BLASTX 2.2.6 [Apr-09-2003]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748535.2.1
(619 letters)
Database: /db/trembl-ebi/tmp/swall
1,242,019 sequences; 397,940,306 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sptr|O49215|O49215 (S)-adenosyl-L-methionine:delta 24-sterol met... 191 1e-58
sptr|P93852|P93852 Endosperm C-24 sterol methyltransferase. 191 1e-58
sptr|O82426|O82426 Cycloartenol-C24-methyltransferase. 185 1e-57
sptr|O24328|O24328 S-adenosyl-methionine-sterol-C-methyltransfer... 178 4e-53
sptr|Q43445|Q43445 S-adenosyl-L-methionine:delta24-sterol-C- met... 184 2e-52
sptr|Q41586|Q41586 Delta-24-sterol methyltransferase. 189 1e-50
sptr|Q41587|Q41587 Delta-24-sterol methyltransferase. 189 1e-50
sptr|Q84M50|Q84M50 Putative endosperm C-24 sterol methyltransfer... 196 1e-50
sptr|Q8LKW1|Q8LKW1 Cephalopod. 181 1e-50
sptr|O82434|O82434 S-adenosyl-methionine cycloartenol-C24-methyl... 175 2e-50
sptr|Q9LM02|Q9LM02 SAM:cycloartenol-C24-methyltransferase (24-st... 180 2e-50
sptr|O82720|O82720 S-adenosyl-methionine-sterol-C-methyltransfer... 185 7e-49
sptrnew|EAA36156|EAA36156 Probable DELTA(24)-STEROL C-METHYLTRAN... 159 3e-38
sptr|Q9P3R1|Q9P3R1 Probable DELTA(24)-sterol C-methyltransferase... 159 3e-38
sw|P25087|ERG6_YEAST Sterol 24-C-methyltransferase (EC 2.1.1.41)... 150 1e-35
sw|O14321|ERG6_SCHPO Probable sterol 24-C-methyltransferase (EC ... 146 2e-34
sptr|Q96WX4|Q96WX4 S-adenosyl methionine:sterol methyl transferase. 145 3e-34
sptr|Q9C439|Q9C439 Sterol methyl transferase. 144 6e-34
sw|O74198|ERG6_CANAL Sterol 24-C-methyltransferase (EC 2.1.1.41)... 140 1e-32
sptr|Q875K1|Q875K1 S-adenosylmethionine:D24-methyltransferase. 139 3e-32
sptr|O24153|O24153 S-adenosyl-methionine-sterol-C-methyltransfer... 125 4e-28
sptr|O23655|O23655 S-adenosyl-methionine-sterol-C-methyltransfer... 121 1e-27
sptr|Q94JS4|Q94JS4 At1g76090/T23E18_40. 121 1e-27
sptr|O24154|O24154 S-adenosyl-methionine-sterol-C-methyltransfer... 122 4e-27
sptr|O82427|O82427 24-methylene lophenol C24(1)methyltransferase. 121 6e-27
sptr|Q8H155|Q8H155 Putative sterol-C-methyltransferase. 121 7e-27
sptr|Q8L7L3|Q8L7L3 24-sterol C-methyltransferase. 121 7e-27
sptr|Q9LN25|Q9LN25 F14O10.7 protein (Putative 24-sterol C-methyl... 121 7e-27
sptr|Q39227|Q39227 Sterol-C-methyltransferase. 121 7e-27
sptr|Q8LCB0|Q8LCB0 Sterol-C-methyltransferase. 113 2e-24
>sptr|O49215|O49215 (S)-adenosyl-L-methionine:delta 24-sterol
methyltransferase (EC 2.1.1.41).
Length = 344
Score = 191 bits (484), Expect(3) = 1e-58
Identities = 91/120 (75%), Positives = 101/120 (84%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 78 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 137
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGKEL AG+S C F+K DFM MP DNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 138 RGKELNRLAGISGTCDFVKADFMKMPFDDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 197
Score = 47.0 bits (110), Expect(3) = 1e-58
Identities = 22/33 (66%), Positives = 26/33 (78%)
Frame = +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I+K +VKSAVD+Y KYH
Sbjct: 1 MSKSGALDLASGLGGKINKVEVKSAVDEYEKYH 33
Score = 32.0 bits (71), Expect(3) = 1e-58
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 36 YGGKEEARKSNYTDMVN 52
>sptr|P93852|P93852 Endosperm C-24 sterol methyltransferase.
