BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2485885.2.1
(1063 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. v... 182 2e-044
gb|AL450622.1|AL450622 AL450622 Hordeum vulgare Barke etiol... 78 7e-013
gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. v... 62 4e-008
gb|BI947771.1|BI947771 HVSMEl0006N11f Hordeum vulgare spike... 56 2e-006
gb|BF262509.1|BF262509 HV_CEa0004C17f Hordeum vulgare seedl... 54 1e-005
gb|BG417500.2|BG417500 HVSMEk0017N08f Hordeum vulgare testa... 54 1e-005
gb|CA001112.1|CA001112 HS05G15u HS Hordeum vulgare subsp. v... 48 6e-004
gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. v... 48 6e-004
gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. v... 48 6e-004
emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for p... 48 6e-004
gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedl... 42 0.037
gb|BF256954.2|BF256954 HVSMEf0011H12f Hordeum vulgare seedl... 42 0.037
gb|BI957863.1|BI957863 HVSMEn0011M19f Hordeum vulgare rachi... 42 0.037
gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subs... 42 0.037
gb|BM442817.2|BM442817 EBro02_SQ001_G03_R root, 3 week, hyd... 42 0.037
gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. v... 42 0.037
gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedl... 40 0.15
gb|BF065670.2|BF065670 HV_CEb0013E24f Hordeum vulgare seedl... 40 0.15
gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedl... 40 0.15
gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. v... 40 0.15
gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. v... 40 0.15
gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hyd... 40 0.15
gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. v... 40 0.15
gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. v... 40 0.15
>gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47C08
5-PRIME, mRNA sequence
Length = 355
Score = 182 bits (92), Expect = 2e-044
Identities = 262/316 (82%), Gaps = 2/316 (0%)
Strand = Plus / Plus
Query: 279 gccctcggacgggaacctcgcggagctgcttccggcggatttcttggcgaggacgaagga 338
||||||||||| | |||||| ||||||||| |||| || || |||| |||||| |||||
Sbjct: 19 gccctcggacgcggacctcggggagctgctcccggaggggtttttggagaggacaaagga 78
Query: 339 tagagggc-tcgtgtggcctaccaaggcgccgcagaaggagatactcgcccacgccgccg 397
| ||||| | |||||||| |||||||| |||||||||||||| || || |||||| | |
Sbjct: 79 ga-agggcatggtgtggccgaccaaggctccgcagaaggagatcctggcgcacgcctctg 137
Query: 398 tgggaggcttcgtcacgcactgtggctggaactcggtcctcgagagcctctggcatggcg 457
|||| |||||||| |||||| | || ||||||||| ||| |||||||| ||| ||||
Sbjct: 138 tggggggcttcgtgacgcacggcgggtggaactcgaccctggagagcctgtggttcggcg 197
Query: 458 tgccgatggtgccatggccgcttggcgccgagcagcactacaacgcgttcacgctggtgg 517
|||||||||||||||||||||| ||| ||||||||| |||||||||||||||||||
Sbjct: 198 tgccgatggtgccatggccgctgtacgcggagcagcacctgaacgcgttcacgctggtgg 257
Query: 518 ccgacatgggcgtcgccgtggcgctgaacgtggaaaggaagaggaagaatttcgtggagg 577
|| |||||||||| |||||||| || | ||||| ||||||||||| || ||||| | |
Sbjct: 258 cctacatgggcgtggccgtggccatggaggtggacaggaagaggaacaacttcgtcgccg 317
Query: 578 cgaccgagctggagcg 593
|| | |||||||||||
Sbjct: 318 cgtcggagctggagcg 333
>gb|AL450622.1|AL450622 AL450622 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03H20u 5', mRNA sequence
Length = 475
Score = 77.8 bits (39), Expect = 7e-013
Identities = 138/171 (80%)
Strand = Plus / Plus
Query: 429 ctcggtcctcgagagcctctggcatggcgtgccgatggtgccatggccgcttggcgccga 488
|||||| || |||||||| ||||| ||| | ||||||| ||| ||||| || ||| ||
Sbjct: 1 ctcggtgctggagagcctgtggcacggcataccgatggcgccgtggccactctacgcgga 60
Query: 489 gcagcactacaacgcgttcacgctggtggccgacatgggcgtcgccgtggcgctgaacgt 548
||||||| |||||||||| ||| || ||||||||||||||||||||| |||| || ||
Sbjct: 61 gcagcacctcaacgcgttcgagctcgtcgccgacatgggcgtcgccgtgccgctcaaggt 120
Query: 549 ggaaaggaagaggaagaatttcgtggaggcgaccgagctggagcgagcggt 599
||| ||| | || || |||||||||||| | ||||||||||| |||||
Sbjct: 121 ggaccggaggcggggcaacttcgtggaggcggcggagctggagcgggcggt 171
>gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. vulgare cDNA clone HI01C06
3-PRIME, mRNA sequence
Length = 668
Score = 61.9 bits (31), Expect = 4e-008
Identities = 55/63 (87%)
Strand = Plus / Minus
Query: 382 ctcgcccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgag 441
