BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131572.2.6
(1100 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL450686.1|AL450686 AL450686 Hordeum vulgare Barke etiol... 46 0.002
gb|AL504052.1|AL504052 AL504052 Hordeum vulgare Barke roots... 46 0.002
gb|BF258733.1|BF258733 HVSMEf0016I14f Hordeum vulgare seedl... 46 0.002
gb|AV918077.1|AV918077 AV918077 K. Sato unpublished cDNA li... 46 0.002
gb|AV918703.1|AV918703 AV918703 K. Sato unpublished cDNA li... 46 0.002
gb|AV918926.1|AV918926 AV918926 K. Sato unpublished cDNA li... 46 0.002
gb|AV921351.1|AV921351 AV921351 K. Sato unpublished cDNA li... 46 0.002
gb|BM817346.1|BM817346 HC111F01_T3.ab1 HC Hordeum vulgare s... 46 0.002
gb|BJ468058.1|BJ468058 BJ468058 K. Sato unpublished cDNA li... 46 0.002
gb|BJ468335.1|BJ468335 BJ468335 K. Sato unpublished cDNA li... 46 0.002
gb|CD663222.1|CD663222 UCRHV18_04db05_b1 Drought-stressed D... 46 0.002
gb|CV060228.1|CV060228 BNEL55g8 Barley EST endosperm librar... 46 0.002
gb|BF257975.2|BF257975 HVSMEf0014G07f Hordeum vulgare seedl... 44 0.010
gb|BI947519.1|BI947519 HVSMEl0005M02f Hordeum vulgare spike... 44 0.010
gb|AL501392.1|AL501392 AL501392 Hordeum vulgare Barke roots... 40 0.15
gb|AL504415.1|AL504415 AL504415 Hordeum vulgare Barke roots... 40 0.15
gb|BF257368.2|BF257368 HVSMEf0012L06f Hordeum vulgare seedl... 40 0.15
gb|BF259978.2|BF259978 HVSMEf0020M11f Hordeum vulgare seedl... 40 0.15
gb|BF260951.2|BF260951 HVSMEf0023F23f Hordeum vulgare seedl... 40 0.15
gb|BG309181.2|BG309181 HVSMEc0004K06f Hordeum vulgare seedl... 40 0.15
gb|BF259024.3|BF259024 HVSMEf0017H01f Hordeum vulgare seedl... 40 0.15
gb|CB882435.1|CB882435 HL01N17w HL Hordeum vulgare subsp. v... 40 0.15
>gb|AL450686.1|AL450686 AL450686 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03M11u 5', mRNA sequence
Length = 540
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 293 ttcaccaaagcagggttcagcga 271
>gb|AL504052.1|AL504052 AL504052 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04A17V 5', mRNA sequence
Length = 661
Score = 46.1 bits (23), Expect = 0.002
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 110 gggctggccgagtggttcaggaaggac 136
||||||||||| |||||||||||||||
Sbjct: 428 gggctggccgactggttcaggaaggac 454
>gb|BF258733.1|BF258733 HVSMEf0016I14f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0016I14f, mRNA sequence
Length = 964
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 498 tgctaaagcttgtgctgcaccac 520
|||||||||||||||||||||||
Sbjct: 522 tgctaaagcttgtgctgcaccac 544
>gb|AV918077.1|AV918077 AV918077 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17l08 3', mRNA sequence
Length = 640
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 283 ttcaccaaagcagggttcagcga 261
>gb|AV918703.1|AV918703 AV918703 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21p04 3', mRNA sequence
Length = 654
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 284 ttcaccaaagcagggttcagcga 262
>gb|AV918926.1|AV918926 AV918926 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags22l17 3', mRNA sequence
Length = 655
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 286 ttcaccaaagcagggttcagcga 264
>gb|AV921351.1|AV921351 AV921351 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23c06 3', mRNA sequence
Length = 657
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 284 ttcaccaaagcagggttcagcga 262
>gb|BM817346.1|BM817346 HC111F01_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC111F01_T3.ab1 similar to Hexaprenyldihydroxybenzoate
methyltransferase,Apigenin
4'-O-methyltransferase,[Myelin basic protein]-arginine
N-methyltransferase,Catechol
O-methyltransferase,Glycoprotein-fucosylgalactoside
alpha-galactosyltransferase,, mRNA sequence
Length = 905
Score = 46.1 bits (23), Expect = 0.002
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 110 gggctggccgagtggttcaggaaggac 136
||||||||||| |||||||||||||||
Sbjct: 65 gggctggccgactggttcaggaaggac 91
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Plus
Query: 717 agctcttcaccaaagcagggttcagcgaatacaagattctcaag 760
||||||||| |||||||||||||| || || |||||| |||||
Sbjct: 679 agctcttcatgaaagcagggttcagtgactataagattgtcaag 722
>gb|BJ468058.1|BJ468058 BJ468058 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30o16 3', mRNA sequence
Length = 674
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 304 ttcaccaaagcagggttcagcga 282
>gb|BJ468335.1|BJ468335 BJ468335 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38e18 3', mRNA sequence
Length = 744
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 283 ttcaccaaagcagggttcagcga 261
