BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430991.2.1
(515 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgar... 432 e-120
gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA li... 244 2e-063
gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA li... 242 9e-063
gb|BQ471670.1|BQ471670 HV03B24r HV Hordeum vulgare subsp. v... 236 6e-061
gb|AL450785.1|AL450785 AL450785 Hordeum vulgare Barke etiol... 123 6e-027
gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day ger... 48 3e-004
gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day ger... 48 3e-004
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 48 3e-004
>gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P119 5-PRIME, mRNA sequence
Length = 787
Score = 432 bits (218), Expect = e-120
Identities = 397/457 (86%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccagagaagtagaact 60
||||||| || ||||| || ||||||||||||||| ||||||| |||||||||||||| |
Sbjct: 463 actttggatttctgaacttgattggtgttccacgattaccacttccagagaagtagaaat 404
Query: 61 tctgtgtagcagcattctgtagctgtttaagaacgggaacatacgatgcattgagccagg 120
||||||||||||||| ||| |||||||||||||| |||||||| || ||||| |||||||
Sbjct: 403 tctgtgtagcagcatcctggagctgtttaagaacaggaacataggaagcatttagccagg 344
Query: 121 taaagtcctccaccttttgtatctccacagtgacccctctgtagtcattcatgccaaacc 180
| |||||||||||||| |||||||||||| || |||||||||||||||||| | ||||||
Sbjct: 343 tgaagtcctccaccttctgtatctccacaatggcccctctgtagtcattcaaggcaaacc 284
Query: 181 atgccttcatcggagcatagttcaactcatcagtcactaagtgaaaaacaaccttgtcag 240
| ||| |||| || ||||||| | ||| || ||||| || |||||||||| ||| ||||
Sbjct: 283 acgccctcattgggtcatagttgacctcgtcggtcaccaaatgaaaaacaatcttttcag 224
Query: 241 ggtgcctcgagtttatagccgtagaattaacaacaactgaaacagcaaggatgttgtctg 300
||||| | || || | || |||||||| | ||||| || ||||||| ||||||||| |
Sbjct: 223 ggtgctttgaattcagagttgtagaattgatgacaacagagacagcaatgatgttgtccg 164
Query: 301 agaagacacaaaaatgatagaggctgttatccctcagattgctctgcactgctgggctcc 360
|||| ||||| | ||||| ||||| |||||||||||||||||||||||||| |||||||
Sbjct: 163 agaaaacacagtagtgataaaggctattatccctcagattgctctgcactgcagggctcc 104
Query: 361 tatccgaaaacttcctctgaagctcgggactcttgaaccantccatggttaaccggacac 420
||||| | ||||||||||||||||||| ||| |||||||| |||||||||||||||||||
Sbjct: 103 tatccaagaacttcctctgaagctcggcactgttgaaccattccatggttaaccggacac 44
Query: 421 caaggcaataaagtccctttgggagctcctcagcagc 457
||||||||||||| ||||||||||||||||| |||||
Sbjct: 43 caaggcaataaagcccctttgggagctcctcggcagc 7
>gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g14 5', mRNA sequence
Length = 500
Score = 244 bits (123), Expect = 2e-063
Identities = 263/310 (84%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccagagaagtagaact 60
||||||| || ||||| || ||||||||||||||| ||||||| |||||||||||||| |
Sbjct: 317 actttggatttctgaacttgattggtgttccacgattaccacttccagagaagtagaaat 258
Query: 61 tctgtgtagcagcattctgtagctgtttaagaacgggaacatacgatgcattgagccagg 120
||||||||||||||| ||| |||||||||||||| |||||||| || ||||| |||||||