|||||||||||||| |||| || ||| |||||||||| |||||||||||||| || |||
Sbjct: 591 ctcgcccacgccgcgctgggcggattcctcacgcactgcggctggaactcggtgctggag 532
Query: 442 agc 444
|||
Sbjct: 531 agc 529
>gb|BI947771.1|BI947771 HVSMEl0006N11f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0006N11f, mRNA sequence
Length = 853
Score = 56.0 bits (28), Expect = 2e-006
Identities = 67/80 (83%)
Strand = Plus / Plus
Query: 414 gcactgtggctggaactcggtcctcgagagcctctggcatggcgtgccgatggtgccatg 473
|||||| || ||||||||||| |||||||||||| ||| |||||||| ||||| | ||
Sbjct: 11 gcactgcgggtggaactcggtgctcgagagcctcgcgcacggcgtgcccatggtcgcctg 70
Query: 474 gccgcttggcgccgagcagc 493
|||||| |||||||||||
Sbjct: 71 gccgctcttcgccgagcagc 90
>gb|BF262509.1|BF262509 HV_CEa0004C17f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0004C17f, mRNA sequence
Length = 873
Score = 54.0 bits (27), Expect = 1e-005
Identities = 51/59 (86%)
Strand = Plus / Plus
Query: 386 cccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgagagc 444
|||||||||||||||| |||||| | |||||||| || |||||||| || || ||||||
Sbjct: 36 cccacgccgccgtgggtggcttcctgacgcactgcgggtggaactctgtgctggagagc 94
>gb|BG417500.2|BG417500 HVSMEk0017N08f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0017N08f, mRNA sequence
Length = 824
Score = 54.0 bits (27), Expect = 1e-005
Identities = 54/63 (85%)
Strand = Plus / Plus
Query: 382 ctcgcccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgag 441
|||||||||||||| |||| || ||| | |||||||| |||||||||||||| || |||
Sbjct: 96 ctcgcccacgccgcgctgggcggattcctgacgcactgcggctggaactcggtgctggag 155
Query: 442 agc 444
|||
Sbjct: 156 agc 158
>gb|CA001112.1|CA001112 HS05G15u HS Hordeum vulgare subsp. vulgare cDNA clone HS05G15
3-PRIME, mRNA sequence
Length = 560
Score = 48.1 bits (24), Expect = 6e-004
Identities = 42/48 (87%)
Strand = Plus / Minus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggt 434
|||||||||| | || || ||||| |||||||| ||||||||||||||
Sbjct: 529 ccacgccgccctcggcgggttcgtgacgcactgcggctggaactcggt 482
>gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. vulgare cDNA clone HT14O18
5-PRIME, mRNA sequence
Length = 622
Score = 48.1 bits (24), Expect = 6e-004
Identities = 42/48 (87%)
Strand = Plus / Plus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggt 434
|||||||||| | || || ||||| |||||||| ||||||||||||||
Sbjct: 322 ccacgccgccctcggcgggttcgtgacgcactgcggctggaactcggt 369
>gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61B05
5-PRIME, mRNA sequence
Length = 565
Score = 48.1 bits (24), Expect = 6e-004
Identities = 36/40 (90%)
Strand = Plus / Plus
Query: 395 ccgtgggaggcttcgtcacgcactgtggctggaactcggt 434
||||||| || |||||||||||||| || |||||||||||
Sbjct: 493 ccgtggggggattcgtcacgcactgcggttggaactcggt 532
>emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for putative glycosyltransferase (sf2
gene)
Length = 863
Score = 48.1 bits (24), Expect = 6e-004
Identities = 42/48 (87%)
Strand = Plus / Plus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggt 434
|||||||||| | || || ||||| |||||||| ||||||||||||||
Sbjct: 588 ccacgccgccctcggcgggttcgtgacgcactgcggctggaactcggt 635
>gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017B13f, mRNA sequence
Length = 875
Score = 42.1 bits (21), Expect = 0.037
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 144 cgtggtgttcctatgcttcggcagc 168
|||||||||||| ||||||||||||
Sbjct: 23 cgtggtgttcctctgcttcggcagc 47
>gb|BF256954.2|BF256954 HVSMEf0011H12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011H12f, mRNA sequence
Length = 540
Score = 42.1 bits (21), Expect = 0.037
Identities = 36/41 (87%)
Strand = Plus / Plus
Query: 111 cgtgcgctggctcgactcgcagcctccgagctccgtggtgt 151
|||||||||||| ||| ||||||||| ||||||||||||
Sbjct: 339 cgtgcgctggctggacgagcagcctcccggctccgtggtgt 379
>gb|BI957863.1|BI957863 HVSMEn0011M19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0011M19f, mRNA sequence
Length = 1178
Score = 42.1 bits (21), Expect = 0.037
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 412 acgcactgtggctggaactcggtcctcgagagc 444
|||||||| |||||||||||||| || ||||||
Sbjct: 540 acgcactgcggctggaactcggtgctggagagc 508
>gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200036E05F1, mRNA sequence
Length = 360
Score = 42.1 bits (21), Expect = 0.037
