BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405245.2.2
(616 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816459.1|CN816459 HRO4521_A01_A01ZS5 Lib AA071E1X Aven... 202 3e-052
gb|CN815174.1|CN815174 HRO4506_F11_K22ZS5 Lib AA071E1X Aven... 36 0.035
gb|CN816722.1|CN816722 HRO4524_A07_B14ZS5 Lib AA071E1X Aven... 36 0.035
>gb|CN816459.1|CN816459 HRO4521_A01_A01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_A01_A01, mRNA sequence
Length = 435
Score = 202 bits (102), Expect = 3e-052
Identities = 174/198 (87%)
Strand = Plus / Plus
Query: 86 tgcatcctgaccgagtgggacgagttcaagaccctggactaccagaagatcttcgacaac 145
|||||||| ||||||||||| |||||||||| |||||||||||||||||||||||||||
Sbjct: 14 tgcatcctcaccgagtgggaggagttcaagaagctggactaccagaagatcttcgacaac 73
Query: 146 atgcagaagcccgccttcgtcttcgacggccgcaacatcatcgacccggagaagttgagg 205
||||||||||||||||| ||||| || || |||||| | | || | |||||| | |||
Sbjct: 74 atgcagaagcccgcctttgtctttgatgggcgcaacgttgttgatgctgagaagctcagg 133
Query: 206 gagatcggcttcatcgtctactccatcggcaagccgctcgacgcctggcttaaggacatg 265
||||| |||||||||| ||||||||||||| |||||||||| || ||||| |||||||||
Sbjct: 134 gagattggcttcatcggctactccatcggcgagccgctcgatgcgtggctcaaggacatg 193
Query: 266 cccgcggtcgcttaattc 283
||||| ||||||||||||
Sbjct: 194 cccgctgtcgcttaattc 211
Score = 34.2 bits (17), Expect = 0.14
Identities = 24/25 (96%), Gaps = 1/25 (4%)
Strand = Plus / Plus
Query: 316 aaggaagaggaactggttgaccatt 340
||||||||| |||||||||||||||
Sbjct: 254 aaggaagag-aactggttgaccatt 277
>gb|CN815174.1|CN815174 HRO4506_F11_K22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_F11_K22, mRNA sequence
Length = 584
Score = 36.2 bits (18), Expect = 0.035
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 168 tcgacggccgcaacatca 185
||||||||||||||||||
Sbjct: 121 tcgacggccgcaacatca 138
>gb|CN816722.1|CN816722 HRO4524_A07_B14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4524_A07_B14, mRNA sequence
Length = 491
Score = 36.2 bits (18), Expect = 0.035
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 168 tcgacggccgcaacatca 185
||||||||||||||||||
Sbjct: 25 tcgacggccgcaacatca 42
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1461
Number of Sequences: 8143
Number of extensions: 1461
Number of successful extensions: 590
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 585
Number of HSP's gapped (non-prelim): 5
length of query: 616
length of database: 4,702,463
effective HSP length: 16
effective length of query: 600
effective length of database: 4,572,175
effective search space: 2743305000
effective search space used: 2743305000
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)