BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404858.2.1
(958 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF255425.2|BF255425 HVSMEf0006N05f Hordeum vulgare seedl... 238 3e-061
gb|BG299290.1|BG299290 HVSMEa0019D17f Hordeum vulgare seedl... 163 1e-038
gb|CB859379.1|CB859379 HI10E13w HI Hordeum vulgare subsp. v... 62 4e-008
gb|BI960545.1|BI960545 HVSMEn0024P18f Hordeum vulgare rachi... 46 0.002
gb|BI960547.1|BI960547 HVSMEn0024P20f Hordeum vulgare rachi... 46 0.002
gb|BF617721.3|BF617721 HVSMEc0018O08f Hordeum vulgare seedl... 42 0.033
gb|BF629976.2|BF629976 HVSMEb0007I10f Hordeum vulgare seedl... 40 0.13
gb|BF618753.2|BF618753 HVSMEc0007K17f Hordeum vulgare seedl... 40 0.13
gb|BE216488.1|BE216488 HV_CEb0010K02f Hordeum vulgare seedl... 40 0.13
gb|BI959799.1|BI959799 HVSMEn0021J14f Hordeum vulgare rachi... 40 0.13
gb|BE196606.3|BE196606 HVSMEh0092M06f Hordeum vulgare 5-45 ... 40 0.13
gb|BQ462899.1|BQ462899 HI02F20r HI Hordeum vulgare subsp. v... 40 0.13
gb|BQ463044.1|BQ463044 HI02N17r HI Hordeum vulgare subsp. v... 40 0.13
gb|BM372991.2|BM372991 EBma04_SQ002_K15_R maternal, 10 DPA,... 40 0.13
gb|BU997747.1|BU997747 HI08O09r HI Hordeum vulgare subsp. v... 40 0.13
gb|BU999748.1|BU999748 HI15K05r HI Hordeum vulgare subsp. v... 40 0.13
gb|CA020535.1|CA020535 HZ36J17r HZ Hordeum vulgare subsp. v... 40 0.13
gb|CA020976.1|CA020976 HZ38K15r HZ Hordeum vulgare subsp. v... 40 0.13
gb|CA021212.1|CA021212 HZ39H04r HZ Hordeum vulgare subsp. v... 40 0.13
gb|CA025777.1|CA025777 HZ53B20r HZ Hordeum vulgare subsp. v... 40 0.13
gb|CA027713.1|CA027713 HZ59M14r HZ Hordeum vulgare subsp. v... 40 0.13
>gb|BF255425.2|BF255425 HVSMEf0006N05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006N05f, mRNA sequence
Length = 587
Score = 238 bits (120), Expect = 3e-061
Identities = 350/426 (82%), Gaps = 3/426 (0%)
Strand = Plus / Plus
Query: 5 tctgcgccgatctcggcgcgggtgccgccagctacgtccactgcgacgtcaccagcgagg 64
||||||| || |||||| ||| ||| |||||||||| | |||||||||||||||||||
Sbjct: 162 tctgcgcggagctcggcccggcggcctccagctacgtgcggtgcgacgtcaccagcgagg 221
Query: 65 acgacgtcgcggccgcggtggaccacgccgtggcgacgttcggcgcgctggacatcatgt 124
| ||||||||||| || || || ||| |||| || | |||||| ||||||||||||||
Sbjct: 222 aggacgtcgcggcggccgtcgatcacaccgtcgccaggttcgggaagctggacatcatgt 281
Query: 125 tcaacaacgcgggcgtcagcgggccggcgtgctacagcgtccgggagagcggcaaggctg 184
|||||||||| ||| || |||| |||||||| ||||| ||||||| ||| ||||| |
Sbjct: 282 tcaacaacgccggcatcggcggcgcggcgtgccacagcatccgggacagcaccaaggagg 341
Query: 185 acttcgagcgcgtgctggccgtgaatctggtgggcccgttcctcggcaccaagcacgcgg 244
|||||||||||||||||||||| || || ||||| || ||||| || |||||||||||||
Sbjct: 342 acttcgagcgcgtgctggccgtcaacctcgtggggcccttcctgggtaccaagcacgcgg 401
Query: 245 cgcgggtgatggtgccctcgcgccgcggtggctgcatcatagggacgtcgagcgtggcgg 304
||||||| ||||||||| |||| | ||||||||||| |||||||||||| |||||
Sbjct: 402 cgcgggtcatggtgcccgcgcggc---aaggctgcatcatcgggacgtcgagcctggcgt 458
Query: 305 cggcagtgactggcgtgtcgtcgtacgcgtacgcgtgcgcgaagcgcgcgatggtgactc 364
|||| ||| |||||| ||||| |||| ||| ||||||| |||||||| |||| |
Sbjct: 459 cggcggtgggcggcgtggcgtcgcacgcctacacgtgcgccaagcgcgccctggtcgggc 518
Query: 365 tgacggagaacgcggctgcggagctcggccagcacggcattcgcgtgaactgcgtctccc 424
||||||| |||||||| ||||||| |||||||| || || ||||| |||||| ||||||
Sbjct: 519 tgacggaaaacgcggcgacggagcttggccagcatgggatccgcgtcaactgcatctccc 578
Query: 425 ccgcgg 430
| ||||
Sbjct: 579 cggcgg 584
>gb|BG299290.1|BG299290 HVSMEa0019D17f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0019D17f, mRNA sequence
Length = 627
Score = 163 bits (82), Expect = 1e-038
