BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192481.2.2
(1053 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621527.1|BF621527 HVSMEa0011E23f Hordeum vulgare seedl... 48 6e-004
gb|BF621761.2|BF621761 HVSMEa0012H13f Hordeum vulgare seedl... 48 6e-004
gb|BF629242.2|BF629242 HVSMEb0010H15f Hordeum vulgare seedl... 48 6e-004
gb|BG300031.1|BG300031 HVSMEb0011B11f Hordeum vulgare seedl... 48 6e-004
gb|BF254292.2|BF254292 HVSMEf0003J01f Hordeum vulgare seedl... 48 6e-004
gb|BF258180.2|BF258180 HVSMEf0015A11f Hordeum vulgare seedl... 48 6e-004
gb|BF260055.2|BF260055 HVSMEf0020P20f Hordeum vulgare seedl... 48 6e-004
gb|BE214264.2|BE214264 HV_CEb0002O24f Hordeum vulgare seedl... 48 6e-004
gb|AV932136.1|AV932136 AV932136 K. Sato unpublished cDNA li... 48 6e-004
gb|AV932602.1|AV932602 AV932602 K. Sato unpublished cDNA li... 48 6e-004
gb|AV932651.1|AV932651 AV932651 K. Sato unpublished cDNA li... 48 6e-004
gb|BJ472055.1|BJ472055 BJ472055 K. Sato unpublished cDNA li... 48 6e-004
gb|BQ467934.1|BQ467934 HR01G10r HR Hordeum vulgare subsp. v... 48 6e-004
gb|CA020312.1|CA020312 HV14O23r HV Hordeum vulgare subsp. v... 48 6e-004
gb|BF630969.1|BF630969 HVSMEb0014J08f Hordeum vulgare seedl... 44 0.009
gb|BI956766.1|BI956766 HVSMEn0005D23f Hordeum vulgare rachi... 44 0.009
gb|BI958524.1|BI958524 HVSMEn0015H06f Hordeum vulgare rachi... 44 0.009
gb|BF616863.3|BF616863 HVSMEc0013C20f Hordeum vulgare seedl... 44 0.009
gb|BE519613.3|BE519613 HV_CEb0016F21f Hordeum vulgare seedl... 44 0.009
gb|BU995967.1|BU995967 HM11O24r HM Hordeum vulgare subsp. v... 42 0.037
gb|BF624166.1|BF624166 HVSMEa0012D22f Hordeum vulgare seedl... 40 0.14
gb|BM372747.2|BM372747 EBpi05_SQ003_N04_R pistil, 8 DPA, no... 40 0.14
gb|BI778924.2|BI778924 EBro01_SQ002_B06_R root, 3 week, hyd... 40 0.14
gb|BU995095.1|BU995095 HM09D04r HM Hordeum vulgare subsp. v... 40 0.14
gb|BU995190.1|BU995190 HM09H21r HM Hordeum vulgare subsp. v... 40 0.14
gb|BU999179.1|BU999179 HI13I24r HI Hordeum vulgare subsp. v... 40 0.14
gb|CA002377.1|CA002377 HS07E24r HS Hordeum vulgare subsp. v... 40 0.14
gb|CA021844.1|CA021844 HZ41E21r HZ Hordeum vulgare subsp. v... 40 0.14
gb|CV063766.1|CV063766 BNEL92h8 Barley EST endosperm librar... 40 0.14
>gb|BF621527.1|BF621527 HVSMEa0011E23f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011E23f, mRNA sequence
Length = 844
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 232 acccgaagacgtcggagatgaggacgacggcg 201
>gb|BF621761.2|BF621761 HVSMEa0012H13f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012H13f, mRNA sequence
Length = 908
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 172 acccgaagacgtcggagatgaggacgacggcg 141
>gb|BF629242.2|BF629242 HVSMEb0010H15f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0010H15f, mRNA sequence
Length = 518
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 246 acccgaagacgtcggagatgaggacgacggcg 215
>gb|BG300031.1|BG300031 HVSMEb0011B11f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0011B11f, mRNA sequence
Length = 533
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 294 acccgaagacgtcggagatgaggacgacggcg 263
>gb|BF254292.2|BF254292 HVSMEf0003J01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003J01f, mRNA sequence
Length = 883
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 279 acccgaagacgtcggagatgaggacgacggcg 248
>gb|BF258180.2|BF258180 HVSMEf0015A11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015A11f, mRNA sequence
Length = 875
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 243 acccgaagacgtcggagatgaggacgacggcg 212
>gb|BF260055.2|BF260055 HVSMEf0020P20f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020P20f, mRNA sequence
Length = 803
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 281 acccgaagacgtcggagatgaggacgacggcg 250
>gb|BE214264.2|BE214264 HV_CEb0002O24f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0002O24f, mRNA sequence
Length = 982
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 457 acccgaagacgtcggagatgaggacgacggcg 426
>gb|AV932136.1|AV932136 AV932136 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2g20 5', mRNA sequence
Length = 622
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 317 acccgaagacgtcggagatgaggacgacggcg 286
>gb|AV932602.1|AV932602 AV932602 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1f07 5', mRNA sequence
Length = 516
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 284 acccgaagacgtcggagatgaggacgacggcg 253
>gb|AV932651.1|AV932651 AV932651 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1i11 5', mRNA sequence
Length = 589
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 284 acccgaagacgtcggagatgaggacgacggcg 253
>gb|BJ472055.1|BJ472055 BJ472055 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal31o24 5', mRNA sequence
Length = 596
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 291 acccgaagacgtcggagatgaggacgacggcg 260
>gb|BQ467934.1|BQ467934 HR01G10r HR Hordeum vulgare subsp. vulgare cDNA clone HR01G10
5-PRIME, mRNA sequence
