BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404725.2.9
(1195 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow st... 285 2e-075
gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow st... 278 4e-073
gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. v... 234 5e-060
gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm libra... 82 5e-014
gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm librar... 82 5e-014
gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm librar... 82 5e-014
gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm librar... 82 5e-014
gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm libra... 82 5e-014
gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots... 44 0.011
gb|BQ663359.1|BQ663359 HU02F19u HU Hordeum vulgare subsp. v... 44 0.011
gb|BQ765944.1|BQ765944 EBro03_SQ008_I14_R root, 3 week, wat... 44 0.011
gb|CA005968.1|CA005968 HU05L22u HU Hordeum vulgare subsp. v... 44 0.011
>gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_D11 5', mRNA sequence
Length = 676
Score = 285 bits (144), Expect = 2e-075
Identities = 219/244 (89%)
Strand = Plus / Plus
Query: 4 caaccagtgcaggtccttccgctggaacctgacggccagcgcggcgcggcccaacccgca 63
||||||||| |||||||||||||||||||||||||||||||| || |||||||||||||
Sbjct: 301 caaccagtggaggtccttccgctggaacctgacggccagcgccgccaggcccaacccgca 360
Query: 64 ggggtcgtaccactacggccagatcaacatcacccgcaccatcaagctcgccatgggccg 123
|||||| |||||||||||||||||||||||||||||||||||||||||| ||| ||||
Sbjct: 361 ggggtcctaccactacggccagatcaacatcacccgcaccatcaagctctccaccagccg 420
Query: 124 cggcaaggtcgacggcaaggagaggttcgccttcaacggcgtgtcgcacgtggacccgga 183
|||||||||||||||||||||||||| |||| |||||||||| || |||| ||| | ||
Sbjct: 421 cggcaaggtcgacggcaaggagaggtacgccctcaacggcgtctcccacgccgacgccga 480
Query: 184 aacccccgtcaagctcgccgagtacttcaacgccagcgacggcgtgttcaagtacaacct 243
|| ||| ||||||||||||||||||||||||||| | | || |||||| ||||||||||
Sbjct: 481 gacgcccctcaagctcgccgagtacttcaacgccaccaagggagtgttcgagtacaacct 540
Query: 244 catc 247
||||
Sbjct: 541 catc 544
Score = 81.8 bits (41), Expect = 5e-014
Identities = 80/93 (86%)
Strand = Plus / Plus
Query: 287 gcccccaacgtcatccgcgccgagttccgcaccttcatcgaggtggtcttcgagaacccc 346
||||||||||||||| ||||||| |||||||| ||| || || ||||| |||||||||
Sbjct: 584 gcccccaacgtcatcaccgccgagcaccgcacctacattgaagtcgtctttgagaacccc 643
Query: 347 gagaagagcatcgacaccatccacatcgacggc 379
|||| ||||||||||||| ||||| || |||||
Sbjct: 644 gagaggagcatcgacaccttccacctcaacggc 676
>gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G14 5', mRNA sequence
Length = 341
Score = 278 bits (140), Expect = 4e-073
Identities = 218/244 (89%)
Strand = Plus / Plus
Query: 4 caaccagtgcaggtccttccgctggaacctgacggccagcgcggcgcggcccaacccgca 63
||||||||| |||||||||||||||||||||||||||||||| || |||||||||||||
Sbjct: 82 caaccagtggaggtccttccgctggaacctgacggccagcgccgccaggcccaacccgca 141
Query: 64 ggggtcgtaccactacggccagatcaacatcacccgcaccatcaagctcgccatgggccg 123
|||||| |||||||||||||||||||||||||||||||||||||||||| ||| ||||
Sbjct: 142 ggggtcctaccactacggccagatcaacatcacccgcaccatcaagctctccaccagccg 201
Query: 124 cggcaaggtcgacggcaaggagaggttcgccttcaacggcgtgtcgcacgtggacccgga 183
|||||||||||||||||||||||||| |||| |||||||||| | |||| ||| | ||
Sbjct: 202 cggcaaggtcgacggcaaggagaggtacgccctcaacggcgtcttccacgccgacgccga 261
Query: 184 aacccccgtcaagctcgccgagtacttcaacgccagcgacggcgtgttcaagtacaacct 243
|| ||| ||||||||||||||||||||||||||| | | || |||||| ||||||||||
Sbjct: 262 gacgcccctcaagctcgccgagtacttcaacgccaccaagggagtgttcgagtacaacct 321
Query: 244 catc 247
||||
Sbjct: 322 catc 325
>gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. vulgare cDNA clone HM14F23
5-PRIME, mRNA sequence
Length = 628
Score = 234 bits (118), Expect = 5e-060
Identities = 151/162 (93%)
Strand = Plus / Plus
Query: 4 caaccagtgcaggtccttccgctggaacctgacggccagcgcggcgcggcccaacccgca 63
||||||||| |||||||||||||||||||||||||||||||| || |||||||||||||
Sbjct: 442 caaccagtggaggtccttccgctggaacctgacggccagcgccgccaggcccaacccgca 501
Query: 64 ggggtcgtaccactacggccagatcaacatcacccgcaccatcaagctcgccatgggccg 123
|||||| |||||||||||||||||||||||||||||||||||||||||| ||| ||||
Sbjct: 502 ggggtcctaccactacggccagatcaacatcacccgcaccatcaagctctccaccagccg 561
Query: 124 cggcaaggtcgacggcaaggagaggttcgccttcaacggcgt 165
|||||||||||||||||||||||||| |||| ||||||||||
Sbjct: 562 cggcaaggtcgacggcaaggagaggtacgccctcaacggcgt 603
>gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL117e7 5' similar to Oryza sativa,
mRNA sequence
Length = 674
Score = 81.8 bits (41), Expect = 5e-014
Identities = 77/89 (86%)
Strand = Plus / Plus
Query: 28 gaacctgacggccagcgcggcgcggcccaacccgcaggggtcgtaccactacggccagat 87
|||||||||||| |||| | ||||||| ||||||||||| ||||||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 88 caacatcacccgcaccatcaagctcgcca 116
|||| | | ||||||||||| ||| ||||
Sbjct: 271 caacgtgagccgcaccatcaggctggcca 299
>gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL37h7 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 568
Score = 81.8 bits (41), Expect = 5e-014
Identities = 77/89 (86%)
Strand = Plus / Plus
Query: 28 gaacctgacggccagcgcggcgcggcccaacccgcaggggtcgtaccactacggccagat 87
|||||||||||| |||| | ||||||| ||||||||||| ||||||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 88 caacatcacccgcaccatcaagctcgcca 116
|||| | | ||||||||||| ||| ||||
Sbjct: 271 caacgtgagccgcaccatcaggctggcca 299
>gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL49f3 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 502
Score = 81.8 bits (41), Expect = 5e-014
Identities = 77/89 (86%)
Strand = Plus / Plus
Query: 28 gaacctgacggccagcgcggcgcggcccaacccgcaggggtcgtaccactacggccagat 87
|||||||||||| |||| | ||||||| ||||||||||| ||||||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 88 caacatcacccgcaccatcaagctcgcca 116
|||| | | ||||||||||| ||| ||||
Sbjct: 271 caacgtgagccgcaccatcaggctggcca 299
>gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL82f4 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 581
Score = 81.8 bits (41), Expect = 5e-014
Identities = 77/89 (86%)
Strand = Plus / Plus
Query: 28 gaacctgacggccagcgcggcgcggcccaacccgcaggggtcgtaccactacggccagat 87
|||||||||||| |||| | ||||||| ||||||||||| ||||||||||||||| |||
Sbjct: 483 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 542
Query: 88 caacatcacccgcaccatcaagctcgcca 116
|||| | | ||||||||||| ||| ||||
Sbjct: 543 caacgtgagccgcaccatcaggctggcca 571
>gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL89d11 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 593
Score = 81.8 bits (41), Expect = 5e-014
Identities = 77/89 (86%)
Strand = Plus / Plus
Query: 28 gaacctgacggccagcgcggcgcggcccaacccgcaggggtcgtaccactacggccagat 87
|||||||||||| |||| | ||||||| ||||||||||| ||||||||||||||| |||
Sbjct: 211 gaacctgacggcgagcgggccgcggccgaacccgcagggctcgtaccactacggctcgat 270
Query: 88 caacatcacccgcaccatcaagctcgcca 116
|||| | | ||||||||||| ||| ||||
Sbjct: 271 caacgtgagccgcaccatcaggctggcca 299
>gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06V 5', mRNA sequence
Length = 694
Score = 44.1 bits (22), Expect = 0.011
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 368 cacatcgacggctacgccttct 389
||||||||||||||||||||||
Sbjct: 132 cacatcgacggctacgccttct 153
>gb|BQ663359.1|BQ663359 HU02F19u HU Hordeum vulgare subsp. vulgare cDNA clone HU02F19
3-PRIME, mRNA sequence
Length = 354
Score = 44.1 bits (22), Expect = 0.011
Identities = 25/26 (96%)
Strand = Plus / Minus
Query: 32 ctgacggccagcgcggcgcggcccaa 57
|||||||||||||||||| |||||||
Sbjct: 276 ctgacggccagcgcggcgaggcccaa 251
>gb|BQ765944.1|BQ765944 EBro03_SQ008_I14_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_I14 5', mRNA sequence
Length = 417
Score = 44.1 bits (22), Expect = 0.011
Identities = 25/26 (96%)
Strand = Plus / Plus
Query: 32 ctgacggccagcgcggcgcggcccaa 57
|||||||||||||||||| |||||||
Sbjct: 181 ctgacggccagcgcggcgaggcccaa 206
>gb|CA005968.1|CA005968 HU05L22u HU Hordeum vulgare subsp. vulgare cDNA clone HU05L22
3-PRIME, mRNA sequence
Length = 452
Score = 44.1 bits (22), Expect = 0.011
Identities = 25/26 (96%)
Strand = Plus / Minus
Query: 32 ctgacggccagcgcggcgcggcccaa 57
|||||||||||||||||| |||||||
Sbjct: 276 ctgacggccagcgcggcgaggcccaa 251
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 141,668
Number of Sequences: 312970
Number of extensions: 141668
Number of successful extensions: 39689
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 39666
Number of HSP's gapped (non-prelim): 22
length of query: 1195
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1176
effective length of database: 169,188,109
effective search space: 198965216184
effective search space used: 198965216184
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)