BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161200.2.2
(1078 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 353 2e-097
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 226 3e-059
gb|CN819429.1|CN819429 HRO4405_H09_O17ZS5 Lib AA069E1X Aven... 131 1e-030
gb|CN816903.1|CN816903 HRO4526_A11_A22ZS5 Lib AA071E1X Aven... 64 3e-010
gb|CN820338.1|CN820338 HRO4418_C03_E06ZS5 Lib AA069E1X Aven... 64 3e-010
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 353 bits (178), Expect = 2e-097
Identities = 451/542 (83%)
Strand = Plus / Plus
Query: 68 aggacattccacaaccaaaccgacaatgttatgtgctcatatctgcctctattcaattca 127
|||||||||||||||||||| |||||||| |||||||||||| | || ||| ||| |||
Sbjct: 26 aggacattccacaaccaaactgacaatgtcatgtgctcatatttaccagtatacaactca 85
Query: 128 ttcaacttcactaaaggaggactaatacaactcaaccatggaaggcctcagccacttcag 187
||||||||||| ||||||||| | ||||| |||||||||||| |||||||||||||||||
Sbjct: 86 ttcaacttcacgaaaggaggattgatacagctcaaccatggacggcctcagccacttcag 145
Query: 188 tacgctgtcaacgcagctttccttgcttctctatacagcgactatcttgaagctgcagac 247
|| | |||||||||||| |||||||| ||| | || || ||||| || ||| ||
Sbjct: 146 tatgttgtcaacgcagcattccttgcctctttgtatgctgattatctggacagtgctgat 205
Query: 248 acgcctgggtggtactgcggacctaacttctacaccacagaagtcctccgcaagtttgcc 307
|| || ||||||||||| |||||||| ||||| | || || |||||||||||||||||
Sbjct: 206 acaccagggtggtactgtggacctaatttctataaaaccgatgtcctccgcaagtttgca 265
Query: 308 aggtcacagctcgactacatccttggcaagaacccattgaagatgagctacgttgtgggt 367
| ||||||| |||| ||||| || |||||||||||| |||||||||||||| || |||
Sbjct: 266 aagtcacagatcgattacattctaggcaagaacccacaaaagatgagctacgtcgttggt 325
Query: 368 ttcggaaacaagtacccgaagcgccctcatcacagaggcgcgtcgatacctcacaacggc 427
| ||||| |||||||| ||||| |||||||||||| || || || ||||| |||||
Sbjct: 326 tatggaaagaagtaccccaagcgtgttcatcacagaggggcatcaatccctcataacggt 385
Query: 428 gtcaagtatgggtgcaaaggaggttacaagtggagggacacaaagaaggcaaaccctaac 487
||| | || || ||||||||||||| ||| |||||||| | || ||||||||||| ||
Sbjct: 386 gtccactacggatgcaaaggaggtttcaaatggagggagtctaaaaaggcaaacccaaat 445
Query: 488 atccttgtcggagcaatggtggctggccccgacaggcgtgatggctacaaagatgtccgc 547
||||| ||||| |||||||| |||||||| ||||||| |||||||| ||||||| |||||
Sbjct: 446 atcctcgtcggggcaatggttgctggccctgacaggcatgatggcttcaaagatatccgc 505
Query: 548 acgaactacaactacacggagcctactctcgcagcgaatgctggtctggttgcagcgctg 607
|| ||||| || ||||| ||||||||||| ||||| |||||||| |||||||||| |||
Sbjct: 506 acaaactataattacacagagcctactcttgcagcaaatgctggcttggttgcagcactg 565
Query: 608 at 609
||
Sbjct: 566 at 567
Score = 48.1 bits (24), Expect = 2e-005
Identities = 63/76 (82%)
Strand = Plus / Plus
Query: 647 gataagaacaccatcttctctgcgattcctccgatgttcccaacgcccccgccgccgcca 706
|||||||||||||||||||| || |||| || |||||||||| || | || || |||
Sbjct: 608 gataagaacaccatcttctcagctgttcccccaatgttcccaaactccacacccccacca 667
Query: 707 tcagcatggaaaccat 722
||||| ||||||||||
Sbjct: 668 tcagcttggaaaccat 683
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 226 bits (114), Expect = 3e-059