Identities = 217/262 (82%)
Strand = Plus / Plus
Query: 5 tctgcgccgatctcggcgcgggtgccgccagctacgtccactgcgacgtcaccagcgagg 64
||||||| || |||||| ||| ||| |||||||||| | |||||| ||||||||||||
Sbjct: 181 tctgcgcggagctcggcccggcggcctccagctacgtgcggtgcgacttcaccagcgagg 240
Query: 65 acgacgtcgcggccgcggtggaccacgccgtggcgacgttcggcgcgctggacatcatgt 124
| ||| || |||| || || | |||||||| || | |||||| ||||||||||||||
Sbjct: 241 aggacatcacggcggccgtctatcacgccgtcgccaggttcgggaagctggacatcatgt 300
Query: 125 tcaacaacgcgggcgtcagcgggccggcgtgctacagcgtccgggagagcggcaaggctg 184
|||||||||| ||| || |||| |||||||| ||||| ||||||| ||| ||||| |
Sbjct: 301 tcaacaacgccggcatcggcggcgcggcgtgccacagcttccgggacagcaccaaggagg 360
Query: 185 acttcgagcgcgtgctggccgtgaatctggtgggcccgttcctcggcaccaagcacgcgg 244
|||||||||||||||||||||| || || ||||| || ||||| || |||||||||||||
Sbjct: 361 acttcgagcgcgtgctggccgtcaacctcgtggggcccttcctgggtaccaagcacgcgg 420
Query: 245 cgcgggtgatggtgccctcgcg 266
||||||| ||||||||| ||||
Sbjct: 421 cgcgggtcatggtgcccgcgcg 442
>gb|CB859379.1|CB859379 HI10E13w HI Hordeum vulgare subsp. vulgare cDNA clone HI10E13
3-PRIME, mRNA sequence
Length = 488
Score = 61.9 bits (31), Expect = 4e-008
Identities = 52/59 (88%)
Strand = Plus / Minus
Query: 562 ttcctggccagcgatgacgcgaggtacatcagcggccacaacctgttcgtcgacggcgg 620
|||||||||||||| || ||||||||||| |||||||||||| |||||||||||||
Sbjct: 483 ttcctggccagcgacgagtcgaggtacatctccggccacaacctcgtcgtcgacggcgg 425
>gb|BI960545.1|BI960545 HVSMEn0024P18f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024P18f, mRNA sequence
Length = 658
Score = 46.1 bits (23), Expect = 0.002
Identities = 83/103 (80%)
Strand = Plus / Plus
Query: 39 cgtccactgcgacgtcaccagcgaggacgacgtcgcggccgcggtggaccacgccgtggc 98
|||||||||||||||| | ||||||| ||||||| | || |||||| |||||| ||
Sbjct: 250 cgtccactgcgacgtcgtccacgaggacaacgtcgccggggcagtggacgccgccgtcgc 309
Query: 99 gacgttcggcgcgctggacatcatgttcaacaacgcgggcgtc 141
|| | |||| ||||||| |||||||||||| ||| ||||||
Sbjct: 310 gaggcacggccggctggacgtcatgttcaacagcgccggcgtc 352
>gb|BI960547.1|BI960547 HVSMEn0024P20f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024P20f, mRNA sequence
Length = 655
Score = 46.1 bits (23), Expect = 0.002
Identities = 83/103 (80%)
Strand = Plus / Plus
Query: 39 cgtccactgcgacgtcaccagcgaggacgacgtcgcggccgcggtggaccacgccgtggc 98
|||||||||||||||| | ||||||| ||||||| | || |||||| |||||| ||
Sbjct: 250 cgtccactgcgacgtcgtccacgaggacaacgtcgccggggcagtggacgccgccgtcgc 309
Query: 99 gacgttcggcgcgctggacatcatgttcaacaacgcgggcgtc 141
|| | |||| ||||||| |||||||||||| ||| ||||||
Sbjct: 310 gaggcacggccggctggacgtcatgttcaacagcgccggcgtc 352
>gb|BF617721.3|BF617721 HVSMEc0018O08f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0018O08f, mRNA sequence
Length = 790
Score = 42.1 bits (21), Expect = 0.033
Identities = 54/65 (83%)
Strand = Plus / Plus
Query: 70 gtcgcggccgcggtggaccacgccgtggcgacgttcggcgcgctggacatcatgttcaac 129
||||| || |||||||||| ||||||||| | |||| ||||||||||||| |||||
Sbjct: 343 gtcgccgcggcggtggacctcgccgtggcccggcacggcaagctggacatcatgctcaac 402
Query: 130 aacgc 134
|||||
Sbjct: 403 aacgc 407
>gb|BF629976.2|BF629976 HVSMEb0007I10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0007I10f, mRNA sequence
Length = 498
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 387 gctggacatcatgctcaacaacgc 410
>gb|BF618753.2|BF618753 HVSMEc0007K17f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0007K17f, mRNA sequence
Length = 796
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 13 gctggacatcatgctcaacaacgc 36
>gb|BE216488.1|BE216488 HV_CEb0010K02f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0010K02f, mRNA sequence
