BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478343.2.1
(694 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedl... 68 4e-010
gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. v... 68 4e-010
gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. v... 68 4e-010
gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. v... 68 4e-010
gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. v... 62 3e-008
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 56 2e-006
gb|CB879235.1|CB879235 HP11G23T HP Hordeum vulgare subsp. v... 56 2e-006
gb|BF628779.2|BF628779 HVSMEb0008B22f Hordeum vulgare seedl... 50 1e-004
gb|BF628789.2|BF628789 HVSMEb0008D18f Hordeum vulgare seedl... 50 1e-004
gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. v... 50 1e-004
gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green... 48 4e-004
gb|BM444331.1|BM444331 EBem09_SQ004_N12_R embryo, 1 Day ger... 48 4e-004
gb|BG417500.2|BG417500 HVSMEk0017N08f Hordeum vulgare testa... 42 0.024
gb|BU994136.1|BU994136 HM05N21r HM Hordeum vulgare subsp. v... 42 0.024
gb|CB880799.1|CB880799 HM07N21w HM Hordeum vulgare subsp. v... 42 0.024
gb|BF267582.3|BF267582 HV_CEa0018F10f Hordeum vulgare seedl... 40 0.094
gb|BF620291.2|BF620291 HVSMEc0019F18f Hordeum vulgare seedl... 38 0.37
gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedl... 38 0.37
gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedl... 38 0.37
gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subs... 38 0.37
>gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006H07f, mRNA sequence
Length = 505
Score = 67.9 bits (34), Expect = 4e-010
Identities = 55/62 (88%)
Strand = Plus / Minus
Query: 622 cacggcctccacggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtg 681
||||||||||| ||||||||||||||||||||||||| ||||| || |||||||| ||
Sbjct: 142 cacggcctccagcgtggagttccagccgcagtgggtgacgaagcacccgacggccggatg 83
Query: 682 cg 683
||
Sbjct: 82 cg 81
>gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
5-PRIME, mRNA sequence
Length = 571
Score = 67.9 bits (34), Expect = 4e-010
Identities = 55/62 (88%)
Strand = Plus / Minus
Query: 622 cacggcctccacggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtg 681
||||||||||| ||||||||||||||||||||||||| ||||| || |||||||| ||
Sbjct: 222 cacggcctccagcgtggagttccagccgcagtgggtgacgaagcacccgacggccggatg 163
Query: 682 cg 683
||
Sbjct: 162 cg 161
>gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
3-PRIME, mRNA sequence
Length = 570
Score = 67.9 bits (34), Expect = 4e-010
Identities = 55/62 (88%)
Strand = Plus / Plus
Query: 622 cacggcctccacggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtg 681
||||||||||| ||||||||||||||||||||||||| ||||| || |||||||| ||
Sbjct: 349 cacggcctccagcgtggagttccagccgcagtgggtgacgaagcacccgacggccggatg 408
Query: 682 cg 683
||
Sbjct: 409 cg 410
>gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. vulgare cDNA clone HT14F01
5-PRIME, mRNA sequence
Length = 510
Score = 67.9 bits (34), Expect = 4e-010
Identities = 55/62 (88%)
Strand = Plus / Minus
Query: 622 cacggcctccacggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtg 681
||||||||||| ||||||||||||||||||||||||| ||||| || |||||||| ||
Sbjct: 261 cacggcctccagcgtggagttccagccgcagtgggtgacgaagcacccgacggccggatg 202