Length = 344
Score = 191 bits (484), Expect(3) = 1e-58
Identities = 91/120 (75%), Positives = 101/120 (84%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 78 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 137
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGKEL AG+S C F+K DFM MP DNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 138 RGKELNRLAGISGTCDFVKADFMKMPFDDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 197
Score = 47.0 bits (110), Expect(3) = 1e-58
Identities = 22/33 (66%), Positives = 26/33 (78%)
Frame = +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I+K +VKSAVD+Y KYH
Sbjct: 1 MSKSGALDLASGLGGKINKVEVKSAVDEYEKYH 33
Score = 32.0 bits (71), Expect(3) = 1e-58
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 36 YGGKEEARKSNYTDMVN 52
>sptr|O82426|O82426 Cycloartenol-C24-methyltransferase.
Length = 349
Score = 185 bits (470), Expect(3) = 1e-57
Identities = 88/120 (73%), Positives = 100/120 (83%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLG+K GMKVLDVGCGIGGPL EIA+FS S+TGLNNN+Y I+
Sbjct: 78 GESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPLREIAKFSLASVTGLNNNEYQIT 137
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGKEL AG+S C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 138 RGKELNRVAGVSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 197
Score = 48.5 bits (114), Expect(3) = 1e-57
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M++SGA+ LA GG I+KD+VKSAVD+Y KYH
Sbjct: 1 MSRSGAMDLASGLGGKITKDEVKSAVDEYEKYH 33
Score = 32.0 bits (71), Expect(3) = 1e-57
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 36 YGGKEEARKSNYTDMVN 52
>sptr|O24328|O24328
S-adenosyl-methionine-sterol-C-methyltransferase.
Length = 346
Score = 178 bits (452), Expect(3) = 4e-53
Identities = 85/122 (69%), Positives = 99/122 (81%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQLGLK KVLDVGCGIGGPL EI+RFS TS+TGLNNN+Y
Sbjct: 76 FKGESLRESIKRHEHFLALQLGLKPEQKVLDVGCGIGGPLREISRFSLTSVTGLNNNEYQ 135
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGK L AG+ + C F+K DFM MP N+FDA YAI+ATCHAPDA G YSE+YRVL
Sbjct: 136 ITRGKVLNRIAGVDKTCDFVKADFMKMPFPANSFDAVYAIEATCHAPDAYGCYSEIYRVL 195
Query: 614 KP 619
KP
Sbjct: 196 KP 197
Score = 44.3 bits (103), Expect(3) = 4e-53
Identities = 20/34 (58%), Positives = 26/34 (76%)
Frame = +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYHV 203
M+K+GAL LA GG I K++V SAV++Y KYHV
Sbjct: 1 MSKAGALDLASGVGGKIDKNEVLSAVEKYEKYHV 34
Score = 28.1 bits (61), Expect(3) = 4e-53
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GG +E RK+NYTD+++
Sbjct: 36 YGGAEEERKANYTDMVN 52
>sptr|Q43445|Q43445 S-adenosyl-L-methionine:delta24-sterol-C-
methyltransferase.
Length = 367
Score = 184 bits (468), Expect(3) = 2e-52
Identities = 87/122 (71%), Positives = 101/122 (82%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFL LQLGLK G KVLDVGCGIGGPL EI+RFSSTSITGLNNN+Y
Sbjct: 97 WKGESLRESIKRHEHFLPLQLGLKPGQKVLDVGCGIGGPLREISRFSSTSITGLNNNEYQ 156
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL AG+ + C F+K DFM MP DN+FDA YAI+ATCHAPDA G Y E++RVL
Sbjct: 157 ITRGKELNRIAGVDKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVL 216
Query: 614 KP 619
KP
Sbjct: 217 KP 218
Score = 35.0 bits (79), Expect(3) = 2e-52
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +3
Query: 105 AKSGALQLARSTGGSISKDQVKSAVDQYVKYHV 203
++ A+ LA + GG I K +V SAV +Y KYHV
Sbjct: 23 SRLAAMDLASNLGGKIDKAEVLSAVQKYEKYHV 55
Score = 28.9 bits (63), Expect(3) = 2e-52
Identities = 9/17 (52%), Positives = 16/17 (94%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GG++E RK+NYTD+++
Sbjct: 57 YGGQEEERKANYTDMVN 73
>sptr|Q41586|Q41586 Delta-24-sterol methyltransferase.
