BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF12e06.yg.2.1
(561 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 52 5e-007
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 52 5e-007
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 52.0 bits (26), Expect = 5e-007
Identities = 59/70 (84%)
Strand = Plus / Plus
Query: 471 ctgcactacaatggtgtgatgaaaccttggcttgacttggggatacttgattacaagaac 530
|||||||||||||| |||||||| |||||||| || || ||||| || ||||||||
Sbjct: 430 ctgcactacaatggcaatatgaaaccgtggcttgagttaggcatactggaatacaagaag 489
Query: 531 tactggagga 540
||||||||||
Sbjct: 490 tactggagga 499
Score = 34.2 bits (17), Expect = 0.13
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 420 cttggacatgattatggaatc 440
|||||| ||||||||||||||
Sbjct: 379 cttggatatgattatggaatc 399
Score = 34.2 bits (17), Expect = 0.13
Identities = 26/29 (89%)
Strand = Plus / Plus
Query: 241 tgaatgtcattgaattggataaatggagg 269
||||||| ||| | |||||||||||||||
Sbjct: 200 tgaatgtgattaatttggataaatggagg 228
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 52.0 bits (26), Expect = 5e-007
Identities = 59/70 (84%)
Strand = Plus / Plus
Query: 471 ctgcactacaatggtgtgatgaaaccttggcttgacttggggatacttgattacaagaac 530
|||||||||||||| |||||||||||||| || || || ||||| || ||||||||
Sbjct: 493 ctgcactacaatggcaatatgaaaccttggctcgagttaggcatactggaatacaagaag 552
Query: 531 tactggagga 540
||||||||||
Sbjct: 553 tactggagga 562
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1190
Number of Sequences: 8143
Number of extensions: 1190
Number of successful extensions: 307
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 301
Number of HSP's gapped (non-prelim): 5
length of query: 561
length of database: 4,702,463
effective HSP length: 16
effective length of query: 545
effective length of database: 4,572,175
effective search space: 2491835375
effective search space used: 2491835375
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)