>gb|CD663222.1|CD663222 UCRHV18_04db05_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_04db05, mRNA sequence
Length = 545
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 722 ttcaccaaagcagggttcagcga 744
|||||||||||||||||||||||
Sbjct: 301 ttcaccaaagcagggttcagcga 279
>gb|CV060228.1|CV060228 BNEL55g8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL55g8 5' similar to putative
o-methyltransferase, mRNA sequence
Length = 635
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 359 attgccaaggccttcccacacat 381
|||||||||||||||||||||||
Sbjct: 398 attgccaaggccttcccacacat 420
>gb|BF257975.2|BF257975 HVSMEf0014G07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014G07f, mRNA sequence
Length = 569
Score = 44.1 bits (22), Expect = 0.010
Identities = 40/46 (86%)
Strand = Plus / Plus
Query: 91 ctacatcgagccggcaatagggctggccgagtggttcaggaaggac 136
|||||| ||| |||| || || |||||||| |||||||||||||||
Sbjct: 220 ctacatggaggcggccatgggcctggccgactggttcaggaaggac 265
>gb|BI947519.1|BI947519 HVSMEl0005M02f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0005M02f, mRNA sequence
Length = 983
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 283 cagggacttgttcgaggggatc 304
||||||||||||||||||||||
Sbjct: 578 cagggacttgttcgaggggatc 599
>gb|AL501392.1|AL501392 AL501392 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04A17u 3', mRNA sequence
Length = 539
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 717 agctcttcaccaaagcagggttcagcgaatacaagattctcaag 760
||||||||| |||||||||||||| || || |||||| |||||
Sbjct: 221 agctcttcatgaaagcagggttcagtgactataagattgtcaag 178
>gb|AL504415.1|AL504415 AL504415 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05D13V 5', mRNA sequence
Length = 684
Score = 40.1 bits (20), Expect = 0.15
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 113 ctggccgagtggttcaggaaggac 136
|||||||| |||||||||||||||
Sbjct: 55 ctggccgactggttcaggaaggac 78
>gb|BF257368.2|BF257368 HVSMEf0012L06f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012L06f, mRNA sequence
Length = 539
Score = 40.1 bits (20), Expect = 0.15
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 113 ctggccgagtggttcaggaaggac 136
|||||||| |||||||||||||||
Sbjct: 456 ctggccgactggttcaggaaggac 479
>gb|BF259978.2|BF259978 HVSMEf0020M11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M11f, mRNA sequence
Length = 541
Score = 40.1 bits (20), Expect = 0.15
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 113 ctggccgagtggttcaggaaggac 136
|||||||| |||||||||||||||
Sbjct: 256 ctggccgactggttcaggaaggac 279
>gb|BF260951.2|BF260951 HVSMEf0023F23f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023F23f, mRNA sequence
Length = 824
Score = 40.1 bits (20), Expect = 0.15
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 365 aaggccttcccacacataaaatgcactgtgct 396
||||||||||| |||||||| ||||| |||||
Sbjct: 710 aaggccttcccgcacataaactgcaccgtgct 741
>gb|BG309181.2|BG309181 HVSMEc0004K06f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0004K06f, mRNA sequence
Length = 826
Score = 40.1 bits (20), Expect = 0.15
Identities = 45/52 (86%), Gaps = 1/52 (1%)
Strand = Plus / Plus
Query: 353 agggccattg-ccaaggccttcccacacataaaatgcactgtgctggcccct 403
|||||||||| ||||||||||||| || ||||| |||| |||| ||| ||||
Sbjct: 641 agggccattgtccaaggccttcccgcatataaagtgcaatgtgttggaccct 692
>gb|BF259024.3|BF259024 HVSMEf0017H01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0017H01f, mRNA sequence
Length = 804
Score = 40.1 bits (20), Expect = 0.15
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 113 ctggccgagtggttcaggaaggac 136
|||||||| |||||||||||||||
Sbjct: 457 ctggccgactggttcaggaaggac 480
>gb|CB882435.1|CB882435 HL01N17w HL Hordeum vulgare subsp. vulgare cDNA clone HL01N17
3-PRIME, mRNA sequence
Length = 553
Score = 40.1 bits (20), Expect = 0.15
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 113 ctggccgagtggttcaggaaggac 136
|||||||| |||||||||||||||
Sbjct: 461 ctggccgactggttcaggaaggac 484
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 99,364
Number of Sequences: 312970
Number of extensions: 99364
Number of successful extensions: 26495
Number of sequences better than 0.5: 22
Number of HSP's better than 0.5 without gapping: 21
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 26460
Number of HSP's gapped (non-prelim): 36
length of query: 1100
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1081
effective length of database: 169,188,109
effective search space: 182892345829
effective search space used: 182892345829
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)