Length = 363
Score = 189 bits (479), Expect(2) = 1e-50
Identities = 91/120 (75%), Positives = 99/120 (82%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY I+
Sbjct: 97 GESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQIT 156
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGK L S GL C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 157 RGKALNRSVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 216
Score = 33.1 bits (74), Expect(2) = 1e-50
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 55 YGGKEESRKSNYTDMVN 71
>sptr|Q41587|Q41587 Delta-24-sterol methyltransferase.
Length = 363
Score = 189 bits (479), Expect(2) = 1e-50
Identities = 91/120 (75%), Positives = 99/120 (82%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY I+
Sbjct: 97 GESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQIT 156
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGK L S GL C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 157 RGKALNRSVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 216
Score = 33.1 bits (74), Expect(2) = 1e-50
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 55 YGGKEESRKSNYTDMVN 71
>sptr|Q84M50|Q84M50 Putative endosperm C-24 sterol
methyltransferase.
Length = 330
Score = 196 bits (498), Expect(2) = 1e-50
Identities = 96/122 (78%), Positives = 105/122 (86%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GET RE++KRH+HFLALQLGLKKGMKVLDVGCGIGGPL EIARFSS S+TGLNNN Y
Sbjct: 58 WQGETLRESLKRHEHFLALQLGLKKGMKVLDVGCGIGGPLREIARFSSASVTGLNNNAYQ 117
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
ISRGKEL S GLSE C F+K DFM+MPI D TFDAAYAI+ATCHAPDA GVY E+ RVL
Sbjct: 118 ISRGKELNFSVGLSETCNFVKADFMNMPIPDATFDAAYAIEATCHAPDAVGVYREICRVL 177
Query: 614 KP 619
KP
Sbjct: 178 KP 179
Score = 25.8 bits (55), Expect(2) = 1e-50
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 205 LHGGKQESRKSNYTDL 252
++GG+ ESR SNY DL
Sbjct: 17 IYGGEVESRTSNYADL 32
>sptr|Q8LKW1|Q8LKW1 Cephalopod.
Length = 336
Score = 181 bits (458), Expect(3) = 1e-50
Identities = 83/122 (68%), Positives = 101/122 (82%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQLG++ G KVLDVGCGIGGPL EIARFS++++TGLNNN+Y
Sbjct: 70 WKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSAVTGLNNNEYQ 129
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL AG+ + C F+K DFM MP +N FDA YAI+ATCHAPDA G Y E+YRVL
Sbjct: 130 ITRGKELNRLAGVDKTCNFVKADFMKMPFPENNFDAVYAIEATCHAPDAYGCYKEIYRVL 189
Query: 614 KP 619
KP
Sbjct: 190 KP 191
Score = 31.2 bits (69), Expect(3) = 1e-50
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +3
Query: 120 LQLARSTGGSISKDQVKSAVDQYVKYHV 203
+ LA + GG I K V +AV++Y +YHV
Sbjct: 1 MDLASNLGGKIDKSDVLTAVEKYEQYHV 28
Score = 30.8 bits (68), Expect(3) = 1e-50
Identities = 10/17 (58%), Positives = 15/17 (88%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
HGG +E RK+NYTD+++
Sbjct: 30 HGGNEEERKANYTDMVN 46
>sptr|O82434|O82434 S-adenosyl-methionine
cycloartenol-C24-methyltransferase.
Length = 349
Score = 175 bits (443), Expect(3) = 2e-50
Identities = 86/124 (69%), Positives = 100/124 (80%), Gaps = 2/124 (1%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ E+IKR++HFLALQLGLK G KVLDVGCGIGGPL EIARFSSTSITGLNNN+Y
Sbjct: 77 WKGESLLESIKRNEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSITGLNNNEYQ 136
Query: 434 ISRGKELISSAG--LSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYR 607
I+RG+EL + G L++ C F+KGDFM M DN+FDA YAI ATCHAPD G Y E+YR
Sbjct: 137 ITRGQELNNKIGLTLNQTCNFVKGDFMKMSFPDNSFDAVYAIAATCHAPDVVGCYKEIYR 196
Query: 608 VLKP 619
VLKP
Sbjct: 197 VLKP 200
Score = 39.7 bits (91), Expect(3) = 2e-50
Identities = 20/34 (58%), Positives = 25/34 (73%), Gaps = 1/34 (2%)
Frame = +3
Query: 102 MAKSGAL-QLARSTGGSISKDQVKSAVDQYVKYH 200
M+K GAL LA GG I+K++V SAVD+Y KYH
Sbjct: 1 MSKQGALFDLASGVGGKINKEKVLSAVDKYEKYH 34
Score = 27.3 bits (59), Expect(3) = 2e-50
Identities = 8/17 (47%), Positives = 16/17 (94%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GG++E RK+NY+D+++
Sbjct: 37 YGGEEEERKNNYSDMVN 53
>sptr|Q9LM02|Q9LM02 SAM:cycloartenol-C24-methyltransferase
(24-sterol C-methyltransferase) (Sterol
methyltransferase SMT1).
