BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455878.2.1
(1327 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 64 3e-010
gb|CN816150.1|CN816150 HRO4517_E08_I15ZS5 Lib AA071E1X Aven... 54 3e-007
gb|CN814894.1|CN814894 HRO4503_F08_L15ZS5 Lib AA071E1X Aven... 52 1e-006
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 50 5e-006
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 50 5e-006
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 42 0.001
gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Aven... 40 0.005
gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Aven... 40 0.005
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 40 0.005
gb|CN815015.1|CN815015 HRO4504_H10_P20ZS5 Lib AA071E1X Aven... 36 0.077
gb|CN817527.1|CN817527 HRO4464_A04_A08ZS5 Lib AA070E1X Aven... 36 0.077
gb|CN820179.1|CN820179 HRO4412_A09_B18ZS5 Lib AA069E1X Aven... 36 0.077
gb|CN814931.1|CN814931 HRO4504_A09_B18ZS5 Lib AA071E1X Aven... 34 0.31
gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Aven... 34 0.31
gb|CN819744.1|CN819744 HRO4403_F04_L07ZS5 Lib AA069E1X Aven... 34 0.31
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 63.9 bits (32), Expect = 3e-010
Identities = 95/116 (81%)
Strand = Plus / Plus
Query: 791 gctgtcatgctcaagctcgtcctgcacttctggagcgacgacgactgcgtcaagatcctg 850
||||| ||||||||||| || || ||||| |||||||| || || || || || |||||
Sbjct: 138 gctgtgatgctcaagcttgtgcttcacttttggagcgatgaggattgtgtgaaaatccta 197
Query: 851 gagctgtgcaggaaggccatcccttcccgcgaagaaggagggaaggtgatcatcat 906
| | ||||||||||||||| ||||| ||||| || ||||||||||| || |||||
Sbjct: 198 gcccagtgcaggaaggccattccttcacgcgaggagggagggaaggtaataatcat 253
Score = 36.2 bits (18), Expect = 0.077
Identities = 21/22 (95%)
Strand = Plus / Plus
Query: 1085 gtcatcgaggtctacccgtaag 1106
||||||||||| ||||||||||
Sbjct: 432 gtcatcgaggtttacccgtaag 453
>gb|CN816150.1|CN816150 HRO4517_E08_I15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E08_I15, mRNA sequence
Length = 455
Score = 54.0 bits (27), Expect = 3e-007
Identities = 51/59 (86%)
Strand = Plus / Plus
Query: 1048 cgactacaaggttgtcaagaaaatcggagctcgtggtgtcatcgaggtctacccgtaag 1106
|||||| ||| || ||||||| | |||||||| |||||||||||||||||||| ||||
Sbjct: 141 cgactataagattatcaagaagctgggagctcggggtgtcatcgaggtctacccataag 199
Score = 36.2 bits (18), Expect = 0.077
Identities = 24/26 (92%)
Strand = Plus / Plus
Query: 951 cccagctgctgatggacatgctcatg 976
|||||||||||||||| ||||||||
Sbjct: 44 cccagctgctgatggatgtgctcatg 69
>gb|CN814894.1|CN814894 HRO4503_F08_L15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_F08_L15, mRNA sequence
Length = 567
Score = 52.0 bits (26), Expect = 1e-006
Identities = 32/34 (94%)
Strand = Plus / Plus
Query: 1073 ggagctcgtggtgtcatcgaggtctacccgtaag 1106
|||||||| |||||||||||||||||||| ||||
Sbjct: 180 ggagctcggggtgtcatcgaggtctacccataag 213
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 50.1 bits (25), Expect = 5e-006
Identities = 88/109 (80%)
Strand = Plus / Plus
Query: 798 tgctcaagctcgtcctgcacttctggagcgacgacgactgcgtcaagatcctggagctgt 857
|||||||||| || |||||| |||||| || |||||||| || |||||| || | ||
Sbjct: 179 tgctcaagcttgtgctgcaccactggagtgatgacgactgtgtgaagatcttgtctcagt 238
Query: 858 gcaggaaggccatcccttcccgcgaagaaggagggaaggtgatcatcat 906
| |||| ||||| ||||| || || || ||||||||||||||||||||
Sbjct: 239 gtaggagtgccattccttcacgtgaggagggagggaaggtgatcatcat 287
Score = 38.2 bits (19), Expect = 0.020
Identities = 43/51 (84%)
Strand = Plus / Plus
Query: 1049 gactacaaggttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||| || |||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 430 gactataaagttgtcaagaaattaggagcacgaggtgtcttcgaagtctac 480
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 50.1 bits (25), Expect = 5e-006
Identities = 88/109 (80%)
Strand = Plus / Plus
Query: 798 tgctcaagctcgtcctgcacttctggagcgacgacgactgcgtcaagatcctggagctgt 857
|||||||||| || |||||| |||||| || |||||||| || |||||| || | ||
Sbjct: 126 tgctcaagcttgtgctgcaccactggagtgatgacgactgtgtgaagatcttgtctcagt 185
Query: 858 gcaggaaggccatcccttcccgcgaagaaggagggaaggtgatcatcat 906
| |||| ||||| ||||| || || || ||||||||||||||||||||
Sbjct: 186 gtaggagtgccattccttcacgtgaggagggagggaaggtgatcatcat 234
Score = 38.2 bits (19), Expect = 0.020
Identities = 43/51 (84%)
Strand = Plus / Plus
Query: 1049 gactacaaggttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||| || |||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 377 gactataaagttgtcaagaaattaggagcacgaggtgtcttcgaagtctac 427
