BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716365.2.1
(936 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. v... 416 e-115
gb|BF254638.2|BF254638 HVSMEf0004J12f Hordeum vulgare seedl... 349 1e-094
gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. v... 335 2e-090
gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. v... 248 3e-064
gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa... 230 7e-059
gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. v... 86 2e-015
gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subs... 50 1e-004
>gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. vulgare cDNA clone HI01I09
3-PRIME, mRNA sequence
Length = 677
Score = 416 bits (210), Expect = e-115
Identities = 333/374 (89%)
Strand = Plus / Plus
Query: 333 cgagggatctcaactggcttggggatatcaggtggagtggcacacctgattaatgcccaa 392
|||||||| |||||||||||||||||||| || ||||| |||||||| || ||||||||
Sbjct: 302 cgagggatttcaactggcttggggatatctggaggagtagcacaccttatcaatgcccag 361
Query: 393 ttaacaccttcaaagaacgggtgctgttttatttctgtggctccacgcttatacgctaat 452
|||||||||||||||||||||||||| ||||||||||| ||||| ||||| || || |||
Sbjct: 362 ttaacaccttcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaat 421
Query: 453 cgatgctgaggttccttgatgagcaatcccctaataagatcccttgcagcaaaactcact 512
|||||||| || |||||||| ||||| ||||| || ||||||||||||||||||| |||
Sbjct: 422 cgatgctgtggctccttgatcagcaacccccttatgagatcccttgcagcaaaacccaca 481
Query: 513 acaggtgattctgggaaccttaagggttgtccaactacgttgaacaatgtcgccctattc 572
|||||||||||||||||||| | ||| || ||||| || ||||| | || ||||| |||
Sbjct: 482 acaggtgattctgggaacctgaggggctggccaacaacattgaatagcgttgccctgttc 541
Query: 573 ccagaacctttgaatggtgtcttgccgaagagaagctcatacaagaatatgccaaatgtc 632
|| |||||||||||||| ||||||||||| ||||| |||||||||||||||||||| |||
Sbjct: 542 cccgaacctttgaatggagtcttgccgaaaagaagttcatacaagaatatgccaaaagtc 601
Query: 633 caccagtctacagcacttccatgcccctcgccctttataatttcaggtgccaagtactca 692
||||||||||||||||| |||||||||||||||||||| || |||||||||||||| |||
Sbjct: 602 caccagtctacagcactcccatgcccctcgccctttatgatctcaggtgccaagtattca 661
Query: 693 tgggtgccaacaaa 706
||||||||||||||
Sbjct: 662 tgggtgccaacaaa 675
>gb|BF254638.2|BF254638 HVSMEf0004J12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0004J12f, mRNA sequence
Length = 816
Score = 349 bits (176), Expect = 1e-094
Identities = 316/362 (87%), Gaps = 3/362 (0%)
Strand = Plus / Minus
Query: 564 gccctattcccagaacctttgaatggtgtcttgccgaagagaagctcatacaagaatatg 623
||||| ||||| ||||||||||||| |||| ||| || | ||| |||| ||||||||||
Sbjct: 362 gccctgttccccgaacctttgaatgcagtctcgcccaaaacaagttcatccaagaatatg 303
Query: 624 ccaaatgtccaccagtctacagcacttccatgcccctcgccctttataatttcaggtgcc 683
|| || |||||||||| ||||||||| |||||||||||||||||||| || |||||||||
Sbjct: 302 cctaaagtccaccagtttacagcactcccatgcccctcgccctttatgatctcaggtgcc 243
Query: 684 aagtactcatgggtgccaacaaaagacatagaccttgcatctgtcggctctgcaacaagc 743
||||| ||||||||||||||||| |||||||||||||||||||| |||||||||||||||