Length = 336
Score = 180 bits (456), Expect(3) = 2e-50
Identities = 83/122 (68%), Positives = 101/122 (82%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQLG++ G KVLDVGCGIGGPL EIARFS++ +TGLNNN+Y
Sbjct: 70 WKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNEYQ 129
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL AG+ + C F+K DFM MP +N+FDA YAI+ATCHAPDA G Y E+YRVL
Sbjct: 130 ITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVL 189
Query: 614 KP 619
KP
Sbjct: 190 KP 191
Score = 31.2 bits (69), Expect(3) = 2e-50
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +3
Query: 120 LQLARSTGGSISKDQVKSAVDQYVKYHV 203
+ LA + GG I K V +AV++Y +YHV
Sbjct: 1 MDLASNLGGKIDKSDVLTAVEKYEQYHV 28
Score = 30.8 bits (68), Expect(3) = 2e-50
Identities = 10/17 (58%), Positives = 15/17 (88%)
Frame = +1
Query: 208 HGGKQESRKSNYTDLIS 258
HGG +E RK+NYTD+++
Sbjct: 30 HGGNEEERKANYTDMVN 46
>sptr|O82720|O82720
S-adenosyl-methionine-sterol-C-methyltransferase.
Length = 346
Score = 185 bits (469), Expect(2) = 7e-49
Identities = 87/122 (71%), Positives = 100/122 (81%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ +E+IKRH+HFLALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y
Sbjct: 76 WKGESLQESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQ 135
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
ISRG+ L GL + C F+KGDFM MP DN+FDA YAI+ATCHAPD G Y E+YRVL
Sbjct: 136 ISRGQVLNRKVGLDQTCNFVKGDFMKMPFPDNSFDAVYAIEATCHAPDPLGCYKEIYRVL 195
Query: 614 KP 619
KP
Sbjct: 196 KP 197
Score = 43.1 bits (100), Expect = 0.003
Identities = 19/33 (57%), Positives = 24/33 (72%)
Frame = +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+K GA LA GG I+K++V SAVD+Y KYH
Sbjct: 1 MSKQGAFDLASGVGGKINKEEVLSAVDKYEKYH 33
Score = 30.8 bits (68), Expect(2) = 7e-49
Identities = 13/49 (26%), Positives = 27/49 (55%)
Frame = +1
Query: 112 QGRYNLLGAPEGRLARTKSNPPLISMSNTTYLHGGKQESRKSNYTDLIS 258
QG ++L G++ + + + +GG++E RK+NYTD+++
Sbjct: 4 QGAFDLASGVGGKINKEEVLSAVDKYEKYHGYYGGEEEERKNNYTDMVN 52
>sptrnew|EAA36156|EAA36156 Probable DELTA(24)-STEROL
C-METHYLTRANSFERASE.
Length = 379
Score = 159 bits (401), Expect = 3e-38
Identities = 75/121 (61%), Positives = 89/121 (73%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PGE+ +AI RH+H+LA Q+G+KK MKVLDVGCG+GGP EIA+F+ ITGLNNNDY I
Sbjct: 105 PGESFYQAIARHEHYLAAQIGIKKDMKVLDVGCGVGGPAREIAKFTDAHITGLNNNDYQI 164
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
R GLS Q F+KGDFM M DN+FDA YAI+AT HAP +GVY E+YRVLK
Sbjct: 165 DRATHYAVRDGLSGQLKFVKGDFMQMSFPDNSFDAVYAIEATVHAPKLEGVYGEIYRVLK 224
Query: 617 P 619
P
Sbjct: 225 P 225
>sptr|Q9P3R1|Q9P3R1 Probable DELTA(24)-sterol C-methyltransferase
(ERG6).
