BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2253513.2.1
         (746 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN815958.1|CN815958  HRO4515_D01_H01ZS5 Lib AA071E1X Aven...   313   1e-085
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4515_D01_H01, mRNA sequence
          Length = 653

 Score =  313 bits (158), Expect = 1e-085
 Identities = 260/294 (88%)
 Strand = Plus / Plus

                                                                       
Query: 88  ggcagaatcagaatgagaacaggacattatggaagctaggaacgctgccccctggcttga 147
           |||||| ||||||||||||| ||  |||||||||| |||||||  |||||||||| || |
Sbjct: 329 ggcagactcagaatgagaaccggttattatggaagttaggaacattgccccctgggttaa 388

                                                                       
Query: 148 tcactttctactcgacaacaaaacctctggagaaatcttggcatgttcttggacttggat 207
           |||| ||||||||||| ||||| |||||||| ||||| |||||||||||||| || || |
Sbjct: 389 tcacattctactcgaccacaaagcctctggacaaatcctggcatgttcttggtctcgggt 448

                                                                       
Query: 208 acaatcctagcatcagcatggaggagattagaaatgctgctgttgtgcatttcaacggca 267
           | ||||| |||||||||||||||||||| ||||| |||||||||||||||||||| || |
Sbjct: 449 ataatccaagcatcagcatggaggagatcagaaacgctgctgttgtgcatttcaatggta 508

                                                                       
Query: 268 acatgaagccctggctggacatcggtatgaaccagttcaggcacctttggacaaagtatg 327
           |||||||||| |||||||| || ||||||||||||||||||||||| |||||||||||||
Sbjct: 509 acatgaagccttggctggatattggtatgaaccagttcaggcacctctggacaaagtatg 568

                                                                 
Query: 328 tggactatgatgactcatatattcgtcaatgcaattttgcaccgccatagaagg 381
           |||| ||||  |||||||  ||||| || |||||||||||||||||||||||||
Sbjct: 569 tggattatggcgactcattcattcgccagtgcaattttgcaccgccatagaagg 622
  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1771
Number of Sequences: 8143
Number of extensions: 1771
Number of successful extensions: 467
Number of sequences better than  0.5: 1
Number of HSP's better than  0.5 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 466
Number of HSP's gapped (non-prelim): 1
length of query: 746
length of database: 4,702,463
effective HSP length: 16
effective length of query: 730
effective length of database: 4,572,175
effective search space: 3337687750
effective search space used: 3337687750
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)