Identities = 264/314 (84%)
Strand = Plus / Plus
Query: 296 cgcaagtttgccaggtcacagctcgactacatccttggcaagaacccattgaagatgagc 355
||||||||||| | ||||||| |||| ||||| || |||||||||||| |||||||||
Sbjct: 11 cgcaagtttgcaaagtcacagatcgattacattctaggcaagaacccacaaaagatgagc 70
Query: 356 tacgttgtgggtttcggaaacaagtacccgaagcgccctcatcacagaggcgcgtcgata 415
|| ||||| |||| ||||| |||||||| ||||| |||||||||||| ||||| ||
Sbjct: 71 tatgttgttggttatggaaagaagtacccaaagcgtgttcatcacagaggggcgtcaatc 130
Query: 416 cctcacaacggcgtcaagtatgggtgcaaaggaggttacaagtggagggacacaaagaag 475
||||| ||||| ||| | || || ||||||||||||| ||| |||||||| | || |||
Sbjct: 131 cctcataacggtgtccactacggatgcaaaggaggtttcaaatggagggagtctaaaaag 190
Query: 476 gcaaaccctaacatccttgtcggagcaatggtggctggccccgacaggcgtgatggctac 535
|||||||| || ||||| |||||||||||||| |||||||| ||||||| |||||||| |
Sbjct: 191 gcaaacccaaatatcctcgtcggagcaatggttgctggccctgacaggcatgatggcttc 250
Query: 536 aaagatgtccgcacgaactacaactacacggagcctactctcgcagcgaatgctggtctg 595
|||||| ||||||| ||||| || ||||| ||||||||||| ||||| |||||||| ||
Sbjct: 251 aaagatatccgcacaaactataattacacagagcctactcttgcagcaaatgctggcttg 310
Query: 596 gttgcagcgctgat 609
|||||||| |||||
Sbjct: 311 gttgcagcactgat 324
Score = 63.9 bits (32), Expect = 3e-010
Identities = 65/76 (85%)
Strand = Plus / Plus
Query: 647 gataagaacaccatcttctctgcgattcctccgatgttcccaacgcccccgccgccgcca 706
|||||||||||||||||||| || |||| || ||||||||||| || || || || |||
Sbjct: 365 gataagaacaccatcttctcagcagttcccccaatgttcccaactccgccacccccacca 424
Query: 707 tcagcatggaaaccat 722
||||| ||||||||||
Sbjct: 425 tcagcttggaaaccat 440
Score = 38.2 bits (19), Expect = 0.016
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 853 ctgtatcaatatgacagatcagtataa 879
||||||| ||||||||||||||||||
Sbjct: 554 ctgtatcggtatgacagatcagtataa 580
>gb|CN819429.1|CN819429 HRO4405_H09_O17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_H09_O17, mRNA
sequence
Length = 698
Score = 131 bits (66), Expect = 1e-030
Identities = 142/166 (85%), Gaps = 1/166 (0%)
Strand = Plus / Plus
Query: 444 aaggaggttacaagtggagggacacaaagaaggcaaaccctaacatccttgtcggagcaa 503
||||||||| ||| |||||||| | || ||||||||||| || ||||| ||||| ||||
Sbjct: 7 aaggaggtttcaa-tggagggagtctaaaaaggcaaacccaaatatcctcgtcggggcaa 65
Query: 504 tggtggctggccccgacaggcgtgatggctacaaagatgtccgcacgaactacaactaca 563
|||| |||||||| ||||||| |||||||| ||||||| ||||||| ||||| || ||||
Sbjct: 66 tggttgctggccctgacaggcatgatggcttcaaagatatccgcacaaactataattaca 125
Query: 564 cggagcctactctcgcagcgaatgctggtctggttgcagcgctgat 609
| ||||||||||| ||||| |||||||| |||||||||| |||||
Sbjct: 126 cagagcctactcttgcagcaaatgctggcttggttgcagcactgat 171
Score = 65.9 bits (33), Expect = 7e-011
Identities = 72/85 (84%)
Strand = Plus / Plus
Query: 647 gataagaacaccatcttctctgcgattcctccgatgttcccaacgcccccgccgccgcca 706
|||||||||||||||||||| || |||| || ||||||||||| || || || || |||
Sbjct: 212 gataagaacaccatcttctcagctgttcccccaatgttcccaactccaccacccccacca 271
Query: 707 tcagcatggaaaccatagagaggca 731
