BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QBE1h10.xg.2.1
         (463 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN816688.1|CN816688  HRO4523_F05_L09ZS5 Lib AA071E1X Aven...   100   2e-021
gb|CN816536.1|CN816536  HRO4521_H04_O07ZS5 Lib AA071E1X Aven...    32   0.41 

>gb|CN816688.1|CN816688 HRO4523_F05_L09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4523_F05_L09, mRNA sequence
          Length = 632

 Score = 99.6 bits (50), Expect = 2e-021
 Identities = 135/166 (81%)
 Strand = Plus / Plus

                                                                       
Query: 212 gagagcttcggcgcgggaggcaagacgtgcatcctgtcacgggtgtacccgtccatcgcc 271
           |||||||||||||||||||| || || |||||||||||| |||||||||||||||| || 
Sbjct: 22  gagagcttcggcgcgggagggaaaacctgcatcctgtcaagggtgtacccgtccatggcg 81

                                                                       
Query: 272 gtcgggaaggacgctcacctctacgttttcaacaacggcgagnnngacgtcacnnnntcc 331
            | ||||| ||||| |||||  |||| ||||||||||| | |   ||  |||     |||
Sbjct: 82  ttagggaaagacgcgcaccttcacgtgttcaacaacggggcgacggatatcaaggtgtcc 141

                                                         
Query: 332 ggtctgaccgcctgggagatgaagaaaccnctgatgaacggcgcct 377
            | |||||||| |||||||||||||||||  ||||||| |||||||
Sbjct: 142 cgcctgaccgcgtgggagatgaagaaaccattgatgaatggcgcct 187



>gb|CN816536.1|CN816536 HRO4521_H04_O07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4521_H04_O07, mRNA sequence
          Length = 862

 Score = 32.2 bits (16), Expect = 0.41
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 187 aagcttgatcgatcgg 202
           ||||||||||||||||
Sbjct: 643 aagcttgatcgatcgg 628



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 709
Number of Sequences: 8143
Number of extensions: 709
Number of successful extensions: 267
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 264
Number of HSP's gapped (non-prelim): 3
length of query: 463
length of database: 4,702,463
effective HSP length: 16
effective length of query: 447
effective length of database: 4,572,175
effective search space: 2043762225
effective search space used: 2043762225
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)