BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448637.2.1
(1190 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 113 1e-023
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 70 2e-010
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 70 2e-010
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 70 2e-010
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 70 2e-010
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 70 2e-010
gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiol... 58 7e-007
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 58 7e-007
gb|CA006891.1|CA006891 HU06A02r HU Hordeum vulgare subsp. v... 52 4e-005
gb|BI949395.1|BI949395 HVSMEl0013K23f Hordeum vulgare spike... 48 7e-004
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 44 0.010
gb|BE411008.1|BE411008 ISC001D05F990323 ITEC ISC Barley Lea... 42 0.041
gb|BE411741.1|BE411741 ISC008.G08F990804 ITEC ISC Barley Le... 42 0.041
gb|BE411855.1|BE411855 ISC010.A02F990902 ITEC ISC Barley Le... 42 0.041
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 42 0.041
gb|AV916160.1|AV916160 AV916160 K. Sato unpublished cDNA li... 42 0.041
gb|AV921285.1|AV921285 AV921285 K. Sato unpublished cDNA li... 42 0.041
gb|CB882086.1|CB882086 HM11N04w HM Hordeum vulgare subsp. v... 42 0.041
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak38k12 5', mRNA sequence
Length = 633
Score = 113 bits (57), Expect = 1e-023
Identities = 162/197 (82%)
Strand = Plus / Minus
Query: 811 agcaagtgtggcagcttcagggaagccagaaagcatcctatgcccagcatttagaccaga 870
||||||||| |||||||||||||||||||||| ||| | || ||||||| | ||||||
Sbjct: 513 agcaagtgttgcagcttcagggaagccagaaattatcttctgttcagcattcaaaccaga 454
Query: 871 ttgggagggagatatagccatcgacactctttgcacagcagaaatgacactacgaacata 930
| | |||||||| ||||||||||||||||| || || ||||| | | || || |||||
Sbjct: 453 tcgagagggagagatagccatcgacactctctgaacggcagagacaatgctgcggacata 394
Query: 931 ttgacgggccattgctgcaacgctgtcctgaaggtgcatctcatacgggaattgaaaggc 990
|| |||||||| | |||||| || || || || |||||||| || ||||||||||||||
Sbjct: 393 ctgccgggccatggttgcaacactatcttggagatgcatctcgtaagggaattgaaaggc 334
Query: 991 gattgtcagcacagatc 1007
||||||||||| ||||
Sbjct: 333 aattgtcagcactgatc 317
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Minus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || |||||||| | ||| | ||||| ||
Sbjct: 242 tcacacgaaagaccagttgacgaagcagaaggcaaggcagtgcacattgttgtcctcacc 183
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 182 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 123
Query: 505 cac 507
|||
Sbjct: 122 cac 120
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Minus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || |||||||| | ||| | ||||| ||
Sbjct: 219 tcacacgaaagaccagttgacgaagcagaaggcaaggcagtgcacattgttgtcctcacc 160
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 159 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 100
Query: 505 cac 507
|||
Sbjct: 99 cac 97
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || |||||||| | ||| | ||||| ||
Sbjct: 203 tcacacgaaagaccagttgacgaagcagaaggcaaggcagtgcacattgttgtcctcacc 262
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 263 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 322
Query: 505 cac 507
|||
Sbjct: 323 cac 325
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || |||||||| | ||| | ||||| ||
Sbjct: 206 tcacacgaaagaccagttgacgaagcagaaggcaaggcagtgcacattgttgtcctcacc 265
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 266 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 325
Query: 505 cac 507
|||
Sbjct: 326 cac 328
Score = 61.9 bits (31), Expect = 4e-008
Identities = 121/151 (80%)
Strand = Plus / Plus
Query: 605 aggtcttgcagcgcaacaagagatgtttctaacatgtccagccctgccttgtttgcaaac 664
|||||||| || ||||| | ||||||||| ||||||| | ||| || ||||||||||
Sbjct: 426 aggtcttgaagggcaacgaaagatgtttccaacatgttaatccccatctcgtttgcaaac 485
Query: 665 gtaaacataggtttctccttaaaggagcagcacagaacagcatctggatgatgccagagc 724
||||| | ||| || ||||| || || ||||||| || ||||||| ||||| |||||||
Sbjct: 486 gtaaatacaggcttttccttgaaagaacagcacaaaatagcatcttcatgatcccagagc 545
Query: 725 attttcaacaatgcttcaccagctccatctg 755
||| |||| || | ||| |||||| ||||||
Sbjct: 546 attctcaataaagattccccagcttcatctg 576
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || |||||||| | ||| | ||||| ||
Sbjct: 198 tcacacgaaagaccagttgacgaagcagaaggcaaggcagtgcacattgttgtcctcacc 257
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 258 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 317
Query: 505 cac 507
|||
Sbjct: 318 cac 320
Score = 61.9 bits (31), Expect = 4e-008
Identities = 121/151 (80%)
Strand = Plus / Plus
Query: 605 aggtcttgcagcgcaacaagagatgtttctaacatgtccagccctgccttgtttgcaaac 664
|||||||| || ||||| | ||||||||| ||||||| | ||| || ||||||||||
Sbjct: 418 aggtcttgaagggcaacgaaagatgtttccaacatgttaatccccatctcgtttgcaaac 477
Query: 665 gtaaacataggtttctccttaaaggagcagcacagaacagcatctggatgatgccagagc 724
||||| | ||| || ||||| || || ||||||| || ||||||| ||||| |||||||
