BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.707
(919 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816851.1|CN816851 HRO4525_E06_I11ZS5 Lib AA071E1X Aven... 268 8e-072
gb|CN814915.1|CN814915 HRO4503_H05_P09ZS5 Lib AA071E1X Aven... 262 5e-070
gb|CN819420.1|CN819420 HRO4405_G10_M19ZS5 Lib AA069E1X Aven... 250 2e-066
gb|CN816312.1|CN816312 HRO4519_C11_F21ZS5 Lib AA071E1X Aven... 248 7e-066
gb|CN815987.1|CN815987 HRO4515_F06_L11ZS5 Lib AA071E1X Aven... 157 2e-038
gb|AF118559.1|AF118559 Avena fatua puroindoline precursor, ... 38 0.013
>gb|CN816851.1|CN816851 HRO4525_E06_I11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_E06_I11, mRNA sequence
Length = 698
Score = 268 bits (135), Expect = 8e-072
Identities = 326/389 (83%), Gaps = 3/389 (0%)
Strand = Plus / Minus
Query: 388 actgcctgttcttcttcttgacaccggacttggcaacctggaggctgaggtacaccgagc 447
||||||||||||||||||||||||| ||||||||||||||||||||| ||| || | |
Sbjct: 491 actgcctgttcttcttcttgacaccagacttggcaacctggaggctgcggtgaactgcac 432
Query: 448 tcagcctagcaagagctggcttggtcagatcaggcctgtagtagttgtcactaacctggt 507
||||||| |||||| | |||||||||| |||||||||||||||||||| || |||||||
Sbjct: 431 tcagccttgcaagaacaggcttggtcaagtcaggcctgtagtagttgtcgctgacctggt 372
Query: 508 ttttcacagccttggccatcttgcggaactccttgcgcatcacagacttgtggctgagct 567
| || ||||||||||||||||||||||||||||||||||| |||| ||||||| |||
Sbjct: 371 tcttgacagccttggccatcttgcggaactccttgcgcatgacagtcttgtgggtgaagg 312
Query: 568 tcgcaggtgcgttctgcttcttggtcttggtagtagagaggaccacagccgagtcctt-- 625
|| || |||||||||||| |||||||| || ||||||||||||| | |||||
Sbjct: 311 cagcggggttgttctgcttctttgtcttggtcgtggagaggaccacagtcatctccttgt 252
Query: 626 -tgcagctggctggatcgtcacagtcttcttgttcgccaaacccgagtgtttgaaggagt 684
||||| ||||| | ||||| ||||||||||| |||| || |||| ||| ||||||
Sbjct: 251 cagcagcaggctgcacagtcactgtcttcttgtttgccaggccagagtacttgtaggagt 192
Query: 685 ggatgttgtagaggttgttcggctccttggtgaactgcaccttggcattgccgttgccga 744
||| |||||||||||||| ||||||||| ||||||| |||||||| |||| |||||| |
Sbjct: 191 ggacattgtagaggttgttgggctccttgctgaactgaaccttggcgttgctgttgccaa 132
Query: 745 actgcttcacaaggaaggagttgttcttc 773
||||||| | |||||| |||||||||||
Sbjct: 131 actgcttgatcaggaagcagttgttcttc 103
>gb|CN814915.1|CN814915 HRO4503_H05_P09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_H05_P09, mRNA sequence
Length = 725
Score = 262 bits (132), Expect = 5e-070
Identities = 341/410 (83%), Gaps = 3/410 (0%)
Strand = Plus / Minus
Query: 388 actgcctgttcttcttcttgacaccggacttggcaacctggaggctgaggtacaccgagc 447
||||||||||||||||||| ||||| ||||||||||||||||||||| ||| || | |
Sbjct: 499 actgcctgttcttcttcttaacaccagacttggcaacctggaggctgcggtgaactgcac 440
Query: 448 tcagcctagcaagagctggcttggtcagatcaggcctgtagtagttgtcactaacctggt 507
||||||| || ||||| |||||||||| |||||||||||||||||||| || |||||||
Sbjct: 439 tcagccttgcgagagcaggcttggtcaagtcaggcctgtagtagttgtcgctgacctggt 380
Query: 508 ttttcacagccttggccatcttgcggaactccttgcgcatcacagacttgtggctgagct 567
| || || |||||||||||||||||||||||||||||||| |||| ||||||| |||
Sbjct: 379 tcttgacggccttggccatcttgcggaactccttgcgcatgacagtcttgtgggtgaagg 320
Query: 568 tcgcaggtgcgttctgcttcttggtcttggtagtagagaggaccacagccgagtcctt-- 625
|| ||| |||||||||||| |||||||| || ||||||||||||| | |||||
Sbjct: 319 cagcgggtttgttctgcttctttgtcttggtcgtggagaggaccacagtcatctccttat 260
Query: 626 -tgcagctggctggatcgtcacagtcttcttgttcgccaaacccgagtgtttgaaggagt 684
||||| ||||| | ||||| ||||||||||| |||| || |||| ||| ||||||
Sbjct: 259 cagcagccggctgcacagtcactgtcttcttgtttgccaggccagagtacttgtaggagt 200
Query: 685 ggatgttgtagaggttgttcggctccttggtgaactgcaccttggcattgccgttgccga 744
||| |||||||||||||| ||||||||| ||||||| |||||||| |||| |||||| |
Sbjct: 199 ggacattgtagaggttgttgggctccttgctgaactgaaccttggcgttgctgttgccaa 140