Length = 747
Score = 40.1 bits (20), Expect = 0.13
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 848 ggttttctaaactaaaaata 867
||||||||||||||||||||
Sbjct: 522 ggttttctaaactaaaaata 541
>gb|BI959799.1|BI959799 HVSMEn0021J14f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021J14f, mRNA sequence
Length = 654
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 370 gctggacatcatgctcaacaacgc 393
>gb|BE196606.3|BE196606 HVSMEh0092M06f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0092M06f, mRNA sequence
Length = 921
Score = 40.1 bits (20), Expect = 0.13
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 165 ccgggagagcggcaaggctg 184
||||||||||||||||||||
Sbjct: 900 ccgggagagcggcaaggctg 919
>gb|BQ462899.1|BQ462899 HI02F20r HI Hordeum vulgare subsp. vulgare cDNA clone HI02F20
5-PRIME, mRNA sequence
Length = 454
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 397 gctggacatcatgctcaacaacgc 420
>gb|BQ463044.1|BQ463044 HI02N17r HI Hordeum vulgare subsp. vulgare cDNA clone HI02N17
5-PRIME, mRNA sequence
Length = 357
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 253 gctggacatcatgctcaacaacgc 276
>gb|BM372991.2|BM372991 EBma04_SQ002_K15_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ002_K15 5', mRNA sequence
Length = 615
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 382 gctggacatcatgctcaacaacgc 405
>gb|BU997747.1|BU997747 HI08O09r HI Hordeum vulgare subsp. vulgare cDNA clone HI08O09
5-PRIME, mRNA sequence
Length = 622
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 392 gctggacatcatgctcaacaacgc 415
>gb|BU999748.1|BU999748 HI15K05r HI Hordeum vulgare subsp. vulgare cDNA clone HI15K05
5-PRIME, mRNA sequence
Length = 569
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 392 gctggacatcatgctcaacaacgc 415
>gb|CA020535.1|CA020535 HZ36J17r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ36J17
5-PRIME, mRNA sequence
Length = 556
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 168 gctggacatcatgctcaacaacgc 191
>gb|CA020976.1|CA020976 HZ38K15r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ38K15
5-PRIME, mRNA sequence
Length = 440
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 220 gctggacatcatgctcaacaacgc 243
>gb|CA021212.1|CA021212 HZ39H04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ39H04
5-PRIME, mRNA sequence
Length = 427
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 351 gctggacatcatgctcaacaacgc 374
>gb|CA025777.1|CA025777 HZ53B20r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ53B20
5-PRIME, mRNA sequence
Length = 407
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 140 gctggacatcatgctcaacaacgc 163
>gb|CA027713.1|CA027713 HZ59M14r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59M14
5-PRIME, mRNA sequence
Length = 333
Score = 40.1 bits (20), Expect = 0.13
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 111 gctggacatcatgttcaacaacgc 134
||||||||||||| ||||||||||
Sbjct: 179 gctggacatcatgctcaacaacgc 202
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 156,975
Number of Sequences: 312970
Number of extensions: 156975
Number of successful extensions: 42874
Number of sequences better than 0.5: 21
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 42835
Number of HSP's gapped (non-prelim): 39
length of query: 958
length of database: 175,134,539
effective HSP length: 19
effective length of query: 939
effective length of database: 169,188,109
effective search space: 158867634351
effective search space used: 158867634351
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)