||||| |||||||||| |||||||
Sbjct: 272 tcagcttggaaaccatgaagaggca 296
Score = 40.1 bits (20), Expect = 0.004
Identities = 30/32 (93%), Gaps = 1/32 (3%)
Strand = Plus / Plus
Query: 970 gtgaagttcttttttgtagaaatggtaccttt 1001
|||||||||||||| |||||||||| ||||||
Sbjct: 518 gtgaagttcttttt-gtagaaatggcaccttt 548
>gb|CN816903.1|CN816903 HRO4526_A11_A22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4526_A11_A22, mRNA sequence
Length = 559
Score = 63.9 bits (32), Expect = 3e-010
Identities = 65/76 (85%)
Strand = Plus / Minus
Query: 647 gataagaacaccatcttctctgcgattcctccgatgttcccaacgcccccgccgccgcca 706
|||||||||||||||||||| || |||| || ||||||||||| || || || || |||
Sbjct: 412 gataagaacaccatcttctcagcagttcccccaatgttcccaactccaccacccccacca 353
Query: 707 tcagcatggaaaccat 722
||||| ||||||||||
Sbjct: 352 tcagcttggaaaccat 337
Score = 44.1 bits (22), Expect = 3e-004
Identities = 32/34 (94%), Gaps = 1/34 (2%)
Strand = Plus / Minus
Query: 968 gtgtgaagttcttttttgtagaaatggtaccttt 1001
|||||||||||||||| |||||||||| ||||||
Sbjct: 108 gtgtgaagttcttttt-gtagaaatggcaccttt 76
Score = 40.1 bits (20), Expect = 0.004
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1007 ttgttttatagaagtgactt 1026
||||||||||||||||||||
Sbjct: 65 ttgttttatagaagtgactt 46
>gb|CN820338.1|CN820338 HRO4418_C03_E06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_C03_E06, mRNA
sequence
Length = 431
Score = 63.9 bits (32), Expect = 3e-010
Identities = 65/76 (85%)
Strand = Plus / Plus
Query: 647 gataagaacaccatcttctctgcgattcctccgatgttcccaacgcccccgccgccgcca 706
|||||||||||||||||||| || |||| || ||||||||||| || || || || |||
Sbjct: 27 gataagaacaccatcttctcagcagttcccccaatgttcccaactccgccacccccacca 86
Query: 707 tcagcatggaaaccat 722
||||| ||||||||||
Sbjct: 87 tcagcttggaaaccat 102
Score = 44.1 bits (22), Expect = 3e-004
Identities = 121/150 (80%), Gaps = 3/150 (2%)
Strand = Plus / Plus
Query: 853 ctgtatcaatatgacagatcagtataagatcctctgtcagacatagatcggaagctgtgt 912
||||||| |||||||||||||||||| ||| ||||||| ||| || ||||| |||||
Sbjct: 216 ctgtatcggtatgacagatcagtataa-atcatctgtcaaccatggactggaagttgtgt 274
Query: 913 gcatcgcgtggagctaatttttgggggtcttgttgctattggcactcc-agctcatgtgt 971
||| ||| | |||||||||| || ||||||| |||| | ||| || ||| ||||
Sbjct: 275 gcaatttgtgaaactaatttttgaggtccttgttgttattcatattccgagttcacgtgt 334
Query: 972 gaagttcttttttgtagaaatggtaccttt 1001
||||||| ||||||||||||||| ||||||
Sbjct: 335 gaagttc-tttttgtagaaatggaaccttt 363
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2391
Number of Sequences: 8143
Number of extensions: 2391
Number of successful extensions: 629
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 605
Number of HSP's gapped (non-prelim): 21
length of query: 1078
length of database: 4,702,463
effective HSP length: 16
effective length of query: 1062
effective length of database: 4,572,175
effective search space: 4855649850
effective search space used: 4855649850
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)