BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Aven... 466 e-131
gb|CN819171.1|CN819171 HRO4451_A03_A05ZS5 Lib AA070E1X Aven... 36 0.042
>gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_G10_M19, mRNA sequence
Length = 749
Score = 466 bits (235), Expect = e-131
Identities = 568/679 (83%)
Strand = Plus / Plus
Query: 51 gcaagaattacctgtcaaggatactaactcttggcccaaggtctcctgctattggtaaca 110
|||||||| || ||||||||||||| |||||||| |||| ||||||||||| |||| ||
Sbjct: 11 gcaagaatcacttgtcaaggatactgactcttggtgcaagatctcctgctataggtagca 70
Query: 111 gagaggagcggagtaatacacctatttcaggaaggaagaatatcattgttatttctgtag 170
|||||| |||||||| ||||||| ||| |||| ||| |||| |||||||||||||| |
Sbjct: 71 aagaggaaaggagtaatgcacctatatcatgaagaaagcatataattgttatttctgtgg 130
Query: 171 actctgttaacaaggaaggtctagtcaggataatcagaaatgctattgaggtcatacata 230
|||||||||||||||||| ||||||| |||||||||||||||||||||||| ||||
Sbjct: 131 actctgttaacaaggaagatctagtccggataatcagaaatgctattgaggctgctcata 190
Query: 231 aagaaaacatgtcgggttcaactggttttgtgctgtcaacttccttaacgatatcagaaa 290
||| |||| | ||| |||||||||||| ||||||||||| || |||| |||||||| |
Sbjct: 191 cagagaacacgccggcttcaactggtttcgtgctgtcaacgtcactaacaatatcagaga 250
Query: 291 tacattcactgctactatctggaggcatgcttcccactgattttgatgctttcatctgca 350
| |||||||||| |||||| |||||||| ||| ||| |||||| |||||||||||||
Sbjct: 251 tttgttcactgctagtatctgtaggcatgcatcctgctggttttgacgctttcatctgca 310
Query: 351 atagtgggagtaacatttactatccttcacattccagagaaacgtcaaacaactccaaga 410
|||||||||||| |||||| ||||||||| |||| | | ||| | | || ||||||
Sbjct: 311 atagtgggagtagcatttattatccttcatattcaggtaatacgcccaccagttccaagg 370
Query: 411 ttacatttgcattagatcaaaatcaccagtcacatattgagtatcgttggggaggagaag 470
||||| || | || ||||||||||||| |||||||||||||||||||||||||||||||
Sbjct: 371 ttacacatgtaatacatcaaaatcaccaatcacatattgagtatcgttggggaggagaag 430
Query: 471 gcctaaggaaatatcttgtgaagtgggccacttcagtggtagaaagaaagggaagaacag 530
| ||||| || ||||| ||||| ||||| |||||||||||||||||||||||||||| |
Sbjct: 431 gtctaagaaagtatctagtgaaatgggctacttcagtggtagaaagaaagggaagaattg 490
Query: 531 agagacaaattatttttgaagatccagagcactcttcggcgtattgtcttgcatttagag 590
| || ||||| || ||||||||| | || || ||||| || ||||| | |||||||| ||
Sbjct: 491 aaaggcaaatgatatttgaagattctgaacattcttctgcctattgacatgcatttaaag 550
Query: 591 tggttaatcccaatcatcttcctcctttaaaggagttgaggaagttgatgagaatccaat 650
||| ||||| |||||||| |||||| |||| || ||||||||||||||||| ||||| |
Sbjct: 551 tggacaatccaaatcatctacctcctctaaaagacttgaggaagttgatgaggatccagt 610
Query: 651 ctctccgttgcaatgcattatataaccacagcgctaccagattatctgtagtccctatcc 710
| |||||||| ||||| | ||||||||||| ||||||||| | |||||| |||| |
Sbjct: 611 cgctccgttgtaatgcgctttataaccacagtgctaccagactgtctgtaactactattc 670
Query: 711 acgcatcacgctctcaggc 729
|||| || |||||||||||
Sbjct: 671 acgcgtctcgctctcaggc 689
>gb|CN819171.1|CN819171 HRO4451_A03_A05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4451_A03_A05, mRNA sequence
Length = 361
Score = 36.2 bits (18), Expect = 0.042
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 286 agaaatacattcactgct 303
||||||||||||||||||
Sbjct: 352 agaaatacattcactgct 335
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2101
Number of Sequences: 8143
Number of extensions: 2101
Number of successful extensions: 603
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 598
Number of HSP's gapped (non-prelim): 3
length of query: 737
length of database: 4,702,463
effective HSP length: 16
effective length of query: 721
effective length of database: 4,572,175
effective search space: 3296538175
effective search space used: 3296538175
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)