BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3067450.2.1
         (724 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN819167.1|CN819167  HRO4468_E11_I22ZS5 Lib AA070E1X Aven...    38   0.011
gb|CN814875.1|CN814875  HRO4503_D10_H19ZS5 Lib AA071E1X Aven...    36   0.042
gb|CN816486.1|CN816486  HRO4521_C09_E17ZS5 Lib AA071E1X Aven...    34   0.16 
gb|CN817629.1|CN817629  HRO4463_B12_C23ZS5 Lib AA070E1X Aven...    34   0.16 

>gb|CN819167.1|CN819167 HRO4468_E11_I22ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4468_E11_I22, mRNA sequence
          Length = 852

 Score = 38.2 bits (19), Expect = 0.011
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 305 ataaaaaagagatcagaca 323
           |||||||||||||||||||
Sbjct: 811 ataaaaaagagatcagaca 829



>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4503_D10_H19, mRNA sequence
          Length = 846

 Score = 36.2 bits (18), Expect = 0.042
 Identities = 69/86 (80%)
 Strand = Plus / Minus

                                                                       
Query: 365 ccaaccacggtatctactattatcaccttgccgcctgcatctcgtggaggtatagctttc 424
           ||||||||  |||| || ||||||||||| || || |||||||  | ||| ||||||| |
Sbjct: 107 ccaaccaccatatcaacaattatcaccttcccaccggcatctctggaagggatagcttcc 48

                                     
Query: 425 ttgcagttctttagaatcgtgacaca 450
           ||||| || || || ||| |||||||
Sbjct: 47  ttgcattttttcagtatcttgacaca 22



>gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4521_C09_E17, mRNA sequence
          Length = 431

 Score = 34.2 bits (17), Expect = 0.16
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 136 acatgtaggtgatgagg 152
           |||||||||||||||||
Sbjct: 208 acatgtaggtgatgagg 224



>gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4463_B12_C23, mRNA sequence
          Length = 781

 Score = 34.2 bits (17), Expect = 0.16
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 136 acatgtaggtgatgagg 152
           |||||||||||||||||
Sbjct: 508 acatgtaggtgatgagg 524



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1474
Number of Sequences: 8143
Number of extensions: 1474
Number of successful extensions: 457
Number of sequences better than  0.5: 4
Number of HSP's better than  0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 452
Number of HSP's gapped (non-prelim): 5
length of query: 724
length of database: 4,702,463
effective HSP length: 16
effective length of query: 708
effective length of database: 4,572,175
effective search space: 3237099900
effective search space used: 3237099900
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)