Length = 379
Score = 159 bits (401), Expect = 3e-38
Identities = 75/121 (61%), Positives = 89/121 (73%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PGE+ +AI RH+H+LA Q+G+KK MKVLDVGCG+GGP EIA+F+ ITGLNNNDY I
Sbjct: 105 PGESFYQAIARHEHYLAAQIGIKKDMKVLDVGCGVGGPAREIAKFTDAHITGLNNNDYQI 164
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
R GLS Q F+KGDFM M DN+FDA YAI+AT HAP +GVY E+YRVLK
Sbjct: 165 DRATHYAVRDGLSGQLKFVKGDFMQMSFPDNSFDAVYAIEATVHAPKLEGVYGEIYRVLK 224
Query: 617 P 619
P
Sbjct: 225 P 225
>sw|P25087|ERG6_YEAST Sterol 24-C-methyltransferase (EC 2.1.1.41)
(Delta(24)-sterol C- methyltransferase).
Length = 382
Score = 150 bits (379), Expect = 1e-35
Identities = 68/122 (55%), Positives = 89/122 (72%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
Y GE+ +I RH+H+LA + G+++G VLDVGCG+GGP EIARF+ ++ GLNNNDY
Sbjct: 94 YKGESFAASIARHEHYLAYKAGIQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQ 153
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I++ K LS+Q F+KGDFM M +NTFD YAI+ATCHAP +GVYSE+Y+VL
Sbjct: 154 IAKAKYYAKKYNLSDQMDFVKGDFMKMDFEENTFDKVYAIEATCHAPKLEGVYSEIYKVL 213
Query: 614 KP 619
KP
Sbjct: 214 KP 215
>sw|O14321|ERG6_SCHPO Probable sterol 24-C-methyltransferase (EC
2.1.1.41) (Delta(24)-sterol C-methyltransferase).
Length = 378
Score = 146 bits (369), Expect = 2e-34
Identities = 66/122 (54%), Positives = 85/122 (69%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
Y GE ++I RH+H+LA ++G+K G +VLDVGCG+GGP EI F+ ++ GLNNNDY
Sbjct: 100 YKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGPAREITEFTGCNLVGLNNNDYQ 159
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
ISR L ++ F+KGDFM MP DNTFD YAI+AT HAP +GVY E++RVL
Sbjct: 160 ISRCNNYAVKRNLDKKQVFVKGDFMHMPFEDNTFDYVYAIEATVHAPSLEGVYGEIFRVL 219
Query: 614 KP 619
KP
Sbjct: 220 KP 221
>sptr|Q96WX4|Q96WX4 S-adenosyl methionine:sterol methyl transferase.
Length = 377
Score = 145 bits (367), Expect = 3e-34
Identities = 65/119 (54%), Positives = 87/119 (73%)
Frame = +2
Query: 263 ETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHISR 442
E+ +AI RH+H++AL G+++G VLDVGCG+GGP +I+ F+ +I GLNNNDY I R
Sbjct: 105 ESFSQAIARHEHYIALHAGIREGETVLDVGCGVGGPACQISVFTGANIVGLNNNDYQIQR 164
Query: 443 GKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
K GLS++ F+KGDFM MP +N+FD Y+I+AT HAP +GVYSE+YRVLKP
Sbjct: 165 AKYYSEKKGLSDKLKFIKGDFMQMPFPENSFDKIYSIEATIHAPSLEGVYSEIYRVLKP 223
>sptr|Q9C439|Q9C439 Sterol methyl transferase.
Length = 359
Score = 144 bits (364), Expect = 6e-34
Identities = 65/119 (54%), Positives = 87/119 (73%)
Frame = +2
Query: 263 ETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHISR 442
E+ +AI RH+H++AL G+++G VLDVGCG+GGP +I+ F+ +I GLNNNDY I R
Sbjct: 87 ESFSQAIARHEHYIALHAGIREGETVLDVGCGVGGPDCQISVFTGANIVGLNNNDYQIQR 146
Query: 443 GKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
K GLS++ F+KGDFM MP +N+FD Y+I+AT HAP +GVYSE+YRVLKP
Sbjct: 147 AKYYSEKKGLSDKLKFIKGDFMQMPFPENSFDKIYSIEATIHAPSLEGVYSEIYRVLKP 205
>sw|O74198|ERG6_CANAL Sterol 24-C-methyltransferase (EC 2.1.1.41)
(Delta(24)-sterol C- methyltransferase).