Query: 682 cg 683
||
Sbjct: 201 cg 200
>gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. vulgare cDNA clone HR01O04
3-PRIME, mRNA sequence
Length = 532
Score = 61.9 bits (31), Expect = 3e-008
Identities = 73/87 (83%)
Strand = Plus / Plus
Query: 608 ggcacgccctccgacacggcctccacggtggagttccagccgcagtgggtgaagaagccg 667
||||||||| || || |||||||| ||||||||||| ||||||||||||| ||| |
Sbjct: 413 ggcacgcccagcgccatggcctccagcgtggagttccacccgcagtgggtgacgaatcac 472
Query: 668 cccacggccgggtgcgcgagcacctcc 694
||||||| |||||||| ||| ||||||
Sbjct: 473 cccacggacgggtgcgagaggacctcc 499
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 640 gttccagccgcagtgggtgaagaagccgcccacggccgggtgcg 683
||||||||||||||| || | |||||||||||| ||||||||||
Sbjct: 323 gttccagccgcagtgcgtcaggaagccgcccaccgccgggtgcg 280
>gb|CB879235.1|CB879235 HP11G23T HP Hordeum vulgare subsp. vulgare cDNA clone HP11G23
5-PRIME, mRNA sequence
Length = 622
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 640 gttccagccgcagtgggtgaagaagccgcccacggccgggtgcg 683
||||||||||||||| || | |||||||||||| ||||||||||
Sbjct: 71 gttccagccgcagtgcgtcaggaagccgcccaccgccgggtgcg 28
>gb|BF628779.2|BF628779 HVSMEb0008B22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0008B22f, mRNA sequence
Length = 741
Score = 50.1 bits (25), Expect = 1e-004
Identities = 43/49 (87%)
Strand = Plus / Minus
Query: 634 ggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtgc 682
||||||||||||||||||||| |||| |||| |||| | |||||||||
Sbjct: 488 ggtggagttccagccgcagtgtgtgaggaagacgccgagcgccgggtgc 440
>gb|BF628789.2|BF628789 HVSMEb0008D18f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0008D18f, mRNA sequence
Length = 769
Score = 50.1 bits (25), Expect = 1e-004
Identities = 43/49 (87%)
Strand = Plus / Minus
Query: 634 ggtggagttccagccgcagtgggtgaagaagccgcccacggccgggtgc 682
||||||||||||||||||||| |||| |||| |||| | |||||||||
Sbjct: 488 ggtggagttccagccgcagtgtgtgaggaagacgccgagcgccgggtgc 440
>gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. vulgare cDNA clone HI01C06
3-PRIME, mRNA sequence
Length = 668
Score = 50.1 bits (25), Expect = 1e-004
Identities = 49/57 (85%)
Strand = Plus / Plus
Query: 638 gagttccagccgcagtgggtgaagaagccgcccacggccgggtgcgcgagcacctcc 694
||||||||||||||||| |||| ||| ||||||| || | ||| ||||||||||||
Sbjct: 542 gagttccagccgcagtgcgtgaggaatccgcccagcgcggcgtgggcgagcacctcc 598
>gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012J18f, mRNA sequence
Length = 855
Score = 48.1 bits (24), Expect = 4e-004
Identities = 27/28 (96%)
Strand = Plus / Minus
Query: 628 ctccacggtggagttccagccgcagtgg 655
||||||||| ||||||||||||||||||
Sbjct: 321 ctccacggtcgagttccagccgcagtgg 294
>gb|BM444331.1|BM444331 EBem09_SQ004_N12_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_N12 5', mRNA sequence
Length = 340
Score = 48.1 bits (24), Expect = 4e-004
Identities = 45/52 (86%)
Strand = Plus / Minus
Query: 626 gcctccacggtggagttccagccgcagtgggtgaagaagccgcccacggccg 677
||||||| ||||||||||| |||||||| || | ||||| |||||||||||
Sbjct: 166 gcctccagcgtggagttccacccgcagtgcgtcacgaagcagcccacggccg 115
>gb|BG417500.2|BG417500 HVSMEk0017N08f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0017N08f, mRNA sequence
Length = 824
Score = 42.1 bits (21), Expect = 0.024
Identities = 48/57 (84%)