Sbjct: 242 aagtattcatgggtgccaacaaacgacatagaccttgcatctgtgggctctgcaacaagc 183
Query: 744 tcaggaagtggtctgacctgatttgccaagtctgcctttgcctttttatcttttttctc- 802
||||| |||||||||||||| |||||| ||||||||||||||| || || || |||||
Sbjct: 182 tcagggagtggtctgacctgggttgccatgtctgcctttgccttcttttccttcttctcc 123
Query: 803 --cttggatttggaagagaaaaagcgaggagagaagcatgtggttgtcgtgacacaagaa 860
||| || ||||| |||||||||||||||||||||||||| |||||||| ||||||| |
Sbjct: 122 ctcttagacttggaggagaaaaagcgaggagagaagcatgtagttgtcgtaacacaagca 63
Query: 861 ggctgcatacaggcaggctcaatgcacacaggctgcacacagtaagctgggttgcccttt 920
||||| |||||||| ||||| |||||||| || || ||||| |||||| || | ||||||
Sbjct: 62 ggctggatacaggctggctcgatgcacacgggttgtacacaataagcttgggttcccttt 3
Query: 921 tg 922
||
Sbjct: 2 tg 1
>gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
5-PRIME, mRNA sequence
Length = 617
Score = 335 bits (169), Expect = 2e-090
Identities = 280/317 (88%)
Strand = Plus / Minus
Query: 333 cgagggatctcaactggcttggggatatcaggtggagtggcacacctgattaatgcccaa 392
|||||||| |||||||||||||||||||| || ||||| |||||||| || ||||||||
Sbjct: 355 cgagggatttcaactggcttggggatatctggaggagtagcacaccttatcaatgcccag 296
Query: 393 ttaacaccttcaaagaacgggtgctgttttatttctgtggctccacgcttatacgctaat 452
|||||||||||||||||||||||||| ||||||||||| ||||| ||||| || || |||
Sbjct: 295 ttaacaccttcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaat 236
Query: 453 cgatgctgaggttccttgatgagcaatcccctaataagatcccttgcagcaaaactcact 512
|||||||| || |||||||| ||||| ||||| || ||||||||||||||||||| |||
Sbjct: 235 cgatgctgtggctccttgatcagcaacccccttatgagatcccttgcagcaaaacccaca 176
Query: 513 acaggtgattctgggaaccttaagggttgtccaactacgttgaacaatgtcgccctattc 572
|||||||||||||||||||| | ||| || ||||| || ||||| | || ||||| |||
Sbjct: 175 acaggtgattctgggaacctgaggggctggccaacaacattgaatagcgttgccctgttc 116
Query: 573 ccagaacctttgaatggtgtcttgccgaagagaagctcatacaagaatatgccaaatgtc 632
|| |||||||||||||| ||||||||||| ||||| |||||||||||||||||||| |||
Sbjct: 115 cccgaacctttgaatggagtcttgccgaaaagaagttcatacaagaatatgccaaaagtc 56
Query: 633 caccagtctacagcact 649
|||||||||||||||||
Sbjct: 55 caccagtctacagcact 39
>gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
3-PRIME, mRNA sequence
Length = 589
Score = 248 bits (125), Expect = 3e-064
Identities = 224/257 (87%)
Strand = Plus / Plus
Query: 333 cgagggatctcaactggcttggggatatcaggtggagtggcacacctgattaatgcccaa 392
|||||||| |||||||||||||||||||| || ||||| |||||||| || ||||||||
Sbjct: 329 cgagggatttcaactggcttggggatatctggaggagtagcacaccttatcaatgcccag 388
Query: 393 ttaacaccttcaaagaacgggtgctgttttatttctgtggctccacgcttatacgctaat 452
|||||||||||||||||||||||||| ||||||||||| ||||| ||||| || || |||
Sbjct: 389 ttaacaccttcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaat 448
Query: 453 cgatgctgaggttccttgatgagcaatcccctaataagatcccttgcagcaaaactcact 512
|||||||| || |||||||| ||||| ||||| || ||||||||||||||||||| |||
Sbjct: 449 cgatgctgtggctccttgatcagcaacccccttatgagatcccttgcagcaaaacccaca 508