Query: 745 actgcttcacaaggaaggagttgttcttcctcacaacctgccagatcaga 794
||||||| | |||||| ||||||||||| | |||| || ||||| ||||
Sbjct: 139 actgcttgatcaggaagcagttgttcttcttgacaagctcccagaccaga 90
>gb|CN819420.1|CN819420 HRO4405_G10_M19ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_G10_M19, mRNA
sequence
Length = 520
Score = 250 bits (126), Expect = 2e-066
Identities = 198/222 (89%)
Strand = Plus / Minus
Query: 389 ctgcctgttcttcttcttgacaccggacttggcaacctggaggctgaggtacaccgagct 448
|||||||||||||||||||| || ||||||| ||||||||||||| ||||||| |||||
Sbjct: 311 ctgcctgttcttcttcttgatgccagacttggaaacctggaggctgcggtacacagagct 252
Query: 449 cagcctagcaagagctggcttggtcagatcaggcctgtagtagttgtcactaacctggtt 508
|||||| |||||||| ||| ||||||||||||||||||||||||||| | |||||||||
Sbjct: 251 cagccttgcaagagccggcgtggtcagatcaggcctgtagtagttgttgcaaacctggtt 192
Query: 509 tttcacagccttggccatcttgcggaactccttgcgcatcacagacttgtggctgagctt 568
|| ||||||||||||||||||||||||||||||||||||||| |||||| ||||||
Sbjct: 191 cttgacagccttggccatcttgcggaactccttgcgcatcacattcttgtgctggagctt 132
Query: 569 cgcaggtgcgttctgcttcttggtcttggtagtagagaggac 610
| ||| ||||||||||||||||||||||| || ||||||||
Sbjct: 131 agaaggggcgttctgcttcttggtcttggtcgtggagaggac 90
>gb|CN816312.1|CN816312 HRO4519_C11_F21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4519_C11_F21, mRNA sequence
Length = 509
Score = 248 bits (125), Expect = 7e-066
Identities = 203/229 (88%)
Strand = Plus / Minus
Query: 389 ctgcctgttcttcttcttgacaccggacttggcaacctggaggctgaggtacaccgagct 448
|||||||||||||||||||| || ||||||| ||||||||||||| |||| || |||||
Sbjct: 249 ctgcctgttcttcttcttgatgccagacttggaaacctggaggctgcggtagacagagct 190
Query: 449 cagcctagcaagagctggcttggtcagatcaggcctgtagtagttgtcactaacctggtt 508
|||||| |||||||| ||||||||||||||||||||||||||||||| | |||||||||
Sbjct: 189 cagccttgcaagagccggcttggtcagatcaggcctgtagtagttgttgcaaacctggtt 130
Query: 509 tttcacagccttggccatcttgcggaactccttgcgcatcacagacttgtggctgagctt 568
|| || |||||||||||||||||||||||||||||||||||| |||||| ||||||
Sbjct: 129 cttgacggccttggccatcttgcggaactccttgcgcatcacattcttgtgctggagctt 70
Query: 569 cgcaggtgcgttctgcttcttggtcttggtagtagagaggaccacagcc 617
||||| |||||||||||||||||||| || || |||||||| ||||||
Sbjct: 69 agcaggggcgttctgcttcttggtctttgtcgtggagaggacgacagcc 21
>gb|CN815987.1|CN815987 HRO4515_F06_L11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F06_L11, mRNA sequence
Length = 419
Score = 157 bits (79), Expect = 2e-038
Identities = 121/135 (89%)
Strand = Plus / Minus
Query: 389 ctgcctgttcttcttcttgacaccggacttggcaacctggaggctgaggtacaccgagct 448
|||||||||||||||||||| || ||||||| ||||||||||||| ||||||| |||||
Sbjct: 135 ctgcctgttcttcttcttgatgccagacttggaaacctggaggctgcggtacacagagct 76
Query: 449 cagcctagcaagagctggcttggtcagatcaggcctgtagtagttgtcactaacctggtt 508
|||||| |||||||| ||||||||||||||||||||||||||||||| | |||| ||||
Sbjct: 75 cagccttgcaagagccggcttggtcagatcaggcctgtagtagttgttgcaaaccaggtt 16
Query: 509 tttcacagccttggc 523
|| |||||||||||
Sbjct: 15 cttgacagccttggc 1
>gb|AF118559.1|AF118559 Avena fatua puroindoline precursor, mRNA, complete cds
Length = 571
Score = 38.2 bits (19), Expect = 0.013
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 901 tacctgcccgggcggccgc 919
|||||||||||||||||||
Sbjct: 23 tacctgcccgggcggccgc 5
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2447
Number of Sequences: 8143
Number of extensions: 2447
Number of successful extensions: 689
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 673
Number of HSP's gapped (non-prelim): 14
length of query: 919
length of database: 4,702,463
effective HSP length: 16
effective length of query: 903
effective length of database: 4,572,175
effective search space: 4128674025
effective search space used: 4128674025
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)