Sbjct: 478 gtaaatacaggcttttccttgaaagaacagcacaaaatagcatcttcatgatcccagagc 537
Query: 725 attttcaacaatgcttcaccagctccatctg 755
||| |||| || | ||| |||||| ||||||
Sbjct: 538 attctcaataaagattccccagcttcatctg 568
>gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04F09r 3', mRNA sequence
Length = 573
Score = 58.0 bits (29), Expect = 7e-007
Identities = 88/108 (81%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
||||||||| |||||||||||||||||||| || ||||| || | ||| | ||||| ||
Sbjct: 254 tcacacgaaagaccagttgacgaagcagaaggcaaggcantgcacattgttgtcctcacc 313
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccat 492
|| || ||||| |||| ||| | ||||| || ||||||||||||||
Sbjct: 314 gactactttccatgctatagcattctcgaaggagacatggcggcccat 361
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 58.0 bits (29), Expect = 7e-007
Identities = 83/101 (82%)
Strand = Plus / Minus
Query: 655 gtttgcaaacgtaaacataggtttctccttaaaggagcagcacagaacagcatctggatg 714
||||||||||||||| | ||| || ||||| || || ||||||| || ||||||| |||
Sbjct: 153 gtttgcaaacgtaaatacaggcttttccttgaaagaacagcacaaaatagcatcttcatg 94
Query: 715 atgccagagcattttcaacaatgcttcaccagctccatctg 755
|| |||||||||| |||| || | ||| |||||| ||||||
Sbjct: 93 atcccagagcattctcaataaagattccccagcttcatctg 53
Score = 54.0 bits (27), Expect = 1e-005
Identities = 99/123 (80%)
Strand = Plus / Minus
Query: 385 tcacacgaaggaccagttgacgaagcagaacgccaggcagtggatgttgctatcctcgcc 444
|||| |||| ||||||||||| |||||||| || |||||||| | ||| | ||||| ||
Sbjct: 423 tcactcgaaagaccagttgactaagcagaaggcaaggcagtgcacattgttgtcctcacc 364
Query: 445 gagcaccttccacgctacagcttggtcgaaagaaacatggcggcccattcctgacatgca 504
|| || ||||| |||| ||| | ||||| || |||||||||||||| || |||| |||
Sbjct: 363 gactactttccatgctatagcattctcgaaggagacatggcggcccatcccagacaagca 304
Query: 505 cac 507
|||
Sbjct: 303 cac 301
>gb|CA006891.1|CA006891 HU06A02r HU Hordeum vulgare subsp. vulgare cDNA clone HU06A02
5-PRIME, mRNA sequence
Length = 322
Score = 52.0 bits (26), Expect = 4e-005
Identities = 29/30 (96%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacgaagcagaa 414
||||||||| ||||||||||||||||||||
Sbjct: 286 tcacacgaaagaccagttgacgaagcagaa 315
>gb|BI949395.1|BI949395 HVSMEl0013K23f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0013K23f, mRNA sequence
Length = 974
Score = 48.1 bits (24), Expect = 7e-004
Identities = 64/76 (84%), Gaps = 1/76 (1%)
Strand = Plus / Plus
Query: 385 tcacacgaaggaccagttgacg-aagcagaacgccaggcagtggatgttgctatcctcgc 443
||||||||| ||||||||||| ||||| || || |||| ||||| | |||| ||||| |
Sbjct: 390 tcacacgaatgaccagttgacccaagcacaaggcgaggctgtggacgctgctttcctcac 449
Query: 444 cgagcaccttccacgc 459
|||||||||| |||||
Sbjct: 450 cgagcacctttcacgc 465
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 387 acacgaaggaccagttgacgaa 408
||||||||||||||||||||||
Sbjct: 274 acacgaaggaccagttgacgaa 295
>gb|BE411008.1|BE411008 ISC001D05F990323 ITEC ISC Barley Leaf Library Hordeum vulgare
subsp. vulgare cDNA clone ISC001D05, mRNA sequence
Length = 442
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 149 gcagcttcagggaagccagaa 169
>gb|BE411741.1|BE411741 ISC008.G08F990804 ITEC ISC Barley Leaf Library Hordeum vulgare
subsp. vulgare cDNA clone ISC008.G08, mRNA sequence
Length = 438
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 149 gcagcttcagggaagccagaa 169
>gb|BE411855.1|BE411855 ISC010.A02F990902 ITEC ISC Barley Leaf Library Hordeum vulgare
subsp. vulgare cDNA clone ISC010.A02, mRNA sequence
Length = 496
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 149 gcagcttcagggaagccagaa 169
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 387 acacgaaggaccagttgacga 407
|||||||||||||||||||||
Sbjct: 747 acacgaaggaccagttgacga 727
>gb|AV916160.1|AV916160 AV916160 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1n17 5', mRNA sequence
Length = 642
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 176 gcagcttcagggaagccagaa 196
>gb|AV921285.1|AV921285 AV921285 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1n17 3', mRNA sequence
Length = 707
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 536 gcagcttcagggaagccagaa 516
>gb|CB882086.1|CB882086 HM11N04w HM Hordeum vulgare subsp. vulgare cDNA clone HM11N04
3-PRIME, mRNA sequence
Length = 619
Score = 42.1 bits (21), Expect = 0.041
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 821 gcagcttcagggaagccagaa 841
|||||||||||||||||||||
Sbjct: 559 gcagcttcagggaagccagaa 539
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 93,301
Number of Sequences: 312970
Number of extensions: 93301
Number of successful extensions: 23368
Number of sequences better than 0.5: 18
Number of HSP's better than 0.5 without gapping: 17
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 23331
Number of HSP's gapped (non-prelim): 28
length of query: 1190
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1171
effective length of database: 169,188,109
effective search space: 198119275639
effective search space used: 198119275639
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)