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 319 ttcttggcgaggacgaaggatagagggctcgtg 351
||||||| |||||| ||||| ||||||||||||
Sbjct: 64 ttcttggagaggaccaaggacagagggctcgtg 96
>gb|BM442817.2|BM442817 EBro02_SQ001_G03_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ001_G03 5', mRNA sequence
Length = 419
Score = 42.1 bits (21), Expect = 0.037
Identities = 33/37 (89%)
Strand = Plus / Plus
Query: 410 tcacgcactgtggctggaactcggtcctcgagagcct 446
|||||||| | ||||||||||||| |||||||||||
Sbjct: 2 tcacgcacagcggctggaactcggcgctcgagagcct 38
>gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. vulgare cDNA clone HI10O06
5-PRIME, mRNA sequence
Length = 635
Score = 42.1 bits (21), Expect = 0.037
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 319 ttcttggcgaggacgaaggatagagggctcgtg 351
||||||| |||||| ||||| ||||||||||||
Sbjct: 465 ttcttggagaggaccaaggacagagggctcgtg 497
>gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0008E02f, mRNA sequence
Length = 718
Score = 40.1 bits (20), Expect = 0.15
Identities = 50/60 (83%)
Strand = Plus / Plus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgagagcct 446
|||| ||||||| || | |||| |||||||| | || |||||||||| |||||||||||
Sbjct: 502 ccaccccgccgtcggcgccttcctcacgcacagcggttggaactcggcgctcgagagcct 561
>gb|BF065670.2|BF065670 HV_CEb0013E24f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0013E24f, mRNA sequence
Length = 573
Score = 40.1 bits (20), Expect = 0.15
Identities = 50/60 (83%)
Strand = Plus / Plus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgagagcct 446
|||| ||||||| || | |||| |||||||| | || |||||||||| |||||||||||
Sbjct: 41 ccaccccgccgtcggggccttcctcacgcacagcggttggaactcggcgctcgagagcct 100
>gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006H07f, mRNA sequence
Length = 505
Score = 40.1 bits (20), Expect = 0.15
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 404 gcttcgtcacgcactgtggctggaactc 431
|||||||||| ||||| |||||||||||
Sbjct: 99 gcttcgtcacccactgcggctggaactc 126
>gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
5-PRIME, mRNA sequence
Length = 571
Score = 40.1 bits (20), Expect = 0.15
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 404 gcttcgtcacgcactgtggctggaactc 431
|||||||||| ||||| |||||||||||
Sbjct: 179 gcttcgtcacccactgcggctggaactc 206
>gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
3-PRIME, mRNA sequence
Length = 570
Score = 40.1 bits (20), Expect = 0.15
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 404 gcttcgtcacgcactgtggctggaactc 431
|||||||||| ||||| |||||||||||
Sbjct: 392 gcttcgtcacccactgcggctggaactc 365
>gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B12 5', mRNA sequence
Length = 552
Score = 40.1 bits (20), Expect = 0.15
Identities = 32/36 (88%)
Strand = Plus / Plus
Query: 406 ttcgtcacgcactgtggctggaactcggtcctcgag 441
||||| |||||||| || ||||||||||| ||||||
Sbjct: 192 ttcgtgacgcactgcggatggaactcggtgctcgag 227
>gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. vulgare cDNA clone HT14F01
5-PRIME, mRNA sequence
Length = 510
Score = 40.1 bits (20), Expect = 0.15
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 404 gcttcgtcacgcactgtggctggaactc 431
|||||||||| ||||| |||||||||||
Sbjct: 218 gcttcgtcacccactgcggctggaactc 245
>gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. vulgare cDNA clone HV08I24
5-PRIME, mRNA sequence
Length = 647
Score = 40.1 bits (20), Expect = 0.15
Identities = 50/60 (83%)
Strand = Plus / Plus
Query: 387 ccacgccgccgtgggaggcttcgtcacgcactgtggctggaactcggtcctcgagagcct 446
|||| ||||||| || | |||| |||||||| | || |||||||||| |||||||||||
Sbjct: 152 ccaccccgccgtcggcgccttcctcacgcacagcggttggaactcggcgctcgagagcct 211
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 121,652
Number of Sequences: 312970
Number of extensions: 121652
Number of successful extensions: 34700
Number of sequences better than 0.5: 24
Number of HSP's better than 0.5 without gapping: 24
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 34661
Number of HSP's gapped (non-prelim): 38
length of query: 1063
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1044
effective length of database: 169,188,109
effective search space: 176632385796
effective search space used: 176632385796
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)