BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.2
(798 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Aven... 137 2e-032
gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Aven... 119 4e-027
>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
Length = 495
Score = 137 bits (69), Expect = 2e-032
Identities = 231/285 (81%)
Strand = Plus / Plus
Query: 201 gaatagcttcctgagagtgaaggtcgtgaacttcgggtcggacaccgttaacctcacggt 260
|||||||||||||||||||||| | |||||||| || |||||| || | |||||| |
Sbjct: 11 gaatagcttcctgagagtgaagattgtgaactttggttcggaccaggtgagcctcacaat 70
Query: 261 ctccacgagcggactcggggccagcgtcaacgcgctgggatcaaccgccaccgtcctcac 320
| | ||| ||| || ||| ||| ||||||||||||| || | ||| || || || ||
Sbjct: 71 cactgcgaccgggcttcaggctagcatcaacgcgctgggttcgaacgctactgttctgac 130
Query: 321 gtctggcaacgtcatggacgagaactccttcagtaacccgaacaaggttgcgccggtgaa 380
||| |||| || ||||| || || ||||||||||||||||||||||||| ||| ||||
Sbjct: 131 gtccagcaatgtgatggatgaaaattccttcagtaacccgaacaaggttgtgcctgtgac 190
Query: 381 aagccagctgagcaacgccgcggagcagatgcaggtcacgctggctcctcactccttcac 440
|||||||||| ||||||| |||||||||||||||||||| || || ||||||||||| |
Sbjct: 191 aagccagctgcgcaacgctgcggagcagatgcaggtcacactccctgctcactccttctc 250
Query: 441 cgccttcgatctggcgctggctgagcccaagcttgtggcggagat 485
| | ||||| || ||||||||| || | ||||||| ||||||||
Sbjct: 251 ctcgttcgaccttgcgctggctcagtcacagcttgtcgcggagat 295
>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_D10_G20, mRNA sequence
Length = 599
Score = 119 bits (60), Expect = 4e-027
Identities = 120/140 (85%)
Strand = Plus / Plus
Query: 346 tccttcagtaacccgaacaaggttgcgccggtgaaaagccagctgagcaacgccgcggag 405
|||||||||||||| |||||||||| ||| |||| |||||||||| ||||||| ||||||
Sbjct: 190 tccttcagtaacccaaacaaggttgtgcctgtgacaagccagctgcgcaacgctgcggag 249
Query: 406 cagatgcaggtcacgctggctcctcactccttcaccgccttcgatctggcgctggctgag 465
|||||||||||||| || || ||||||||||| || | ||||| || ||||||||| ||
Sbjct: 250 cagatgcaggtcacactccctgctcactccttctcctcgttcgaccttgcgctggctcag 309
Query: 466 cccaagcttgtggcggagat 485
| ||||||| ||||||||
Sbjct: 310 tcacagcttgtcgcggagat 329
Score = 71.9 bits (36), Expect = 8e-013
Identities = 72/84 (85%)
Strand = Plus / Plus
Query: 160 agttcgctggccgcgtctgcgatcacgtggcaggatgcagagaatagcttcctgagagtg 219
||||| ||||| || ||||| ||||| ||||||||| | | |||||||||||||||||||
Sbjct: 4 agttctctggcagcatctgctatcacctggcaggattctgggaatagcttcctgagagtg 63
Query: 220 aaggtcgtgaacttcgggtcggac 243
||| | |||||||| || ||||||
Sbjct: 64 aagattgtgaactttggttcggac 87
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1606
Number of Sequences: 8143
Number of extensions: 1606
Number of successful extensions: 401
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 397
Number of HSP's gapped (non-prelim): 4
length of query: 798
length of database: 4,702,463
effective HSP length: 16
effective length of query: 782
effective length of database: 4,572,175
effective search space: 3575440850
effective search space used: 3575440850
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)