BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448489.2.1
(677 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV915249.1|AV915249 AV915249 K. Sato unpublished cDNA li... 240 5e-062
gb|AV921857.1|AV921857 AV921857 K. Sato unpublished cDNA li... 240 5e-062
gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA li... 240 5e-062
gb|BJ459802.1|BJ459802 BJ459802 K. Sato unpublished cDNA li... 176 6e-043
gb|AJ462461.1|AJ462461 AJ462461 S00002 Hordeum vulgare subs... 86 2e-015
gb|CA017134.1|CA017134 HV14M07u HV Hordeum vulgare subsp. v... 76 2e-012
>gb|AV915249.1|AV915249 AV915249 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13i20 5', mRNA sequence
Length = 579
Score = 240 bits (121), Expect = 5e-062
Identities = 283/340 (83%)
Strand = Plus / Minus
Query: 297 ttggccttcatatctaagttctccacatcattaattggtctagttgtctgatccctgaat 356
|||||||||| ||| | ||||||| ||||||||||| | || |||||| |||||
Sbjct: 391 ttggccttcacatcaaggttctccttgtcattaattggctttgtggtctgactcctgatc 332
Query: 357 ctcttgatcttggggctttccagccccttgtgtgagaaaccatatagccgaagcacctgg 416
|||||||||||||||||||||||||||||||||| ||||||||| || || |||||||||
Sbjct: 331 ctcttgatcttggggctttccagccccttgtgtgcgaaaccatacaggcgtagcacctgg 272
Query: 417 ttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccactgggtaatcc 476
|| |||||||||||||| |||| |||||| ||| |||| || || || ||||| |||||
Sbjct: 271 ttatcggcaaaattaaatgctctctccatactcttgagacaccggtcaactggataatca 212
Query: 477 ccatagctcccacatggcttgcacccaacaaagtgagtcacgaaaggccaccgctcgtcg 536
|||||||| || |||||||||||||| |||||||| ||||| || ||||||||||| ||
Sbjct: 211 ccatagcttccgcatggcttgcacccgacaaagtgtgtcacaaatggccaccgctcatcc 152
Query: 537 ccgaggcctnnntggtgattctccatcatctnntcatacttatcnaccnacncagcccag 596
|| || ||| ||||| ||||||||||||| || ||||| || ||| | ||||||||
Sbjct: 151 ccaagccctggatggtggttctccatcatctcctcgtacttgtccaccaatccagcccag 92
Query: 597 aagccatgcnagtagtaggaattctcaatgaacaccttgt 636
|| |||||| ||||||| |||||||| || |||||||||
Sbjct: 91 aaaccatgcaagtagtatgaattctctatatacaccttgt 52
>gb|AV921857.1|AV921857 AV921857 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13i20 3', mRNA sequence
Length = 664
Score = 240 bits (121), Expect = 5e-062
Identities = 283/340 (83%)
Strand = Plus / Plus
Query: 297 ttggccttcatatctaagttctccacatcattaattggtctagttgtctgatccctgaat 356
|||||||||| ||| | ||||||| ||||||||||| | || |||||| |||||
Sbjct: 325 ttggccttcacatcaaggttctccttgtcattaattggctttgtggtctgactcctgatc 384
Query: 357 ctcttgatcttggggctttccagccccttgtgtgagaaaccatatagccgaagcacctgg 416
|||||||||||||||||||||||||||||||||| ||||||||| || || |||||||||
Sbjct: 385 ctcttgatcttggggctttccagccccttgtgtgcgaaaccatacaggcgtagcacctgg 444
Query: 417 ttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccactgggtaatcc 476
|| |||||||||||||| |||| |||||| ||| |||| || || || ||||| |||||
Sbjct: 445 ttatcggcaaaattaaatgctctctccatactcttgagacaccggtcaactggataatca 504
Query: 477 ccatagctcccacatggcttgcacccaacaaagtgagtcacgaaaggccaccgctcgtcg 536
|||||||| || |||||||||||||| |||||||| ||||| || ||||||||||| ||
Sbjct: 505 ccatagcttccgcatggcttgcacccgacaaagtgtgtcacaaatggccaccgctcatcc 564
Query: 537 ccgaggcctnnntggtgattctccatcatctnntcatacttatcnaccnacncagcccag 596
|| || ||| ||||| ||||||||||||| || ||||| || ||| | ||||||||
Sbjct: 565 ccaagccctggatggtggttctccatcatctcctcgtacttgtccaccaatccagcccag 624
Query: 597 aagccatgcnagtagtaggaattctcaatgaacaccttgt 636
|| |||||| ||||||| |||||||| || |||||||||
Sbjct: 625 aaaccatgcaagtagtatgaattctctatatacaccttgt 664
>gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i03 3', mRNA sequence
Length = 653
Score = 240 bits (121), Expect = 5e-062
Identities = 283/340 (83%)
Strand = Plus / Plus
Query: 297 ttggccttcatatctaagttctccacatcattaattggtctagttgtctgatccctgaat 356
|||||||||| ||| | ||||||| ||||||||||| | || |||||| |||||
Sbjct: 215 ttggccttcacatcaaggttctccttgtcattaattggctttgtggtctgactcctgatc 274
Query: 357 ctcttgatcttggggctttccagccccttgtgtgagaaaccatatagccgaagcacctgg 416
|||||||||||||||||||||||||||||||||| ||||||||| || || |||||||||