Strand = Plus / Minus
Query: 638 gagttccagccgcagtgggtgaagaagccgcccacggccgggtgcgcgagcacctcc 694
||||||||||||||||| || | ||| ||||||| || | ||| ||||||||||||
Sbjct: 145 gagttccagccgcagtgcgtcaggaatccgcccagcgcggcgtgggcgagcacctcc 89
>gb|BU994136.1|BU994136 HM05N21r HM Hordeum vulgare subsp. vulgare cDNA clone HM05N21
5-PRIME, mRNA sequence
Length = 554
Score = 42.1 bits (21), Expect = 0.024
Identities = 33/37 (89%)
Strand = Plus / Minus
Query: 647 ccgcagtgggtgaagaagccgcccacggccgggtgcg 683
|||||||| || | |||||| ||||||||||||||||
Sbjct: 144 ccgcagtgcgtcatgaagccccccacggccgggtgcg 108
>gb|CB880799.1|CB880799 HM07N21w HM Hordeum vulgare subsp. vulgare cDNA clone HM07N21
3-PRIME, mRNA sequence
Length = 550
Score = 42.1 bits (21), Expect = 0.024
Identities = 33/37 (89%)
Strand = Plus / Plus
Query: 647 ccgcagtgggtgaagaagccgcccacggccgggtgcg 683
|||||||| || | |||||| ||||||||||||||||
Sbjct: 411 ccgcagtgcgtcatgaagccccccacggccgggtgcg 447
>gb|BF267582.3|BF267582 HV_CEa0018F10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0018F10f, mRNA sequence
Length = 602
Score = 40.1 bits (20), Expect = 0.094
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 635 gtggagttccagccgcagtgggtgaagaagccgccc 670
||||||||||| |||||||| || | ||||||||||
Sbjct: 53 gtggagttccacccgcagtgcgtcaggaagccgccc 18
>gb|BF620291.2|BF620291 HVSMEc0019F18f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0019F18f, mRNA sequence
Length = 689
Score = 38.2 bits (19), Expect = 0.37
Identities = 31/35 (88%)
Strand = Plus / Minus
Query: 660 agaagccgcccacggccgggtgcgcgagcacctcc 694
|||| ||| ||||||||||||||| |||||||||
Sbjct: 644 agaacccggccacggccgggtgcggaagcacctcc 610
>gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0009B02f, mRNA sequence
Length = 782
Score = 38.2 bits (19), Expect = 0.37
Identities = 31/35 (88%)
Strand = Plus / Minus
Query: 638 gagttccagccgcagtgggtgaagaagccgcccac 672
|||||||| ||||||||||| | ||| ||||||||
Sbjct: 111 gagttccacccgcagtgggtcacgaacccgcccac 77
>gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012H19f, mRNA sequence
Length = 847
Score = 38.2 bits (19), Expect = 0.37
Identities = 31/35 (88%)
Strand = Plus / Minus
Query: 660 agaagccgcccacggccgggtgcgcgagcacctcc 694
|||| ||| |||||||||||||| ||||||||||
Sbjct: 444 agaacccggccacggccgggtgccggagcacctcc 410
>gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058E12F1, mRNA sequence
Length = 360
Score = 38.2 bits (19), Expect = 0.37
Identities = 31/35 (88%)
Strand = Plus / Minus
Query: 660 agaagccgcccacggccgggtgcgcgagcacctcc 694
|||| ||| |||||||||||||| ||||||||||
Sbjct: 77 agaacccggccacggccgggtgccggagcacctcc 43
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 72,034
Number of Sequences: 312970
Number of extensions: 72034
Number of successful extensions: 21880
Number of sequences better than 0.5: 21
Number of HSP's better than 0.5 without gapping: 20
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 21848
Number of HSP's gapped (non-prelim): 33
length of query: 694
length of database: 175,134,539
effective HSP length: 19
effective length of query: 675
effective length of database: 169,188,109
effective search space: 114201973575
effective search space used: 114201973575
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)