Length = 376
Score = 140 bits (353), Expect = 1e-32
Identities = 66/122 (54%), Positives = 81/122 (66%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
Y GE R+A RH+HFLA ++ L + MKVLDVGCG+GGP EI RF+ I GLNNNDY
Sbjct: 98 YKGEAFRQATARHEHFLAHKMNLNENMKVLDVGCGVGGPGREITRFTDCEIVGLNNNDYQ 157
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I R L + ++KGDFM M +FDA YAI+AT HAP +GVYSE+Y+VL
Sbjct: 158 IERANHYAKKYHLDHKLSYVKGDFMQMDFEPESFDAVYAIEATVHAPVLEGVYSEIYKVL 217
Query: 614 KP 619
KP
Sbjct: 218 KP 219
>sptr|Q875K1|Q875K1 S-adenosylmethionine:D24-methyltransferase.
Length = 375
Score = 139 bits (349), Expect = 3e-32
Identities = 63/122 (51%), Positives = 84/122 (68%)
Frame = +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
Y GE R+A RH+HFLA ++ + + M+VLDVGCG+GGP EI RF+ +I GLNNNDY
Sbjct: 98 YKGEAFRQATARHEHFLAHKMNINENMRVLDVGCGVGGPGREICRFTDCTIVGLNNNDYQ 157
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
+ R + L ++ ++KGDFM M +FDA YAI+AT HAP +GVYSE+Y+VL
Sbjct: 158 VERAQYYAKKYKLDDKLSYVKGDFMQMDFEPESFDAVYAIEATVHAPVLEGVYSEIYKVL 217
Query: 614 KP 619
KP
Sbjct: 218 KP 219
>sptr|O24153|O24153 S-adenosyl-methionine-sterol-C-methyltransferase
homolog (Fragment).
Length = 352
Score = 125 bits (314), Expect = 4e-28
Identities = 57/120 (47%), Positives = 78/120 (65%)
Frame = +2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
G+ REA + H+ LG+K G +VLD GCG+GGP+ IA S +I G+ N+Y ++
Sbjct: 96 GKFHREATRLHEEMAVDLLGIKPGARVLDAGCGVGGPMRAIAAHSGANIVGITINEYQVN 155
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
R + AGL QC + G+F+ MP DN+FD Y+I+ATCHAP + VYSE+YRVLKP
Sbjct: 156 RARAHNKKAGLDSQCEVVCGNFLQMPFDDNSFDGVYSIEATCHAPKLEEVYSEIYRVLKP 215
>sptr|O23655|O23655
S-adenosyl-methionine-sterol-C-methyltransferase.
Length = 359
Score = 121 bits (304), Expect(2) = 1e-27
Identities = 53/121 (43%), Positives = 78/121 (64%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LD GCG+GGP+ IA S +TG+ N+Y +
Sbjct: 100 PGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
R K AGL C + G+F+ MP +NTFD AY+I+ATCHAP + VYSE++RV+K
Sbjct: 160 QRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMK 219
Query: 617 P 619
P
Sbjct: 220 P 220
Score = 23.1 bits (48), Expect(2) = 1e-27
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 138 TGGSISKDQVKSAVDQYVKYHVPP 209
+GGSIS ++VK +QY + P
Sbjct: 38 SGGSISAEKVKDNYNQYWSFFRKP 61
>sptr|Q94JS4|Q94JS4 At1g76090/T23E18_40.
Length = 359
Score = 121 bits (304), Expect(2) = 1e-27
Identities = 53/121 (43%), Positives = 78/121 (64%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LD GCG+GGP+ IA S +TG+ N+Y +
Sbjct: 100 PGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
R K AGL C + G+F+ MP +NTFD AY+I+ATCHAP + VYSE++RV+K
Sbjct: 160 QRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMK 219
Query: 617 P 619
P
Sbjct: 220 P 220
Score = 23.1 bits (48), Expect(2) = 1e-27
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 138 TGGSISKDQVKSAVDQYVKYHVPP 209
+GGSIS ++VK +QY + P
Sbjct: 38 SGGSISAEKVKDNYNQYWSFFRKP 61
>sptr|O24154|O24154
S-adenosyl-methionine-sterol-C-methyltransferase.