Query: 513 acaggtgattctgggaaccttaagggttgtccaactacgttgaacaatgtcgccctattc 572
|||||||||||||||||||| | ||| || ||||| || ||||| | || ||||| |||
Sbjct: 509 acaggtgattctgggaacctgaggggctggccaacaacattgaatagcgttgccctgttc 568
Query: 573 ccagaacctttgaatgg 589
|| ||||||||||||||
Sbjct: 569 cccgaacctttgaatgg 585
>gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0004P24f, mRNA sequence
Length = 632
Score = 230 bits (116), Expect = 7e-059
Identities = 179/200 (89%)
Strand = Plus / Minus
Query: 333 cgagggatctcaactggcttggggatatcaggtggagtggcacacctgattaatgcccaa 392
|||||||| |||||||||||||||||||| || ||||| |||||||| || ||||||||
Sbjct: 247 cgagggatttcaactggcttggggatatctggaggagtagcacaccttatcaatgcccag 188
Query: 393 ttaacaccttcaaagaacgggtgctgttttatttctgtggctccacgcttatacgctaat 452
|||||||||||||||||||||||||| ||||||||||| ||||| ||||| || || |||
Sbjct: 187 ttaacaccttcaaagaacgggtgctgctttatttctgtcgctccgcgcttgtaggccaat 128
Query: 453 cgatgctgaggttccttgatgagcaatcccctaataagatcccttgcagcaaaactcact 512
|||||||| || |||||||| ||||| ||||| || ||||||||||||||||||| |||
Sbjct: 127 cgatgctgtggctccttgatcagcaacccccttatgagatcccttgcagcaaaacccaca 68
Query: 513 acaggtgattctgggaacct 532
||||||||||||||||||||
Sbjct: 67 acaggtgattctgggaacct 48
>gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. vulgare cDNA clone HX06B10
3-PRIME, mRNA sequence
Length = 585
Score = 85.7 bits (43), Expect = 2e-015
Identities = 66/74 (89%)
Strand = Plus / Plus
Query: 576 gaacctttgaatggtgtcttgccgaagagaagctcatacaagaatatgccaaatgtccac 635
||||||||||| ||||| |||||||| |||||||| || |||||||| ||||| ||||||
Sbjct: 511 gaacctttgaaaggtgtnttgccgaacagaagctcgtataagaatataccaaaggtccac 570
Query: 636 cagtctacagcact 649
||||| ||||||||
Sbjct: 571 cagtcaacagcact 584
Score = 50.1 bits (25), Expect = 1e-004
Identities = 49/57 (85%)
Strand = Plus / Plus
Query: 387 gcccaattaacaccttcaaagaacgggtgctgttttatttctgtggctccacgctta 443
|||||||| ||||| ||||| || || ||||||||||| || ||||| |||||||||
Sbjct: 322 gcccaattcacaccctcaaaaaatggatgctgttttatctcagtggcaccacgctta 378
>gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200081A03F1, mRNA sequence
Length = 360
Score = 50.1 bits (25), Expect = 1e-004
Identities = 49/57 (85%)
Strand = Plus / Minus
Query: 387 gcccaattaacaccttcaaagaacgggtgctgttttatttctgtggctccacgctta 443
|||||||| ||||| ||||| || || ||||||||||| || ||||| |||||||||
Sbjct: 179 gcccaattcacaccctcaaaaaatggatgctgttttatctcagtggcaccacgctta 123
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 77,242
Number of Sequences: 312970
Number of extensions: 77242
Number of successful extensions: 18905
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18884
Number of HSP's gapped (non-prelim): 20
length of query: 936
length of database: 175,134,539
effective HSP length: 19
effective length of query: 917
effective length of database: 169,188,109
effective search space: 155145495953
effective search space used: 155145495953
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)