Length = 549
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 239 acccgaagacgtcggagatgaggacgacggcg 208
>gb|CA020312.1|CA020312 HV14O23r HV Hordeum vulgare subsp. vulgare cDNA clone HV14O23
5-PRIME, mRNA sequence
Length = 443
Score = 48.1 bits (24), Expect = 6e-004
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 767 acccgaagacgtcggaggcgaggacgacggcg 798
||||||||||||||||| |||||||||||||
Sbjct: 251 acccgaagacgtcggagatgaggacgacggcg 220
>gb|BF630969.1|BF630969 HVSMEb0014J08f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0014J08f, mRNA sequence
Length = 782
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 947 gccgccgccgccgcgagcacggcggcgagg 976
||||||||||||||||| ||| ||||||||
Sbjct: 148 gccgccgccgccgcgaggacgacggcgagg 119
>gb|BI956766.1|BI956766 HVSMEn0005D23f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005D23f, mRNA sequence
Length = 888
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 947 gccgccgccgccgcgagcacggcggcgagg 976
||||||||||||||||| ||| ||||||||
Sbjct: 46 gccgccgccgccgcgaggacgacggcgagg 17
>gb|BI958524.1|BI958524 HVSMEn0015H06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0015H06f, mRNA sequence
Length = 616
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 947 gccgccgccgccgcgagcacggcggcgagg 976
||||||||||||||||| ||| ||||||||
Sbjct: 96 gccgccgccgccgcgaggacgacggcgagg 67
>gb|BF616863.3|BF616863 HVSMEc0013C20f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0013C20f, mRNA sequence
Length = 568
Score = 44.1 bits (22), Expect = 0.009
Identities = 25/26 (96%)
Strand = Plus / Minus
Query: 950 gccgccgccgcgagcacggcggcgag 975
||||||||||||||| ||||||||||
Sbjct: 90 gccgccgccgcgagcgcggcggcgag 65
>gb|BE519613.3|BE519613 HV_CEb0016F21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0016F21f, mRNA sequence
Length = 688
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 947 gccgccgccgccgcgagcacggcggcgagg 976
||||||||||||||||| ||| ||||||||
Sbjct: 139 gccgccgccgccgcgaggacgacggcgagg 110
>gb|BU995967.1|BU995967 HM11O24r HM Hordeum vulgare subsp. vulgare cDNA clone HM11O24
5-PRIME, mRNA sequence
Length = 389
Score = 42.1 bits (21), Expect = 0.037
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 872 cggccagctggcggccgtgga 892
|||||||||||||||||||||
Sbjct: 9 cggccagctggcggccgtgga 29
>gb|BF624166.1|BF624166 HVSMEa0012D22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012D22f, mRNA sequence
Length = 503
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 465 ggtggccgccgccgccgcga 446
>gb|BM372747.2|BM372747 EBpi05_SQ003_N04_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_N04 5', mRNA sequence
Length = 588
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 132 ggtggccgccgccgccgcga 113
>gb|BI778924.2|BI778924 EBro01_SQ002_B06_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_B06 5', mRNA sequence
Length = 551
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 778 tcggaggcgaggacgacggc 797
||||||||||||||||||||
Sbjct: 362 tcggaggcgaggacgacggc 343
>gb|BU995095.1|BU995095 HM09D04r HM Hordeum vulgare subsp. vulgare cDNA clone HM09D04
5-PRIME, mRNA sequence
Length = 222
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 122 ggtggccgccgccgccgcga 103
>gb|BU995190.1|BU995190 HM09H21r HM Hordeum vulgare subsp. vulgare cDNA clone HM09H21
5-PRIME, mRNA sequence
Length = 354
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 121 ggtggccgccgccgccgcga 102
>gb|BU999179.1|BU999179 HI13I24r HI Hordeum vulgare subsp. vulgare cDNA clone HI13I24
5-PRIME, mRNA sequence
Length = 531
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 448 ggtggccgccgccgccgcga 429
>gb|CA002377.1|CA002377 HS07E24r HS Hordeum vulgare subsp. vulgare cDNA clone HS07E24
5-PRIME, mRNA sequence
Length = 376
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 943 ggtggccgccgccgccgcga 962
||||||||||||||||||||
Sbjct: 373 ggtggccgccgccgccgcga 354
>gb|CA021844.1|CA021844 HZ41E21r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ41E21
5-PRIME, mRNA sequence
Length = 361
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 778 tcggaggcgaggacgacggc 797
||||||||||||||||||||
Sbjct: 252 tcggaggcgaggacgacggc 233
>gb|CV063766.1|CV063766 BNEL92h8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL92h8 5' similar to zinc finger,
mRNA sequence
Length = 574
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 944 gtggccgccgccgccgcgag 963
||||||||||||||||||||
Sbjct: 540 gtggccgccgccgccgcgag 559
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 200,494
Number of Sequences: 312970
Number of extensions: 200494
Number of successful extensions: 78791
Number of sequences better than 0.5: 29
Number of HSP's better than 0.5 without gapping: 29
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 78738
Number of HSP's gapped (non-prelim): 53
length of query: 1053
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1034
effective length of database: 169,188,109
effective search space: 174940504706
effective search space used: 174940504706
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)