Sbjct: 257 tctgtgtagcagcatcctggagctgtttaagaacaggaacataggaagcatttagccagg 198
Query: 121 taaagtcctccaccttttgtatctccacagtgacccctctgtagtcattcatgccaaacc 180
| |||||||||||||| |||||||||||| || |||||||||||||||||| | ||||||
Sbjct: 197 tgaagtcctccaccttctgtatctccacaatggcccctctgtagtcattcaaggcaaacc 138
Query: 181 atgccttcatcggagcatagttcaactcatcagtcactaagtgaaaaacaaccttgtcag 240
| ||| |||| || ||||||| | ||| || ||||| || |||||||||| ||| ||||
Sbjct: 137 acgccctcattgggtcatagttgacctcgtcggtcaccaaatgaaaaacaatcttttcag 78
Query: 241 ggtgcctcgagtttatagccgtagaattaacaacaactgaaacagcaaggatgttgtctg 300
||||| | || || | || |||||||| | ||||| || ||||||| ||||||||| |
Sbjct: 77 ggtgctttgaattcagagttgtagaattgangacaacagagacagcaatgatgttgtccg 18
Query: 301 agaagacaca 310
|||| |||||
Sbjct: 17 agaaaacaca 8
>gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 5', mRNA sequence
Length = 596
Score = 242 bits (122), Expect = 9e-063
Identities = 263/310 (84%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccagagaagtagaact 60
||||||| || ||||| || ||||||||||||||| ||||||| |||||||||||||| |
Sbjct: 318 actttggatttctgaacttgattggtgttccacgattaccacttccagagaagtagaaat 259
Query: 61 tctgtgtagcagcattctgtagctgtttaagaacgggaacatacgatgcattgagccagg 120
||||||||||||||| ||| |||||||||||||| |||||||| || ||||| |||||||
Sbjct: 258 tctgtgtagcagcatcctggagctgtttaagaacaggaacataggaagcatttagccagg 199
Query: 121 taaagtcctccaccttttgtatctccacagtgacccctctgtagtcattcatgccaaacc 180
| |||||||||||||| |||||||||||| || |||||||||||||||||| | ||||||
Sbjct: 198 tgaagtcctccaccttctgtatctccacaatggcccctctgtagtcattcaaggcaaacc 139
Query: 181 atgccttcatcggagcatagttcaactcatcagtcactaagtgaaaaacaaccttgtcag 240
| ||| |||| || ||||||| | ||| || ||||| || |||||||||| ||| ||||
Sbjct: 138 acgccctcattgggtcatagttgacctcgtcggtcaccaaatgaaaaacaatcttttcag 79
Query: 241 ggtgcctcgagtttatagccgtagaattaacaacaactgaaacagcaaggatgttgtctg 300
||||| | || || | || |||||||| | ||||| || ||||||| ||||||||| |
Sbjct: 78 ggtgctttgaattcagagttgtagaattgatgacaacagagacagcaatgatgttgtccg 19
Query: 301 agaagacaca 310
|||| |||||
Sbjct: 18 agaaaacaca 9
>gb|BQ471670.1|BQ471670 HV03B24r HV Hordeum vulgare subsp. vulgare cDNA clone HV03B24
5-PRIME, mRNA sequence
Length = 403
Score = 236 bits (119), Expect = 6e-061
Identities = 226/262 (86%)
Strand = Plus / Minus
Query: 196 catagttcaactcatcagtcactaagtgaaaaacaaccttgtcagggtgcctcgagttta 255
||||||| | ||| || ||||| || |||||||||| ||| ||||||||| | || || |
Sbjct: 399 catagttgacctcgtcggtcaccaaatgaaaaacaatcttttcagggtgctttgaattca 340
Query: 256 tagccgtagaattaacaacaactgaaacagcaaggatgttgtctgagaagacacaaaaat 315
|| |||||||| | ||||| || ||||||| ||||||||| ||||| ||||| | |
Sbjct: 339 gagttgtagaattgatgacaacagagacagcaatgatgttgtccgagaaaacacagtagt 280
Query: 316 gatagaggctgttatccctcagattgctctgcactgctgggctcctatccgaaaacttcc 375
|||| ||||| |||||||||||||||||||||||||| |||||||||||| | |||||||
Sbjct: 279 gataaaggctattatccctcagattgctctgcactgcagggctcctatccaagaacttcc 220
Query: 376 tctgaagctcgggactcttgaaccantccatggttaaccggacaccaaggcaataaagtc 435