Length = 357
Score = 122 bits (305), Expect = 4e-27
Identities = 53/121 (43%), Positives = 78/121 (64%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ REA + H+ +G+K G ++LD GCG+GGP+ IA S ++ G+ N+Y +
Sbjct: 101 PGKSHREATRIHEEMAVDLIGVKPGARILDAGCGVGGPMRAIAAHSRANVVGITINEYQV 160
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY E+YRV+K
Sbjct: 161 KRARMHNKKAGLDSLCEVVCGNFLQMPFPDNSFDGAYSIEATCHAPKLEEVYKEIYRVMK 220
Query: 617 P 619
P
Sbjct: 221 P 221
>sptr|O82427|O82427 24-methylene lophenol C24(1)methyltransferase.
Length = 363
Score = 121 bits (304), Expect = 6e-27
Identities = 57/121 (47%), Positives = 81/121 (66%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG + REA + H+ +A L K G ++LDVGCG+GGP+ IA S +++ G+ N+Y +
Sbjct: 105 PGRSHREATRVHEERVADLLQAKPGHRLLDVGCGVGGPMRAIAAHSGSNVVGITINEYQV 164
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL +C + G+F+ MP SD +FD AY+I+ATCHAP Q VY EV+RVLK
Sbjct: 165 NRARAHNRKAGLDSRCEVVCGNFLSMPFSDASFDGAYSIEATCHAPRLQEVYGEVFRVLK 224
Query: 617 P 619
P
Sbjct: 225 P 225
>sptr|Q8H155|Q8H155 Putative sterol-C-methyltransferase.
Length = 361
Score = 121 bits (303), Expect = 7e-27
Identities = 53/121 (43%), Positives = 80/121 (66%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LDVGCG+GGP+ IA S ++ G+ N+Y +
Sbjct: 100 PGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY+E+YRVLK
Sbjct: 160 NRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLK 219
Query: 617 P 619
P
Sbjct: 220 P 220
>sptr|Q8L7L3|Q8L7L3 24-sterol C-methyltransferase.
Length = 361
Score = 121 bits (303), Expect = 7e-27
Identities = 53/121 (43%), Positives = 80/121 (66%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LDVGCG+GGP+ IA S ++ G+ N+Y +
Sbjct: 100 PGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY+E+YRVLK
Sbjct: 160 NRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLK 219
Query: 617 P 619
P
Sbjct: 220 P 220
>sptr|Q9LN25|Q9LN25 F14O10.7 protein (Putative 24-sterol
C-methyltransferase protein) (Putative
sterol-C-methyltransferase).
Length = 361
Score = 121 bits (303), Expect = 7e-27
Identities = 53/121 (43%), Positives = 80/121 (66%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LDVGCG+GGP+ IA S ++ G+ N+Y +
Sbjct: 100 PGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY+E+YRVLK
Sbjct: 160 NRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLK 219
Query: 617 P 619
P
Sbjct: 220 P 220
>sptr|Q39227|Q39227 Sterol-C-methyltransferase.
Length = 361
Score = 121 bits (303), Expect = 7e-27
Identities = 53/121 (43%), Positives = 80/121 (66%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LDVGCG+GGP+ IA S ++ G+ N+Y +
Sbjct: 100 PGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY+E+YRVLK
Sbjct: 160 NRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLK 219
Query: 617 P 619
P
Sbjct: 220 P 220
>sptr|Q8LCB0|Q8LCB0 Sterol-C-methyltransferase.
Length = 361
Score = 113 bits (283), Expect = 2e-24
Identities = 51/121 (42%), Positives = 78/121 (64%)
Frame = +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG++ ++A + H+ + +K G K+LDVG G+GG + IA S ++ G+ N+Y +
Sbjct: 100 PGKSHKDATRLHEEMAVDLIQVKPGQKILDVGXGVGGXMRAIASHSRANVVGITINEYQV 159
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL C + G+F+ MP DN+FD AY+I+ATCHAP + VY+E+YRVLK
Sbjct: 160 NRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLK 219
Query: 617 P 619
P
Sbjct: 220 P 220
Database: /db/trembl-ebi/tmp/swall
Posted date: Sep 12, 2003 8:28 PM
Number of letters in database: 397,940,306
Number of sequences in database: 1,242,019
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 470,902,293
Number of Sequences: 1242019
Number of extensions: 10175538
Number of successful extensions: 30647
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 29504
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30467
length of database: 397,940,306
effective HSP length: 117
effective length of database: 252,624,083
effective search space used: 22230919304
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)