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 42.1 bits (21), Expect = 0.001
Identities = 36/41 (87%)
Strand = Plus / Plus
Query: 866 gccatcccttcccgcgaagaaggagggaaggtgatcatcat 906
||||| ||||| || || || ||||||||||||||||||||
Sbjct: 140 gccattccttcacgtgaggagggagggaaggtgatcatcat 180
Score = 34.2 bits (17), Expect = 0.31
Identities = 35/41 (85%)
Strand = Plus / Plus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 333 ttgtcaagaaagtaggagcacgaggtgtcttcgaagtctac 373
>gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_E05_I10, mRNA sequence
Length = 596
Score = 40.1 bits (20), Expect = 0.005
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 887 ggagggaaggtgatcatcat 906
||||||||||||||||||||
Sbjct: 477 ggagggaaggtgatcatcat 458
Score = 34.2 bits (17), Expect = 0.31
Identities = 35/41 (85%)
Strand = Plus / Minus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 305 ttgtcaagaaattaggagcacgaggtgtcttcgaagtctac 265
>gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_F02_L04, mRNA sequence
Length = 538
Score = 40.1 bits (20), Expect = 0.005
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 887 ggagggaaggtgatcatcat 906
||||||||||||||||||||
Sbjct: 79 ggagggaaggtgatcatcat 98
Score = 34.2 bits (17), Expect = 0.31
Identities = 35/41 (85%)
Strand = Plus / Plus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 251 ttgtcaagaaattaggagcacgaggtgtcttcgaagtctac 291
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 40.1 bits (20), Expect = 0.005
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 887 ggagggaaggtgatcatcat 906
||||||||||||||||||||
Sbjct: 183 ggagggaaggtgatcatcat 202
Score = 34.2 bits (17), Expect = 0.31
Identities = 35/41 (85%)
Strand = Plus / Plus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtcatcgaggtctac 1099
||||||||||| | ||||| || |||||| |||| ||||||
Sbjct: 355 ttgtcaagaaattaggagcacgaggtgtcttcgaagtctac 395
>gb|CN815015.1|CN815015 HRO4504_H10_P20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_H10_P20, mRNA sequence
Length = 421
Score = 36.2 bits (18), Expect = 0.077
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 964 ggacatgctcatgatggt 981
||||||||||||||||||
Sbjct: 208 ggacatgctcatgatggt 191
>gb|CN817527.1|CN817527 HRO4464_A04_A08ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4464_A04_A08, mRNA sequence
Length = 379
Score = 36.2 bits (18), Expect = 0.077
Identities = 27/30 (90%)
Strand = Plus / Plus
Query: 952 ccagctgctgatggacatgctcatgatggt 981
|||||| |||||||||||| |||||||||
Sbjct: 57 ccagcttctgatggacatgtgcatgatggt 86
>gb|CN820179.1|CN820179 HRO4412_A09_B18ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_A09_B18, mRNA
sequence
Length = 547
Score = 36.2 bits (18), Expect = 0.077
Identities = 27/30 (90%)
Strand = Plus / Plus
Query: 952 ccagctgctgatggacatgctcatgatggt 981
|||||| |||||||||||| |||||||||
Sbjct: 147 ccagcttctgatggacatgtgcatgatggt 176
>gb|CN814931.1|CN814931 HRO4504_A09_B18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_A09_B18, mRNA sequence
Length = 601
Score = 34.2 bits (17), Expect = 0.31
Identities = 26/29 (89%)
Strand = Plus / Plus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtc 1087
|||| |||||| | |||||||||||||||
Sbjct: 146 ttgtgaagaaactgggagctcgtggtgtc 174
>gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_C09_F18, mRNA sequence
Length = 648
Score = 34.2 bits (17), Expect = 0.31
Identities = 26/29 (89%)
Strand = Plus / Plus
Query: 1059 ttgtcaagaaaatcggagctcgtggtgtc 1087
|||| |||||| | |||||||||||||||
Sbjct: 329 ttgtgaagaaactgggagctcgtggtgtc 357
>gb|CN819744.1|CN819744 HRO4403_F04_L07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4403_F04_L07, mRNA
sequence
Length = 664
Score = 34.2 bits (17), Expect = 0.31
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 216 cgctgtccctgccagcg 232
|||||||||||||||||
Sbjct: 418 cgctgtccctgccagcg 434
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 3267
Number of Sequences: 8143
Number of extensions: 3267
Number of successful extensions: 890
Number of sequences better than 0.5: 15
Number of HSP's better than 0.5 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 854
Number of HSP's gapped (non-prelim): 35
length of query: 1327
length of database: 4,702,463
effective HSP length: 16
effective length of query: 1311
effective length of database: 4,572,175
effective search space: 5994121425
effective search space used: 5994121425
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)