|||||||||||| ||| |||||||| |||||||||||||||||||||||||||||||| |
Sbjct: 219 tctgaagctcggcactgttgaaccattccatggttaaccggacaccaaggcaataaagcc 160
Query: 436 cctttgggagctcctcagcagc 457
|||||||||||||||| |||||
Sbjct: 159 cctttgggagctcctcggcagc 138
>gb|AL450785.1|AL450785 AL450785 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04D14u 5', mRNA sequence
Length = 296
Score = 123 bits (62), Expect = 6e-027
Identities = 236/292 (80%), Gaps = 3/292 (1%)
Strand = Plus / Minus
Query: 60 ttctgtgtag-cagcattctgtagctgtttaagaacgggaacatacgatgcattgagcca 118
|||||||||| |||||| || |||||||||||||| |||||||| || ||||| | |||
Sbjct: 292 ttctgtgtaggcagcatcntggagctgtttaagaacaggaacataggaagcatttaacca 233
Query: 119 ggtaaagtcc-tccaccttttgtatctcc-acagtgacccctctgtagtcattcatgcca 176
||| ||||| |||||||| ||||| ||| ||| || ||||||| |||||||||| | ||
Sbjct: 232 ggtgaagtcnatccaccttctgtatttcccacaatggcccctctntagtcattcaaggca 173
Query: 177 aaccatgccttcatcggagcatagttcaactcatcagtcactaagtgaaaaacaaccttg 236
||||| ||| |||| || ||||||| | ||| || ||||| || |||||||||| |||
Sbjct: 172 aaccacgccctcattgggtcatagttgacctcgtcggtcaccaaatgaaaaacaatcttt 113
Query: 237 tcagggtgcctcgagtttatagccgtagaattaacaacaactgaaacagcaaggatgttg 296
||||||||| | || || | || |||||||| | ||||| || |||||| |||||||
Sbjct: 112 tcagggtgctttgaattcagagttgtagaattgatgacaacagagacagcantgatgttg 53
Query: 297 tctgagaagacacaaaaatgatagaggctgttatccctcagattgctctgca 348
| ||||| ||||| | ||||| ||||| ||||||||||||||||| ||||
Sbjct: 52 nccgagaaaacacagtagtgataaaggctattatccctcagattgctntgca 1
>gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_D08 5', mRNA sequence
Length = 464
Score = 48.1 bits (24), Expect = 3e-004
Identities = 42/48 (87%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccag 48
||||||| || ||||| || ||||||||||||||| ||||||| ||||
Sbjct: 48 actttggatttctgaacttgattggtgttccacgattaccacttccag 1
>gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ006_P20 5', mRNA sequence
Length = 417
Score = 48.1 bits (24), Expect = 3e-004
Identities = 42/48 (87%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccag 48
||||||| || ||||| || ||||||||||||||| ||||||| ||||
Sbjct: 48 actttggatttctgaacttgattggtgttccacgattaccacttccag 1
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 48.1 bits (24), Expect = 3e-004
Identities = 42/48 (87%)
Strand = Plus / Minus
Query: 1 actttgggttcctgaatttaattggtgttccacgactaccactgccag 48
||||||| || ||||| || ||||||||||||||| ||||||| ||||
Sbjct: 48 actttggatttctgaacttgattggtgttccacgattaccacttccag 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 40,519
Number of Sequences: 312970
Number of extensions: 40519
Number of successful extensions: 10321
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 10311
Number of HSP's gapped (non-prelim): 8
length of query: 515
length of database: 175,134,539
effective HSP length: 18
effective length of query: 497
effective length of database: 169,501,079
effective search space: 84242036263
effective search space used: 84242036263
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)