacatatgtgaactcttgccgtgattgt 662
||||| || ||||||||||||| | || || |||||||| ||||| |||||||| |||
Sbjct: 487 ggcttcaagctgggtgaccatgtggctggggggacatatgtcaactcatgccgtgactgt 546
Query: 663 gagaactgcaata 675
|| ||||||||||
Sbjct: 547 gacaactgcaata 559
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 259 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 318
Query: 435 aaccg 439
|||||
Sbjct: 319 aaccg 323
>gb|BJ456704.1|BJ456704 BJ456704 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak23a07 3', mRNA sequence
Length = 674
Score = 85.7 bits (43), Expect = 3e-015
Identities = 102/122 (83%)
Strand = Plus / Minus
Query: 867 atgatgcgacacaacatgaaccaacctggaaagtcacttggggtcattggtcttggtggt 926
|||| |||||| ||||||||||| || || ||||||||||| || | |||||||||||
Sbjct: 659 atgacgcgacataacatgaaccagccagggaagtcacttggtgttgtcggtcttggtggg 600
Query: 927 ctgggtcacatggctgtgaaatttggtaaagcatttggtcttaaggtcacagttttcagt 986
||||||||||| | ||||||||||| ||||| ||||| || ||||| || ||||| |||
Sbjct: 599 ttgggtcacatgncagtgaaatttgggaaagcctttggactgaaggtgactgttttgagt 540
Query: 987 ac 988
||
Sbjct: 539 ac 538
>gb|BQ467644.1|BQ467644 HS04G20r HS Hordeum vulgare subsp. vulgare cDNA clone HS04G20
5-PRIME, mRNA sequence
Length = 411
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 212 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 271
Query: 435 aaccg 439
|||||
Sbjct: 272 aaccg 276
Score = 40.1 bits (20), Expect = 0.14
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagt 574
||||| || |||||||||||||| ||||||||
Sbjct: 380 cctttagtccctgggcatgagattgctggagt 411
>gb|CA004275.1|CA004275 HS17B20r HS Hordeum vulgare subsp. vulgare cDNA clone HS17B20
5-PRIME, mRNA sequence
Length = 413
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 212 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 271
Query: 435 aaccg 439
|||||
Sbjct: 272 aaccg 276
Score = 44.1 bits (22), Expect = 0.009
Identities = 31/34 (91%)
Strand = Plus / Plus
Query: 543 cctttggttcctgggcatgagatagctggagttg 576
||||| || |||||||||||||| ||||||||||
Sbjct: 380 cctttagtccctgggcatgagattgctggagttg 413
>gb|CA012143.1|CA012143 HT04J04r HT Hordeum vulgare subsp. vulgare cDNA clone HT04J04
5-PRIME, mRNA sequence
Length = 442
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 375 ggcaactgcaaagcttgggcagcgagagatccttctggagttctctcaccacacagtttt 434
|||||||||| ||||||||||| | ||||||||||||| | ||||| || |||| |||
Sbjct: 257 ggcaactgcagtgcttgggcagcaaaagatccttctggactactctccccgcacactttc 316
Query: 435 aaccg 439
|||||
Sbjct: 317 aaccg 321
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 72,922
Number of Sequences: 312970
Number of extensions: 72922
Number of successful extensions: 18321
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18278
Number of HSP's gapped (non-prelim): 37
length of query: 991
length of database: 175,134,539
effective HSP length: 19
effective length of query: 972
effective length of database: 169,188,109
effective search space: 164450841948
effective search space used: 164450841948
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)