BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923331.2.1
(496 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Aven... 103 1e-022
gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Aven... 66 3e-011
gb|CN815981.1|CN815981 HRO4515_E12_J23ZS5 Lib AA071E1X Aven... 38 0.007
>gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4472_D08_G16, mRNA sequence
Length = 842
Score = 103 bits (52), Expect = 1e-022
Identities = 172/212 (81%)
Strand = Plus / Minus
Query: 191 gtagttacacaaaaattacttcaagctttgcacagggatggtctttgatatattgctcga 250
||||||| ||||||||||| |||||||||||||| ||||| ||||| || ||| | | |
Sbjct: 664 gtagttatacaaaaattacctcaagctttgcacatggatgatctttaatgtatcgagcaa 605
Query: 251 gttcagaatcatccctaaatctaaaaccgtacaaagcctgacgttgcgttagaccgactg 310
|||||| |||||| |||||| |||| ||| || || | |||| || ||||| ||||
Sbjct: 604 gttcagcatcatcactaaatttaaatttgtataatgcttctcgttctgtaagaccaactg 545
Query: 311 ggtaaattctgtataatttcaggtcatccccacctctaatgctcgggggcctttcatcct 370
| ||||||||||| | |||| ||||||||||||| ||||| | |||||||||||||
Sbjct: 544 gataaattctgtacaccttcaactcatccccacctcgtatgcttgatggcctttcatcct 485
Query: 371 tccatacaggaacgtggttgagggacctaaat 402
|||||| |||||| ||||||| ||| ||||||
Sbjct: 484 tccatataggaacatggttgatggatctaaat 453
Score = 46.1 bits (23), Expect = 3e-005
Identities = 32/35 (91%)
Strand = Plus / Minus
Query: 457 taaatgctctctgacttaaaccgccctctctctct 491
|||||||| |||| |||||||| ||||||||||||
Sbjct: 398 taaatgctttctgtcttaaacctccctctctctct 364
>gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4458_D09_H18, mRNA sequence
Length = 542
Score = 65.9 bits (33), Expect = 3e-011
Identities = 42/45 (93%)
Strand = Plus / Minus
Query: 358 ggcctttcatccttccatacaggaacgtggttgagggacctaaat 402
|||||||||||||||||||||||||| ||||||| ||| ||||||
Sbjct: 325 ggcctttcatccttccatacaggaacatggttgatggatctaaat 281
Score = 44.1 bits (22), Expect = 1e-004
Identities = 41/46 (89%), Gaps = 1/46 (2%)
Strand = Plus / Minus
Query: 192 tagttacacaaaaatt-acttcaagctttgcacagggatggtcttt 236
|||||| ||||||||| || |||||||||||||| ||||| |||||
Sbjct: 490 tagttatacaaaaattnacctcaagctttgcacatggatgatcttt 445
Score = 38.2 bits (19), Expect = 0.007
Identities = 31/35 (88%)
Strand = Plus / Minus
Query: 457 taaatgctctctgacttaaaccgccctctctctct 491
|||||||| |||| ||||| || ||||||||||||
Sbjct: 226 taaatgctttctgtcttaagcctccctctctctct 192
>gb|CN815981.1|CN815981 HRO4515_E12_J23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_E12_J23, mRNA sequence
Length = 639
Score = 38.2 bits (19), Expect = 0.007
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 25 gaaaagaaggaatgatgtt 43
|||||||||||||||||||
Sbjct: 120 gaaaagaaggaatgatgtt 138
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1477
Number of Sequences: 8143
Number of extensions: 1477
Number of successful extensions: 300
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 291
Number of HSP's gapped (non-prelim): 9
length of query: 496
length of database: 4,702,463
effective HSP length: 16
effective length of query: 480
effective length of database: 4,572,175
effective search space: 2194644000
effective search space used: 2194644000
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)