Sbjct: 275 ctcttgatcttggggctttccagccccttgtgtgcgaaaccatacaggcgtagcacctgg 334
Query: 417 ttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccactgggtaatcc 476
|| |||||||||||||| |||| |||||| ||| |||| || || || ||||| |||||
Sbjct: 335 ttatcggcaaaattaaatgctctctccatactcttgagacaccggtcaactggataatca 394
Query: 477 ccatagctcccacatggcttgcacccaacaaagtgagtcacgaaaggccaccgctcgtcg 536
|||||||| || |||||||||||||| |||||||| ||||| || ||||||||||| ||
Sbjct: 395 ccatagcttccgcatggcttgcacccgacaaagtgtgtcacaaatggccaccgctcatcc 454
Query: 537 ccgaggcctnnntggtgattctccatcatctnntcatacttatcnaccnacncagcccag 596
|| || ||| ||||| ||||||||||||| || ||||| || ||| | ||||||||
Sbjct: 455 ccaagccctggatggtggttctccatcatctcctcgtacttgtccaccaatccagcccag 514
Query: 597 aagccatgcnagtagtaggaattctcaatgaacaccttgt 636
|| |||||| ||||||| |||||||| || |||||||||
Sbjct: 515 aaaccatgcaagtagtatgaattctctatatacaccttgt 554
>gb|BJ459802.1|BJ459802 BJ459802 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38f08 3', mRNA sequence
Length = 606
Score = 176 bits (89), Expect = 6e-043
Identities = 188/221 (85%)
Strand = Plus / Plus
Query: 297 ttggccttcatatctaagttctccacatcattaattggtctagttgtctgatccctgaat 356
|||||||||| ||| | ||||||| ||||||||||| | || |||||| |||||
Sbjct: 386 ttggccttcacatcaaggttctccttgtcattaattggctttgtggtctgactcctgatc 445
Query: 357 ctcttgatcttggggctttccagccccttgtgtgagaaaccatatagccgaagcacctgg 416
|||||||||||||||||||||||||||||||||| ||||||||| || || |||||||||
Sbjct: 446 ctcttgatcttggggctttccagccccttgtgtgcgaaaccatacaggcgtagcacctgg 505
Query: 417 ttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccactgggtaatcc 476
|| |||||||||||||| |||| |||||| ||| |||| || || || ||||| |||||
Sbjct: 506 ttatcggcaaaattaaatgctctctccatactcttgagacaccggtcaactggataatca 565
Query: 477 ccatagctcccacatggcttgcacccaacaaagtgagtcac 517
|||||||| || |||||||||||||| |||||||| |||||
Sbjct: 566 ccatagcttccgcatggcttgcacccgacaaagtgtgtcac 606
>gb|AJ462461.1|AJ462461 AJ462461 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200052A08F1, mRNA sequence
Length = 360
Score = 85.7 bits (43), Expect = 2e-015
Identities = 130/160 (81%)
Strand = Plus / Minus
Query: 408 agcacctggttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccact 467
||||||||||||||||| || || || || |||||||||| ||||||| |||||||
Sbjct: 180 agcacctggttgtcggcgaagttgaaggcgcgctccatgccggtgaggcactgctccacc 121
Query: 468 gggtaatccccatagctcccacatggcttgcacccaacaaagtgagtcacgaaaggccac 527
||||| || || ||||| || || |||||||| || || ||||| || | ||| ||||||
Sbjct: 120 gggtagtcgccgtagctgccgcagggcttgcatccgacgaagtgcgtgatgaagggccac 61
Query: 528 cgctcgtcgccgaggcctnnntggtgattctccatcatct 567
|||||||||||||| || ||||| |||||||||||||
Sbjct: 60 cgctcgtcgccgagccccgggtggtgcttctccatcatct 21
>gb|CA017134.1|CA017134 HV14M07u HV Hordeum vulgare subsp. vulgare cDNA clone HV14M07
3-PRIME, mRNA sequence
Length = 503
Score = 75.8 bits (38), Expect = 2e-012
Identities = 110/134 (82%)
Strand = Plus / Plus
Query: 408 agcacctggttgtcggcaaaattaaacgctcgctccatgctcctgaggcagcgctccact 467
||||||||||||||||| || || || || |||||||||| ||||||| |||||||
Sbjct: 367 agcacctggttgtcggcgaagttgaaggcgcgctccatgccggtgaggcactgctccacc 426
Query: 468 gggtaatccccatagctcccacatggcttgcacccaacaaagtgagtcacgaaaggccac 527
||||| || || ||||| || || |||||||| || || ||||| || | ||| ||||||
Sbjct: 427 gggtagtcgccgtagctgccgcagggcttgcatccgacgaagtgcgtgatgaagggccac 486
Query: 528 cgctcgtcgccgag 541
||||||||||||||
Sbjct: 487 cgctcgtcgccgag 500
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 46,402
Number of Sequences: 312970
Number of extensions: 46402
Number of successful extensions: 11289
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11275
Number of HSP's gapped (non-prelim): 14
length of query: 677
length of database: 175,134,539
effective HSP length: 19
effective length of query: 658
effective length of database: 169,188,109
effective search space: 111325775722
effective search space used: 111325775722
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)